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Q91V24 (ABCA7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-binding cassette sub-family A member 7
Gene names
Name:Abca7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocytes differentiation. Ref.3 Ref.5 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Note: Localizes to cell membrane ruffles and phagocytic cups of macrophages stimulated with C1q or apoptotic cells. Localizes to the cytoplasm of resting macrophages, probably in Golgi and endosomes. Localizes to the apical brush border of cells in the proximal tubules of kidney. Ref.3 Ref.6

Tissue specificity

Widely expressed with higher expression in brain, lung, adrenal gland and spleen (at protein level). Highly expressed in spleen and hematopoietic tissues. Ref.1 Ref.3 Ref.5

Developmental stage

Widely expressed during embryogenesis. Ref.1

Induction

Down-regulated by digoxin. Ref.4

Disruption phenotype

According to Ref.6, mice are not viable and heterozygous knockout mice display impaired phagocytosis of apoptotic cells. Depletion of Abca7 in macrophages by RNAi reduces phagocytosis of apoptotic cells. According to Ref.5, mice are viable and females have less visceral fat and lower total serum and high density lipoprotein cholesterol level. Ref.5 Ref.6

Sequence similarities

Belongs to the ABC transporter superfamily. ABCA family.

Contains 2 ABC transporter domains.

Sequence caution

The sequence AAF31434.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
   Biological processPhagocytosis
Transport
   Cellular componentCell membrane
Endosome
Golgi apparatus
Membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapolipoprotein A-I-mediated signaling pathway

Inferred from direct assay Ref.3. Source: Alzheimers_University_of_Toronto

cholesterol efflux

Inferred from sequence or structural similarity. Source: Alzheimers_University_of_Toronto

high-density lipoprotein particle assembly

Inferred from sequence or structural similarity. Source: Alzheimers_University_of_Toronto

memory

Inferred from mutant phenotype PubMed 23467355. Source: Alzheimers_University_of_Toronto

negative regulation of ATPase activity

Inferred from sequence or structural similarity. Source: Alzheimers_University_of_Toronto

negative regulation of amyloid precursor protein biosynthetic process

Inferred from direct assay PubMed 18429932. Source: Alzheimers_University_of_Toronto

negative regulation of beta-amyloid formation

Inferred from direct assay PubMed 18429932. Source: Alzheimers_University_of_Toronto

peptide cross-linking

Inferred from direct assay Ref.3. Source: Alzheimers_University_of_Toronto

phagocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

phospholipid efflux

Inferred from direct assay PubMed 15520449. Source: MGI

phospholipid scrambling

Inferred from sequence or structural similarity. Source: Alzheimers_University_of_Toronto

positive regulation of ATPase activity

Inferred from sequence or structural similarity. Source: Alzheimers_University_of_Toronto

positive regulation of ERK1 and ERK2 cascade

Inferred from mutant phenotype Ref.6. Source: Alzheimers_University_of_Toronto

positive regulation of beta-amyloid clearance

Inferred from mutant phenotype PubMed 23467355. Source: Alzheimers_University_of_Toronto

positive regulation of cholesterol efflux

Inferred from direct assay PubMed 18429932. Source: Alzheimers_University_of_Toronto

positive regulation of engulfment of apoptotic cell

Inferred from mutant phenotype Ref.6. Source: Alzheimers_University_of_Toronto

positive regulation of phagocytosis

Inferred from mutant phenotype PubMed 20495215. Source: Alzheimers_University_of_Toronto

positive regulation of phospholipid efflux

Inferred from direct assay Ref.3. Source: Alzheimers_University_of_Toronto

protein localization to nucleus

Inferred from direct assay PubMed 18429932. Source: Alzheimers_University_of_Toronto

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

apical plasma membrane

Inferred from direct assay PubMed 15520449. Source: MGI

cell surface

Inferred from direct assay Ref.3PubMed 20495215. Source: Alzheimers_University_of_Toronto

endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular

Inferred from direct assay PubMed 15520449. Source: MGI

phagocytic cup

Inferred from direct assay Ref.6. Source: Alzheimers_University_of_Toronto

plasma membrane

Inferred from sequence or structural similarity. Source: Alzheimers_University_of_Toronto

ruffle membrane

Inferred from direct assay Ref.6. Source: Alzheimers_University_of_Toronto

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATPase activity

Inferred from electronic annotation. Source: InterPro

apolipoprotein A-I receptor activity

Inferred from direct assay Ref.3. Source: Alzheimers_University_of_Toronto

phospholipid transporter activity

Inferred from direct assay PubMed 15520449. Source: MGI

protein binding

Inferred from physical interaction Ref.3. Source: Alzheimers_University_of_Toronto

