Q91VB2 (KCC1G_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium/calmodulin-dependent protein kinase type 1G
EC=2.7.11.17

Alternative name(s):
CaM kinase I gamma
Short name=CaM kinase IG
Short name=CaM-KI gamma
Short name=CaMKI gamma
Short name=CaMKIG
CaMK-like CREB kinase III
Short name=CLICK III

Gene names

Name:

Camk1g

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.1
Enzyme regulation	Activated by Ca2+/calmodulin. Binding of calmodulin is thought to result in a conformational change and leads to activation through phosphorylation by CAMKK1. Ref.1
Subcellular location	Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein Ref.1.
Tissue specificity	Highly expressed in brain, in neuronal cell bodies of the central nucleus of amygdala and ventromedial hypothalamic nucleus. Also detected in heart, testis, and kidney. Ref.1
Developmental stage	Expression starts at E11 in parallel with the onset of development of the central nervous system. Ref.1
Domain	The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate By similarity.
Post-translational modification	May be prenylated on Cys-474.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Golgi apparatus Membrane
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Phosphoprotein Prenylation
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell calcium- and calmodulin-dependent protein kinase complex Inferred from direct assay Ref.1. Source: MGI plasma membrane Inferred from direct assay Ref.1. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Calcium/calmodulin-dependent protein kinase type 1G		PRO_0000086085
Regions
	Domain	23 – 277	255	Protein kinase
	Nucleotide binding	29 – 37	9	ATP By similarity
	Region	277 – 317	41	Autoinhibitory domain By similarity
	Region	297 – 318	22	Calmodulin-binding By similarity
Sites
	Active site	143	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity
Amino acid modifications
	Modified residue	178	1	Phosphothreonine By similarity
Experimental info
	Mutagenesis	474	1	C → S: Loss of association to membranes. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q91VB2 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 3A61FBDC49A7BB56	FASTA	477	53,296
	10 20 30 40 50 60 MGRKEEEDCS SWKKQTTNIR KTFIFMEVLG SGAFSEVFLV KQRVTGKLFA LKCIKKSPAF 70 80 90 100 110 120 RDSSLENEIA VLKRIKHENI VTLEDIYEST THYYLVMQLV SGGELFDRIL ERGVYTEKDA 130 140 150 160 170 180 SLVIQQVLSA VKYLHENGIV HRDLKPENLL YLTPEENSKI MITDFGLSKM EQNGVMSTAC 190 200 210 220 230 240 GTPGYVAPEV LAQKPYSKAV DCWSIGVITY ILLCGYPPFY EETESKLFEK IKEGYYEFES 250 260 270 280 290 300 PFWDDISESA KDFICHLLEK DPNERYTCEK ALRHPWIDGN TALHRDIYPS VSLQIQKNFA 310 320 330 340 350 360 KSKWRQAFNA AAVVHHMRKL HMNLHSPSVR QEVENRPPVS PAPEVSRPDS HDSSITEAPI 370 380 390 400 410 420 LDPSTPLPAL TRLPCSHSSR PSAPSGGRSL NCLVNGSLRI SSSLVPMQQG PLATGPCGCC 430 440 450 460 470 SSCLNIGNKG KSSYCSEPTL FRKANKKQNF KSEVMVPVKA GGSTHCRGGQ TGVCLVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of CLICK-III/CaMKIgamma, a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase (CaMK)." Takemoto-Kimura S., Terai H., Takamoto M., Ohmae S., Kikumura S., Segi E., Arakawa Y., Furuyashiki T., Narumiya S., Bito H. J. Biol. Chem. 278:18597-18605(2003) [PubMed: 12637513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, ENZYME REGULATION, MUTAGENESIS OF CYS-474, PHOSPHORYLATION BY CAMKK1, PHOSPHORYLATION OF CREB1, ISOPRENYLATION AT CYS-474, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	Bjork B.C., Watanabe Y., Murray J.C., Schutte B.C. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY212936 mRNA. Translation: AAP29965.1. AF428262 mRNA. Translation: AAL28101.1. BC021840 mRNA. Translation: AAH21840.1.
IPI	IPI00126681.
RefSeq	NP_659066.1. NM_144817.2.
UniGene	Mm.40329.
3D structure databases
ProteinModelPortal	Q91VB2.
SMR	Q91VB2. Positions 16-301.
ModBase	Search...
Protein-protein interaction databases
STRING	Q91VB2.
Proteomic databases
PRIDE	Q91VB2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000016323; ENSMUSP00000016323; ENSMUSG00000016179.
GeneID	215303.
KEGG	mmu:215303.
UCSC	uc007eel.1. mouse.
Organism-specific databases
CTD	57172.
MGI	MGI:2388073. Camk1g.
Phylogenomic databases
eggNOG	roNOG11129.
GeneTree	ENSGT00600000084207.
HOGENOM	HBG755340.
HOVERGEN	HBG108055.
InParanoid	Q91VB2.
OMA	MEQNGIM.
OrthoDB	EOG4Q58P6.
PhylomeDB	Q91VB2.
Enzyme and pathway databases
BRENDA	2.7.11.17. 3474.
Gene expression databases
ArrayExpress	Q91VB2.
Bgee	Q91VB2.
Genevestigator	Q91VB2.
GermOnline	ENSMUSG00000016179. Mus musculus.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08794.
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	374666.
SOURCE	Search...

Entry information

Entry name

KCC1G_MOUSE

Accession

Primary (citable) accession number: Q91VB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 1, 2001
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program