Q91VE3 (KLK7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein-7
EC=3.4.21.117

Alternative name(s):
Serine protease 6
Stratum corneum chymotryptic enzyme
Thymopsin

Gene names

Name:	Klk7
Synonyms:	Prss6, Scce

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. SCCE cleaves insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
Catalytic activity	Cleavage of proteins with aromatic side chains in the P1 position.
Enzyme regulation	Inhibited by Zn2+ and Cu2+ at low micromolar concentrations By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in skin and, at lower levels, in lung, kidney, brain, heart and spleen. In skin, expressed in high suprabasal keratinocytes and in the luminal parts of hair follicles. Not detected in liver and skeletal muscle. Ref.5
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	epidermal lamellar body Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 25	4	Activation peptide By similarity		PRO_0000027944
	Chain	26 – 249	224	Kallikrein-7 By similarity		PRO_0000027945
Regions
	Region	26 – 246	221	Serine protease By similarity
Sites
	Active site	66	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	201	1	Charge relay system By similarity
	Site	105	1	Major binding site for inhibitory zinc or copper By similarity
Amino acid modifications
	Disulfide bond	32 ↔ 161		By similarity
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	133 ↔ 235		By similarity
	Disulfide bond	140 ↔ 207		By similarity
	Disulfide bond	172 ↔ 186		By similarity
	Disulfide bond	197 ↔ 222		By similarity
Experimental info
	Sequence conflict	215 – 217	3	VSW → ASR in AAF01139. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q91VE3 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 0D4E380F12D14F87	FASTA	249	27,257
	10 20 30 40 50 60 MGVWLLSLIT VLLSLALETA GQGERIIDGY KCKEGSHPWQ VALLKGNQLH CGGVLVDKYW 70 80 90 100 110 120 VLTAAHCKMG QYQVQLGSDK IGDQSAQKIK ATKSFRHPGY STKTHVNDIM LVRLDEPVKM 130 140 150 160 170 180 SSKVEAVQLP EHCEPPGTSC TVSGWGTTTS PDVTFPSDLM CSDVKLISSR ECKKVYKDLL 190 200 210 220 230 240 GKTMLCAGIP DSKTNTCNGD SGGPLVCNDT LQGLVSWGTY PCGQPNDPGV YTQVCKYKRW VMETMKTHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel cDNA cloning of mouse serine protease, thymopsin." Yamaguchi N. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Thymus.
[2]	"Epidermal overexpression of stratum corneum chymotryptic enzyme in mice; a model for chronic ithchy dermatitis." Hansson L., Baeckman A., Ny A., Edlund M., Edholm E., Tornell J., Wallbrandt P., Egelrud T. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Molecular cloning and tissue expression of the murine analog to human stratum corneum chymotryptic enzyme." Baeckman A., Stranden P., Brattsand M., Hansson L., Egelrud T. J. Invest. Dermatol. 113:152-155(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-234, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Tail.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008371 mRNA. Translation: BAB55604.1. AF339930 Genomic DNA. Translation: AAK69652.1. AK029477 mRNA. Translation: BAC26467.1. AK077406 mRNA. Translation: BAC36787.1. BC027823 mRNA. Translation: AAH27823.1. AF124299 mRNA. Translation: AAF01139.1.
IPI	IPI00126800.
RefSeq	NP_036002.1. NM_011872.2.
UniGene	Mm.251227.
3D structure databases
ProteinModelPortal	Q91VE3.
SMR	Q91VE3. Positions 26-249.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032955.
Protein family/group databases
MEROPS	S01.300.
PTM databases
PhosphoSite	Q91VE3.
Proteomic databases
PaxDb	Q91VE3.
PRIDE	Q91VE3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032955; ENSMUSP00000032955; ENSMUSG00000030713.
GeneID	23993.
KEGG	mmu:23993.
UCSC	uc009gnt.1. mouse.
Organism-specific databases
CTD	5650.
MGI	MGI:1346336. Klk7.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00690000101721.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	Q91VE3.
KO	K08668.
OMA	VLVNERW.
OrthoDB	EOG42V8H2.
Gene expression databases
Bgee	Q91VE3.
Genevestigator	Q91VE3.
GermOnline	ENSMUSG00000030713. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	303901.
SOURCE	Search...

Entry information

Entry name

KLK7_MOUSE

Accession

Primary (citable) accession number: Q91VE3
Secondary accession number(s): Q9R048

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program