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21592159ATP-binding cassette sub-family A member 7
PRO_0000250675

Regions

Transmembrane22 – 4221Helical; Potential
Topological domain43 – 546504Extracellular By similarity
Transmembrane547 – 56721Helical; Potential
Transmembrane590 – 61021Helical; Potential
Transmembrane623 – 64321Helical; Potential
Transmembrane652 – 67221Helical; Potential
Transmembrane678 – 69821Helical; Potential
Transmembrane732 – 75221Helical; Potential
Transmembrane846 – 86621Helical; Potential
Transmembrane1246 – 126621Helical; Potential
Topological domain1267 – 1551285Extracellular By similarity
Transmembrane1552 – 157221Helical; Potential
Transmembrane1598 – 161821Helical; Potential
Transmembrane1635 – 165521Helical; Potential
Transmembrane1663 – 168321Helical; Potential
Transmembrane1743 – 176321Helical; Potential
Domain804 – 1035232ABC transporter 1
Domain1807 – 2039233ABC transporter 2
Nucleotide binding838 – 8458ATP 1 Potential
Nucleotide binding1841 – 18488ATP 2 Potential

Amino acid modifications

Glycosylation3091N-linked (GlcNAc...) Potential
Disulfide bond75 ↔ 222 By similarity
Disulfide bond1359 ↔ 1373 By similarity

Experimental info

Sequence conflict19441N → D in AAF31434. Ref.2
Sequence conflict20041A → R in AAF31434. Ref.2
Sequence conflict20691L → M in AAF31434. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q91V24 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: CD2BE3FE0D8B822B

FASTA2,159236,884
        10         20         30         40         50         60 
MALGTQLMLL LWKNYTYRRR QPIQLLVELL WPLFLFFILV AVRHSHPPLE HHECHFPNKP 

        70         80         90        100        110        120 
LPSAGTVPWL QGLVCNVNNS CFQHPTPGEK PGVLSNFKDS LISRLLADTR TVLGGHSIQD 

       130        140        150        160        170        180 
MLDALGKLIP VLRAVGGGAR PQESDQPTSQ GSVTKLLEKI LQRASLDPVL GQAQDSMRKF 

       190        200        210        220        230        240 
SDAIRDLAQE LLTLPSLMEL RALLRRPRGS AGSLELVSEA LCSTKGPSSP GGLSLNWYEA 

       250        260        270        280        290        300 
NQLNEFMGPE VAPALPDNSL SPACSEFVGT LDDHPVSRLL WRRLKPLILG KILFAPDTNF 

       310        320        330        340        350        360 
TRKLMAQVNQ TFEELALLRD LHELWGVLGP QIFNFMNDST NVAMLQRLLD VGGTGQRQQT 

       370        380        390        400        410        420 
PRAQKKLEAI KDFLDPSRGG YSWREAHADM GRLAGILGQM MECVSLDKLE AVPSEEALVS 

       430        440        450        460        470        480 
RALELLGERR LWAGIVFLSP EHPLDPSELS SPALSPGHLR FKIRMDIDDV TRTNKIRDKF 

       490        500        510        520        530        540 
WDPGPSADPF MDLRYVWGGF VYLQDLLEQA AVRVLGGGNS RTGLYLQQMP HPCYVDDVFL 

       550        560        570        580        590        600 
RVLSRSLPLF LTLAWIYSVA LTVKAVVREK ETRLRETMRA MGLSRAVLWL GWFLSCLGPF 

       610        620        630        640        650        660 
LVSAALLVLV LKLGNILPYS HPVVIFLFLA AFAVATVAQS FLLSAFFSRA NLAAACGGLA 

       670        680        690        700        710        720 
YFALYLPYVL CVAWRERLHL GGLLAASLLS PVAFGFGCES LALLEEQGDG AQWHNLGTGP 

