Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sphingosine kinase 1

Gene

Sphk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (By similarity).By similarity

Catalytic activityi

ATP + sphinganine = ADP + sphinganine 1-phosphate.
ATP + sphingosine = ADP + sphingosine 1-phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811Proton donor/acceptorBy similarity
Binding sitei86 – 861ATPPROSITE-ProRule annotation
Binding sitei178 – 1781SubstrateBy similarity
Binding sitei185 – 1851ATPPROSITE-ProRule annotation
Binding sitei191 – 1911ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 243ATPPROSITE-ProRule annotation
Nucleotide bindingi54 – 585ATPPROSITE-ProRule annotation
Nucleotide bindingi111 – 1133ATPPROSITE-ProRule annotation
Nucleotide bindingi340 – 3423ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to growth factor stimulus Source: RGD
  • cellular response to hydrogen peroxide Source: RGD
  • cellular response to starvation Source: RGD
  • cyclooxygenase pathway Source: RGD
  • female pregnancy Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of cell migration Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of neurotransmitter secretion Source: RGD
  • positive regulation of NF-kappaB import into nucleus Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of protein ubiquitination Source: UniProtKB
  • regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
  • response to amine Source: RGD
  • response to ATP Source: RGD
  • response to interleukin-1 Source: RGD
  • response to magnesium ion Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to progesterone Source: RGD
  • sphingosine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.91. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine kinase 1 (EC:2.7.1.91)
Short name:
SK 1
Short name:
SPK 1
Gene namesi
Name:Sphk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620048. Sphk1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Cell membrane

  • Note: Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.By similarity

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
  • neuron projection Source: RGD
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
  • synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075107.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Sphingosine kinase 1PRO_0000333033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei193 – 1931PhosphothreonineBy similarity
Modified residuei225 – 2251PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91V26.
PRIDEiQ91V26.

PTM databases

PhosphoSiteiQ91V26.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010626.

Interactioni

Subunit structurei

Interacts with CIB1, the interaction occurs in a calcium-dependent manner (By similarity). Binds to calmodulin. Interacts with SPHKAP. Interacts with ACY1.By similarity

Protein-protein interaction databases

IntActiQ91V26. 1 interaction.
STRINGi10116.ENSRNOP00000029296.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 159148DAGKcPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 824Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi147 – 1559Nuclear export signal 1By similarity
Motifi161 – 1699Nuclear export signal 2By similarity

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1116. Eukaryota.
COG1597. LUCA.
HOVERGENiHBG054796.
InParanoidiQ91V26.
KOiK04718.
PhylomeDBiQ91V26.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91V26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPADCPRGL LPRPCRVLVL LNPRGGKGKA LKLFQSRVRP LLEEAEVSFK
60 70 80 90 100
LMLTERQNHA RELVCAEELG HWDALAVMSG DGLMHEVVNG LMERPDWESA
110 120 130 140 150
IQKPLCSLPG GSGNALAASL NYYAGHEQVT NEDLLINCTL LLCCRQLSPM
160 170 180 190 200
NLLSLHTASG RQLYSVLSLS WGFVADVDLE SEKYRSLGEI RFTVGTFFRL
210 220 230 240 250
ASLRIYQGQL AYLPVGKAAS KIPASSLAQK GPANTYLVPL EEPVPPHWTV
260 270 280 290 300
VPEQDFVLVL VLLHTHLSTE MFAAPMGRCE AGVMHLFYIR AGVSRAMLVR
310 320 330 340 350
LFLAMQKGKH MDLDCPYLVH VPVVAFRLEP RNQRGVFSVD GELMVCEAVQ
360 370 380
GQVHPNYLWM VSGSSDSPSG RDSQRRPPPE EPI
Length:383
Mass (Da):42,418
Last modified:December 1, 2001 - v1
Checksum:iCAAD5817BF4B9507
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 2991V → L in AAH81738 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049571 mRNA. Translation: BAB62320.1.
AB049572 mRNA. Translation: BAB62321.1.
AB049573 mRNA. Translation: BAB62322.1.
AB049574 mRNA. Translation: BAB62323.1.
AB049575 mRNA. Translation: BAB62324.1.
BC081738 mRNA. Translation: AAH81738.1.
RefSeqiNP_001257736.1. NM_001270807.1.
NP_001257737.1. NM_001270808.1.
NP_001257738.1. NM_001270809.1.
NP_001257739.1. NM_001270810.1.
NP_001257740.1. NM_001270811.1.
NP_596877.2. NM_133386.3.
XP_006247850.1. XM_006247788.2.
UniGeneiRn.18522.

Genome annotation databases

GeneIDi170897.
KEGGirno:170897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049571 mRNA. Translation: BAB62320.1.
AB049572 mRNA. Translation: BAB62321.1.
AB049573 mRNA. Translation: BAB62322.1.
AB049574 mRNA. Translation: BAB62323.1.
AB049575 mRNA. Translation: BAB62324.1.
BC081738 mRNA. Translation: AAH81738.1.
RefSeqiNP_001257736.1. NM_001270807.1.
NP_001257737.1. NM_001270808.1.
NP_001257738.1. NM_001270809.1.
NP_001257739.1. NM_001270810.1.
NP_001257740.1. NM_001270811.1.
NP_596877.2. NM_133386.3.
XP_006247850.1. XM_006247788.2.
UniGeneiRn.18522.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91V26. 1 interaction.
STRINGi10116.ENSRNOP00000029296.

Chemistry

ChEMBLiCHEMBL1075107.

PTM databases

PhosphoSiteiQ91V26.

Proteomic databases

PaxDbiQ91V26.
PRIDEiQ91V26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi170897.
KEGGirno:170897.

Organism-specific databases

CTDi8877.
RGDi620048. Sphk1.

Phylogenomic databases

eggNOGiKOG1116. Eukaryota.
COG1597. LUCA.
HOVERGENiHBG054796.
InParanoidiQ91V26.
KOiK04718.
PhylomeDBiQ91V26.

Enzyme and pathway databases

BRENDAi2.7.1.91. 5301.

Miscellaneous databases

PROiQ91V26.

Gene expression databases

BgeeiENSRNOG00000010626.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPHK1_RAT
AccessioniPrimary (citable) accession number: Q91V26
Secondary accession number(s): Q642F6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Sphingosine 1-phosphate stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.