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Q91V27

- MELPH_MOUSE

UniProt

Q91V27 - MELPH_MOUSE

Protein

Melanophilin

Gene

Mlph

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri64 – 10744FYVE-typeAdd
    BLAST

    GO - Molecular functioni

    1. actin binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. microtubule plus-end binding Source: MGI
    4. myosin binding Source: MGI
    5. myosin V binding Source: MGI
    6. protein binding Source: UniProtKB
    7. Rab GTPase binding Source: MGI

    GO - Biological processi

    1. melanocyte differentiation Source: MGI
    2. melanosome localization Source: MGI
    3. pigmentation Source: MGI
    4. protein targeting Source: MGI

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Melanophilin
    Alternative name(s):
    Exophilin-3
    Leaden protein
    Slp homolog lacking C2 domains a
    Short name:
    SlaC2-a
    Synaptotagmin-like protein 2a
    Gene namesi
    Name:Mlph
    Synonyms:Ln, Slac2a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2176380. Mlph.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cytoskeleton Source: MGI
    2. cortical actin cytoskeleton Source: MGI
    3. melanosome Source: MGI
    4. microtubule organizing center Source: MGI
    5. stress fiber Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 590590MelanophilinPRO_0000190223Add
    BLAST

    Proteomic databases

    PaxDbiQ91V27.
    PRIDEiQ91V27.

    PTM databases

    PhosphoSiteiQ91V27.

    Expressioni

    Tissue specificityi

    Highly expressed in embryos at day 7; not detectable at day 11. Highly expressed in adult stomach; detected at lower levels in kidney, lung, skin and small intestine. Detected in melanocytes.1 Publication

    Gene expression databases

    ArrayExpressiQ91V27.
    BgeeiQ91V27.
    CleanExiMM_MLPH.
    GenevestigatoriQ91V27.

    Interactioni

    Subunit structurei

    Binds RAB27A that has been activated by GTP-binding via its N-terminus. Binds MYO5A via its C-terminal coiled coil domain.2 Publications

    Protein-protein interaction databases

    BioGridi228608. 1 interaction.
    DIPiDIP-31495N.
    IntActiQ91V27. 1 interaction.

    Structurei

    Secondary structure

    1
    590
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi12 – 5342
    Helixi59 – 613
    Turni65 – 673
    Helixi71 – 733
    Turni82 – 843
    Helixi90 – 923
    Beta strandi97 – 1015
    Helixi105 – 11713
    Helixi119 – 1268
    Beta strandi129 – 1313
    Helixi133 – 1408
    Helixi193 – 1953

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZETX-ray3.00C/D1-146[»]
    4KP3X-ray2.40E/F170-208[»]
    4LX2X-ray1.50B176-201[»]
    DisProtiDP00541.
    ProteinModelPortaliQ91V27.
    SMRiQ91V27. Positions 4-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ91V27.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 124121RabBDPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili339 – 485147Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FYVE-type zinc finger.Curated
    Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri64 – 10744FYVE-typeAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG135976.
    GeneTreeiENSGT00390000013933.
    HOGENOMiHOG000089970.
    HOVERGENiHBG052454.
    InParanoidiQ91V27.
    OrthoDBiEOG76HQ10.
    PhylomeDBiQ91V27.
    TreeFamiTF331599.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR006788. Myrip/Melanophilin.
    IPR010911. Znf_FYVE-typ.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF02318. FYVE_2. 1 hit.
    PF04698. Rab_eff_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF57903. SSF57903. 1 hit.
    PROSITEiPS50916. RABBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91V27-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKRLDLSTL TDEEAEHVWA VVQRDFDLRR REEERLQGLK GKIQKESSKR    50
    ELLSDTAHLN ETHCARCLQP YRLLLNSRRQ CLECSLFVCK SCSHAHPEEQ 100
    GWLCDPCHLA RVVKIGSLEW YYQHVRARFK RFGSAKVIRS LCGRLQGGGG 150
    SEPSLEEGNG DSEQTDEDGD LDTEARDQPL NSKKKKRLLS FRDVDFEEDS 200
    DHLVQPCSQT LGLSSVPESA HSLQSLSGEP YSEDTTSLEP EGLEETGARA 250
    LGCRPSPEVQ PCSPLPSGED AHAELDSPAA SCKSAFGTTA MPGTDDVRGK 300
    HLPSQYLADV DTSDEDSIQG PRAASQHSKR RARTVPETQI LELNKRMSAV 350
    EHLLVHLENT VLPPSAQEPT VETHPSADTE EETLRRRLEE LTSNISGSST 400
    SSEDETKPDG TFLGGSPKVC TDTGHMETQE RNPRSPGNPA RPTKSTDEEL 450
    SEMEDRVAMT ASEVQQAESE ISDIESRIAA LRAAGLTVKP SGKPRRKSGI 500
    PIFLPRVTEK LDRIPKTPPA DPDDQAKMPK ATTAVPSLLR RKYSPSSQGV 550
    DSGSFDRKSV YRGSLTQRNP NGRRGTARHI FAKPVMAQQP 590
    Length:590
    Mass (Da):65,052
    Last modified:December 1, 2001 - v1
    Checksum:iC91444B16B8EFA1D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti192 – 1921R → Q in BAE28452. (PubMed:16141072)Curated
    Sequence conflicti254 – 2541R → H in BAB41087. (PubMed:11327731)Curated
    Sequence conflicti333 – 3331R → W in BAE28452. (PubMed:16141072)Curated
    Sequence conflicti360 – 3601T → R in BAE28452. (PubMed:16141072)Curated
    Sequence conflicti475 – 4751E → Q in BAB41087. (PubMed:11327731)Curated
    Sequence conflicti499 – 4991G → S in BAE28452. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB057759 mRNA. Translation: BAB41087.1.
    AF384098 mRNA. Translation: AAK97435.1.
    AK148274 mRNA. Translation: BAE28452.1.
    CCDSiCCDS15155.1.
    RefSeqiNP_443748.2. NM_053015.3.
    UniGeneiMm.105208.

