Q91V37 (VATO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type proton ATPase 21 kDa proteolipid subunit
Short name=V-ATPase 21 kDa proteolipid subunit

Alternative name(s):
23 kDa subunit of V-ATPase
Vacuolar proton pump 21 kDa proteolipid subunit

Gene names

Name:	Atp6v0b
Synonyms:	Atp6f

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Subunit structure	Interacts with IFITM3. Ref.3
Subcellular location	Vacuole membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitous. Ref.2
Sequence similarities	Belongs to the V-ATPase proteolipid subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from sequence alignment Ref.2. Source: MGI
Cellular_component	endosome Inferred from direct assay Ref.1. Source: MGI integral to membrane Inferred from direct assay Ref.2. Source: MGI proton-transporting V-type ATPase, V0 domain Inferred from electronic annotation. Source: InterPro vacuolar proton-transporting V-type ATPase complex Inferred from sequence alignment Ref.2. Source: MGI
Molecular_function	hydrogen-exporting ATPase activity, phosphorylative mechanism Inferred from sequence alignment Ref.2. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	V-type proton ATPase 21 kDa proteolipid subunit		PRO_0000071778
Regions
	Topological domain	1 – 3	3	Lumenal Potential
	Transmembrane	4 – 24	21	Helical; Potential
	Topological domain	25 – 51	27	Cytoplasmic Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Topological domain	73 – 90	18	Lumenal Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Topological domain	112 – 137	26	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Topological domain	159 – 175	17	Lumenal Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Topological domain	197 – 205	9	Cytoplasmic Potential
Sites
	Site	98	1	Essential for proton translocation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91V37 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: C1B05E87B53C076E	FASTA	205	21,607
	10 20 30 40 50 60 MTGLELLYLG IFVAFWACMV VVGICYTIFD LGFRFDVAWF LTETSPFMWS NLGIGLAISL 70 80 90 100 110 120 SVVGAAWGIY ITGSSIIGGG VKAPRIKTKN LVSIIFCEAV AIYGIIMAIV ISNMAEPFSA 130 140 150 160 170 180 TEPKAIGHRN YHAGYSMFGA GLTVGLSNLF CGVCVGIVGS GAALADAQNP SLFVKILIVE 190 200 IFGSAIGLFG VIVAILQTSR VKMGD

References

[1]	"Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase." Sun-Wada G.H., Murakami H., Nakai H., Wada Y., Futai M. Gene 274:93-99(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/Sv and C57BL/6.
[2]	"Expression and localization of the mouse homolog of the yeast V-ATPase 21-kDa subunit c' (Vma16p)." Nishi T., Kawasaki-Nishi S., Forgac M. J. Biol. Chem. 276:34122-34130(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: BALB/c and C57BL/6J.
[3]	"Interferon-inducible transmembrane proteins of the innate immune response act as membrane organizers by influencing clathrin and v-ATPase localization and function." Wee Y.S., Roundy K.M., Weis J.J., Weis J.H. Inn. Immun. 18:834-845(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IFITM3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB060654 mRNA. Translation: BAB61954.1. AB060655 Genomic DNA. Translation: BAB61955.1. AF356006 mRNA. Translation: AAL02096.1. AF356007 Genomic DNA. Translation: AAL02097.1.
IPI	IPI00126015.
RefSeq	NP_291095.1. NM_033617.3.
UniGene	Mm.207052.
3D structure databases
ProteinModelPortal	Q91V37.
SMR	Q91V37. Positions 49-112, 131-198.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036380; ENSMUSP00000047682; ENSMUSG00000033379.
GeneID	114143.
KEGG	mmu:114143.
Organism-specific databases
CTD	533.
MGI	MGI:1890510. Atp6v0b.
Phylogenomic databases
eggNOG	COG0636.
GeneTree	ENSGT00550000075120.
HOGENOM	HOG000056522.
HOVERGEN	HBG105033.
InParanoid	Q91V37.
KO	K03661.
OMA	WGIFTTG.
OrthoDB	EOG4ZW5C4.
Gene expression databases
ArrayExpress	Q91V37.
Bgee	Q91V37.
CleanEx	MM_ATP6V0B.
Genevestigator	Q91V37.
GermOnline	ENSMUSG00000033379. Mus musculus.
Family and domain databases
InterPro	IPR002379. ATPase_proteolipid_c_like_dom. IPR000245. ATPase_proteolipid_csu. [Graphical view]
Pfam	PF00137. ATP-synt_C. 2 hits. [Graphical view]
PRINTS	PR00122. VACATPASE.
SUPFAM	SSF81333. ATPase_F0/V0_c. 2 hits.
ProtoNet	Search...
Other
ChiTaRS	ATP6V0B. mouse.
NextBio	368143.
SOURCE	Search...

Entry information

Entry name

VATO_MOUSE

Accession

Primary (citable) accession number: Q91V37

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program