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Q91RA4

- LMBD2_REOVJ

UniProt

Q91RA4 - LMBD2_REOVJ

Protein

Outer capsid protein lambda-2

Gene

L2

Organism
Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei190 – 1901Involved in formation of the phosphoamide bondBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi892 – 8998ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. GTP binding Source: UniProtKB-KW
    3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    4. mRNA guanylyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    ATP-binding, GTP-binding, Nucleotide-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein lambda-2
    Short name:
    Lambda2
    Alternative name(s):
    Lambda2(Cap)
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    Gene namesi
    Name:L2
    OrganismiReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
    Taxonomic identifieri10885 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000006370: Genome

    Subcellular locationi

    Virion Curated

    GO - Cellular componenti

    1. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12881288Outer capsid protein lambda-2PRO_0000344999Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with protein mu-NS; in viral inclusions.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ91RA4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi181 – 1844Poly-Asp
    Compositional biasi893 – 8964Poly-Ala

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.40.50.150. 1 hit.
    InterProiIPR013783. Ig-like_fold.
    IPR010311. Reovirus_L2.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF06016. Reovirus_L2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000845. Reovirus_L2. 1 hit.
    ProDomiPD149122. Reovirus_L2. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Q91RA4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANVWGVRLA DSLSSPTLES RNRSYTLHDF CSDLDASAGK EPWKALRNQR     50
    TSEIVAVRLF RPLQGLILDT HMYGFPGEFD AWEVFVKEKL RVLKYEVLRV 100
    YPISGYSNSH VNVFVANALV GAFLSNQAFY DLLPLLIIND TMINDLLGAG 150
    VSLAQFFQAH GDVLEVAAGR KYIQMNGYSN DDDDPPLFAK DLSDYAKAFY 200
    CESFEVLDRF FWTHDASAGV LVHYDKPTNG NHYLLGTLTQ MVSAPPFIIN 250
    ATDAMMLESC VEQFAANAAA RPAQPATRLD QCYHLRWGAQ YVGEDSLTYR 300
    LGVLSLLATN GYQLARPIPK QLTNRWLSSF VSQIMSEGAN ETPLWPQERY 350
    VQIAYDSPSV VDGAVQYGYV RKNQLRLGMR ISPIQSLSDV PAPVAWLPQY 400
    TIDQTALEDG DMVGHMSQLP LRPEYGSMWV GEALSYYVDY NQSHRVVAAK 450
    ELPQLPDTYF DGDEQYGRSL FSLARRIGDR SLIKDTAVLK HAYQAIDPST 500
    GREYLRAGQS VAYFGASAGH SGADQPLVIE PWLQGKISGV PSPASIRQFG 550
    YDVAKGAIVD LARPFPSGDY QFVYSDVDQV VDGHDDLSIS SNLVESILSS 600
    CMQATSPGGS FVAKINFPTR SIWYYIEQKI LPNITSYMII KPFVTNNVEV 650
    FFVAFGVHRQ SSLTWTSGVY FFLVDHFYRY ETLSAISRQL PSYGYVDDGS 700
    SVTGLEVISI ENPGFSTMTQ ASRVAISALC ANTGNSRKTI SIYESHGARV 750
    LMLVSRRSPA SAKRKARLRY LPLIDPRSLE VQSRTIMPST PVLFENSNGA 800
    SPHVCLTMMY NYEVSSAVYD GDVVLDLGTG PEAKILELIP PTSPATCVDI 850
    RPTAQPTGCW NVRTTFLQLD YLSDGWITGV RGDIVTCMLS LGAAAAGKSM 900
    TFDAAFQQFV RVIAQSAANV VLVQVNCPTD VIRSVRGYLE IDQTSKRYRF 950
    PKFGRDEPYS DMESLERICR ATWPNCSITW VPLSYDLRWT RLALLEAATL 1000
    NSASIRIAEL MYKYMPVMRV DIHGLPMNKS GNFVVGQNCS LTIPGFNAQD 1050
    TFNCYYNSAL AFSTEDVNAA MIPSVTATFD NAKNEWTLDM VFSDAGIYTM 1100
    QAVVGVNASP IALGSFVVDS PDVDITDAWP AQLDFTIAGT DVDITVNPYY 1150
    RLMAFVKIDG QWQIANPDKF QFFASATGTL TMNVKLDIAD KYLLYYIRDV 1200
    QSREVGFYIQ HPLQLLNTIT LPTNEDLFLS APDMREWAVK ESGNTICILN 1250
    SQGFIPPQDW DVLTDTISWS PSLPTYVVPP GDYTLTPL 1288
    Length:1,288
    Mass (Da):143,152
    Last modified:December 1, 2001 - v1
    Checksum:iDD4BC3C1488AA137
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF378005 mRNA. Translation: AAK57509.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF378005 mRNA. Translation: AAK57509.1 .

    3D structure databases

    ProteinModelPortali Q91RA4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.40.50.150. 1 hit.
    InterProi IPR013783. Ig-like_fold.
    IPR010311. Reovirus_L2.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF06016. Reovirus_L2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000845. Reovirus_L2. 1 hit.
    ProDomi PD149122. Reovirus_L2. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian reovirus L2 gene and lambda2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing."
      Breun L.A., Broering T.J., McCutcheon A.M., Harrison S.J., Luongo C.L., Nibert M.L.
      Virology 287:333-348(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiLMBD2_REOVJ
    AccessioniPrimary (citable) accession number: Q91RA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3