Reviewed,
UniProtKB/Swiss-Prot Q91VD9 (NDUS1_MOUSE)
Last modified
November 25, 2008.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial EC=1.6.5.3 EC=1.6.99.3 Alternative name(s): Complex I-75kD Short name=CI-75kD | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 727 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity. |
| Catalytic activity | NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor. |
| Cofactor | Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity. |
| Subunit structure | Complex I is composed of 45 different subunits By similarity. |
| Subcellular location | Mitochondrion inner membraneBy similarity. Note= Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity. |
| Post-translational modification | Acetylation of Lys-84 is observed in liver mitochondria from fasted mice but not from fed mice. |
| Sequence similarities | Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 23 | 23 | Mitochondrion By similarity | ||||||
| Chain | 24 – 727 | 704 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | PRO_0000019969 | |||||
Regions | |||||||||
| Domain | 30 – 108 | 79 | 2Fe-2S ferredoxin-type | ||||||
Sites | |||||||||
| Metal binding | 64 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 75 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 78 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 92 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity | ||||||
| Metal binding | 124 | 1 | Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity | ||||||
| Metal binding | 128 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 131 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 137 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 176 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
| Metal binding | 179 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
| Metal binding | 182 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
| Metal binding | 226 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 84 | 1 | N6-acetyllysine | ||||||
Experimental info | |||||||||
| Sequence conflict | 491 | 1 | A → V in BAC29641. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Vagina. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
| [3] | Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 77-84; 88-108; 185-212; 247-266; 277-289; 292-299; 312-325; 361-382; 409-417; 421-441; 451-467; 471-483; 502-511; 519-539; 544-557; 608-617; 625-643; 646-655; 674-702 AND 713-727, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain. |
| [4] | Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 185-200, MASS SPECTROMETRY. Tissue: Hippocampus. |
| [5] | "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-84, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AK036926 mRNA. Translation: BAC29641.1. BC006660 mRNA. Translation: AAH06660.1. BC015300 mRNA. Translation: AAH15300.1. | |
| RefSeq | NP_663493.1. |
| UniGene | Mm.290791 Mm.392955 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q91VD9. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | Q91VD9. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000025968. Mus musculus. [Contig view] |
| GeneID | 227197. |
| KEGG | mmu:227197. |
Organism-specific databases | |
| MGI | MGI:2443241. Ndufs1. |
Phylogenomic databases | |
| HOVERGEN | Q91VD9. |
Gene expression databases | |
| ArrayExpress | Q91VD9. |
| CleanEx | MM_NDUFS1. |
| GermOnline | ENSMUSG00000025968. Mus musculus. |
Family and domain databases | |
| InterPro | IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_DHase_75KDa_su_CS. IPR010228. NADH_quinone_OxRdtase_G. IPR015405. NuoG_C. [Graphical view] |
| Pfam | PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01973. NuoG. 1 hit. |
| PROSITE | PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 378510. |
| SOURCE | Search... |
Entry information
| Entry name | NDUS1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q91VD9 Secondary accession number(s): Q8BM16 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


