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Q91VE6

- MK67I_MOUSE

UniProt

Q91VE6 - MK67I_MOUSE

Protein

MKI67 FHA domain-interacting nucleolar phosphoprotein

Gene

Nifk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. RNA binding Source: MGI

    GO - Biological processi

    1. negative regulation of phosphatase activity Source: UniProtKB

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MKI67 FHA domain-interacting nucleolar phosphoprotein
    Alternative name(s):
    Nucleolar protein interacting with the FHA domain of pKI-67
    Short name:
    mNIFK
    Gene namesi
    Name:Nifk
    Synonyms:Mki67ip
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1915199. Nifk.

    Subcellular locationi

    Nucleusnucleolus. Chromosome By similarity
    Note: Localizes to mitotic chromosomes in conjunction with MKI67.By similarity

    GO - Cellular componenti

    1. condensed nuclear chromosome Source: Ensembl
    2. cytoplasm Source: MGI
    3. nucleolus Source: MGI
    4. nucleoplasm Source: Ensembl

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 317316MKI67 FHA domain-interacting nucleolar phosphoproteinPRO_0000081630Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei203 – 2031Citrulline1 Publication
    Modified residuei219 – 2191PhosphoserineBy similarity
    Modified residuei253 – 2531PhosphoserineBy similarity
    Modified residuei257 – 2571PhosphothreonineBy similarity
    Modified residuei261 – 2611PhosphothreonineBy similarity
    Modified residuei270 – 2701Phosphoserine1 Publication
    Modified residuei301 – 3011PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylated.By similarity
    Citrullinated by PADI4.1 Publication

    Keywords - PTMi

    Acetylation, Citrullination, Phosphoprotein

    Proteomic databases

    MaxQBiQ91VE6.
    PaxDbiQ91VE6.
    PRIDEiQ91VE6.

    PTM databases

    PhosphoSiteiQ91VE6.

    Expressioni

    Tissue specificityi

    Expressed in brain, heart, hind limb muscles, intestine, liver, skin and spleen.1 Publication

    Gene expression databases

    ArrayExpressiQ91VE6.
    BgeeiQ91VE6.
    GenevestigatoriQ91VE6.

    Interactioni

    Subunit structurei

    Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.By similarity

    Protein-protein interaction databases

    BioGridi212560. 5 interactions.
    IntActiQ91VE6. 2 interactions.
    MINTiMINT-4112776.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91VE6.
    SMRiQ91VE6. Positions 48-122, 251-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini47 – 12579RRMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG244773.
    GeneTreeiENSGT00390000011515.
    HOGENOMiHOG000242795.
    HOVERGENiHBG057796.
    InParanoidiQ91VE6.
    KOiK14838.
    OMAiGIDYNFP.
    OrthoDBiEOG7W6WNN.
    PhylomeDBiQ91VE6.
    TreeFamiTF315137.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR021043. hNIFK_FHA_Ki67_binding.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF12196. hNIFK_binding. 1 hit.
    PF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q91VE6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGLAGPAKP SLALNPQEDS QFEKALTQIQ GRTKKPQQKK KEKLNRGVVY    50
    LGHLPSTLSE SHIYNYCAQF GDISRFRLSR SKRTGNSKGY AFVEFESEDV 100
    AKIVAETMDN YLFGERLLSC KFMPRKKVHK DLFSQRNALF HRPSFPAVKR 150
    YNRKRGHLQM LKMEYRFKKK EKLLRKKLAA KGIDYSFPSL VLPKPKNIAV 200
    AHRDSEGNQV LPDQKEGLSG EPRRKEKMMK EDISNNIPKK RKRSRRKKSS 250
    VDSQGPTPVC TPTFLERRKS QVMEVGGDKD DEIILKLPVP PVKEDTQKTP 300
    TSASPGGKRP RKRKSKQ 317
    Length:317
    Mass (Da):36,265
    Last modified:December 1, 2001 - v1
    Checksum:i08CC82E54EA2D580
    GO
    Isoform 2 (identifier: Q91VE6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         36-83: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:269
    Mass (Da):30,646
    Checksum:i4B9F1C6456B66A73
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 181E → G in BAC36999. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei36 – 8348Missing in isoform 2. 1 PublicationVSP_014634Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB056870 mRNA. Translation: BAB61920.1.
    AY030275 mRNA. Translation: AAK38160.1.
    AK019209 mRNA. Translation: BAC25583.2.
    AK077770 mRNA. Translation: BAC36999.1.
    BC058559 mRNA. Translation: AAH58559.1.
    CCDSiCCDS15222.1. [Q91VE6-1]
    RefSeqiNP_080748.3. NM_026472.4. [Q91VE6-1]
    UniGeneiMm.280311.

