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Protein

Melanophilin

Gene

Mlph

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri64 – 107FYVE-typeAdd BLAST44

GO - Molecular functioni

  • actin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • microtubule plus-end binding Source: MGI
  • myosin binding Source: MGI
  • myosin V binding Source: MGI
  • Rab GTPase binding Source: MGI

GO - Biological processi

  • melanocyte differentiation Source: MGI
  • melanosome localization Source: MGI
  • pigmentation Source: MGI
  • protein targeting Source: MGI
  • vesicle transport along actin filament Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Melanophilin
Alternative name(s):
Exophilin-3
Leaden protein
Slp homolog lacking C2 domains a
Short name:
SlaC2-a
Synaptotagmin-like protein 2a
Gene namesi
Name:Mlph
Synonyms:Ln, Slac2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2176380. Mlph.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cortical actin cytoskeleton Source: MGI
  • dendrite Source: MGI
  • extracellular exosome Source: MGI
  • melanosome Source: MGI
  • microtubule organizing center Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • stress fiber Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902231 – 590MelanophilinAdd BLAST590

Proteomic databases

MaxQBiQ91V27.
PaxDbiQ91V27.
PRIDEiQ91V27.

PTM databases

iPTMnetiQ91V27.
PhosphoSitePlusiQ91V27.

Expressioni

Tissue specificityi

Highly expressed in embryos at day 7; not detectable at day 11. Highly expressed in adult stomach; detected at lower levels in kidney, lung, skin and small intestine. Detected in melanocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000026303.
CleanExiMM_MLPH.

Interactioni

Subunit structurei

Binds RAB27A that has been activated by GTP-binding via its N-terminus. Binds MYO5A via its C-terminal coiled coil domain.2 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • microtubule plus-end binding Source: MGI
  • myosin binding Source: MGI
  • myosin V binding Source: MGI
  • Rab GTPase binding Source: MGI

Protein-protein interaction databases

BioGridi228608. 1 interactor.
DIPiDIP-31495N.
IntActiQ91V27. 1 interactor.
STRINGi10090.ENSMUSP00000027528.

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 53Combined sources42
Helixi59 – 61Combined sources3
Turni65 – 67Combined sources3
Helixi71 – 73Combined sources3
Turni82 – 84Combined sources3
Helixi90 – 92Combined sources3
Beta strandi97 – 101Combined sources5
Helixi105 – 117Combined sources13
Helixi119 – 126Combined sources8
Beta strandi129 – 131Combined sources3
Helixi133 – 140Combined sources8
Helixi193 – 195Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZETX-ray3.00C/D1-146[»]
4KP3X-ray2.40E/F170-208[»]
4LX2X-ray1.50B176-201[»]
DisProtiDP00541.
ProteinModelPortaliQ91V27.
SMRiQ91V27.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91V27.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 124RabBDPROSITE-ProRule annotationAdd BLAST121

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili339 – 485Sequence analysisAdd BLAST147

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.Curated
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri64 – 107FYVE-typeAdd BLAST44

