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Protein

Endosialin

Gene

Cd248

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in angiogenesis or vascular function.1 Publication

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • carbohydrate binding Source: UniProtKB-KW
  • extracellular matrix binding Source: MGI

GO - Biological processi

  • anatomical structure regression Source: MGI
  • cell migration Source: MGI
  • lymph node development Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of endothelial cell apoptotic process Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Endosialin
Alternative name(s):
Tumor endothelial marker 1
CD_antigen: CD248
Gene namesi
Name:Cd248
Synonyms:Tem1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1917695. Cd248.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 695ExtracellularSequence analysisAdd BLAST678
Transmembranei696 – 716HelicalSequence analysisAdd BLAST21
Topological domaini717 – 765CytoplasmicSequence analysisAdd BLAST49

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000004580018 – 765EndosialinAdd BLAST748

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi131 ↔ 147PROSITE-ProRule annotation
Disulfide bondi164 ↔ 213PROSITE-ProRule annotation
Disulfide bondi203 ↔ 230PROSITE-ProRule annotation
Disulfide bondi316 ↔ 326PROSITE-ProRule annotation
Disulfide bondi322 ↔ 335PROSITE-ProRule annotation
Disulfide bondi337 ↔ 350PROSITE-ProRule annotation
Glycosylationi401O-linked (GalNAc...)Sequence analysis1
Glycosylationi428O-linked (GalNAc...)Sequence analysis1
Glycosylationi448O-linked (GalNAc...)Sequence analysis1
Glycosylationi456O-linked (GalNAc...)Sequence analysis1
Glycosylationi459O-linked (GalNAc...)Sequence analysis1
Glycosylationi466O-linked (GalNAc...)Sequence analysis1
Glycosylationi467O-linked (GalNAc...)Sequence analysis1
Glycosylationi470O-linked (GalNAc...)Sequence analysis1
Glycosylationi472O-linked (GalNAc...)Sequence analysis1
Glycosylationi477O-linked (GalNAc...)Sequence analysis1
Glycosylationi488O-linked (GalNAc...)Sequence analysis1
Glycosylationi517O-linked (GalNAc...)Sequence analysis1
Glycosylationi520O-linked (GalNAc...)Sequence analysis1
Glycosylationi535O-linked (GalNAc...)Sequence analysis1
Glycosylationi552O-linked (GalNAc...)Sequence analysis1
Glycosylationi554O-linked (GalNAc...)Sequence analysis1
Glycosylationi556O-linked (GalNAc...)Sequence analysis1
Glycosylationi570O-linked (GalNAc...)Sequence analysis1
Glycosylationi571O-linked (GalNAc...)Sequence analysis1
Glycosylationi604O-linked (GalNAc...)Sequence analysis1
Glycosylationi613O-linked (GalNAc...)Sequence analysis1
Glycosylationi626O-linked (GalNAc...)Sequence analysis1
Glycosylationi627O-linked (GalNAc...)Sequence analysis1
Glycosylationi635O-linked (GalNAc...)Sequence analysis1
Glycosylationi638O-linked (GalNAc...)Sequence analysis1
Glycosylationi639O-linked (GalNAc...)Sequence analysis1
Glycosylationi640O-linked (GalNAc...)Sequence analysis1
Glycosylationi644O-linked (GalNAc...)Sequence analysis1
Glycosylationi663O-linked (GalNAc...)Sequence analysis1
Glycosylationi673O-linked (GalNAc...)Sequence analysis1
Modified residuei754PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated by sialylated oligosaccharides.By similarity
May be N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ91V98.
PaxDbiQ91V98.
PeptideAtlasiQ91V98.
PRIDEiQ91V98.

PTM databases

iPTMnetiQ91V98.
PhosphoSitePlusiQ91V98.

Expressioni

Tissue specificityi

Expressed in cell lines derived from endothelial cells, embryonic fibroblasts and preadipocytes.1 Publication

Developmental stagei

Detected at E19 in embryo.1 Publication

Gene expression databases

BgeeiENSMUSG00000056481.
CleanExiMM_CD248.
GenevisibleiQ91V98. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070847.

Structurei

3D structure databases

ProteinModelPortaliQ91V98.
SMRiQ91V98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 156C-type lectinPROSITE-ProRule annotationAdd BLAST127
Domaini162 – 232SushiAdd BLAST71
Domaini312 – 351EGF-like; calcium-bindingSequence analysisAdd BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi400 – 676Pro-richAdd BLAST277

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 Sushi (CCP/SCR) domain.Curated

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJQN. Eukaryota.
ENOG410ZKXP. LUCA.
GeneTreeiENSGT00810000125382.
HOGENOMiHOG000111482.
HOVERGENiHBG081065.
InParanoidiQ91V98.
KOiK06706.
OMAiEASGEHR.
OrthoDBiEOG091G035H.
PhylomeDBiQ91V98.
TreeFamiTF330714.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
SM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91V98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRLLLAWV AAVPALGQVP WTPEPRAACG PSSCYALFPR RRTFLEAWRA
60 70 80 90 100
CRELGGNLAT PRTPEEAQRV DSLVGVGPAN GLLWIGLQRQ ARQCQPQRPL
110 120 130 140 150
RGFIWTTGDQ DTAFTNWAQP ATEGPCPAQR CAALEASGEH RWLEGSCTLA
160 170 180 190 200
VDGYLCQFGF EGACPALPLE VGQAGPAVYT TPFNLVSSEF EWLPFGSVAA
210 220 230 240 250
VQCQAGRGAS LLCVKQPSGG VGWSQTGPLC PGTGCGPDNG GCEHECVEEV
260 270 280 290 300
DGAVSCRCSE GFRLAADGHS CEDPCAQAPC EQQCEPGGPQ GYSCHCRLGF
310 320 330 340 350
RPAEDDPHRC VDTDECQIAG VCQQMCVNYV GGFECYCSEG HELEADGISC
360 370 380 390 400
SPAGAMGAQA SQDLRDELLD DGEEGEDEEE PWEDFDGTWT EEQGILWLAP
410 420 430 440 450
THPPDFGLPY RPNFPQDGEP QRLHLEPTWP PPLSAPRGPY HSSVVSATRP
460 470 480 490 500
MVISATRPTL PSAHKTSVIS ATRPPLSPVH PPAMAPATPP AVFSEHQIPK
510 520 530 540 550
IKANYPDLPF GHKPGITSAT HPARSPPYQP PIISTNYPQV FPPHQAPMSP
560 570 580 590 600
DTHTITYLPP VPPHLDPGDT TSKAHQHPLL PDAPGIRTQA PQLSVSALQP
610 620 630 640 650
PLPTNSRSSV HETPVPAANQ PPAFPSSPLP PQRPTNQTSS ISPTHSYSRA
660 670 680 690 700
PLVPREGVPS PKSVPQLPSV PSTAAPTALA ESGLAGQSQR DDRWLLVALL
710 720 730 740 750
VPTCVFLVVL LALGIVYCTR CGSHAPNKRI TDCYRWVTHA GNKSSTEPMP
760
PRGSLTGVQT CRTSV
Length:765
Mass (Da):81,813
Last modified:December 1, 2001 - v1
Checksum:i572A06CC15BC8CD8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti525S → P in AAK84664 (PubMed:11489895).Curated1
Sequence conflicti751P → T in BAE25992 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378758 mRNA. Translation: AAL11995.1.
AF388572 mRNA. Translation: AAK84664.1.
AF388573 Genomic DNA. Translation: AAK84665.1.
AK144655 mRNA. Translation: BAE25992.1.
BC046318 mRNA. Translation: AAH46318.1.
CCDSiCCDS29448.1.
RefSeqiNP_473383.1. NM_054042.2.
UniGeneiMm.29597.

Genome annotation databases

EnsembliENSMUST00000070630; ENSMUSP00000070847; ENSMUSG00000056481.
GeneIDi70445.
KEGGimmu:70445.
UCSCiuc008gcb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378758 mRNA. Translation: AAL11995.1.
AF388572 mRNA. Translation: AAK84664.1.
AF388573 Genomic DNA. Translation: AAK84665.1.
AK144655 mRNA. Translation: BAE25992.1.
BC046318 mRNA. Translation: AAH46318.1.
CCDSiCCDS29448.1.
RefSeqiNP_473383.1. NM_054042.2.
UniGeneiMm.29597.

3D structure databases

ProteinModelPortaliQ91V98.
SMRiQ91V98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070847.

PTM databases

iPTMnetiQ91V98.
PhosphoSitePlusiQ91V98.

Proteomic databases

MaxQBiQ91V98.
PaxDbiQ91V98.
PeptideAtlasiQ91V98.
PRIDEiQ91V98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070630; ENSMUSP00000070847; ENSMUSG00000056481.
GeneIDi70445.
KEGGimmu:70445.
UCSCiuc008gcb.1. mouse.

Organism-specific databases

CTDi57124.
MGIiMGI:1917695. Cd248.

Phylogenomic databases

eggNOGiENOG410IJQN. Eukaryota.
ENOG410ZKXP. LUCA.
GeneTreeiENSGT00810000125382.
HOGENOMiHOG000111482.
HOVERGENiHBG081065.
InParanoidiQ91V98.
KOiK06706.
OMAiEASGEHR.
OrthoDBiEOG091G035H.
PhylomeDBiQ91V98.
TreeFamiTF330714.

Miscellaneous databases

ChiTaRSiCd248. mouse.
PROiQ91V98.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056481.
CleanExiMM_CD248.
GenevisibleiQ91V98. MM.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
SM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD248_MOUSE
AccessioniPrimary (citable) accession number: Q91V98
Secondary accession number(s): Q3UMV6, Q91ZV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.