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Protein

Calcium/calmodulin-dependent protein kinase type 1G

Gene

Camk1g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by Ca2+/calmodulin. Binding of calmodulin is thought to result in a conformational change and leads to activation through phosphorylation by CAMKK1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATPPROSITE-ProRule annotation
Active sitei143 – 1431Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin-dependent protein kinase activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.17. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type 1G (EC:2.7.11.17)
Alternative name(s):
CaM kinase I gamma
Short name:
CaM kinase IG
Short name:
CaM-KI gamma
Short name:
CaMKI gamma
Short name:
CaMKIG
CaMK-like CREB kinase III
Short name:
CLICK III
Gene namesi
Name:Camk1g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2388073. Camk1g.

Subcellular locationi

GO - Cellular componenti

  • calcium- and calmodulin-dependent protein kinase complex Source: MGI
  • endomembrane system Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • neuron projection Source: Ensembl
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi474 – 4741C → S: Loss of association to membranes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Calcium/calmodulin-dependent protein kinase type 1GPRO_0000086085Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei121 – 1211PhosphoserineBy similarity
Modified residuei178 – 1781PhosphothreonineBy similarity

Post-translational modificationi

May be prenylated on Cys-474.1 Publication

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ91VB2.
PaxDbiQ91VB2.
PRIDEiQ91VB2.

PTM databases

iPTMnetiQ91VB2.
PhosphoSiteiQ91VB2.
SwissPalmiQ91VB2.

Expressioni

Tissue specificityi

Highly expressed in brain, in neuronal cell bodies of the central nucleus of amygdala and ventromedial hypothalamic nucleus. Also detected in heart, testis, and kidney.1 Publication

Developmental stagei

Expression starts at E11 in parallel with the onset of development of the central nervous system.1 Publication

Gene expression databases

BgeeiQ91VB2.
ExpressionAtlasiQ91VB2. baseline and differential.
GenevisibleiQ91VB2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000016323.

Structurei

3D structure databases

ProteinModelPortaliQ91VB2.
SMRiQ91VB2. Positions 15-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 277255Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni277 – 31741Autoinhibitory domainBy similarityAdd
BLAST
Regioni297 – 31822Calmodulin-bindingBy similarityAdd
BLAST

Domaini

The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ91VB2.
KOiK08794.
OMAiMVPVKTS.
PhylomeDBiQ91VB2.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91VB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKEEEDCS SWKKQTTNIR KTFIFMEVLG SGAFSEVFLV KQRVTGKLFA
60 70 80 90 100
LKCIKKSPAF RDSSLENEIA VLKRIKHENI VTLEDIYEST THYYLVMQLV
110 120 130 140 150
SGGELFDRIL ERGVYTEKDA SLVIQQVLSA VKYLHENGIV HRDLKPENLL
160 170 180 190 200
YLTPEENSKI MITDFGLSKM EQNGVMSTAC GTPGYVAPEV LAQKPYSKAV
210 220 230 240 250
DCWSIGVITY ILLCGYPPFY EETESKLFEK IKEGYYEFES PFWDDISESA
260 270 280 290 300
KDFICHLLEK DPNERYTCEK ALRHPWIDGN TALHRDIYPS VSLQIQKNFA
310 320 330 340 350
KSKWRQAFNA AAVVHHMRKL HMNLHSPSVR QEVENRPPVS PAPEVSRPDS
360 370 380 390 400
HDSSITEAPI LDPSTPLPAL TRLPCSHSSR PSAPSGGRSL NCLVNGSLRI
410 420 430 440 450
SSSLVPMQQG PLATGPCGCC SSCLNIGNKG KSSYCSEPTL FRKANKKQNF
460 470
KSEVMVPVKA GGSTHCRGGQ TGVCLVM
Length:477
Mass (Da):53,296
Last modified:December 1, 2001 - v1
Checksum:i3A61FBDC49A7BB56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY212936 mRNA. Translation: AAP29965.1.
AF428262 mRNA. Translation: AAL28101.1.
BC021840 mRNA. Translation: AAH21840.1.
CCDSiCCDS15638.1.
RefSeqiNP_659066.1. NM_144817.2.
UniGeneiMm.40329.

Genome annotation databases

EnsembliENSMUST00000016323; ENSMUSP00000016323; ENSMUSG00000016179.
GeneIDi215303.
KEGGimmu:215303.
UCSCiuc007eel.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY212936 mRNA. Translation: AAP29965.1.
AF428262 mRNA. Translation: AAL28101.1.
BC021840 mRNA. Translation: AAH21840.1.
CCDSiCCDS15638.1.
RefSeqiNP_659066.1. NM_144817.2.
UniGeneiMm.40329.

3D structure databases

ProteinModelPortaliQ91VB2.
SMRiQ91VB2. Positions 15-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000016323.

PTM databases

iPTMnetiQ91VB2.
PhosphoSiteiQ91VB2.
SwissPalmiQ91VB2.

Proteomic databases

MaxQBiQ91VB2.
PaxDbiQ91VB2.
PRIDEiQ91VB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016323; ENSMUSP00000016323; ENSMUSG00000016179.
GeneIDi215303.
KEGGimmu:215303.
UCSCiuc007eel.1. mouse.

Organism-specific databases

CTDi57172.
MGIiMGI:2388073. Camk1g.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ91VB2.
KOiK08794.
OMAiMVPVKTS.
PhylomeDBiQ91VB2.
TreeFamiTF314166.

Enzyme and pathway databases

BRENDAi2.7.11.17. 3474.

Miscellaneous databases

PROiQ91VB2.
SOURCEiSearch...

Gene expression databases

BgeeiQ91VB2.
ExpressionAtlasiQ91VB2. baseline and differential.
GenevisibleiQ91VB2. MM.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of CLICK-III/CaMKIgamma, a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase (CaMK)."
    Takemoto-Kimura S., Terai H., Takamoto M., Ohmae S., Kikumura S., Segi E., Arakawa Y., Furuyashiki T., Narumiya S., Bito H.
    J. Biol. Chem. 278:18597-18605(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, ENZYME REGULATION, MUTAGENESIS OF CYS-474, PHOSPHORYLATION BY CAMKK1, PHOSPHORYLATION OF CREB1, ISOPRENYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. Bjork B.C., Watanabe Y., Murray J.C., Schutte B.C.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiKCC1G_MOUSE
AccessioniPrimary (citable) accession number: Q91VB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.