Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q91RA4 (LMBD2_REOVJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein lambda-2

Short name=Lambda2
Alternative name(s):
Lambda2(Cap)

Including the following 2 domains:

  1. mRNA guanylyltransferase
    EC=2.7.7.50
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
Gene names
Name:L2
OrganismReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2) [Complete proteome]
Taxonomic identifier10885 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length1288 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities By similarity.

Catalytic activity

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subunit structure

Interacts with protein mu-NS; in viral inclusions By similarity.

Subcellular location

Virion Potential.

Sequence similarities

Belongs to the orthoreovirus lambda-2 protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12881288Outer capsid protein lambda-2
PRO_0000344999

Regions

Nucleotide binding892 – 8998ATP Potential
Compositional bias181 – 1844Poly-Asp
Compositional bias893 – 8964Poly-Ala

Sites

Site1901Involved in formation of the phosphoamide bond By similarity

Sequences

Sequence LengthMass (Da)Tools
Q91RA4 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: DD4BC3C1488AA137

FASTA1,288143,152
        10         20         30         40         50         60 
MANVWGVRLA DSLSSPTLES RNRSYTLHDF CSDLDASAGK EPWKALRNQR TSEIVAVRLF 

        70         80         90        100        110        120 
RPLQGLILDT HMYGFPGEFD AWEVFVKEKL RVLKYEVLRV YPISGYSNSH VNVFVANALV 

       130        140        150        160        170        180 
GAFLSNQAFY DLLPLLIIND TMINDLLGAG VSLAQFFQAH GDVLEVAAGR KYIQMNGYSN 

       190        200        210        220        230        240 
DDDDPPLFAK DLSDYAKAFY CESFEVLDRF FWTHDASAGV LVHYDKPTNG NHYLLGTLTQ 

       250        260        270        280        290        300 
MVSAPPFIIN ATDAMMLESC VEQFAANAAA RPAQPATRLD QCYHLRWGAQ YVGEDSLTYR 

       310        320        330        340        350        360 
LGVLSLLATN GYQLARPIPK QLTNRWLSSF VSQIMSEGAN ETPLWPQERY VQIAYDSPSV 

       370        380        390        400        410        420 
VDGAVQYGYV RKNQLRLGMR ISPIQSLSDV PAPVAWLPQY TIDQTALEDG DMVGHMSQLP 

       430        440        450        460        470        480 
LRPEYGSMWV GEALSYYVDY NQSHRVVAAK ELPQLPDTYF DGDEQYGRSL FSLARRIGDR 

       490        500        510        520        530        540 
SLIKDTAVLK HAYQAIDPST GREYLRAGQS VAYFGASAGH SGADQPLVIE PWLQGKISGV 

       550        560        570        580        590        600 
PSPASIRQFG YDVAKGAIVD LARPFPSGDY QFVYSDVDQV VDGHDDLSIS SNLVESILSS 

       610        620        630        640        650        660 
CMQATSPGGS FVAKINFPTR SIWYYIEQKI LPNITSYMII KPFVTNNVEV FFVAFGVHRQ 

       670        680        690        700        710        720 
SSLTWTSGVY FFLVDHFYRY ETLSAISRQL PSYGYVDDGS SVTGLEVISI ENPGFSTMTQ 

       730        740        750        760        770        780 
ASRVAISALC ANTGNSRKTI SIYESHGARV LMLVSRRSPA SAKRKARLRY LPLIDPRSLE 

       790        800        810        820        830        840 
VQSRTIMPST PVLFENSNGA SPHVCLTMMY NYEVSSAVYD GDVVLDLGTG PEAKILELIP 

       850        860        870        880        890        900 
PTSPATCVDI RPTAQPTGCW NVRTTFLQLD YLSDGWITGV RGDIVTCMLS LGAAAAGKSM 

       910        920        930        940        950        960 
TFDAAFQQFV RVIAQSAANV VLVQVNCPTD VIRSVRGYLE IDQTSKRYRF PKFGRDEPYS 

       970        980        990       1000       1010       1020 
DMESLERICR ATWPNCSITW VPLSYDLRWT RLALLEAATL NSASIRIAEL MYKYMPVMRV 

      1030       1040       1050       1060       1070       1080 
DIHGLPMNKS GNFVVGQNCS LTIPGFNAQD TFNCYYNSAL AFSTEDVNAA MIPSVTATFD 

      1090       1100       1110       1120       1130       1140 
NAKNEWTLDM VFSDAGIYTM QAVVGVNASP IALGSFVVDS PDVDITDAWP AQLDFTIAGT 

      1150       1160       1170       1180       1190       1200 
DVDITVNPYY RLMAFVKIDG QWQIANPDKF QFFASATGTL TMNVKLDIAD KYLLYYIRDV 

      1210       1220       1230       1240       1250       1260 
QSREVGFYIQ HPLQLLNTIT LPTNEDLFLS APDMREWAVK ESGNTICILN SQGFIPPQDW 

      1270       1280 
DVLTDTISWS PSLPTYVVPP GDYTLTPL 

« Hide

References

[1]"Mammalian reovirus L2 gene and lambda2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing."
Breun L.A., Broering T.J., McCutcheon A.M., Harrison S.J., Luongo C.L., Nibert M.L.
Virology 287:333-348(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF378005 mRNA. Translation: AAK57509.1.

3D structure databases

ProteinModelPortalQ91RA4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR013783. Ig-like_fold.
IPR010311. Reovirus_L2.
[Graphical view]
PfamPF06016. Reovirus_L2. 1 hit.
[Graphical view]
PIRSFPIRSF000845. Reovirus_L2. 1 hit.
ProDomPD149122. Reovirus_L2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameLMBD2_REOVJ
AccessionPrimary (citable) accession number: Q91RA4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families