       730        740        750        760        770        780 
AEDVFSLAQV SAFLLLDAVI YGLALWYLEA VCPGQYGIPE PWNFPFRRSY WCGPGPPKSS 

       790        800        810        820        830        840 
VLAPAPQDPK VLVEEPPLGL VPGVSIRGLK KHFRGCPQPA LQGLNLDFYE GHITAFLGHN 

       850        860        870        880        890        900 
GAGKTTTLSI LSGLFPPSSG SASILGHDVQ TNMAAIRPHL GICPQYNVLF DMLTVEEHVW 

       910        920        930        940        950        960 
FYGRLKGVSA AAMGPERERL IRDVGLTLKR DTQTRHLSGG MQRKLSVAIA FVGGSRVVIM 

       970        980        990       1000       1010       1020 
DEPTAGVDPA SRRGIWELLL KYREGRTLIL STHHLDEAEL LGDRVAMVAG GSLCCCGSPL 

      1030       1040       1050       1060       1070       1080 
FLRRHLGCGY YLTLVKSSQS LVTHDAKGDS EDPRREKKSD GNGRTSDTAF TRGTSDKSNQ 

      1090       1100       1110       1120       1130       1140 
APAPGAVPIT PSTARILELV QQHVPGAQLV EDLPHELLLV LPYAGALDGS FAMVFQELDQ 

      1150       1160       1170       1180       1190       1200 
QLELLGLTGY GISDTNLEEI FLKVVEDAHR EGGDSRPQLH LRTCTPQPPT GPEASVLENG 

      1210       1220       1230       1240       1250       1260 
ELAPQGLAPN AAQVQGWTLT CQQLRALLHK RFLLARRSRR GLFAQVVLPA LFVGLALFFS 

      1270       1280       1290       1300       1310       1320 
LIVPPFGQYP PLQLSPAMYG PQVSFFSEDA PGDPNRMKLL EALLGEAGLQ EPSMQDKDAR 

      1330       1340       1350       1360       1370       1380 
GSECTHSLAC YFTVPEVPPD VASILASGNW TPESPSPACQ CSQPGARRLL PDCPAGAGGP 

      1390       1400       1410       1420       1430       1440 
PPPQAVAGLG EVVQNLTGRN VSDFLVKTYP SLVRRGLKTK KWVDEVRYGG FSLGGRDPDL 

      1450       1460       1470       1480       1490       1500 
PTGHEVVRTL AEIRALLSPQ PGNALDRILN NLTQWALGLD ARNSLKIWFN NKGWHAMVAF 

      1510       1520       1530       1540       1550       1560 
VNRANNGLLH ALLPSGPVRH AHSITTLNHP LNLTKEQLSE ATLIASSVDV LVSICVVFAM 

      1570       1580       1590       1600       1610       1620 
SFVPASFTLV LIEERITRAK HLQLVSGLPQ TLYWLGNFLW DMCNYLVAVC IVVFIFLAFQ 

      1630       1640       1650       1660       1670       1680 
QRAYVAPENL PALLLLLLLY GWSITPLMYP ASFFFSVPST AYVVLTCINL FIGINSSMAT 

      1690       1700       1710       1720       1730       1740 
FVLELLSDQN LQEVSRILKQ VFLIFPHFCL GRGLIDMVRN QAMADAFERL GDKQFQSPLR 

      1750       1760       1770       1780       1790       1800 
WDIIGKNLLA MMAQGPLFLL ITLLLQHRNR LLPQSKPRLL PPLGEEDEDV AQERERVTKG 

      1810       1820       1830       1840       1850       1860 
ATQGDVLVLR DLTKVYRGQR NPAVDRLCLG IPPGECFGLL GVNGAGKTST FRMVTGDTLP 

      1870       1880       1890       1900       1910       1920 
SSGEAVLAGH NVAQERSAAH RSMGYCPQSD AIFDLLTGRE HLELFARLRG VPEAQVAQTA 

      1930       1940       1950       1960       1970       1980 
LSGLVRLGLP SYADRPAGTY SGGNKRKLAT ALALVGDPAV VFLDEPTTGM DPSARRFLWN 

