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Protein

Acyl-coenzyme A synthetase ACSM1, mitochondrial

Gene

Acsm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase (By similarity). Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro).By similarity1 Publication

Catalytic activityi

ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.
GTP + lipoate = diphosphate + lipoyl-GMP.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei448ATPBy similarity1
Binding sitei463ATPBy similarity1
Binding sitei559ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi222 – 230ATPBy similarity9

GO - Molecular functioni

  • acyl-CoA ligase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • butyrate-CoA ligase activity Source: UniProtKB
  • fatty acid ligase activity Source: MGI
  • fatty-acyl-CoA synthase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • acyl-CoA metabolic process Source: GO_Central
  • fatty acid biosynthetic process Source: MGI

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-177135 Conjugation of benzoate with glycine
R-MMU-177162 Conjugation of phenylacetate with glutamine

Chemistry databases

SwissLipidsiSLP:000001205

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A synthetase ACSM1, mitochondrial (EC:6.2.1.2)
Alternative name(s):
Acyl-CoA synthetase medium-chain family member 1
Butyrate--CoA ligase 1
Butyryl-coenzyme A synthetase 1
Lipoate-activating enzyme
Middle-chain acyl-CoA synthetase 1
Gene namesi
Name:Acsm1
Synonyms:Bucs1, Lae, Macs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2152200 Acsm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionSequence analysisAdd BLAST35
ChainiPRO_000030609236 – 573Acyl-coenzyme A synthetase ACSM1, mitochondrialAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81N6-succinyllysineCombined sources1
Modified residuei142N6-acetyllysine; alternateCombined sources1
Modified residuei142N6-succinyllysine; alternateCombined sources1
Modified residuei179N6-succinyllysineCombined sources1
Modified residuei200N6-acetyllysine; alternateCombined sources1
Modified residuei200N6-succinyllysine; alternateCombined sources1
Modified residuei210N6-acetyllysineCombined sources1
Modified residuei233N6-succinyllysineCombined sources1
Modified residuei324N6-succinyllysineCombined sources1
Modified residuei352N6-acetyllysine; alternateCombined sources1
Modified residuei352N6-succinyllysine; alternateCombined sources1
Modified residuei387N6-acetyllysine; alternateCombined sources1
Modified residuei387N6-succinyllysine; alternateCombined sources1
Modified residuei501N6-succinyllysineCombined sources1
Modified residuei527N6-acetyllysineCombined sources1
Modified residuei534N6-acetyllysine; alternateCombined sources1
Modified residuei534N6-succinyllysine; alternateCombined sources1
Modified residuei545N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ91VA0
PeptideAtlasiQ91VA0
PRIDEiQ91VA0

PTM databases

iPTMnetiQ91VA0
PhosphoSitePlusiQ91VA0
SwissPalmiQ91VA0

Expressioni

Tissue specificityi

Highly expressed in liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000033533
CleanExiMM_ACSM1
ExpressionAtlasiQ91VA0 baseline and differential
GenevisibleiQ91VA0 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ91VA0, 3 interactors
MINTiQ91VA0
STRINGi10090.ENSMUSP00000036140

Structurei

3D structure databases

ProteinModelPortaliQ91VA0
SMRiQ91VA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1175 Eukaryota
COG0365 LUCA
GeneTreeiENSGT00760000119178
HOGENOMiHOG000229982
HOVERGENiHBG053031
InParanoidiQ91VA0
KOiK01896
OMAiEEWKRRT
OrthoDBiEOG091G03NW
PhylomeDBiQ91VA0
TreeFamiTF354287

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91VA0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQWLKSFQIC KVLQGFSLSP TQLHRRLFSR VGAPRWNDHD SPEEFNFASD
60 70 80 90 100
VLDYWAQMEE EGKRGPSPAF WWVNGQGDEI KWSFRKLRDL TCRTANVFEQ
110 120 130 140 150
ICGLQQGDHL ALILPRVPEW WLVTVGCMRT GIIFMPGTTQ LKAKDILYRI
160 170 180 190 200
QISRAKAIVT TASLVPEVES VASECPDLKT KLVVSDHSHE GWLDFCSLIK
210 220 230 240 250
SASPDHTCIK SKMKDPMAIF FTSGTTGYPK MAKHNQGLAF RSYIPSCRKL
260 270 280 290 300
LKLKTSDILW CMSDPGWILA TVGCLIEPWT SGCTVFIHHL PQFDPKVIVE
310 320 330 340 350
VLFKYPITQC LAAPGVYRMV LQQKTSNLRF PTLEHCTTGG ESLLPEEYEQ
360 370 380 390 400
WKQRTGLSIH EVYGQSETGI SSATLREMKI KRGSIGKAIL PFDLQIIDEK
410 420 430 440 450
GNILPPNTEG YIGIRIKPTR PLGLFMEYEN SPESTSEVEC GDFYNSGDRA
460 470 480 490 500
TIDEEGYIWF LGRGDDVINA SGYRIGPVEV ENALAEHPAV AESAVVSSPD
510 520 530 540 550
KDRGEVVKAF IVLNPEFLSH DQEQLIKELQ HHVKSVTAPY KYPRKVEFVS
560 570
ELPKTVTGKI KRKELRNKEF GQL
Length:573
Mass (Da):64,760
Last modified:December 1, 2001 - v1
Checksum:i557F25D467CA305B
GO
Isoform 2 (identifier: Q91VA0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     369-395: Missing.

Note: No experimental confirmation available.
Show »
Length:546
Mass (Da):61,848
Checksum:iDAAF1F2C5EADB8F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti465D → V in BAE28977 (PubMed:16141072).Curated1
Sequence conflicti525L → F in BAE28977 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028392369 – 395Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059428 mRNA Translation: BAB64534.1
AK149550 mRNA Translation: BAE28952.1
AK149586 mRNA Translation: BAE28977.1
BC016414 mRNA Translation: AAH16414.1
BC022149 mRNA Translation: AAH22149.1
CCDSiCCDS21783.1 [Q91VA0-1]
RefSeqiNP_473435.1, NM_054094.5 [Q91VA0-1]
UniGeneiMm.427560

Genome annotation databases

EnsembliENSMUST00000047929; ENSMUSP00000036140; ENSMUSG00000033533 [Q91VA0-1]
GeneIDi117147
KEGGimmu:117147
UCSCiuc009jlm.1 mouse [Q91VA0-1]
uc012fsv.1 mouse [Q91VA0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACSM1_MOUSE
AccessioniPrimary (citable) accession number: Q91VA0
Secondary accession number(s): Q3UED4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health