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Protein

MKI67 FHA domain-interacting nucleolar phosphoprotein

Gene

Nifk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. poly(A) RNA binding Source: MGI
  3. RNA binding Source: MGI

GO - Biological processi

  1. negative regulation of phosphatase activity Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MKI67 FHA domain-interacting nucleolar phosphoprotein
Alternative name(s):
Nucleolar protein interacting with the FHA domain of pKI-67
Short name:
mNIFK
Gene namesi
Name:Nifk
Synonyms:Mki67ip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915199. Nifk.

Subcellular locationi

Nucleusnucleolus. Chromosome By similarity
Note: Localizes to mitotic chromosomes in conjunction with MKI67.By similarity

GO - Cellular componenti

  1. condensed nuclear chromosome Source: MGI
  2. cytoplasm Source: MGI
  3. nucleolus Source: MGI
  4. nucleoplasm Source: MGI
  5. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 317316MKI67 FHA domain-interacting nucleolar phosphoproteinPRO_0000081630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei203 – 2031Citrulline1 Publication
Modified residuei219 – 2191PhosphoserineBy similarity
Modified residuei253 – 2531PhosphoserineBy similarity
Modified residuei257 – 2571PhosphothreonineBy similarity
Modified residuei261 – 2611PhosphothreonineBy similarity
Modified residuei270 – 2701Phosphoserine1 Publication
Modified residuei301 – 3011PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated.By similarity
Citrullinated by PADI4.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Phosphoprotein

Proteomic databases

MaxQBiQ91VE6.
PaxDbiQ91VE6.
PRIDEiQ91VE6.

PTM databases

PhosphoSiteiQ91VE6.

Expressioni

Tissue specificityi

Expressed in brain, heart, hind limb muscles, intestine, liver, skin and spleen.1 Publication

Gene expression databases

BgeeiQ91VE6.
ExpressionAtlasiQ91VE6. baseline and differential.
GenevestigatoriQ91VE6.

Interactioni

Subunit structurei

Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.By similarity

Protein-protein interaction databases

BioGridi212560. 5 interactions.
IntActiQ91VE6. 2 interactions.
MINTiMINT-4112776.

Structurei

3D structure databases

ProteinModelPortaliQ91VE6.
SMRiQ91VE6. Positions 48-122, 251-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 12579RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG244773.
GeneTreeiENSGT00390000011515.
HOGENOMiHOG000242795.
HOVERGENiHBG057796.
InParanoidiQ91VE6.
KOiK14838.
OMAiGIDYNFP.
OrthoDBiEOG7W6WNN.
PhylomeDBiQ91VE6.
TreeFamiTF315137.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR021043. hNIFK_FHA_Ki67_binding.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12196. hNIFK_binding. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91VE6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGLAGPAKP SLALNPQEDS QFEKALTQIQ GRTKKPQQKK KEKLNRGVVY
60 70 80 90 100
LGHLPSTLSE SHIYNYCAQF GDISRFRLSR SKRTGNSKGY AFVEFESEDV
110 120 130 140 150
AKIVAETMDN YLFGERLLSC KFMPRKKVHK DLFSQRNALF HRPSFPAVKR
160 170 180 190 200
YNRKRGHLQM LKMEYRFKKK EKLLRKKLAA KGIDYSFPSL VLPKPKNIAV
210 220 230 240 250
AHRDSEGNQV LPDQKEGLSG EPRRKEKMMK EDISNNIPKK RKRSRRKKSS
260 270 280 290 300
VDSQGPTPVC TPTFLERRKS QVMEVGGDKD DEIILKLPVP PVKEDTQKTP
310
TSASPGGKRP RKRKSKQ
Length:317
Mass (Da):36,265
Last modified:November 30, 2001 - v1
Checksum:i08CC82E54EA2D580
GO
Isoform 2 (identifier: Q91VE6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-83: Missing.

Note: No experimental confirmation available.

Show »
Length:269
Mass (Da):30,646
Checksum:i4B9F1C6456B66A73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181E → G in BAC36999 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 8348Missing in isoform 2. 1 PublicationVSP_014634Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056870 mRNA. Translation: BAB61920.1.
AY030275 mRNA. Translation: AAK38160.1.
AK019209 mRNA. Translation: BAC25583.2.
AK077770 mRNA. Translation: BAC36999.1.
BC058559 mRNA. Translation: AAH58559.1.
CCDSiCCDS15222.1. [Q91VE6-1]
RefSeqiNP_080748.3. NM_026472.4. [Q91VE6-1]
UniGeneiMm.280311.

Genome annotation databases

EnsembliENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377. [Q91VE6-1]
ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377. [Q91VE6-2]
GeneIDi67949.
KEGGimmu:67949.
UCSCiuc007cic.2. mouse. [Q91VE6-1]
uc011wql.1. mouse. [Q91VE6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056870 mRNA. Translation: BAB61920.1.
AY030275 mRNA. Translation: AAK38160.1.
AK019209 mRNA. Translation: BAC25583.2.
AK077770 mRNA. Translation: BAC36999.1.
BC058559 mRNA. Translation: AAH58559.1.
CCDSiCCDS15222.1. [Q91VE6-1]
RefSeqiNP_080748.3. NM_026472.4. [Q91VE6-1]
UniGeneiMm.280311.

3D structure databases

ProteinModelPortaliQ91VE6.
SMRiQ91VE6. Positions 48-122, 251-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212560. 5 interactions.
IntActiQ91VE6. 2 interactions.
MINTiMINT-4112776.

PTM databases

PhosphoSiteiQ91VE6.

Proteomic databases

MaxQBiQ91VE6.
PaxDbiQ91VE6.
PRIDEiQ91VE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377. [Q91VE6-1]
ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377. [Q91VE6-2]
GeneIDi67949.
KEGGimmu:67949.
UCSCiuc007cic.2. mouse. [Q91VE6-1]
uc011wql.1. mouse. [Q91VE6-2]

Organism-specific databases

CTDi84365.
MGIiMGI:1915199. Nifk.

Phylogenomic databases

eggNOGiNOG244773.
GeneTreeiENSGT00390000011515.
HOGENOMiHOG000242795.
HOVERGENiHBG057796.
InParanoidiQ91VE6.
KOiK14838.
OMAiGIDYNFP.
OrthoDBiEOG7W6WNN.
PhylomeDBiQ91VE6.
TreeFamiTF315137.

Miscellaneous databases

NextBioi326050.
PROiQ91VE6.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VE6.
ExpressionAtlasiQ91VE6. baseline and differential.
GenevestigatoriQ91VE6.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR021043. hNIFK_FHA_Ki67_binding.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12196. hNIFK_binding. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis."
    Takagi M., Sueishi M., Saiwaki T., Kametaka A., Yoneda Y.
    J. Biol. Chem. 276:25386-25391(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Ascitic tumor.
  2. "The nifk gene is widely expressed in mouse tissues and is up-regulated in denervated hind limb muscle."
    Magnusson C., Norrby M., Libelius R., Tagerud S.
    Cell Biol. Int. 27:469-475(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: NMRI.
    Tissue: Skeletal muscle.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-125 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Embryo and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Olfactory epithelium.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: CITRULLINATION AT ARG-203.

Entry informationi

Entry nameiMK67I_MOUSE
AccessioniPrimary (citable) accession number: Q91VE6
Secondary accession number(s): Q8BMV9, Q8BVL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 18, 2005
Last sequence update: November 30, 2001
Last modified: March 3, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.