      1990       2000       2010       2020       2030       2040 
SLLSVVREGR SVVLTSHSME ECEALCTRLA IMVNGRFRCL GSSQHLKGRF GAGHTLTLRV 

      2050       2060       2070       2080       2090       2100 
PPDQPEPAIA FIRITFPGAE LREVHGSRLR FQLPPGGRCT LTRVFRELAA QGRAHGVEDF 

      2110       2120       2130       2140       2150 
SVSQTTLEEV FLYFSKDQGE EEESSRQEAE EEEVSKPGRQ HPKRVSRFLE DPSSVETMI 

« Hide

References

« Hide 'large scale' references
[1]"Comparative analysis of the promoter structure and genomic organization of the human and mouse ABCA7 gene encoding a novel ABCA transporter."
Broccardo C., Osorio J., Luciani M.-F., Schriml L.M., Prades C., Shulenin S., Arnould I., Naudin L., Lafargue C., Rosier M., Jordan B., Mattei M.-G., Dean M., Denefle P., Chimini G.
Cytogenet. Cell Genet. 92:264-270(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: DBA/2.
Tissue: Lymphoma.
[2]"Identification of 18 mouse ABC genes and characterization of the ABC superfamily in Mus musculus."
Schriml L.M., Dean M.
Genomics 64:24-31(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1920-2159.
Strain: C57BL/6J.
[3]"ATP-binding cassette transporter A7 (ABCA7) binds apolipoprotein A-I and mediates cellular phospholipid but not cholesterol efflux."
Wang N., Lan D., Gerbod-Giannone M., Linsel-Nitschke P., Jehle A.W., Chen W., Martinez L.O., Tall A.R.
J. Biol. Chem. 278:42906-42912(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[4]"Acute digoxin loading reduces ABCA8A mRNA expression in the mouse liver."
Wakaumi M., Ishibashi K., Ando H., Kasanuki H., Tsuruoka S.
Clin. Exp. Pharmacol. Physiol. 32:1034-1041(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[5]"Abca7 null mice retain normal macrophage phosphatidylcholine and cholesterol efflux activity despite alterations in adipose mass and serum cholesterol levels."
Kim W.S., Fitzgerald M.L., Kang K., Okuhira K., Bell S.A., Manning J.J., Koehn S.L., Lu N., Moore K.J., Freeman M.W.
J. Biol. Chem. 280:3989-3995(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]"ATP-binding cassette transporter A7 enhances phagocytosis of apoptotic cells and associated ERK signaling in macrophages."
Jehle A.W., Gardai S.J., Li S., Linsel-Nitschke P., Morimoto K., Janssen W.J., Vandivier R.W., Wang N., Greenberg S., Dale B.M., Qin C., Henson P.M., Tall A.R.
J. Cell Biol. 174:547-556(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[7]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF287141 mRNA. Translation: AAK56862.1.
AF287142 Genomic DNA. Translation: AAK56863.1.
AF213395 mRNA. Translation: AAF31434.1. Frameshift.
CCDSCCDS24004.1.
RefSeqNP_038878.1. NM_013850.1.
UniGeneMm.103351.

3D structure databases

ProteinModelPortalQ91V24.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ91V24.

Proteomic databases

PaxDbQ91V24.
PRIDEQ91V24.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043866; ENSMUSP00000043090; ENSMUSG00000035722.
ENSMUST00000132517; ENSMUSP00000115111; ENSMUSG00000035722.
GeneID27403.
KEGGmmu:27403.
UCSCuc007gba.1. mouse.

Organism-specific databases

CTD10347.
MGIMGI:1351646. Abca7.

Phylogenomic databases

eggNOGCOG1131.
GeneTreeENSGT00720000108414.
HOVERGENHBG050436.
InParanoidQ91V24.
KOK05645.
OrthoDBEOG78D7J6.

Gene expression databases

ArrayExpressQ91V24.
BgeeQ91V24.
GenevestigatorQ91V24.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR026082. ABC_A.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR19229. PTHR19229. 1 hit.
PfamPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio305402.
PROQ91V24.
SOURCESearch...

Entry information

Entry nameABCA7_MOUSE
AccessionPrimary (citable) accession number: Q91V24
Secondary accession number(s): Q9JL36
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot