Q91RA4 (LMBD2_REOVJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer capsid protein lambda-2
Short name=Lambda2

Alternative name(s):
Lambda2(Cap)

Including the following 2 domains:

1. mRNA guanylyltransferase
   EC=2.7.7.50
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56

Gene names

Name:

L2

Organism

Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2) [Complete proteome]

Taxonomic identifier

10885 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	1288 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with protein mu-NS; in viral inclusions By similarity.
Subcellular location	Virion Potential.
Sequence similarities	Belongs to the orthoreovirus lambda-2 protein family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Virion
Ligand	ATP-binding GTP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Capsid protein Methyltransferase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1288	1288	Outer capsid protein lambda-2		PRO_0000344999
Regions
	Nucleotide binding	892 – 899	8	ATP Potential
	Compositional bias	181 – 184	4	Poly-Asp
	Compositional bias	893 – 896	4	Poly-Ala
Sites
	Site	190	1	Involved in formation of the phosphoamide bond By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91RA4 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DD4BC3C1488AA137	FASTA	1,288	143,152
	10 20 30 40 50 60 MANVWGVRLA DSLSSPTLES RNRSYTLHDF CSDLDASAGK EPWKALRNQR TSEIVAVRLF 70 80 90 100 110 120 RPLQGLILDT HMYGFPGEFD AWEVFVKEKL RVLKYEVLRV YPISGYSNSH VNVFVANALV 130 140 150 160 170 180 GAFLSNQAFY DLLPLLIIND TMINDLLGAG VSLAQFFQAH GDVLEVAAGR KYIQMNGYSN 190 200 210 220 230 240 DDDDPPLFAK DLSDYAKAFY CESFEVLDRF FWTHDASAGV LVHYDKPTNG NHYLLGTLTQ 250 260 270 280 290 300 MVSAPPFIIN ATDAMMLESC VEQFAANAAA RPAQPATRLD QCYHLRWGAQ YVGEDSLTYR 310 320 330 340 350 360 LGVLSLLATN GYQLARPIPK QLTNRWLSSF VSQIMSEGAN ETPLWPQERY VQIAYDSPSV 370 380 390 400 410 420 VDGAVQYGYV RKNQLRLGMR ISPIQSLSDV PAPVAWLPQY TIDQTALEDG DMVGHMSQLP 430 440 450 460 470 480 LRPEYGSMWV GEALSYYVDY NQSHRVVAAK ELPQLPDTYF DGDEQYGRSL FSLARRIGDR 490 500 510 520 530 540 SLIKDTAVLK HAYQAIDPST GREYLRAGQS VAYFGASAGH SGADQPLVIE PWLQGKISGV 550 560 570 580 590 600 PSPASIRQFG YDVAKGAIVD LARPFPSGDY QFVYSDVDQV VDGHDDLSIS SNLVESILSS 610 620 630 640 650 660 CMQATSPGGS FVAKINFPTR SIWYYIEQKI LPNITSYMII KPFVTNNVEV FFVAFGVHRQ 670 680 690 700 710 720 SSLTWTSGVY FFLVDHFYRY ETLSAISRQL PSYGYVDDGS SVTGLEVISI ENPGFSTMTQ 730 740 750 760 770 780 ASRVAISALC ANTGNSRKTI SIYESHGARV LMLVSRRSPA SAKRKARLRY LPLIDPRSLE 790 800 810 820 830 840 VQSRTIMPST PVLFENSNGA SPHVCLTMMY NYEVSSAVYD GDVVLDLGTG PEAKILELIP 850 860 870 880 890 900 PTSPATCVDI RPTAQPTGCW NVRTTFLQLD YLSDGWITGV RGDIVTCMLS LGAAAAGKSM 910 920 930 940 950 960 TFDAAFQQFV RVIAQSAANV VLVQVNCPTD VIRSVRGYLE IDQTSKRYRF PKFGRDEPYS 970 980 990 1000 1010 1020 DMESLERICR ATWPNCSITW VPLSYDLRWT RLALLEAATL NSASIRIAEL MYKYMPVMRV 1030 1040 1050 1060 1070 1080 DIHGLPMNKS GNFVVGQNCS LTIPGFNAQD TFNCYYNSAL AFSTEDVNAA MIPSVTATFD 1090 1100 1110 1120 1130 1140 NAKNEWTLDM VFSDAGIYTM QAVVGVNASP IALGSFVVDS PDVDITDAWP AQLDFTIAGT 1150 1160 1170 1180 1190 1200 DVDITVNPYY RLMAFVKIDG QWQIANPDKF QFFASATGTL TMNVKLDIAD KYLLYYIRDV 1210 1220 1230 1240 1250 1260 QSREVGFYIQ HPLQLLNTIT LPTNEDLFLS APDMREWAVK ESGNTICILN SQGFIPPQDW 1270 1280 DVLTDTISWS PSLPTYVVPP GDYTLTPL

References

[1]	"Mammalian reovirus L2 gene and lambda2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing." Breun L.A., Broering T.J., McCutcheon A.M., Harrison S.J., Luongo C.L., Nibert M.L. Virology 287:333-348(2001) [PubMed: 11531411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF378005 mRNA. Translation: AAK57509.1.
3D structure databases
HSSP	HSSP built from PDB template 1EJ6 based on UniProtKB P11079.
ProteinModelPortal	Q91RA4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010311. Reovirus_L2. [Graphical view]
Pfam	PF06016. Reovirus_L2. 1 hit. [Graphical view]
PIRSF	PIRSF000845. Reovirus_L2. 1 hit.
ProDom	PD149122. Reovirus_L2. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

LMBD2_REOVJ

Accession

Primary (citable) accession number: Q91RA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	December 1, 2001
Last modified:	May 3, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program