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Q91RA6

- LMBD2_REOVL

UniProt

Q91RA6 - LMBD2_REOVL

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Protein
Outer capsid protein lambda-2
Gene
L2
Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei190 – 1901Involved in formation of the phosphoamide bond By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi893 – 9008ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  4. mRNA guanylyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein lambda-2
Short name:
Lambda2
Alternative name(s):
Lambda2(Cap)
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
Gene namesi
Name:L2
OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Taxonomic identifieri10884 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000007253: Genome

Subcellular locationi

Virion Reviewed prediction

GO - Cellular componenti

  1. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12891289Outer capsid protein lambda-2
PRO_0000345000Add
BLAST

Interactioni

Subunit structurei

Interacts with protein mu-NS; in viral inclusions.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ91RA6.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi181 – 1844Poly-Asp
Compositional biasi894 – 8974Poly-Ala

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR010311. Reovirus_L2.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF06016. Reovirus_L2. 1 hit.
[Graphical view]
PIRSFiPIRSF000845. Reovirus_L2. 1 hit.
ProDomiPD149122. Reovirus_L2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q91RA6-1 [UniParc]FASTAAdd to Basket

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MANVWGVRLA DSLSSPTIET RTRHYTLRDF CSDLDAVAGK EPWRPLRNQR     50
TNDIVAVQLF RPLQGLVLDT QFYGFPGIFS EWEQFIKEKL RVLKYEVLRI 100
YPISNYNHER VNVFVANALV GAFLSNQAFY DLLPLLVIND TMINDLLGTG 150
AALSQFFQSH GEVLEVAAGR KYLQMKNYSN DDDDPPLFAK DLSDYAKAFY 200
SDTFETLDRF FWTHDSSAGV LVHYDKPTNG NHYILGTLTQ MVSAPPHIIN 250
ATDALLLESC LEQFAANVRA RPAQPVARLD QCYHLRWGAQ YVGEDSLTYR 300
LGVLSLLATN GYQLARPIPK QLTNRWLSSF VSQVMSDGVN ETPLWPQERY 350
VQIAYDSPSV VDGATHYGYV RRNQLRLGMR VSALQSLSDT PAPIQWLPQY 400
TIEQAAVDEG DLMVSRLTQL PLRPDYGSIW VGDALSYYVD YNRSHRVVLS 450
SELPQLPDTY FDGDEQYGRS LFSLARKIGD RSLIKDTAVL KHAYQAIDPN 500
TGKEYLRAGQ SVAYFGASAG HSGADQPLVI EPWTQGKISG VPPPSSVRQF 550
GYDVAKGAIV DLARPFPSGD YQFVYSDVDQ VVDGHDDLSI SSGLVESLLD 600
SCMHATSPGG SFVMKINFPT RTVWHYIEQK ILPNITSYML IKPFVTNNVE 650
LFFVAFGVHQ QSALTWTSGV YFFLVDHFYR YETLSTISRQ LPSFGYVDDG 700
SSVTGIEMIS LENPGFSNMT QAARVGISGL CANVGNARKL ISIHESHGAR 750
VLTITSRRSP ASARRKARLR YLPLVDPRSL EVQARTILPS NPVLFDNVNG 800
ASPHVCLTMM YNFEVSSAVY DGDVVLDLGT GPEAKILELI PPTSPVTCVD 850
IRPTAQPSGC WNVRTTFLEL DYLSDGWITG IRGDIVTCML SLGAAAAGKS 900
MTFDAAFQQL VKVLTKSTAN VLLIQVNCPT DVIRTIKGYL EIDQTNKRYR 950
FPKFGRDEPY SDMDSLERIC RAAWPNCSIT WVPLSYDLRW TKLALLESTT 1000
LSSASVRIAE LMYKYMPVMR IDIHGLPMEK QGNFIVGQNC SLTIPGFNAQ 1050
DVFNCYFNSA LAFSTEDVNS AMIPQVTAQF DANKGEWSLD MVFSDAGIYT 1100
MQALVGSNAN PVSLGSFVVD SPDVDITDAW PAQLDFTIAG TDVDITVNPY 1150
YRLMAFVRID GQWQIANPDK FQFFSSSTGT LVMNVKLDIA DRYLLYYIRD 1200
VQSRDVGFYI QHPLQLLNTI TLPTNEDLFL SAPDMREWAV KESGNTICIL 1250
NSQGFVPPQD WDVLTDTISW SPSLPTYVVP PGDYTLTPL 1289
Length:1,289
Mass (Da):143,942
Last modified:December 1, 2001 - v1
Checksum:i44AC4D567FD8A72E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF378003 mRNA. Translation: AAK57507.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF378003 mRNA. Translation: AAK57507.1 .

3D structure databases

ProteinModelPortali Q91RA6.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.40.50.150. 1 hit.
InterProi IPR013783. Ig-like_fold.
IPR010311. Reovirus_L2.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF06016. Reovirus_L2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000845. Reovirus_L2. 1 hit.
ProDomi PD149122. Reovirus_L2. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Mammalian reovirus L2 gene and lambda2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing."
    Breun L.A., Broering T.J., McCutcheon A.M., Harrison S.J., Luongo C.L., Nibert M.L.
    Virology 287:333-348(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
    Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
    J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PROTEIN MU-NS.

Entry informationi

Entry nameiLMBD2_REOVL
AccessioniPrimary (citable) accession number: Q91RA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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