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Protein

Long-chain fatty acid transport protein 4

Gene

Slc27a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (VLCFAs). Indirectly inhibits RPE65 via substrate competition and via production of VLCFA derivatives like lignoceroyl-CoA. Prevents light-induced degeneration of rods and cones.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi243 – 25412AMPSequence analysisAdd
BLAST

GO - Molecular functioni

  • long-chain fatty acid-CoA ligase activity Source: MGI
  • nucleotide binding Source: UniProtKB-KW
  • very long-chain fatty acid-CoA ligase activity Source: MGI

GO - Biological processi

  • fatty acid metabolic process Source: MGI
  • long-chain fatty acid import Source: MGI
  • long-chain fatty acid metabolic process Source: MGI
  • long-chain fatty acid transport Source: MGI
  • medium-chain fatty acid transport Source: MGI
  • response to nutrient Source: Ensembl
  • skin development Source: MGI
  • very long-chain fatty acid catabolic process Source: MGI
  • very long-chain fatty acid metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism, Lipid transport, Transport

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.
ReactomeiR-MMU-804914. Transport of fatty acids.

Protein family/group databases

TCDBi4.C.1.1.1. the proposed fatty acid transporter (fat) family.

Chemistry

SwissLipidsiSLP:000001141.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain fatty acid transport protein 4 (EC:6.2.1.-)
Short name:
FATP-4
Short name:
Fatty acid transport protein 4
Alternative name(s):
Solute carrier family 27 member 4
Gene namesi
Name:Slc27a4
Synonyms:Acsvl4, Fatp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1347347. Slc27a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4223HelicalSequence analysisAdd
BLAST
Transmembranei139 – 15618HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • brush border membrane Source: MGI
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
  • microvillus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Slc27a4 are the cause of wrinkle-free (wrfr) phenotype. It is a spontaneous, autosomal recessive mutation resulting in very tight, thick skin and is secondary characterized by severe breathing difficulties. Mice die shortly after birth. This phenotype is similar to human restrictive dermopathy, a very rare human genetic disorder.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 643643Long-chain fatty acid transport protein 4PRO_0000193211Add
BLAST

Proteomic databases

MaxQBiQ91VE0.
PaxDbiQ91VE0.
PeptideAtlasiQ91VE0.
PRIDEiQ91VE0.

PTM databases

iPTMnetiQ91VE0.
PhosphoSiteiQ91VE0.

Expressioni

Tissue specificityi

Most abundantly expressed in small intestine, brain, kidney, liver, skin and heart. In small intestine, expressed at high levels on the apical side of mature enterocytes. Highly expressed by the epithelial cells of the visceral endoderm and localized to the brush-border membrane of extraembryonic endodermal cells (at protein level). Expressed in the retinal pigment epithelium and in the retina (at protein level). Expressed in the retinal pigment epithelium and in the retina.5 Publications

Gene expression databases

BgeeiENSMUSG00000059316.
GenevisibleiQ91VE0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078971.

Structurei

3D structure databases

ProteinModelPortaliQ91VE0.
SMRiQ91VE0. Positions 74-607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179. Eukaryota.
ENOG410XQ8T. LUCA.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ91VE0.
KOiK08745.
OMAiWRFIRIF.
OrthoDBiEOG091G0B76.
PhylomeDBiQ91VE0.
TreeFamiTF313430.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR030304. FATP4.
IPR022272. Lipocalin_CS.
[Graphical view]
PANTHERiPTHR24096:SF145. PTHR24096:SF145. 1 hit.
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLGASLVGA LLFSKLVLKL PWTQVGFSLL LLYLGSGGWR FIRVFIKTVR
60 70 80 90 100
RDIFGGMVLL KVKTKVRRYL QERKTVPLLF ASMVQRHPDK TALIFEGTDT
110 120 130 140 150
HWTFRQLDEY SSSVANFLQA RGLASGNVVA LFMENRNEFV GLWLGMAKLG
160 170 180 190 200
VEAALINTNL RRDALRHCLD TSKARALIFG SEMASAICEI HASLEPTLSL
210 220 230 240 250
FCSGSWEPST VPVSTEHLDP LLEDAPKHLP SHPDKGFTDK LFYIYTSGTT
260 270 280 290 300
GLPKAAIVVH SRYYRMASLV YYGFRMRPDD IVYDCLPLYH SAGNIVGIGQ
310 320 330 340 350
CLLHGMTVVI RKKFSASRFW DDCIKYNCTI VQYIGELCRY LLNQPPREAE
360 370 380 390 400
SRHKVRMALG NGLRQSIWTD FSSRFHIPQV AEFYGATECN CSLGNFDSRV
410 420 430 440 450
GACGFNSRIL SFVYPIRLVR VNEDTMELIR GPDGVCIPCQ PGQPGQLVGR
460 470 480 490 500
IIQQDPLRRF DGYLNQGANN KKIANDVFKK GDQAYLTGDV LVMDELGYLY
510 520 530 540 550
FRDRTGDTFR WKGENVSTTE VEGTLSRLLH MADVAVYGVE VPGTEGRAGM
560 570 580 590 600
AAVASPISNC DLESFAQTLK KELPLYARPI FLRFLPELHK TGTFKFQKTE
610 620 630 640
LRKEGFDPSV VKDPLFYLDA RKGCYVALDQ EAYTRIQAGE EKL
Length:643
Mass (Da):72,319
Last modified:December 1, 2001 - v1
Checksum:i10B3E9730FDF586A
GO

