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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

Ndufs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi75Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi78Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi92Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi124Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi128Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi131Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi137Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi176Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi179Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi182Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi226Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-MMU-611105 Respiratory electron transport
R-MMU-6799198 Complex I biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
Gene namesi
Name:Ndufs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2443241 Ndufs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23MitochondrionBy similarityAdd BLAST23
ChainiPRO_000001996924 – 727NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialAdd BLAST704

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84N6-acetyllysineCombined sources1
Modified residuei461PhosphoserineCombined sources1
Modified residuei467N6-acetyllysineCombined sources1
Modified residuei499N6-acetyllysineCombined sources1
Modified residuei709N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylation of Lys-84 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ91VD9
MaxQBiQ91VD9
PaxDbiQ91VD9
PeptideAtlasiQ91VD9
PRIDEiQ91VD9

2D gel databases

REPRODUCTION-2DPAGEiIPI00308882
Q91VD9

PTM databases

iPTMnetiQ91VD9
PhosphoSitePlusiQ91VD9
SwissPalmiQ91VD9

Expressioni

Gene expression databases

BgeeiENSMUSG00000025968
CleanExiMM_NDUFS1
ExpressionAtlasiQ91VD9 baseline and differential
GenevisibleiQ91VD9 MM

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.By similarity

Protein-protein interaction databases

BioGridi230599, 4 interactors
CORUMiQ91VD9
IntActiQ91VD9, 7 interactors
MINTiQ91VD9
STRINGi10090.ENSMUSP00000027111

Structurei

3D structure databases

ProteinModelPortaliQ91VD9
SMRiQ91VD9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 1082Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini108 – 1474Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini245 – 3014Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2282 Eukaryota
COG1034 LUCA
GeneTreeiENSGT00390000018768
HOGENOMiHOG000031442
HOVERGENiHBG003482
InParanoidiQ91VD9
KOiK03934
OMAiPQASCAM
OrthoDBiEOG091G02C2
TreeFamiTF105756

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NuoG_C
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
PF09326 NADH_dhqG_C, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91VD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRIPIKRAL IGLSNSPKGY VRTTGTAASN LIEVFVDGQS VMVEPGTTVL
60 70 80 90 100
QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW
110 120 130 140 150
NILTNSEKSK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGSDR
160 170 180 190 200
SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR
210 220 230 240 250
GNDMQVGTYI EKMFMSELSG NVIDICPVGA LTSKPYAFTA RPWETRKTES
260 270 280 290 300
IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ
310 320 330 340 350
RLTEPMVRNE KGLLTYTSWE DALSRVAGML QNFEGNAVAA IAGGLVDAEA
360 370 380 390 400
LVALKDLLNK VDSDNLCTEE IFPTEGAGTD LRSNYLLNTT IAGVEEADVV
410 420 430 440 450
LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY RYDHLGDSPK
460 470 480 490 500
ILQDIASGRH SFCEVLKDAK KPMVVLGSSA LQRDDGAAIL VAVSNMVQKI
510 520 530 540 550
RVTTGVAAEW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPKMLFLLGA
560 570 580 590 600
DGGCITRQDL PKDCFIVYQG HHGDVGAPMA DVILPGAAYT EKSATYVNTE
610 620 630 640 650
GRAQQTKVAV TPPGLAREDW KIIRALSEIA GITLPYDTLD QVRNRLEEVS
660 670 680 690 700
PNLVRYDDIE ETNYFQQASE LAKLVNQEVL ADPLVPPQLT IKDFYMTDSI
710 720
SRASQTMAKC VKAVTEGAQA VEEPSIC
Length:727
Mass (Da):79,777
Last modified:July 27, 2011 - v2
Checksum:i4A4B4BD6C330BB4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti491V → A in AAH06660 (PubMed:15489334).Curated1
Sequence conflicti491V → A in AAH15300 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034597 mRNA Translation: BAE20494.1
AK036926 mRNA Translation: BAC29641.1
AK142711 mRNA Translation: BAE25170.1
AL645950 Genomic DNA Translation: CAI24120.1
CH466548 Genomic DNA Translation: EDL00180.1
CH466548 Genomic DNA Translation: EDL00181.1
CH466548 Genomic DNA Translation: EDL00182.1
CH466548 Genomic DNA Translation: EDL00184.1
BC006660 mRNA Translation: AAH06660.1
BC015300 mRNA Translation: AAH15300.1
CCDSiCCDS14996.1
RefSeqiNP_001153510.1, NM_001160038.1
NP_001153511.1, NM_001160039.1
NP_001153512.1, NM_001160040.1
NP_663493.2, NM_145518.2
XP_006496015.1, XM_006495952.3
UniGeneiMm.290791

Genome annotation databases

EnsembliENSMUST00000027111; ENSMUSP00000027111; ENSMUSG00000025968
ENSMUST00000168099; ENSMUSP00000126621; ENSMUSG00000025968
GeneIDi227197
KEGGimmu:227197
UCSCiuc007bfu.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNDUS1_MOUSE
AccessioniPrimary (citable) accession number: Q91VD9
Secondary accession number(s): Q3UQ73, Q8BM16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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