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Q91VE3 (KLK7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-7

EC=3.4.21.117
Alternative name(s):
Serine protease 6
Stratum corneum chymotryptic enzyme
Thymopsin
Gene names
Name:Klk7
Synonyms:Prss6, Scce
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

Catalytic activity

Cleavage of proteins with aromatic side chains in the P1 position.

Enzyme regulation

Inhibited by Zn2+ and Cu2+ at low micromolar concentrations. Inhibited by SERPINA12 By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in skin and, at lower levels, in lung, kidney, brain, heart and spleen. In skin, expressed in high suprabasal keratinocytes and in the luminal parts of hair follicles. Not detected in liver and skeletal muscle. Ref.5

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentepidermal lamellar body

Inferred from electronic annotation. Source: Ensembl

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Propeptide22 – 254Activation peptide By similarity
PRO_0000027944
Chain26 – 249224Kallikrein-7 By similarity
PRO_0000027945

Regions

Region26 – 246221Serine protease By similarity

Sites

Active site661Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2011Charge relay system By similarity
Site1051Major binding site for inhibitory zinc or copper By similarity

Amino acid modifications

Disulfide bond32 ↔ 161 By similarity
Disulfide bond51 ↔ 67 By similarity
Disulfide bond133 ↔ 235 By similarity
Disulfide bond140 ↔ 207 By similarity
Disulfide bond172 ↔ 186 By similarity
Disulfide bond197 ↔ 222 By similarity

Experimental info

Sequence conflict215 – 2173VSW → ASR in AAF01139. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q91VE3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 0D4E380F12D14F87

FASTA24927,257
        10         20         30         40         50         60 
MGVWLLSLIT VLLSLALETA GQGERIIDGY KCKEGSHPWQ VALLKGNQLH CGGVLVDKYW 

        70         80         90        100        110        120 
VLTAAHCKMG QYQVQLGSDK IGDQSAQKIK ATKSFRHPGY STKTHVNDIM LVRLDEPVKM 

       130        140        150        160        170        180 
SSKVEAVQLP EHCEPPGTSC TVSGWGTTTS PDVTFPSDLM CSDVKLISSR ECKKVYKDLL 

       190        200        210        220        230        240 
GKTMLCAGIP DSKTNTCNGD SGGPLVCNDT LQGLVSWGTY PCGQPNDPGV YTQVCKYKRW 


VMETMKTHR 

« Hide

References

« Hide 'large scale' references
[1]"A novel cDNA cloning of mouse serine protease, thymopsin."
Yamaguchi N.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Thymus.
[2]"Epidermal overexpression of stratum corneum chymotryptic enzyme in mice; a model for chronic ithchy dermatitis."
Hansson L., Baeckman A., Ny A., Edlund M., Edholm E., Tornell J., Wallbrandt P., Egelrud T.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Head.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Molecular cloning and tissue expression of the murine analog to human stratum corneum chymotryptic enzyme."
Baeckman A., Stranden P., Brattsand M., Hansson L., Egelrud T.
J. Invest. Dermatol. 113:152-155(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-234, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Tail.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB008371 mRNA. Translation: BAB55604.1.
AF339930 Genomic DNA. Translation: AAK69652.1.
AK029477 mRNA. Translation: BAC26467.1.
AK077406 mRNA. Translation: BAC36787.1.
BC027823 mRNA. Translation: AAH27823.1.
AF124299 mRNA. Translation: AAF01139.1.
RefSeqNP_036002.1. NM_011872.2.
UniGeneMm.251227.

3D structure databases

ProteinModelPortalQ91VE3.
SMRQ91VE3. Positions 26-249.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000032955.

Protein family/group databases

MEROPSS01.300.

PTM databases

PhosphoSiteQ91VE3.

Proteomic databases

PaxDbQ91VE3.
PRIDEQ91VE3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032955; ENSMUSP00000032955; ENSMUSG00000030713.
GeneID23993.
KEGGmmu:23993.
UCSCuc009gnt.1. mouse.

Organism-specific databases

CTD5650.
MGIMGI:1346336. Klk7.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117409.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ91VE3.
KOK08668.
OMAVLVNERW.
OrthoDBEOG75B84T.
PhylomeDBQ91VE3.
TreeFamTF331065.

Gene expression databases

BgeeQ91VE3.
GenevestigatorQ91VE3.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio303901.
PROQ91VE3.
SOURCESearch...

Entry information

Entry nameKLK7_MOUSE
AccessionPrimary (citable) accession number: Q91VE3
Secondary accession number(s): Q9R048
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot