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Q91RA6 (LMBD2_REOVL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein lambda-2

Short name=Lambda2
Alternative name(s):
Lambda2(Cap)

Including the following 2 domains:

  1. mRNA guanylyltransferase
    EC=2.7.7.50
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
Gene names
Name:L2
OrganismReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]
Taxonomic identifier10884 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length1289 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities By similarity.

Catalytic activity

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subunit structure

Interacts with protein mu-NS; in viral inclusions. Ref.2

Subcellular location

Virion Potential.

Sequence similarities

Belongs to the orthoreovirus lambda-2 protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12891289Outer capsid protein lambda-2
PRO_0000345000

Regions

Nucleotide binding893 – 9008ATP Potential
Compositional bias181 – 1844Poly-Asp
Compositional bias894 – 8974Poly-Ala

Sites

Site1901Involved in formation of the phosphoamide bond By similarity

Sequences

Sequence LengthMass (Da)Tools
Q91RA6 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 44AC4D567FD8A72E

FASTA1,289143,942
        10         20         30         40         50         60 
MANVWGVRLA DSLSSPTIET RTRHYTLRDF CSDLDAVAGK EPWRPLRNQR TNDIVAVQLF 

        70         80         90        100        110        120 
RPLQGLVLDT QFYGFPGIFS EWEQFIKEKL RVLKYEVLRI YPISNYNHER VNVFVANALV 

       130        140        150        160        170        180 
GAFLSNQAFY DLLPLLVIND TMINDLLGTG AALSQFFQSH GEVLEVAAGR KYLQMKNYSN 

       190        200        210        220        230        240 
DDDDPPLFAK DLSDYAKAFY SDTFETLDRF FWTHDSSAGV LVHYDKPTNG NHYILGTLTQ 

       250        260        270        280        290        300 
MVSAPPHIIN ATDALLLESC LEQFAANVRA RPAQPVARLD QCYHLRWGAQ YVGEDSLTYR 

       310        320        330        340        350        360 
LGVLSLLATN GYQLARPIPK QLTNRWLSSF VSQVMSDGVN ETPLWPQERY VQIAYDSPSV 

       370        380        390        400        410        420 
VDGATHYGYV RRNQLRLGMR VSALQSLSDT PAPIQWLPQY TIEQAAVDEG DLMVSRLTQL 

       430        440        450        460        470        480 
PLRPDYGSIW VGDALSYYVD YNRSHRVVLS SELPQLPDTY FDGDEQYGRS LFSLARKIGD 

       490        500        510        520        530        540 
RSLIKDTAVL KHAYQAIDPN TGKEYLRAGQ SVAYFGASAG HSGADQPLVI EPWTQGKISG 

       550        560        570        580        590        600 
VPPPSSVRQF GYDVAKGAIV DLARPFPSGD YQFVYSDVDQ VVDGHDDLSI SSGLVESLLD 

       610        620        630        640        650        660 
SCMHATSPGG SFVMKINFPT RTVWHYIEQK ILPNITSYML IKPFVTNNVE LFFVAFGVHQ 

       670        680        690        700        710        720 
QSALTWTSGV YFFLVDHFYR YETLSTISRQ LPSFGYVDDG SSVTGIEMIS LENPGFSNMT 

       730        740        750        760        770        780 
QAARVGISGL CANVGNARKL ISIHESHGAR VLTITSRRSP ASARRKARLR YLPLVDPRSL 

       790        800        810        820        830        840 
EVQARTILPS NPVLFDNVNG ASPHVCLTMM YNFEVSSAVY DGDVVLDLGT GPEAKILELI 

       850        860        870        880        890        900 
PPTSPVTCVD IRPTAQPSGC WNVRTTFLEL DYLSDGWITG IRGDIVTCML SLGAAAAGKS 

       910        920        930        940        950        960 
MTFDAAFQQL VKVLTKSTAN VLLIQVNCPT DVIRTIKGYL EIDQTNKRYR FPKFGRDEPY 

       970        980        990       1000       1010       1020 
SDMDSLERIC RAAWPNCSIT WVPLSYDLRW TKLALLESTT LSSASVRIAE LMYKYMPVMR 

      1030       1040       1050       1060       1070       1080 
IDIHGLPMEK QGNFIVGQNC SLTIPGFNAQ DVFNCYFNSA LAFSTEDVNS AMIPQVTAQF 

      1090       1100       1110       1120       1130       1140 
DANKGEWSLD MVFSDAGIYT MQALVGSNAN PVSLGSFVVD SPDVDITDAW PAQLDFTIAG 

      1150       1160       1170       1180       1190       1200 
TDVDITVNPY YRLMAFVRID GQWQIANPDK FQFFSSSTGT LVMNVKLDIA DRYLLYYIRD 

      1210       1220       1230       1240       1250       1260 
VQSRDVGFYI QHPLQLLNTI TLPTNEDLFL SAPDMREWAV KESGNTICIL NSQGFVPPQD 

      1270       1280 
WDVLTDTISW SPSLPTYVVP PGDYTLTPL 

« Hide

References

[1]"Mammalian reovirus L2 gene and lambda2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing."
Breun L.A., Broering T.J., McCutcheon A.M., Harrison S.J., Luongo C.L., Nibert M.L.
Virology 287:333-348(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PROTEIN MU-NS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF378003 mRNA. Translation: AAK57507.1.

3D structure databases

ProteinModelPortalQ91RA6.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR013783. Ig-like_fold.
IPR010311. Reovirus_L2.
[Graphical view]
PfamPF06016. Reovirus_L2. 1 hit.
[Graphical view]
PIRSFPIRSF000845. Reovirus_L2. 1 hit.
ProDomPD149122. Reovirus_L2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameLMBD2_REOVL
AccessionPrimary (citable) accession number: Q91RA6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families