Q91VE6 (MK67I_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MKI67 FHA domain-interacting nucleolar phosphoprotein

Alternative name(s):
Nucleolar protein interacting with the FHA domain of pKI-67
Short name=mNIFK

Gene names

Name:	Mki67ip
Synonyms:	Nifk

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subunit structure	Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis By similarity.
Subcellular location	Nucleus › nucleolus. Chromosome By similarity. Note: Localizes to mitotic chromosomes in conjunction with MKI67 By similarity.
Tissue specificity	Expressed in brain, heart, hind limb muscles, intestine, liver, skin and spleen. Ref.2
Post-translational modification	Phosphorylated By similarity.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	condensed nuclear chromosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from sequence orthology Ref.1. Source: MGI nucleolus Inferred from sequence orthology Ref.1. Source: MGI nucleoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	RNA binding Inferred from sequence orthology Ref.1. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91VE6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91VE6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-83: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 317	316	MKI67 FHA domain-interacting nucleolar phosphoprotein		PRO_0000081630
Regions
	Domain	47 – 125	79	RRM
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	219	1	Phosphoserine By similarity
	Modified residue	253	1	Phosphoserine By similarity
	Modified residue	257	1	Phosphothreonine By similarity
	Modified residue	261	1	Phosphothreonine By similarity
	Modified residue	270	1	Phosphoserine By similarity
	Modified residue	301	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	36 – 83	48	Missing in isoform 2.		VSP_014634
Experimental info
	Sequence conflict	18	1	E → G in BAC36999. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 08CC82E54EA2D580	FASTA	317	36,265
	10 20 30 40 50 60 MAGLAGPAKP SLALNPQEDS QFEKALTQIQ GRTKKPQQKK KEKLNRGVVY LGHLPSTLSE 70 80 90 100 110 120 SHIYNYCAQF GDISRFRLSR SKRTGNSKGY AFVEFESEDV AKIVAETMDN YLFGERLLSC 130 140 150 160 170 180 KFMPRKKVHK DLFSQRNALF HRPSFPAVKR YNRKRGHLQM LKMEYRFKKK EKLLRKKLAA 190 200 210 220 230 240 KGIDYSFPSL VLPKPKNIAV AHRDSEGNQV LPDQKEGLSG EPRRKEKMMK EDISNNIPKK 250 260 270 280 290 300 RKRSRRKKSS VDSQGPTPVC TPTFLERRKS QVMEVGGDKD DEIILKLPVP PVKEDTQKTP 310 TSASPGGKRP RKRKSKQ
	Isoform 2 [UniParc]. Checksum: 4B9F1C6456B66A73	FASTA	269	30,646
	10 20 30 40 50 60 MAGLAGPAKP SLALNPQEDS QFEKALTQIQ GRTKKTGNSK GYAFVEFESE DVAKIVAETM 70 80 90 100 110 120 DNYLFGERLL SCKFMPRKKV HKDLFSQRNA LFHRPSFPAV KRYNRKRGHL QMLKMEYRFK 130 140 150 160 170 180 KKEKLLRKKL AAKGIDYSFP SLVLPKPKNI AVAHRDSEGN QVLPDQKEGL SGEPRRKEKM 190 200 210 220 230 240 MKEDISNNIP KKRKRSRRKK SSVDSQGPTP VCTPTFLERR KSQVMEVGGD KDDEIILKLP 250 260 VPPVKEDTQK TPTSASPGGK RPRKRKSKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis." Takagi M., Sueishi M., Saiwaki T., Kametaka A., Yoneda Y. J. Biol. Chem. 276:25386-25391(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Ascitic tumor.
[2]	"The nifk gene is widely expressed in mouse tissues and is up-regulated in denervated hind limb muscle." Magnusson C., Norrby M., Libelius R., Tagerud S. Cell Biol. Int. 27:469-475(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Strain: NMRI. Tissue: Skeletal muscle.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-125 (ISOFORM 2). Strain: C57BL/6J. Tissue: Embryo and Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB056870 mRNA. Translation: BAB61920.1. AY030275 mRNA. Translation: AAK38160.1. AK019209 mRNA. Translation: BAC25583.2. AK077770 mRNA. Translation: BAC36999.1. BC058559 mRNA. Translation: AAH58559.1.
IPI	IPI00225912. IPI00608122.
RefSeq	NP_080748.3. NM_026472.4.
UniGene	Mm.280311.
3D structure databases
ProteinModelPortal	Q91VE6.
SMR	Q91VE6. Positions 48-122, 251-289.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q91VE6. 1 interaction.
PTM databases
PhosphoSite	Q91VE6.
Proteomic databases
PaxDb	Q91VE6.
PRIDE	Q91VE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377. ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377.
GeneID	67949.
KEGG	mmu:67949.
UCSC	uc007cic.2. mouse. uc011wql.1. mouse.
Organism-specific databases
CTD	84365.
MGI	MGI:1915199. Mki67ip.
Phylogenomic databases
eggNOG	NOG244773.
GeneTree	ENSGT00390000011515.
HOGENOM	HOG000242795.
HOVERGEN	HBG057796.
InParanoid	Q91VE6.
KO	K14838.
OMA	QVRKRIT.
OrthoDB	EOG4MKNGX.
Gene expression databases
ArrayExpress	Q91VE6.
Bgee	Q91VE6.
Genevestigator	Q91VE6.
GermOnline	ENSMUSG00000026377. Mus musculus.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR021043. hNIFK_FHA_Ki67_binding. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF12196. hNIFK_binding. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	326050.
SOURCE	Search...

Entry information

Entry name

MK67I_MOUSE

Accession

Primary (citable) accession number: Q91VE6
Secondary accession number(s): Q8BMV9, Q8BVL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program