    Genome annotation databases

    EnsembliENSMUST00000027626; ENSMUSP00000027626; ENSMUSG00000026377. [Q91VE6-1]
    ENSMUST00000112688; ENSMUSP00000108308; ENSMUSG00000026377. [Q91VE6-2]
    GeneIDi67949.
    KEGGimmu:67949.
    UCSCiuc007cic.2. mouse. [Q91VE6-1]
    uc011wql.1. mouse. [Q91VE6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB056870 mRNA. Translation: BAB61920.1 .
    AY030275 mRNA. Translation: AAK38160.1 .
    AK019209 mRNA. Translation: BAC25583.2 .
    AK077770 mRNA. Translation: BAC36999.1 .
    BC058559 mRNA. Translation: AAH58559.1 .
    CCDSi CCDS15222.1. [Q91VE6-1 ]
    RefSeqi NP_080748.3. NM_026472.4. [Q91VE6-1 ]
    UniGenei Mm.280311.

    3D structure databases

    ProteinModelPortali Q91VE6.
    SMRi Q91VE6. Positions 48-122, 251-289.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 212560. 5 interactions.
    IntActi Q91VE6. 2 interactions.
    MINTi MINT-4112776.

    PTM databases

    PhosphoSitei Q91VE6.

    Proteomic databases

    MaxQBi Q91VE6.
    PaxDbi Q91VE6.
    PRIDEi Q91VE6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027626 ; ENSMUSP00000027626 ; ENSMUSG00000026377 . [Q91VE6-1 ]
    ENSMUST00000112688 ; ENSMUSP00000108308 ; ENSMUSG00000026377 . [Q91VE6-2 ]
    GeneIDi 67949.
    KEGGi mmu:67949.
    UCSCi uc007cic.2. mouse. [Q91VE6-1 ]
    uc011wql.1. mouse. [Q91VE6-2 ]

    Organism-specific databases

    CTDi 84365.
    MGIi MGI:1915199. Nifk.

    Phylogenomic databases

    eggNOGi NOG244773.
    GeneTreei ENSGT00390000011515.
    HOGENOMi HOG000242795.
    HOVERGENi HBG057796.
    InParanoidi Q91VE6.
    KOi K14838.
    OMAi GIDYNFP.
    OrthoDBi EOG7W6WNN.
    PhylomeDBi Q91VE6.
    TreeFami TF315137.

    Miscellaneous databases

    NextBioi 326050.
    PROi Q91VE6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91VE6.
    Bgeei Q91VE6.
    Genevestigatori Q91VE6.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR021043. hNIFK_FHA_Ki67_binding.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF12196. hNIFK_binding. 1 hit.
    PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis."
      Takagi M., Sueishi M., Saiwaki T., Kametaka A., Yoneda Y.
      J. Biol. Chem. 276:25386-25391(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Ascitic tumor.
    2. "The nifk gene is widely expressed in mouse tissues and is up-regulated in denervated hind limb muscle."
      Magnusson C., Norrby M., Libelius R., Tagerud S.
      Cell Biol. Int. 27:469-475(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: NMRI.
      Tissue: Skeletal muscle.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-125 (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Embryo and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Olfactory epithelium.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. Cited for: CITRULLINATION AT ARG-203.

    Entry informationi

    Entry nameiMK67I_MOUSE
    AccessioniPrimary (citable) accession number: Q91VE6
    Secondary accession number(s): Q8BMV9, Q8BVL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3