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Protein

N-chimaerin

Gene

Chn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein for p21-rac and a phorbol ester receptor. May play an important role in neuronal signal-transduction mechanisms (By similarity). Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri205 – 255Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • ephrin receptor binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ephrin receptor signaling pathway Source: UniProtKB
  • intracellular signal transduction Source: InterPro
  • motor neuron axon guidance Source: UniProtKB
  • regulation of axonogenesis Source: UniProtKB
  • regulation of GTPase activity Source: UniProtKB
  • regulation of small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionGTPase activation
Biological processNeurogenesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
N-chimaerin
Alternative name(s):
A-chimaerin
Alpha-chimerin
N-chimerin
Short name:
NC
Rho GTPase-activating protein 2
Gene namesi
Name:Chn1
Synonyms:Arhgap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1915674 Chn1

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile but display a loss of coordination of limb movement which phenocopies the one of Epha4 mutant mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000566952 – 459N-chimaerinAdd BLAST458

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei192PhosphothreonineBy similarity1
Modified residuei340PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is EPHA4 kinase activity-dependent.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ91V57
PRIDEiQ91V57

PTM databases

iPTMnetiQ91V57
PhosphoSitePlusiQ91V57

Expressioni

Gene expression databases

BgeeiENSMUSG00000056486
CleanExiMM_CHN1
ExpressionAtlasiQ91V57 baseline and differential
GenevisibleiQ91V57 MM

Interactioni

Subunit structurei

Interacts with EPHA4; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation. May also interact with EPHB1 and EPHB2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Epha4Q031372EBI-1539203,EBI-1539152

GO - Molecular functioni

  • ephrin receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi224378, 3 interactors
IntActiQ91V57, 1 interactor
MINTiQ91V57
STRINGi10090.ENSMUSP00000107655

Structurei

3D structure databases

ProteinModelPortaliQ91V57
SMRiQ91V57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 135SH2PROSITE-ProRule annotationAdd BLAST87
Domaini268 – 459Rho-GAPPROSITE-ProRule annotationAdd BLAST192

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri205 – 255Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

SH2 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG1453 Eukaryota
ENOG410YM3I LUCA
GeneTreeiENSGT00890000139322
HOGENOMiHOG000231926
HOVERGENiHBG080489
InParanoidiQ91V57
KOiK20630
OMAiREPAYQK
OrthoDBiEOG091G0834
PhylomeDBiQ91V57
TreeFamiTF342052

Family and domain databases

CDDicd00029 C1, 1 hit
cd04372 RhoGAP_chimaerin, 1 hit
cd10352 SH2_a2chimerin_b2chimerin, 1 hit
Gene3Di1.10.555.10, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR035840 Chimaerin_SH2
IPR017356 CHN1/CHN2
IPR020454 DAG/PE-bd
IPR002219 PE/DAG-bd
IPR008936 Rho_GTPase_activation_prot
IPR037860 RhoGAP_chimaerin
IPR000198 RhoGAP_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00620 RhoGAP, 1 hit
PF00017 SH2, 1 hit
PIRSFiPIRSF038015 N-chimaerin, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00324 RhoGAP, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50238 RHOGAP, 1 hit
PS50001 SH2, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91V57-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALTLFDTDE YRPPVWKSYL YQLQQEAPHP RRVTCTCEVE NRPKYYGREY
60 70 80 90 100
HGMISREETD QLLSVAEGSY LIRESQRQPG TYTLALRFGS QTRNFRLYYD
110 120 130 140 150
GKHFVGEKRF ESIHDLVTDG LITLYIETKA AEYIAKMTIN PIYEHIGYTT
160 170 180 190 200
LNREPAYKQH MAVLKETHDE KEATGQDGVS EKRLTSLVRR ATLKENEQIP
210 220 230 240 250
KYEKVHNFKV HTFRGPHWCE YCANFMWGLI AQGVKCADCG LNVHKQCSKM
260 270 280 290 300
VPNDCKPDLK HVKKVYSCDL TTLVKAHITK RPMVVDMCIR EIESRGLNSE
310 320 330 340 350
GLYRVSGFSD LIEDVKMAFD RDGEKADISV NMYEDINIIT GALKLYFRDL
360 370 380 390 400
PIPLITYDAY PKFIESAKIM DPDEQLETLH EALRSLPPAH CETLRYLMAH
410 420 430 440 450
LKRVTLHEKE NLMSAENLGI VFGPTLMRSP ELDPMAALND IRYQRLVVEL

LIKNEDILF
Length:459
Mass (Da):53,193
Last modified:September 5, 2006 - v2
Checksum:iEE10CCDA8229C0B0
GO
Isoform 2 (identifier: Q91V57-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-183: MALTLFDTDE...TGQDGVSEKR → MPSKESWSGR...QPLKLFAYSQ

Show »
Length:334
Mass (Da):38,121
Checksum:i9EC5DA66D58A36C4
GO
Isoform 3 (identifier: Q91V57-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.

Note: No experimental confirmation available.
Show »
Length:210
Mass (Da):24,096
Checksum:i57F077920F5B0326
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253N → I in AAH54770 (PubMed:15489334).Curated1
Sequence conflicti275K → E in BAE28074 (PubMed:16141072).Curated1
Sequence conflicti331N → K in BAC33996 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0201461 – 249Missing in isoform 3. 1 PublicationAdd BLAST249
Alternative sequenceiVSP_0201451 – 183MALTL…VSEKR → MPSKESWSGRKANRATVHKA KPEGRQQGLLIAALGMKLGS QKSSVTIWQPLKLFAYSQ in isoform 2. 3 PublicationsAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332069 mRNA Translation: AAK56097.1
AF332070 mRNA Translation: AAK56098.1
AK049943 mRNA Translation: BAC33996.1
AK007182 mRNA Translation: BAB24888.1
AK075606 mRNA Translation: BAC35853.1
AK147686 mRNA Translation: BAE28074.1
AL928889 Genomic DNA No translation available.
BC010825 mRNA Translation: AAH10825.1
BC024796 mRNA Translation: AAH24796.1
BC025023 mRNA Translation: AAH25023.1
BC054770 mRNA Translation: AAH54770.1
CCDSiCCDS16133.1 [Q91V57-2]
CCDS38145.1 [Q91V57-3]
CCDS50608.1 [Q91V57-1]
RefSeqiNP_001106717.2, NM_001113246.2 [Q91V57-1]
NP_083992.1, NM_029716.3 [Q91V57-3]
NP_786928.2, NM_175752.3 [Q91V57-2]
UniGeneiMm.257073

Genome annotation databases

EnsembliENSMUST00000070579; ENSMUSP00000070301; ENSMUSG00000056486 [Q91V57-3]
ENSMUST00000102677; ENSMUSP00000099738; ENSMUSG00000056486 [Q91V57-2]
ENSMUST00000112024; ENSMUSP00000107655; ENSMUSG00000056486 [Q91V57-1]
ENSMUST00000180045; ENSMUSP00000137106; ENSMUSG00000056486 [Q91V57-3]
GeneIDi108699
KEGGimmu:108699
UCSCiuc008kda.2 mouse [Q91V57-2]
uc008kdb.2 mouse [Q91V57-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCHIN_MOUSE
AccessioniPrimary (citable) accession number: Q91V57
Secondary accession number(s): A2ATM0
, Q3UGY3, Q7TQE5, Q8BWU6, Q9D9B3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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