    Genome annotation databases

    EnsembliENSMUST00000027528; ENSMUSP00000027528; ENSMUSG00000026303.
    GeneIDi171531.
    KEGGimmu:171531.
    UCSCiuc007bzm.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB057759 mRNA. Translation: BAB41087.1 .
    AF384098 mRNA. Translation: AAK97435.1 .
    AK148274 mRNA. Translation: BAE28452.1 .
    CCDSi CCDS15155.1.
    RefSeqi NP_443748.2. NM_053015.3.
    UniGenei Mm.105208.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZET X-ray 3.00 C/D 1-146 [» ]
    4KP3 X-ray 2.40 E/F 170-208 [» ]
    4LX2 X-ray 1.50 B 176-201 [» ]
    DisProti DP00541.
    ProteinModelPortali Q91V27.
    SMRi Q91V27. Positions 4-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 228608. 1 interaction.
    DIPi DIP-31495N.
    IntActi Q91V27. 1 interaction.

    PTM databases

    PhosphoSitei Q91V27.

    Proteomic databases

    PaxDbi Q91V27.
    PRIDEi Q91V27.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027528 ; ENSMUSP00000027528 ; ENSMUSG00000026303 .
    GeneIDi 171531.
    KEGGi mmu:171531.
    UCSCi uc007bzm.2. mouse.

    Organism-specific databases

    CTDi 79083.
    MGIi MGI:2176380. Mlph.

    Phylogenomic databases

    eggNOGi NOG135976.
    GeneTreei ENSGT00390000013933.
    HOGENOMi HOG000089970.
    HOVERGENi HBG052454.
    InParanoidi Q91V27.
    OrthoDBi EOG76HQ10.
    PhylomeDBi Q91V27.
    TreeFami TF331599.

    Miscellaneous databases

    ChiTaRSi MLPH. mouse.
    EvolutionaryTracei Q91V27.
    NextBioi 371036.
    PROi Q91V27.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91V27.
    Bgeei Q91V27.
    CleanExi MM_MLPH.
    Genevestigatori Q91V27.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR006788. Myrip/Melanophilin.
    IPR010911. Znf_FYVE-typ.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF02318. FYVE_2. 1 hit.
    PF04698. Rab_eff_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57903. SSF57903. 1 hit.
    PROSITEi PS50916. RABBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain."
      Fukuda M., Saegusa C., Mikoshiba K.
      Biochem. Biophys. Res. Commun. 283:513-519(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Brain.
    2. "Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice."
      Matesic L.E., Yip R., Reuss A.E., Swing D.A., O'Sullivan T.N., Fletcher C.F., Copeland N.G., Jenkins N.A.
      Proc. Natl. Acad. Sci. U.S.A. 98:10238-10243(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Melanoma.
    4. Cited for: INTERACTION WITH RAB27A AND MYO5A, FUNCTION.
    5. "Structural basis for the exclusive specificity of Slac2-a/melanophilin for the Rab27 GTPases."
      Kukimoto-Niino M., Sakamoto A., Kanno E., Hanawa-Suetsugu K., Terada T., Shirouzu M., Fukuda M., Yokoyama S.
      Structure 16:1478-1490(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-146 IN COMPLEX WITH RAB27B, SUBUNIT.
    6. "Structural basis of cargo recognitions for class V myosins."
      Wei Z., Liu X., Yu C., Zhang M.
      Proc. Natl. Acad. Sci. U.S.A. 110:11314-11319(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 170-208 IN COMPLEX WITH MYO5A, SUBUNIT.

    Entry informationi

    Entry nameiMELPH_MOUSE
    AccessioniPrimary (citable) accession number: Q91V27
    Secondary accession number(s): Q3UFV7, Q99N53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3