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Protein

MKI67 FHA domain-interacting nucleolar phosphoprotein

Gene

Nifk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MKI67 FHA domain-interacting nucleolar phosphoprotein
Alternative name(s):
Nucleolar protein interacting with the FHA domain of pKI-67
Short name:
mNIFK
Gene namesi
Name:Nifk
Synonyms:Mki67ip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915199. Nifk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000816302 – 317MKI67 FHA domain-interacting nucleolar phosphoproteinAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei203Citrulline1 Publication1
Modified residuei219PhosphoserineBy similarity1
Modified residuei253PhosphoserineBy similarity1
Modified residuei257PhosphothreonineCombined sources1
Modified residuei261PhosphothreonineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Cross-linki293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei301PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity
Citrullinated by PADI4.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ91VE6.
PaxDbiQ91VE6.
PeptideAtlasiQ91VE6.
PRIDEiQ91VE6.

PTM databases

iPTMnetiQ91VE6.
PhosphoSitePlusiQ91VE6.

Expressioni

Tissue specificityi

Expressed in brain, heart, hind limb muscles, intestine, liver, skin and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000026377.
ExpressionAtlasiQ91VE6. baseline and differential.
GenevisibleiQ91VE6. MM.

Interactioni

Subunit structurei

Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.By similarity

Protein-protein interaction databases

BioGridi212560. 2 interactors.
IntActiQ91VE6. 3 interactors.
MINTiMINT-4112776.
STRINGi10090.ENSMUSP00000027626.

Structurei

3D structure databases

ProteinModelPortaliQ91VE6.
SMRiQ91VE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 125RRMPROSITE-ProRule annotationAdd BLAST79

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4208. Eukaryota.
ENOG4111NF9. LUCA.
GeneTreeiENSGT00390000011515.
HOGENOMiHOG000242795.
HOVERGENiHBG057796.
InParanoidiQ91VE6.
KOiK14838.
OMAiGIDYNFP.
OrthoDBiEOG091G0RMN.
PhylomeDBiQ91VE6.
TreeFamiTF315137.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR021043. hNIFK_FHA_Ki67_binding.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12196. hNIFK_binding. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91VE6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGLAGPAKP SLALNPQEDS QFEKALTQIQ GRTKKPQQKK KEKLNRGVVY
60 70 80 90 100
LGHLPSTLSE SHIYNYCAQF GDISRFRLSR SKRTGNSKGY AFVEFESEDV
110 120 130 140 150
AKIVAETMDN YLFGERLLSC KFMPRKKVHK DLFSQRNALF HRPSFPAVKR
160 170 180 190 200
YNRKRGHLQM LKMEYRFKKK EKLLRKKLAA KGIDYSFPSL VLPKPKNIAV
210 220 230 240 250
AHRDSEGNQV LPDQKEGLSG EPRRKEKMMK EDISNNIPKK RKRSRRKKSS
260 270 280 290 300
VDSQGPTPVC TPTFLERRKS QVMEVGGDKD DEIILKLPVP PVKEDTQKTP
310
TSASPGGKRP RKRKSKQ
Length:317
Mass (Da):36,265
Last modified:December 1, 2001 - v1
Checksum:i08CC82E54EA2D580
GO
Isoform 2 (identifier: Q91VE6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-83: Missing.

Note: No experimental confirmation available.
Show »
Length:269
Mass (Da):30,646
Checksum:i4B9F1C6456B66A73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18E → G in BAC36999 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01463436 – 83Missing in isoform 2. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056870 mRNA. Translation: BAB61920.1.
AY030275 mRNA. Translation: AAK38160.1.
AK019209 mRNA. Translation: BAC25583.2.
AK077770 mRNA. Translation: BAC36999.1.
BC058559 mRNA. Translation: AAH58559.1.
CCDSiCCDS15222.1. [Q91VE6-1]
RefSeqiNP_080748.3. NM_026472.4. [Q91VE6-1]
UniGeneiMm.280311.

Genome annotation databases

EnsembliENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377. [Q91VE6-1]
ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377. [Q91VE6-2]
GeneIDi67949.
KEGGimmu:67949.
UCSCiuc007cic.2. mouse. [Q91VE6-1]
uc011wql.1. mouse. [Q91VE6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056870 mRNA. Translation: BAB61920.1.
AY030275 mRNA. Translation: AAK38160.1.
AK019209 mRNA. Translation: BAC25583.2.
AK077770 mRNA. Translation: BAC36999.1.
BC058559 mRNA. Translation: AAH58559.1.
CCDSiCCDS15222.1. [Q91VE6-1]
RefSeqiNP_080748.3. NM_026472.4. [Q91VE6-1]
UniGeneiMm.280311.

3D structure databases

ProteinModelPortaliQ91VE6.
SMRiQ91VE6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212560. 2 interactors.
IntActiQ91VE6. 3 interactors.
MINTiMINT-4112776.
STRINGi10090.ENSMUSP00000027626.

PTM databases

iPTMnetiQ91VE6.
PhosphoSitePlusiQ91VE6.

Proteomic databases

EPDiQ91VE6.
PaxDbiQ91VE6.
PeptideAtlasiQ91VE6.
PRIDEiQ91VE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377. [Q91VE6-1]
ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377. [Q91VE6-2]
GeneIDi67949.
KEGGimmu:67949.
UCSCiuc007cic.2. mouse. [Q91VE6-1]
uc011wql.1. mouse. [Q91VE6-2]

Organism-specific databases

CTDi84365.
MGIiMGI:1915199. Nifk.

Phylogenomic databases

eggNOGiKOG4208. Eukaryota.
ENOG4111NF9. LUCA.
GeneTreeiENSGT00390000011515.
HOGENOMiHOG000242795.
HOVERGENiHBG057796.
InParanoidiQ91VE6.
KOiK14838.
OMAiGIDYNFP.
OrthoDBiEOG091G0RMN.
PhylomeDBiQ91VE6.
TreeFamiTF315137.

Miscellaneous databases

PROiQ91VE6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026377.
ExpressionAtlasiQ91VE6. baseline and differential.
GenevisibleiQ91VE6. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR021043. hNIFK_FHA_Ki67_binding.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12196. hNIFK_binding. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMK67I_MOUSE
AccessioniPrimary (citable) accession number: Q91VE6
Secondary accession number(s): Q8BMV9, Q8BVL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.