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Q91V27

- MELPH_MOUSE

UniProt

Q91V27 - MELPH_MOUSE

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Protein

Melanophilin

Gene

Mlph

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri64 – 10744FYVE-typeAdd
BLAST

GO - Molecular functioni

  1. actin binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. microtubule plus-end binding Source: MGI
  4. myosin binding Source: MGI
  5. myosin V binding Source: MGI
  6. Rab GTPase binding Source: MGI

GO - Biological processi

  1. melanocyte differentiation Source: MGI
  2. melanosome localization Source: MGI
  3. pigmentation Source: MGI
  4. protein targeting Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Melanophilin
Alternative name(s):
Exophilin-3
Leaden protein
Slp homolog lacking C2 domains a
Short name:
SlaC2-a
Synaptotagmin-like protein 2a
Gene namesi
Name:Mlph
Synonyms:Ln, Slac2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2176380. Mlph.

Subcellular locationi

GO - Cellular componenti

  1. actin cytoskeleton Source: MGI
  2. cortical actin cytoskeleton Source: MGI
  3. melanosome Source: MGI
  4. microtubule organizing center Source: MGI
  5. stress fiber Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590MelanophilinPRO_0000190223Add
BLAST

Proteomic databases

PaxDbiQ91V27.
PRIDEiQ91V27.

PTM databases

PhosphoSiteiQ91V27.

Expressioni

Tissue specificityi

Highly expressed in embryos at day 7; not detectable at day 11. Highly expressed in adult stomach; detected at lower levels in kidney, lung, skin and small intestine. Detected in melanocytes.1 Publication

Gene expression databases

BgeeiQ91V27.
CleanExiMM_MLPH.
ExpressionAtlasiQ91V27. baseline and differential.
GenevestigatoriQ91V27.

Interactioni

Subunit structurei

Binds RAB27A that has been activated by GTP-binding via its N-terminus. Binds MYO5A via its C-terminal coiled coil domain.2 Publications

Protein-protein interaction databases

BioGridi228608. 1 interaction.
DIPiDIP-31495N.
IntActiQ91V27. 1 interaction.

Structurei

Secondary structure

1
590
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 5342Combined sources
Helixi59 – 613Combined sources
Turni65 – 673Combined sources
Helixi71 – 733Combined sources
Turni82 – 843Combined sources
Helixi90 – 923Combined sources
Beta strandi97 – 1015Combined sources
Helixi105 – 11713Combined sources
Helixi119 – 1268Combined sources
Beta strandi129 – 1313Combined sources
Helixi133 – 1408Combined sources
Helixi193 – 1953Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZETX-ray3.00C/D1-146[»]
4KP3X-ray2.40E/F170-208[»]
4LX2X-ray1.50B176-201[»]
DisProtiDP00541.
ProteinModelPortaliQ91V27.
SMRiQ91V27. Positions 4-144.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91V27.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 124121RabBDPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili339 – 485147Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.Curated
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri64 – 10744FYVE-typeAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG135976.
GeneTreeiENSGT00390000013933.
HOGENOMiHOG000089970.
HOVERGENiHBG052454.
InParanoidiQ91V27.
OrthoDBiEOG76HQ10.
PhylomeDBiQ91V27.
TreeFamiTF331599.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR006788. Myrip/Melanophilin.
IPR010911. Znf_FYVE-typ.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02318. FYVE_2. 1 hit.
PF04698. Rab_eff_C. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50916. RABBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91V27-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKRLDLSTL TDEEAEHVWA VVQRDFDLRR REEERLQGLK GKIQKESSKR
60 70 80 90 100
ELLSDTAHLN ETHCARCLQP YRLLLNSRRQ CLECSLFVCK SCSHAHPEEQ
110 120 130 140 150
GWLCDPCHLA RVVKIGSLEW YYQHVRARFK RFGSAKVIRS LCGRLQGGGG
160 170 180 190 200
SEPSLEEGNG DSEQTDEDGD LDTEARDQPL NSKKKKRLLS FRDVDFEEDS
210 220 230 240 250
DHLVQPCSQT LGLSSVPESA HSLQSLSGEP YSEDTTSLEP EGLEETGARA
260 270 280 290 300
LGCRPSPEVQ PCSPLPSGED AHAELDSPAA SCKSAFGTTA MPGTDDVRGK
310 320 330 340 350
HLPSQYLADV DTSDEDSIQG PRAASQHSKR RARTVPETQI LELNKRMSAV
360 370 380 390 400
EHLLVHLENT VLPPSAQEPT VETHPSADTE EETLRRRLEE LTSNISGSST
410 420 430 440 450
SSEDETKPDG TFLGGSPKVC TDTGHMETQE RNPRSPGNPA RPTKSTDEEL
460 470 480 490 500
SEMEDRVAMT ASEVQQAESE ISDIESRIAA LRAAGLTVKP SGKPRRKSGI
510 520 530 540 550
PIFLPRVTEK LDRIPKTPPA DPDDQAKMPK ATTAVPSLLR RKYSPSSQGV
560 570 580 590
DSGSFDRKSV YRGSLTQRNP NGRRGTARHI FAKPVMAQQP
Length:590
Mass (Da):65,052
Last modified:December 1, 2001 - v1
Checksum:iC91444B16B8EFA1D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921R → Q in BAE28452. (PubMed:16141072)Curated
Sequence conflicti254 – 2541R → H in BAB41087. (PubMed:11327731)Curated
Sequence conflicti333 – 3331R → W in BAE28452. (PubMed:16141072)Curated
Sequence conflicti360 – 3601T → R in BAE28452. (PubMed:16141072)Curated
Sequence conflicti475 – 4751E → Q in BAB41087. (PubMed:11327731)Curated
Sequence conflicti499 – 4991G → S in BAE28452. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057759 mRNA. Translation: BAB41087.1.
AF384098 mRNA. Translation: AAK97435.1.
AK148274 mRNA. Translation: BAE28452.1.
CCDSiCCDS15155.1.
RefSeqiNP_443748.2. NM_053015.3.
UniGeneiMm.105208.