Sequence cautioni

The sequence AAC40188 differs from that shown.Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence AAC40188 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti330 – 3301I → V in AAC40188 (PubMed:9671728).Curated
Sequence conflicti542 – 5421P → S in BAE41756 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251113 mRNA. Translation: CAC42082.1.
AJ276492 Genomic DNA. Translation: CAC42083.1.
AK036919 mRNA. Translation: BAC29639.1.
AK155388 mRNA. Translation: BAE33236.1.
AK170377 mRNA. Translation: BAE41756.1.
CT010312 mRNA. Translation: CAJ18520.1.
BC023114 mRNA. Translation: AAH23114.1.
AF072759 mRNA. Translation: AAC40188.1. Sequence problems.
CCDSiCCDS15858.1.
RefSeqiNP_036119.1. NM_011989.4.
UniGeneiMm.330113.

Genome annotation databases

EnsembliENSMUST00000080065; ENSMUSP00000078971; ENSMUSG00000059316.
GeneIDi26569.
KEGGimmu:26569.
UCSCiuc008jaf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251113 mRNA. Translation: CAC42082.1.
AJ276492 Genomic DNA. Translation: CAC42083.1.
AK036919 mRNA. Translation: BAC29639.1.
AK155388 mRNA. Translation: BAE33236.1.
AK170377 mRNA. Translation: BAE41756.1.
CT010312 mRNA. Translation: CAJ18520.1.
BC023114 mRNA. Translation: AAH23114.1.
AF072759 mRNA. Translation: AAC40188.1. Sequence problems.
CCDSiCCDS15858.1.
RefSeqiNP_036119.1. NM_011989.4.
UniGeneiMm.330113.

3D structure databases

ProteinModelPortaliQ91VE0.
SMRiQ91VE0. Positions 74-607.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078971.

Chemistry

SwissLipidsiSLP:000001141.

Protein family/group databases

TCDBi4.C.1.1.1. the proposed fatty acid transporter (fat) family.

PTM databases

iPTMnetiQ91VE0.
PhosphoSiteiQ91VE0.

Proteomic databases

MaxQBiQ91VE0.
PaxDbiQ91VE0.
PeptideAtlasiQ91VE0.
PRIDEiQ91VE0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080065; ENSMUSP00000078971; ENSMUSG00000059316.
GeneIDi26569.
KEGGimmu:26569.
UCSCiuc008jaf.1. mouse.

Organism-specific databases

CTDi10999.
MGIiMGI:1347347. Slc27a4.

Phylogenomic databases

eggNOGiKOG1179. Eukaryota.
ENOG410XQ8T. LUCA.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ91VE0.
KOiK08745.
OMAiWRFIRIF.
OrthoDBiEOG091G0B76.
PhylomeDBiQ91VE0.
TreeFamiTF313430.

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.
ReactomeiR-MMU-804914. Transport of fatty acids.

Miscellaneous databases

PROiQ91VE0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059316.
GenevisibleiQ91VE0. MM.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR030304. FATP4.
IPR022272. Lipocalin_CS.
[Graphical view]
PANTHERiPTHR24096:SF145. PTHR24096:SF145. 1 hit.
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS27A4_MOUSE
AccessioniPrimary (citable) accession number: Q91VE0
Secondary accession number(s): O88562, Q3TD48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Slc27a4 deficient mice display features of a neonatally lethal restrictive dermopathy. Their skin is characterized by hyperproliferative hyperkeratosis with a disturbed epidermal barrier, a flat dermal-epidermal junction, a reduced number of pilo-sebaceous structures, and a compact dermis. The rigid skin consistency results in an altered body shape with facial dysmorphia, generalized joint flexion contractures and impaired movement including suckling and breathing deficiencies. Lipid analysis demonstrates a disturbed fatty acid composition of epidermal ceramides, in particular a decrease in the C26:0 and C26:0-OH fatty acid substitutes.
Deletion of Slc27a4 results in embryonic lethality, which has been attributed to a requirement for fat absorption early in embryonic development across the visceral endoderm.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.