Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kallikrein-7

Gene

Klk7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

Catalytic activityi

Cleavage of proteins with aromatic side chains in the P1 position.

Enzyme regulationi

Inhibited by Zn2+ and Cu2+ at low micromolar concentrations. Inhibited by SERPINA12 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay systemBy similarity
Sitei105 – 1051Major binding site for inhibitory zinc or copperBy similarity
Active sitei108 – 1081Charge relay systemBy similarity
Active sitei201 – 2011Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_275864. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiS01.300.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-7 (EC:3.4.21.117)
Alternative name(s):
Serine protease 6
Stratum corneum chymotryptic enzyme
Thymopsin
Gene namesi
Name:Klk7
Synonyms:Prss6, Scce
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1346336. Klk7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Propeptidei22 – 254Activation peptideBy similarityPRO_0000027944
Chaini26 – 249224Kallikrein-7By similarityPRO_0000027945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 161PROSITE-ProRule annotation
Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
Disulfide bondi133 ↔ 235PROSITE-ProRule annotation
Disulfide bondi140 ↔ 207PROSITE-ProRule annotation
Disulfide bondi172 ↔ 186PROSITE-ProRule annotation
Disulfide bondi197 ↔ 222PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ91VE3.
PRIDEiQ91VE3.

PTM databases

PhosphoSiteiQ91VE3.

Expressioni

Tissue specificityi

Expressed in skin and, at lower levels, in lung, kidney, brain, heart and spleen. In skin, expressed in high suprabasal keratinocytes and in the luminal parts of hair follicles. Not detected in liver and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ91VE3.
GenevisibleiQ91VE3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032955.

Structurei

3D structure databases

ProteinModelPortaliQ91VE3.
SMRiQ91VE3. Positions 26-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni26 – 246221Serine proteaseBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ91VE3.
KOiK08668.
OMAiVLVNERW.
OrthoDBiEOG75B84T.
PhylomeDBiQ91VE3.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91VE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVWLLSLIT VLLSLALETA GQGERIIDGY KCKEGSHPWQ VALLKGNQLH
60 70 80 90 100
CGGVLVDKYW VLTAAHCKMG QYQVQLGSDK IGDQSAQKIK ATKSFRHPGY
110 120 130 140 150
STKTHVNDIM LVRLDEPVKM SSKVEAVQLP EHCEPPGTSC TVSGWGTTTS
160 170 180 190 200
PDVTFPSDLM CSDVKLISSR ECKKVYKDLL GKTMLCAGIP DSKTNTCNGD
210 220 230 240
SGGPLVCNDT LQGLVSWGTY PCGQPNDPGV YTQVCKYKRW VMETMKTHR
Length:249
Mass (Da):27,257
Last modified:December 1, 2001 - v1
Checksum:i0D4E380F12D14F87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti215 – 2173VSW → ASR in AAF01139 (PubMed:10469296).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008371 mRNA. Translation: BAB55604.1.
AF339930 Genomic DNA. Translation: AAK69652.1.
AK029477 mRNA. Translation: BAC26467.1.
AK077406 mRNA. Translation: BAC36787.1.
BC027823 mRNA. Translation: AAH27823.1.
AF124299 mRNA. Translation: AAF01139.1.
CCDSiCCDS21183.1.
RefSeqiNP_036002.1. NM_011872.2.
UniGeneiMm.251227.

Genome annotation databases

EnsembliENSMUST00000032955; ENSMUSP00000032955; ENSMUSG00000030713.
GeneIDi23993.
KEGGimmu:23993.
UCSCiuc009gnt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008371 mRNA. Translation: BAB55604.1.
AF339930 Genomic DNA. Translation: AAK69652.1.
AK029477 mRNA. Translation: BAC26467.1.
AK077406 mRNA. Translation: BAC36787.1.
BC027823 mRNA. Translation: AAH27823.1.
AF124299 mRNA. Translation: AAF01139.1.
CCDSiCCDS21183.1.
RefSeqiNP_036002.1. NM_011872.2.
UniGeneiMm.251227.

3D structure databases

ProteinModelPortaliQ91VE3.
SMRiQ91VE3. Positions 26-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032955.

Protein family/group databases

MEROPSiS01.300.

PTM databases

PhosphoSiteiQ91VE3.

Proteomic databases

PaxDbiQ91VE3.
PRIDEiQ91VE3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032955; ENSMUSP00000032955; ENSMUSG00000030713.
GeneIDi23993.
KEGGimmu:23993.
UCSCiuc009gnt.1. mouse.

Organism-specific databases

CTDi5650.
MGIiMGI:1346336. Klk7.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ91VE3.
KOiK08668.
OMAiVLVNERW.
OrthoDBiEOG75B84T.
PhylomeDBiQ91VE3.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiREACT_275864. Degradation of the extracellular matrix.

Miscellaneous databases

NextBioi303901.
PROiQ91VE3.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VE3.
GenevisibleiQ91VE3. MM.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel cDNA cloning of mouse serine protease, thymopsin."
    Yamaguchi N.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Thymus.
  2. "Epidermal overexpression of stratum corneum chymotryptic enzyme in mice; a model for chronic ithchy dermatitis."
    Hansson L., Baeckman A., Ny A., Edlund M., Edholm E., Tornell J., Wallbrandt P., Egelrud T.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Molecular cloning and tissue expression of the murine analog to human stratum corneum chymotryptic enzyme."
    Baeckman A., Stranden P., Brattsand M., Hansson L., Egelrud T.
    J. Invest. Dermatol. 113:152-155(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-234, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Tail.

Entry informationi

Entry nameiKLK7_MOUSE
AccessioniPrimary (citable) accession number: Q91VE3
Secondary accession number(s): Q9R048
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.