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Q91VE3

- KLK7_MOUSE

UniProt

Q91VE3 - KLK7_MOUSE

Protein

Kallikrein-7

Gene

Klk7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

    Catalytic activityi

    Cleavage of proteins with aromatic side chains in the P1 position.

    Enzyme regulationi

    Inhibited by Zn2+ and Cu2+ at low micromolar concentrations. Inhibited by SERPINA12 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Charge relay systemBy similarity
    Sitei105 – 1051Major binding site for inhibitory zinc or copperBy similarity
    Active sitei108 – 1081Charge relay systemBy similarity
    Active sitei201 – 2011Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_199052. Degradation of the extracellular matrix.

    Protein family/group databases

    MEROPSiS01.300.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallikrein-7 (EC:3.4.21.117)
    Alternative name(s):
    Serine protease 6
    Stratum corneum chymotryptic enzyme
    Thymopsin
    Gene namesi
    Name:Klk7
    Synonyms:Prss6, Scce
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1346336. Klk7.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. epidermal lamellar body Source: Ensembl
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Propeptidei22 – 254Activation peptideBy similarityPRO_0000027944
    Chaini26 – 249224Kallikrein-7By similarityPRO_0000027945Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 161PROSITE-ProRule annotation
    Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
    Disulfide bondi133 ↔ 235PROSITE-ProRule annotation
    Disulfide bondi140 ↔ 207PROSITE-ProRule annotation
    Disulfide bondi172 ↔ 186PROSITE-ProRule annotation
    Disulfide bondi197 ↔ 222PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiQ91VE3.
    PRIDEiQ91VE3.

    PTM databases

    PhosphoSiteiQ91VE3.

    Expressioni

    Tissue specificityi

    Expressed in skin and, at lower levels, in lung, kidney, brain, heart and spleen. In skin, expressed in high suprabasal keratinocytes and in the luminal parts of hair follicles. Not detected in liver and skeletal muscle.1 Publication

    Gene expression databases

    BgeeiQ91VE3.
    GenevestigatoriQ91VE3.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000032955.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91VE3.
    SMRiQ91VE3. Positions 26-249.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni26 – 246221Serine proteaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117409.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ91VE3.
    KOiK08668.
    OMAiVLVNERW.
    OrthoDBiEOG75B84T.
    PhylomeDBiQ91VE3.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91VE3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVWLLSLIT VLLSLALETA GQGERIIDGY KCKEGSHPWQ VALLKGNQLH    50
    CGGVLVDKYW VLTAAHCKMG QYQVQLGSDK IGDQSAQKIK ATKSFRHPGY 100
    STKTHVNDIM LVRLDEPVKM SSKVEAVQLP EHCEPPGTSC TVSGWGTTTS 150
    PDVTFPSDLM CSDVKLISSR ECKKVYKDLL GKTMLCAGIP DSKTNTCNGD 200
    SGGPLVCNDT LQGLVSWGTY PCGQPNDPGV YTQVCKYKRW VMETMKTHR 249
    Length:249
    Mass (Da):27,257
    Last modified:December 1, 2001 - v1
    Checksum:i0D4E380F12D14F87
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti215 – 2173VSW → ASR in AAF01139. (PubMed:10469296)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008371 mRNA. Translation: BAB55604.1.
    AF339930 Genomic DNA. Translation: AAK69652.1.
    AK029477 mRNA. Translation: BAC26467.1.
    AK077406 mRNA. Translation: BAC36787.1.
    BC027823 mRNA. Translation: AAH27823.1.
    AF124299 mRNA. Translation: AAF01139.1.
    CCDSiCCDS21183.1.
    RefSeqiNP_036002.1. NM_011872.2.
    UniGeneiMm.251227.

    Genome annotation databases

    EnsembliENSMUST00000032955; ENSMUSP00000032955; ENSMUSG00000030713.
    GeneIDi23993.
    KEGGimmu:23993.
    UCSCiuc009gnt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008371 mRNA. Translation: BAB55604.1 .
    AF339930 Genomic DNA. Translation: AAK69652.1 .
    AK029477 mRNA. Translation: BAC26467.1 .
    AK077406 mRNA. Translation: BAC36787.1 .
    BC027823 mRNA. Translation: AAH27823.1 .
    AF124299 mRNA. Translation: AAF01139.1 .
    CCDSi CCDS21183.1.
    RefSeqi NP_036002.1. NM_011872.2.
    UniGenei Mm.251227.

    3D structure databases

    ProteinModelPortali Q91VE3.
    SMRi Q91VE3. Positions 26-249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000032955.

    Protein family/group databases

    MEROPSi S01.300.

    PTM databases

    PhosphoSitei Q91VE3.

    Proteomic databases

    PaxDbi Q91VE3.
    PRIDEi Q91VE3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032955 ; ENSMUSP00000032955 ; ENSMUSG00000030713 .
    GeneIDi 23993.
    KEGGi mmu:23993.
    UCSCi uc009gnt.1. mouse.

    Organism-specific databases

    CTDi 5650.
    MGIi MGI:1346336. Klk7.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117409.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi Q91VE3.
    KOi K08668.
    OMAi VLVNERW.
    OrthoDBi EOG75B84T.
    PhylomeDBi Q91VE3.
    TreeFami TF331065.

    Enzyme and pathway databases

    Reactomei REACT_199052. Degradation of the extracellular matrix.

    Miscellaneous databases

    NextBioi 303901.
    PROi Q91VE3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91VE3.
    Genevestigatori Q91VE3.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel cDNA cloning of mouse serine protease, thymopsin."
      Yamaguchi N.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Thymus.
    2. "Epidermal overexpression of stratum corneum chymotryptic enzyme in mice; a model for chronic ithchy dermatitis."
      Hansson L., Baeckman A., Ny A., Edlund M., Edholm E., Tornell J., Wallbrandt P., Egelrud T.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Head.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. "Molecular cloning and tissue expression of the murine analog to human stratum corneum chymotryptic enzyme."
      Baeckman A., Stranden P., Brattsand M., Hansson L., Egelrud T.
      J. Invest. Dermatol. 113:152-155(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-234, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Tail.

    Entry informationi

    Entry nameiKLK7_MOUSE
    AccessioniPrimary (citable) accession number: Q91VE3
    Secondary accession number(s): Q9R048
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3