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Protein

Calpain-8

Gene

Capn8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease (By similarity). Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex.By similarity1 Publication

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+CuratedNote: Binds 2 calcium ions.Curated

Enzyme regulationi

The concentration of calcium for half-maximal activity is 0.3 mM. Inhibited by calpastatin and calpeptin.1 Publication

pH dependencei

Optimum pH is 6.1 Publication

Temperature dependencei

Optimum temperature is 20 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105By similarity1
Active sitei262By similarity1
Active sitei286By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi588 – 5991Sequence analysisAdd BLAST12
Calcium bindingi618 – 6292Sequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B28. 3474.

Protein family/group databases

MEROPSiC02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-8 (EC:3.4.22.53)
Alternative name(s):
New calpain 2
Short name:
nCL-2
Stomach-specific M-type calpain
Gene namesi
Name:Capn8
Synonyms:Ncl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2181366. Capn8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003492811 – 703Calpain-8Add BLAST703

Post-translational modificationi

Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III.

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

EPDiQ91VA3.
PaxDbiQ91VA3.
PeptideAtlasiQ91VA3.
PRIDEiQ91VA3.

PTM databases

PhosphoSitePlusiQ91VA3.

Expressioni

Tissue specificityi

Predominantly expressed in the stomach. Localizes strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum.2 Publications

Gene expression databases

BgeeiENSMUSG00000038599.
CleanExiMM_CAPN8.
ExpressionAtlasiQ91VA3. baseline and differential.
GenevisibleiQ91VA3. MM.

Interactioni

Subunit structurei

Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047164.

Structurei

3D structure databases

ProteinModelPortaliQ91VA3.
SMRiQ91VA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 344Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini531 – 566EF-hand 1Add BLAST36
Domaini575 – 610EF-hand 2Add BLAST36
Domaini605 – 640EF-hand 3Add BLAST36
Domaini670 – 703EF-hand 4Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 512Domain IIIAdd BLAST158
Regioni513 – 531LinkerBy similarityAdd BLAST19
Regioni532 – 703Domain IVBy similarityAdd BLAST172

Domaini

The domain III mediates oligomerization.1 Publication

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ91VA3.
KOiK08577.
PhylomeDBiQ91VA3.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91VA3-1) [UniParc]FASTAAdd to basket
Also known as: nCL-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALAAGISK QRAAAQGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE
60 70 80 90 100
FPACPSALGY RDLGPGSAET QGIIWKRPTE LCSNPQFIVG GATRTDIRQG
110 120 130 140 150
GLGDCWLLAA IASLTLNEKL LYRVVPRDQS FQKNYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL LEKAYAKLNG SYEALAGGST
210 220 230 240 250
IEGFEDFTGG ISEFYDLRKP PGNLYYTIQK ALRKGSLLGC SIDVSNAAEA
260 270 280 290 300
EATTRQKLVK GHAYSVTGVE EVDFRGLPEK LIRLRNPWGE VEWTGAWSDS
310 320 330 340 350
APEWNYIDPQ KKGELDKRAE DGEFWMSFSD FLKQFSRLEI CNLSPDSLSS
360 370 380 390 400
EEIHKWNLVL FNGRWTRGST AGGCQNYPAT YWTNPQFKIH LDEVDEDQEE
410 420 430 440 450
GTSEPCCTVL LGLMQKNRRR QRRIGQGMLS IGYAVYQIPK ELENHTDEHL
460 470 480 490 500
GRDFFQGRQP STCSSTYMNL REVSSRVQLP PGQYLVVPST FEPFKDGDFC
510 520 530 540 550
LRVFSEKKAQ ALEIGDAVPG DPHEPHPRDM DGEDEHFWSL SEEFADKDSE
560 570 580 590 600
ISAHQLKRVL NGLLSKRTDM KFDGFNINTC REMISLLDGD GTGSLRPVEF
610 620 630 640 650
KTLWLKICKY LEIYQEMDHS RAGTIDAHEM RTALKKAGFT LNNQVQQTIA
660 670 680 690 700
TRYACSKLGV DFDGFVACMI RLEILFKLFR LLDKDQNGIV QLSLAEWLCR

ALV
Length:703
Mass (Da):79,335
Last modified:December 1, 2001 - v1
Checksum:i50F2F9CCF2192FB6
GO
Isoform 2 (identifier: Q91VA3-2) [UniParc]FASTAAdd to basket
Also known as: Calpain 8b, nCL-2'

The sequence of this isoform differs from the canonical sequence as follows:
     379-381: ATY → GSF
     382-703: Missing.