Genome annotation databases

EnsembliENSMUST00000027528; ENSMUSP00000027528; ENSMUSG00000026303.
GeneIDi171531.
KEGGimmu:171531.
UCSCiuc007bzm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057759 mRNA. Translation: BAB41087.1 .
AF384098 mRNA. Translation: AAK97435.1 .
AK148274 mRNA. Translation: BAE28452.1 .
CCDSi CCDS15155.1.
RefSeqi NP_443748.2. NM_053015.3.
UniGenei Mm.105208.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZET X-ray 3.00 C/D 1-146 [» ]
4KP3 X-ray 2.40 E/F 170-208 [» ]
4LX2 X-ray 1.50 B 176-201 [» ]
DisProti DP00541.
ProteinModelPortali Q91V27.
SMRi Q91V27. Positions 4-144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 228608. 1 interaction.
DIPi DIP-31495N.
IntActi Q91V27. 1 interaction.

PTM databases

PhosphoSitei Q91V27.

Proteomic databases

PaxDbi Q91V27.
PRIDEi Q91V27.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027528 ; ENSMUSP00000027528 ; ENSMUSG00000026303 .
GeneIDi 171531.
KEGGi mmu:171531.
UCSCi uc007bzm.2. mouse.

Organism-specific databases

CTDi 79083.
MGIi MGI:2176380. Mlph.

Phylogenomic databases

eggNOGi NOG135976.
GeneTreei ENSGT00390000013933.
HOGENOMi HOG000089970.
HOVERGENi HBG052454.
InParanoidi Q91V27.
OrthoDBi EOG76HQ10.
PhylomeDBi Q91V27.
TreeFami TF331599.

Miscellaneous databases

ChiTaRSi Mlph. mouse.
EvolutionaryTracei Q91V27.
NextBioi 371036.
PROi Q91V27.
SOURCEi Search...

Gene expression databases

Bgeei Q91V27.
CleanExi MM_MLPH.
ExpressionAtlasi Q91V27. baseline and differential.
Genevestigatori Q91V27.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR006788. Myrip/Melanophilin.
IPR010911. Znf_FYVE-typ.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF02318. FYVE_2. 1 hit.
PF04698. Rab_eff_C. 1 hit.
[Graphical view ]
SUPFAMi SSF57903. SSF57903. 1 hit.
PROSITEi PS50916. RABBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain."
    Fukuda M., Saegusa C., Mikoshiba K.
    Biochem. Biophys. Res. Commun. 283:513-519(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  2. "Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice."
    Matesic L.E., Yip R., Reuss A.E., Swing D.A., O'Sullivan T.N., Fletcher C.F., Copeland N.G., Jenkins N.A.
    Proc. Natl. Acad. Sci. U.S.A. 98:10238-10243(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Melanoma.
  4. Cited for: INTERACTION WITH RAB27A AND MYO5A, FUNCTION.
  5. "Structural basis for the exclusive specificity of Slac2-a/melanophilin for the Rab27 GTPases."
    Kukimoto-Niino M., Sakamoto A., Kanno E., Hanawa-Suetsugu K., Terada T., Shirouzu M., Fukuda M., Yokoyama S.
    Structure 16:1478-1490(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-146 IN COMPLEX WITH RAB27B, SUBUNIT.
  6. "Structural basis of cargo recognitions for class V myosins."
    Wei Z., Liu X., Yu C., Zhang M.
    Proc. Natl. Acad. Sci. U.S.A. 110:11314-11319(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 170-208 IN COMPLEX WITH MYO5A, SUBUNIT.

Entry informationi

Entry nameiMELPH_MOUSE
AccessioniPrimary (citable) accession number: Q91V27
Secondary accession number(s): Q3UFV7, Q99N53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3