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Q91VE6 (MK67I_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
MKI67 FHA domain-interacting nucleolar phosphoprotein
Alternative name(s):
Nucleolar protein interacting with the FHA domain of pKI-67
Short name=mNIFK
Gene names
Name:Nifk
Synonyms:Mki67ip
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis By similarity.

Subcellular location

Nucleusnucleolus. Chromosome By similarity. Note: Localizes to mitotic chromosomes in conjunction with MKI67 By similarity.

Tissue specificity

Expressed in brain, heart, hind limb muscles, intestine, liver, skin and spleen. Ref.2

Post-translational modification

Phosphorylated By similarity.

Citrullinated by PADI4.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q91VE6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q91VE6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-83: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 317316MKI67 FHA domain-interacting nucleolar phosphoprotein
PRO_0000081630

Regions

Domain47 – 12579RRM

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue2031Citrulline
Modified residue2191Phosphoserine By similarity
Modified residue2531Phosphoserine By similarity
Modified residue2571Phosphothreonine By similarity
Modified residue2611Phosphothreonine By similarity
Modified residue2701Phosphoserine Ref.5
Modified residue3011Phosphothreonine By similarity

Natural variations

Alternative sequence36 – 8348Missing in isoform 2.
VSP_014634

Experimental info

Sequence conflict181E → G in BAC36999. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 08CC82E54EA2D580

FASTA31736,265
        10         20         30         40         50         60 
MAGLAGPAKP SLALNPQEDS QFEKALTQIQ GRTKKPQQKK KEKLNRGVVY LGHLPSTLSE 

        70         80         90        100        110        120 
SHIYNYCAQF GDISRFRLSR SKRTGNSKGY AFVEFESEDV AKIVAETMDN YLFGERLLSC 

       130        140        150        160        170        180 
KFMPRKKVHK DLFSQRNALF HRPSFPAVKR YNRKRGHLQM LKMEYRFKKK EKLLRKKLAA 

       190        200        210        220        230        240 
KGIDYSFPSL VLPKPKNIAV AHRDSEGNQV LPDQKEGLSG EPRRKEKMMK EDISNNIPKK 

       250        260        270        280        290        300 
RKRSRRKKSS VDSQGPTPVC TPTFLERRKS QVMEVGGDKD DEIILKLPVP PVKEDTQKTP 

       310 
TSASPGGKRP RKRKSKQ 

« Hide

Isoform 2 [UniParc].

Checksum: 4B9F1C6456B66A73
Show »

FASTA26930,646

References

« Hide 'large scale' references
[1]"A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis."
Takagi M., Sueishi M., Saiwaki T., Kametaka A., Yoneda Y.
J. Biol. Chem. 276:25386-25391(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Ascitic tumor.
[2]"The nifk gene is widely expressed in mouse tissues and is up-regulated in denervated hind limb muscle."
Magnusson C., Norrby M., Libelius R., Tagerud S.
Cell Biol. Int. 27:469-475(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: NMRI.
Tissue: Skeletal muscle.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-125 (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Embryo and Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Olfactory epithelium.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"Citrullination regulates pluripotency and histone H1 binding to chromatin."
Christophorou M.A., Castelo-Branco G., Halley-Stott R.P., Oliveira C.S., Loos R., Radzisheuskaya A., Mowen K.A., Bertone P., Silva J.C., Zernicka-Goetz M., Nielsen M.L., Gurdon J.B., Kouzarides T.
Nature 507:104-108(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: CITRULLINATION AT ARG-203.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB056870 mRNA. Translation: BAB61920.1.
AY030275 mRNA. Translation: AAK38160.1.
AK019209 mRNA. Translation: BAC25583.2.
AK077770 mRNA. Translation: BAC36999.1.
BC058559 mRNA. Translation: AAH58559.1.
CCDSCCDS15222.1. [Q91VE6-1]
RefSeqNP_080748.3. NM_026472.4. [Q91VE6-1]
UniGeneMm.280311.

3D structure databases

ProteinModelPortalQ91VE6.
SMRQ91VE6. Positions 48-122, 251-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid212560. 5 interactions.
IntActQ91VE6. 2 interactions.
MINTMINT-4112776.

PTM databases

PhosphoSiteQ91VE6.

Proteomic databases

MaxQBQ91VE6.
PaxDbQ91VE6.
PRIDEQ91VE6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377. [Q91VE6-1]
ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377. [Q91VE6-2]
GeneID67949.
KEGGmmu:67949.
UCSCuc007cic.2. mouse. [Q91VE6-1]
uc011wql.1. mouse. [Q91VE6-2]

Organism-specific databases

CTD84365.
MGIMGI:1915199. Nifk.

Phylogenomic databases

eggNOGNOG244773.
GeneTreeENSGT00390000011515.
HOGENOMHOG000242795.
HOVERGENHBG057796.
InParanoidQ91VE6.
KOK14838.
OMAGIDYNFP.
OrthoDBEOG7W6WNN.
PhylomeDBQ91VE6.
TreeFamTF315137.

Gene expression databases

ArrayExpressQ91VE6.
BgeeQ91VE6.
GenevestigatorQ91VE6.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR021043. hNIFK_FHA_Ki67_binding.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF12196. hNIFK_binding. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio326050.
PROQ91VE6.
SOURCESearch...

Entry information

Entry nameMK67I_MOUSE
AccessionPrimary (citable) accession number: Q91VE6
Secondary accession number(s): Q8BMV9, Q8BVL4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot