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Protein

Nephronectin

Gene

Npnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.3 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • integrin binding Source: BHF-UCL

GO - Biological processi

  • branching involved in ureteric bud morphogenesis Source: MGI
  • cell-cell adhesion mediated by integrin Source: BHF-UCL
  • cell-matrix adhesion Source: MGI
  • cellular response to tumor necrosis factor Source: BHF-UCL
  • establishment of protein localization Source: BHF-UCL
  • extracellular matrix organization Source: MGI
  • pilomotor reflex Source: BHF-UCL
  • positive regulation of alkaline phosphatase activity Source: BHF-UCL
  • positive regulation of cell-substrate adhesion Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  • positive regulation of osteoblast differentiation Source: BHF-UCL
  • positive regulation of smooth muscle contraction Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation Source: BHF-UCL
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • ureteric bud development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell adhesion, Differentiation

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Nephronectin
Alternative name(s):
Preosteoblast EGF-like repeat protein with MAM domain
Gene namesi
Name:Npnt
Synonyms:Neph1, Poem
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2148811. Npnt.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: BHF-UCL
  • extracellular exosome Source: MGI
  • extracellular matrix Source: BHF-UCL
  • extracellular region Source: BHF-UCL
  • membrane Source: InterPro
  • proteinaceous extracellular matrix Source: MGI
  • smooth muscle contractile fiber Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice display renal agenesis at birth due to a developmental delay. This delay is associated with a reduced expression of Gdnf and is similar to the one found in mice lacking Itga8.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi384D → E: Reduced cell spreading- and survival-promoting activities. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000029568520 – 561NephronectinAdd BLAST542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 69PROSITE-ProRule annotation
Disulfide bondi60 ↔ 75PROSITE-ProRule annotation
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi93 ↔ 104PROSITE-ProRule annotation
Disulfide bondi100 ↔ 113PROSITE-ProRule annotation
Disulfide bondi115 ↔ 127PROSITE-ProRule annotation
Disulfide bondi173 ↔ 186PROSITE-ProRule annotation
Disulfide bondi180 ↔ 195PROSITE-ProRule annotation
Disulfide bondi197 ↔ 212PROSITE-ProRule annotation
Disulfide bondi218 ↔ 231PROSITE-ProRule annotation
Disulfide bondi225 ↔ 240PROSITE-ProRule annotation
Disulfide bondi242 ↔ 253PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ91V88.
PRIDEiQ91V88.

PTM databases

iPTMnetiQ91V88.
PhosphoSitePlusiQ91V88.

Expressioni

Tissue specificityi

Expressed in kidney (at protein level).1 Publication

Developmental stagei

Expressed from 10.5 dpc onward mainly at epithelial-mesenchymal interfaces in kidney and other tissues undergoing morphogenesis (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000040998.
CleanExiMM_NPNT.
ExpressionAtlasiQ91V88. baseline and differential.
GenevisibleiQ91V88. MM.

Interactioni

Subunit structurei

Homodimer and homotrimer.

GO - Molecular functioni

  • integrin binding Source: BHF-UCL

Protein-protein interaction databases

IntActiQ91V88. 2 interactors.
STRINGi10090.ENSMUSP00000040071.

Structurei

3D structure databases

ProteinModelPortaliQ91V88.
SMRiQ91V88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 87EGF-like 1PROSITE-ProRule annotationAdd BLAST36
Domaini89 – 128EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST40
Domaini132 – 168EGF-like 3PROSITE-ProRule annotationAdd BLAST37
Domaini169 – 213EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST45
Domaini214 – 254EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini420 – 561MAMPROSITE-ProRule annotationAdd BLAST142

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi382 – 384Integrin interaction3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi296 – 359Pro-richAdd BLAST64

Domaini

The MAM domain is required for localization at the cell surface.

Sequence similaritiesi

Belongs to the nephronectin family.Curated
Contains 5 EGF-like domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IR6Z. Eukaryota.
ENOG411039V. LUCA.
GeneTreeiENSGT00860000133741.
HOGENOMiHOG000059638.
HOVERGENiHBG108200.
InParanoidiQ91V88.
KOiK06824.
OMAiSLKKGHC.
OrthoDBiEOG091G02IF.
PhylomeDBiQ91V88.
TreeFamiTF330819.

Family and domain databases

CDDicd06263. MAM. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR000998. MAM_dom.
[Graphical view]
PfamiPF07645. EGF_CA. 3 hits.
PF00629. MAM. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 3 hits.
SM00137. MAM. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 3 hits.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 3 hits.
PS50060. MAM_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91V88-1) [UniParc]FASTAAdd to basket
Also known as: Short

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVLLAAVLA SSLYLQVAAD FDGRWPRQIV SSIGLCRYGG RIDCCWGWAR
60 70 80 90 100
QSWGQCQPVC QPQCKHGECV GPNKCKCHPG FAGKTCNQDL NECGLKPRPC
110 120 130 140 150
KHRCMNTFGS YKCYCLNGYM LLPDGSCSSA LSCSMANCQY GCDVVKGQVR
160 170 180 190 200
CQCPSPGLQL APDGRTCVDI DECATGRVSC PRFRQCVNTF GSYICKCHTG
210 220 230 240 250
FDLMYIGGKY QCHDIDECSL GQHQCSSYAR CYNIHGSYKC QCRDGYEGDG
260 270 280 290 300
LNCVYIPKVM IEPSGPIHMP ERNGTISKGD GGHANRIPDA GSTRWPLKTP
310 320 330 340 350
YIPPVITNRP TSKPTTRPTP NPTPQPTPPP PPPLPTEPRT TPLPPTPERP
360 370 380 390 400
STRPTTIAPA TSTTTRVITV DNRIQTDPQK PRGDVFIPRQ PTNDLFEIFE
410 420 430 440 450
IERGVSADEE VKDDPGILIH SCNFDHGLCG WIREKDSDLH WETARDPAGG
460 470 480 490 500
QYLTVSAAKA PGGKAARLVL RLGHLMHSGD LCLSFRHKVT GLHSGTLQVF
510 520 530 540 550
VRKHGTHGAA LWGRNGGHGW RQTQITLRGA DVKSVIFKGE KRRGHTGEIG
560
LDDVSLKRGR C
Length:561
Mass (Da):61,490
Last modified:December 1, 2001 - v1
Checksum:i69E7ACAA0EE3F506
GO
Isoform 2 (identifier: Q91V88-2) [UniParc]FASTAAdd to basket
Also known as: Long

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: P → PFYVLRQRLARIRCQLKA

Show »
Length:578
Mass (Da):63,607
Checksum:i4757DE54CC47DA4C
GO
Isoform 3 (identifier: Q91V88-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-88: Q → QDESFHPTPLDQGSEQPLFQPPDHQATNVPSR

Show »
Length:592
Mass (Da):64,945
Checksum:i21943B9077691396
GO
Isoform 4 (identifier: Q91V88-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: P → PFYVLRQRLARIRCQLKA
     88-88: Q → QDESFHPTPLDQGSEQPLFQPPDHQATNVPSR

Show »
Length:609
Mass (Da):67,061
Checksum:i44C45DE3EBEC4BDC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24R → G in AAH68308 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02698958P → PFYVLRQRLARIRCQLKA in isoform 2 and isoform 4. 1 Publication1
Alternative sequenceiVSP_02699088Q → QDESFHPTPLDQGSEQPLFQ PPDHQATNVPSR in isoform 3 and isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059656 mRNA. Translation: BAB69692.1.
AF397007 mRNA. Translation: AAK84391.1.
AF397008 mRNA. Translation: AAK84392.1.
AY035898 mRNA. Translation: AAK96010.1.
AY035899 mRNA. Translation: AAK96011.1.
AK050484 mRNA. Translation: BAC34283.1.
BC046642 mRNA. Translation: AAH46642.1.
BC068308 mRNA. Translation: AAH68308.1.
CCDSiCCDS17845.1. [Q91V88-1]
CCDS17846.1. [Q91V88-2]
CCDS71322.1. [Q91V88-3]
CCDS71323.1. [Q91V88-4]
RefSeqiNP_001025007.1. NM_001029836.2. [Q91V88-1]
NP_001274030.1. NM_001287101.1. [Q91V88-4]
NP_001274031.1. NM_001287102.1. [Q91V88-3]
NP_277060.2. NM_033525.3. [Q91V88-2]
UniGeneiMm.279310.
Mm.440226.

Genome annotation databases

EnsembliENSMUST00000042729; ENSMUSP00000040071; ENSMUSG00000040998. [Q91V88-2]
ENSMUST00000042744; ENSMUSP00000040684; ENSMUSG00000040998. [Q91V88-1]
ENSMUST00000093971; ENSMUSP00000091505; ENSMUSG00000040998. [Q91V88-4]
ENSMUST00000117164; ENSMUSP00000113419; ENSMUSG00000040998. [Q91V88-3]
GeneIDi114249.
KEGGimmu:114249.
UCSCiuc008rkc.3. mouse. [Q91V88-2]
uc008rkd.3. mouse. [Q91V88-1]
uc008rke.3. mouse. [Q91V88-4]
uc008rkf.3. mouse. [Q91V88-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059656 mRNA. Translation: BAB69692.1.
AF397007 mRNA. Translation: AAK84391.1.
AF397008 mRNA. Translation: AAK84392.1.
AY035898 mRNA. Translation: AAK96010.1.
AY035899 mRNA. Translation: AAK96011.1.
AK050484 mRNA. Translation: BAC34283.1.
BC046642 mRNA. Translation: AAH46642.1.
BC068308 mRNA. Translation: AAH68308.1.
CCDSiCCDS17845.1. [Q91V88-1]
CCDS17846.1. [Q91V88-2]
CCDS71322.1. [Q91V88-3]
CCDS71323.1. [Q91V88-4]
RefSeqiNP_001025007.1. NM_001029836.2. [Q91V88-1]
NP_001274030.1. NM_001287101.1. [Q91V88-4]
NP_001274031.1. NM_001287102.1. [Q91V88-3]
NP_277060.2. NM_033525.3. [Q91V88-2]
UniGeneiMm.279310.
Mm.440226.

3D structure databases

ProteinModelPortaliQ91V88.
SMRiQ91V88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91V88. 2 interactors.
STRINGi10090.ENSMUSP00000040071.

PTM databases

iPTMnetiQ91V88.
PhosphoSitePlusiQ91V88.

Proteomic databases

PaxDbiQ91V88.
PRIDEiQ91V88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042729; ENSMUSP00000040071; ENSMUSG00000040998. [Q91V88-2]
ENSMUST00000042744; ENSMUSP00000040684; ENSMUSG00000040998. [Q91V88-1]
ENSMUST00000093971; ENSMUSP00000091505; ENSMUSG00000040998. [Q91V88-4]
ENSMUST00000117164; ENSMUSP00000113419; ENSMUSG00000040998. [Q91V88-3]
GeneIDi114249.
KEGGimmu:114249.
UCSCiuc008rkc.3. mouse. [Q91V88-2]
uc008rkd.3. mouse. [Q91V88-1]
uc008rke.3. mouse. [Q91V88-4]
uc008rkf.3. mouse. [Q91V88-3]

Organism-specific databases

CTDi255743.
MGIiMGI:2148811. Npnt.

Phylogenomic databases

eggNOGiENOG410IR6Z. Eukaryota.
ENOG411039V. LUCA.
GeneTreeiENSGT00860000133741.
HOGENOMiHOG000059638.
HOVERGENiHBG108200.
InParanoidiQ91V88.
KOiK06824.
OMAiSLKKGHC.
OrthoDBiEOG091G02IF.
PhylomeDBiQ91V88.
TreeFamiTF330819.

Miscellaneous databases

PROiQ91V88.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040998.
CleanExiMM_NPNT.
ExpressionAtlasiQ91V88. baseline and differential.
GenevisibleiQ91V88. MM.

Family and domain databases

CDDicd06263. MAM. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR000998. MAM_dom.
[Graphical view]
PfamiPF07645. EGF_CA. 3 hits.
PF00629. MAM. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 3 hits.
SM00137. MAM. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 3 hits.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 3 hits.
PS50060. MAM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPNT_MOUSE
AccessioniPrimary (citable) accession number: Q91V88
Secondary accession number(s): Q6NV58
, Q80VP6, Q91XL5, Q91ZD3, Q923T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Was named nephronectin based on its potential role in kidney development.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.