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEPI. Eukaryota.
ENOG41102EA. LUCA.
HOGENOMiHOG000089970.
HOVERGENiHBG052454.
InParanoidiQ91V27.
PhylomeDBiQ91V27.
TreeFamiTF331599.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR006788. Myrip/Melanophilin.
IPR010911. Rab_BD.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02318. FYVE_2. 1 hit.
PF04698. Rab_eff_C. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50916. RABBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91V27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRLDLSTL TDEEAEHVWA VVQRDFDLRR REEERLQGLK GKIQKESSKR
60 70 80 90 100
ELLSDTAHLN ETHCARCLQP YRLLLNSRRQ CLECSLFVCK SCSHAHPEEQ
110 120 130 140 150
GWLCDPCHLA RVVKIGSLEW YYQHVRARFK RFGSAKVIRS LCGRLQGGGG
160 170 180 190 200
SEPSLEEGNG DSEQTDEDGD LDTEARDQPL NSKKKKRLLS FRDVDFEEDS
210 220 230 240 250
DHLVQPCSQT LGLSSVPESA HSLQSLSGEP YSEDTTSLEP EGLEETGARA
260 270 280 290 300
LGCRPSPEVQ PCSPLPSGED AHAELDSPAA SCKSAFGTTA MPGTDDVRGK
310 320 330 340 350
HLPSQYLADV DTSDEDSIQG PRAASQHSKR RARTVPETQI LELNKRMSAV
360 370 380 390 400
EHLLVHLENT VLPPSAQEPT VETHPSADTE EETLRRRLEE LTSNISGSST
410 420 430 440 450
SSEDETKPDG TFLGGSPKVC TDTGHMETQE RNPRSPGNPA RPTKSTDEEL
460 470 480 490 500
SEMEDRVAMT ASEVQQAESE ISDIESRIAA LRAAGLTVKP SGKPRRKSGI
510 520 530 540 550
PIFLPRVTEK LDRIPKTPPA DPDDQAKMPK ATTAVPSLLR RKYSPSSQGV
560 570 580 590
DSGSFDRKSV YRGSLTQRNP NGRRGTARHI FAKPVMAQQP
Length:590
Mass (Da):65,052
Last modified:December 1, 2001 - v1
Checksum:iC91444B16B8EFA1D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192R → Q in BAE28452 (PubMed:16141072).Curated1
Sequence conflicti254R → H in BAB41087 (PubMed:11327731).Curated1
Sequence conflicti333R → W in BAE28452 (PubMed:16141072).Curated1
Sequence conflicti360T → R in BAE28452 (PubMed:16141072).Curated1
Sequence conflicti475E → Q in BAB41087 (PubMed:11327731).Curated1
Sequence conflicti499G → S in BAE28452 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057759 mRNA. Translation: BAB41087.1.
AF384098 mRNA. Translation: AAK97435.1.
AK148274 mRNA. Translation: BAE28452.1.
CCDSiCCDS15155.1.
RefSeqiNP_443748.2. NM_053015.3.
UniGeneiMm.105208.

Genome annotation databases

GeneIDi171531.
KEGGimmu:171531.
UCSCiuc007bzm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057759 mRNA. Translation: BAB41087.1.
AF384098 mRNA. Translation: AAK97435.1.
AK148274 mRNA. Translation: BAE28452.1.
CCDSiCCDS15155.1.
RefSeqiNP_443748.2. NM_053015.3.
UniGeneiMm.105208.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZETX-ray3.00C/D1-146[»]
4KP3X-ray2.40E/F170-208[»]
4LX2X-ray1.50B176-201[»]
DisProtiDP00541.
ProteinModelPortaliQ91V27.
SMRiQ91V27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228608. 1 interactor.
DIPiDIP-31495N.
IntActiQ91V27. 1 interactor.
STRINGi10090.ENSMUSP00000027528.

PTM databases

iPTMnetiQ91V27.
PhosphoSitePlusiQ91V27.

Proteomic databases

MaxQBiQ91V27.
PaxDbiQ91V27.
PRIDEiQ91V27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171531.
KEGGimmu:171531.
UCSCiuc007bzm.2. mouse.

Organism-specific databases

CTDi79083.
MGIiMGI:2176380. Mlph.

Phylogenomic databases

eggNOGiENOG410IEPI. Eukaryota.
ENOG41102EA. LUCA.
HOGENOMiHOG000089970.
HOVERGENiHBG052454.
InParanoidiQ91V27.
PhylomeDBiQ91V27.
TreeFamiTF331599.

Miscellaneous databases

ChiTaRSiMlph. mouse.
EvolutionaryTraceiQ91V27.
PROiQ91V27.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026303.
CleanExiMM_MLPH.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR006788. Myrip/Melanophilin.
IPR010911. Rab_BD.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02318. FYVE_2. 1 hit.
PF04698. Rab_eff_C. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50916. RABBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMELPH_MOUSE
AccessioniPrimary (citable) accession number: Q91V27
Secondary accession number(s): Q3UFV7, Q99N53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.