Show »
Length:381
Mass (Da):42,434
Checksum:iECCF74CE9840256F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti354H → Q in BAB70479 (PubMed:11523006).Curated1
Sequence conflicti519P → A in AAI25505 (PubMed:15489334).Curated1
Sequence conflicti519P → A in AAI25509 (PubMed:15489334).Curated1
Sequence conflicti700R → C in AAI25505 (PubMed:15489334).Curated1
Sequence conflicti700R → C in AAI25509 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035306379 – 381ATY → GSF in isoform 2. 1 Publication3
Alternative sequenceiVSP_035307382 – 703Missing in isoform 2. 1 PublicationAdd BLAST322

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050201 Genomic DNA. Translation: BAB70479.1.
AB050202 mRNA. Translation: BAB70480.1.
AB050203 mRNA. Translation: BAB70481.1.
AB061518 mRNA. Translation: BAB55000.1.
AB061519 mRNA. Translation: BAB55001.1.
BC125504 mRNA. Translation: AAI25505.1.
BC125508 mRNA. Translation: AAI25509.1.
CCDSiCCDS35814.1. [Q91VA3-1]
CCDS48477.1. [Q91VA3-2]
RefSeqiNP_001139278.1. NM_001145806.1. [Q91VA3-2]
NP_570960.2. NM_130890.2.
UniGeneiMm.189201.

Genome annotation databases

EnsembliENSMUST00000168514; ENSMUSP00000129549; ENSMUSG00000038599. [Q91VA3-2]
GeneIDi170725.
KEGGimmu:170725.
UCSCiuc007dyh.2. mouse. [Q91VA3-2]
uc007dyi.2. mouse. [Q91VA3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050201 Genomic DNA. Translation: BAB70479.1.
AB050202 mRNA. Translation: BAB70480.1.
AB050203 mRNA. Translation: BAB70481.1.
AB061518 mRNA. Translation: BAB55000.1.
AB061519 mRNA. Translation: BAB55001.1.
BC125504 mRNA. Translation: AAI25505.1.
BC125508 mRNA. Translation: AAI25509.1.
CCDSiCCDS35814.1. [Q91VA3-1]
CCDS48477.1. [Q91VA3-2]
RefSeqiNP_001139278.1. NM_001145806.1. [Q91VA3-2]
NP_570960.2. NM_130890.2.
UniGeneiMm.189201.

3D structure databases

ProteinModelPortaliQ91VA3.
SMRiQ91VA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047164.

Protein family/group databases

MEROPSiC02.007.

PTM databases

PhosphoSitePlusiQ91VA3.

Proteomic databases

EPDiQ91VA3.
PaxDbiQ91VA3.
PeptideAtlasiQ91VA3.
PRIDEiQ91VA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000168514; ENSMUSP00000129549; ENSMUSG00000038599. [Q91VA3-2]
GeneIDi170725.
KEGGimmu:170725.
UCSCiuc007dyh.2. mouse. [Q91VA3-2]
uc007dyi.2. mouse. [Q91VA3-1]

Organism-specific databases

CTDi388743.
MGIiMGI:2181366. Capn8.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ91VA3.
KOiK08577.
PhylomeDBiQ91VA3.
TreeFamiTF314748.

Enzyme and pathway databases

BRENDAi3.4.22.B28. 3474.

Miscellaneous databases

PROiQ91VA3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038599.
CleanExiMM_CAPN8.
ExpressionAtlasiQ91VA3. baseline and differential.
GenevisibleiQ91VA3. MM.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN8_MOUSE
AccessioniPrimary (citable) accession number: Q91VA3
Secondary accession number(s): Q059W1, Q91UZ9, Q920R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.