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Q91RA6

- LMBD2_REOVL

UniProt

Q91RA6 - LMBD2_REOVL

Protein

Outer capsid protein lambda-2

Gene

L2

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei190 – 1901Involved in formation of the phosphoamide bondBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi893 – 9008ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. GTP binding Source: UniProtKB-KW
    3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    4. mRNA guanylyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    ATP-binding, GTP-binding, Nucleotide-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein lambda-2
    Short name:
    Lambda2
    Alternative name(s):
    Lambda2(Cap)
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    Gene namesi
    Name:L2
    OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
    Taxonomic identifieri10884 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000007253: Genome

    Subcellular locationi

    Virion Curated

    GO - Cellular componenti

    1. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12891289Outer capsid protein lambda-2PRO_0000345000Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with protein mu-NS; in viral inclusions.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ91RA6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi181 – 1844Poly-Asp
    Compositional biasi894 – 8974Poly-Ala

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.40.50.150. 1 hit.
    InterProiIPR013783. Ig-like_fold.
    IPR010311. Reovirus_L2.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF06016. Reovirus_L2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000845. Reovirus_L2. 1 hit.
    ProDomiPD149122. Reovirus_L2. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Q91RA6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANVWGVRLA DSLSSPTIET RTRHYTLRDF CSDLDAVAGK EPWRPLRNQR     50
    TNDIVAVQLF RPLQGLVLDT QFYGFPGIFS EWEQFIKEKL RVLKYEVLRI 100
    YPISNYNHER VNVFVANALV GAFLSNQAFY DLLPLLVIND TMINDLLGTG 150
    AALSQFFQSH GEVLEVAAGR KYLQMKNYSN DDDDPPLFAK DLSDYAKAFY 200
    SDTFETLDRF FWTHDSSAGV LVHYDKPTNG NHYILGTLTQ MVSAPPHIIN 250
    ATDALLLESC LEQFAANVRA RPAQPVARLD QCYHLRWGAQ YVGEDSLTYR 300
    LGVLSLLATN GYQLARPIPK QLTNRWLSSF VSQVMSDGVN ETPLWPQERY 350
    VQIAYDSPSV VDGATHYGYV RRNQLRLGMR VSALQSLSDT PAPIQWLPQY 400
    TIEQAAVDEG DLMVSRLTQL PLRPDYGSIW VGDALSYYVD YNRSHRVVLS 450
    SELPQLPDTY FDGDEQYGRS LFSLARKIGD RSLIKDTAVL KHAYQAIDPN 500
    TGKEYLRAGQ SVAYFGASAG HSGADQPLVI EPWTQGKISG VPPPSSVRQF 550
    GYDVAKGAIV DLARPFPSGD YQFVYSDVDQ VVDGHDDLSI SSGLVESLLD 600
    SCMHATSPGG SFVMKINFPT RTVWHYIEQK ILPNITSYML IKPFVTNNVE 650
    LFFVAFGVHQ QSALTWTSGV YFFLVDHFYR YETLSTISRQ LPSFGYVDDG 700
    SSVTGIEMIS LENPGFSNMT QAARVGISGL CANVGNARKL ISIHESHGAR 750
    VLTITSRRSP ASARRKARLR YLPLVDPRSL EVQARTILPS NPVLFDNVNG 800
    ASPHVCLTMM YNFEVSSAVY DGDVVLDLGT GPEAKILELI PPTSPVTCVD 850
    IRPTAQPSGC WNVRTTFLEL DYLSDGWITG IRGDIVTCML SLGAAAAGKS 900
    MTFDAAFQQL VKVLTKSTAN VLLIQVNCPT DVIRTIKGYL EIDQTNKRYR 950
    FPKFGRDEPY SDMDSLERIC RAAWPNCSIT WVPLSYDLRW TKLALLESTT 1000
    LSSASVRIAE LMYKYMPVMR IDIHGLPMEK QGNFIVGQNC SLTIPGFNAQ 1050
    DVFNCYFNSA LAFSTEDVNS AMIPQVTAQF DANKGEWSLD MVFSDAGIYT 1100
    MQALVGSNAN PVSLGSFVVD SPDVDITDAW PAQLDFTIAG TDVDITVNPY 1150
    YRLMAFVRID GQWQIANPDK FQFFSSSTGT LVMNVKLDIA DRYLLYYIRD 1200
    VQSRDVGFYI QHPLQLLNTI TLPTNEDLFL SAPDMREWAV KESGNTICIL 1250
    NSQGFVPPQD WDVLTDTISW SPSLPTYVVP PGDYTLTPL 1289
    Length:1,289
    Mass (Da):143,942
    Last modified:December 1, 2001 - v1
    Checksum:i44AC4D567FD8A72E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF378003 mRNA. Translation: AAK57507.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF378003 mRNA. Translation: AAK57507.1 .

    3D structure databases

    ProteinModelPortali Q91RA6.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.40.50.150. 1 hit.
    InterProi IPR013783. Ig-like_fold.
    IPR010311. Reovirus_L2.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF06016. Reovirus_L2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000845. Reovirus_L2. 1 hit.
    ProDomi PD149122. Reovirus_L2. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian reovirus L2 gene and lambda2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing."
      Breun L.A., Broering T.J., McCutcheon A.M., Harrison S.J., Luongo C.L., Nibert M.L.
      Virology 287:333-348(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
      Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
      J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PROTEIN MU-NS.

    Entry informationi

    Entry nameiLMBD2_REOVL
    AccessioniPrimary (citable) accession number: Q91RA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3