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Q91RA4

- LMBD2_REOVJ

UniProt

Q91RA4 - LMBD2_REOVJ

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Protein

Outer capsid protein lambda-2

Gene

L2

Organism
Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities (By similarity).By similarity

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei190 – 1901Involved in formation of the phosphoamide bondBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi892 – 8998ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  4. mRNA guanylyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein lambda-2
Short name:
Lambda2
Alternative name(s):
Lambda2(Cap)
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
Gene namesi
Name:L2
OrganismiReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Taxonomic identifieri10885 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006370: Genome

Subcellular locationi

Virion Curated

GO - Cellular componenti

  1. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12881288Outer capsid protein lambda-2PRO_0000344999Add
BLAST

Interactioni

Subunit structurei

Interacts with protein mu-NS; in viral inclusions.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ91RA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi181 – 1844Poly-Asp
Compositional biasi893 – 8964Poly-Ala

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR010311. Reovirus_L2.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF06016. Reovirus_L2. 1 hit.
[Graphical view]
PIRSFiPIRSF000845. Reovirus_L2. 1 hit.
ProDomiPD149122. Reovirus_L2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q91RA4-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MANVWGVRLA DSLSSPTLES RNRSYTLHDF CSDLDASAGK EPWKALRNQR
60 70 80 90 100
TSEIVAVRLF RPLQGLILDT HMYGFPGEFD AWEVFVKEKL RVLKYEVLRV
110 120 130 140 150
YPISGYSNSH VNVFVANALV GAFLSNQAFY DLLPLLIIND TMINDLLGAG
160 170 180 190 200
VSLAQFFQAH GDVLEVAAGR KYIQMNGYSN DDDDPPLFAK DLSDYAKAFY
210 220 230 240 250
CESFEVLDRF FWTHDASAGV LVHYDKPTNG NHYLLGTLTQ MVSAPPFIIN
260 270 280 290 300
ATDAMMLESC VEQFAANAAA RPAQPATRLD QCYHLRWGAQ YVGEDSLTYR
310 320 330 340 350
LGVLSLLATN GYQLARPIPK QLTNRWLSSF VSQIMSEGAN ETPLWPQERY
360 370 380 390 400
VQIAYDSPSV VDGAVQYGYV RKNQLRLGMR ISPIQSLSDV PAPVAWLPQY
410 420 430 440 450
TIDQTALEDG DMVGHMSQLP LRPEYGSMWV GEALSYYVDY NQSHRVVAAK
460 470 480 490 500
ELPQLPDTYF DGDEQYGRSL FSLARRIGDR SLIKDTAVLK HAYQAIDPST
510 520 530 540 550
GREYLRAGQS VAYFGASAGH SGADQPLVIE PWLQGKISGV PSPASIRQFG
560 570 580 590 600
YDVAKGAIVD LARPFPSGDY QFVYSDVDQV VDGHDDLSIS SNLVESILSS
610 620 630 640 650
CMQATSPGGS FVAKINFPTR SIWYYIEQKI LPNITSYMII KPFVTNNVEV
660 670 680 690 700
FFVAFGVHRQ SSLTWTSGVY FFLVDHFYRY ETLSAISRQL PSYGYVDDGS
710 720 730 740 750
SVTGLEVISI ENPGFSTMTQ ASRVAISALC ANTGNSRKTI SIYESHGARV
760 770 780 790 800
LMLVSRRSPA SAKRKARLRY LPLIDPRSLE VQSRTIMPST PVLFENSNGA
810 820 830 840 850
SPHVCLTMMY NYEVSSAVYD GDVVLDLGTG PEAKILELIP PTSPATCVDI
860 870 880 890 900
RPTAQPTGCW NVRTTFLQLD YLSDGWITGV RGDIVTCMLS LGAAAAGKSM
910 920 930 940 950
TFDAAFQQFV RVIAQSAANV VLVQVNCPTD VIRSVRGYLE IDQTSKRYRF
960 970 980 990 1000
PKFGRDEPYS DMESLERICR ATWPNCSITW VPLSYDLRWT RLALLEAATL
1010 1020 1030 1040 1050
NSASIRIAEL MYKYMPVMRV DIHGLPMNKS GNFVVGQNCS LTIPGFNAQD
1060 1070 1080 1090 1100
TFNCYYNSAL AFSTEDVNAA MIPSVTATFD NAKNEWTLDM VFSDAGIYTM
1110 1120 1130 1140 1150
QAVVGVNASP IALGSFVVDS PDVDITDAWP AQLDFTIAGT DVDITVNPYY
1160 1170 1180 1190 1200
RLMAFVKIDG QWQIANPDKF QFFASATGTL TMNVKLDIAD KYLLYYIRDV
1210 1220 1230 1240 1250
QSREVGFYIQ HPLQLLNTIT LPTNEDLFLS APDMREWAVK ESGNTICILN
1260 1270 1280
SQGFIPPQDW DVLTDTISWS PSLPTYVVPP GDYTLTPL
Length:1,288
Mass (Da):143,152
Last modified:December 1, 2001 - v1
Checksum:iDD4BC3C1488AA137
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378005 mRNA. Translation: AAK57509.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378005 mRNA. Translation: AAK57509.1 .

3D structure databases

ProteinModelPortali Q91RA4.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.40.50.150. 1 hit.
InterProi IPR013783. Ig-like_fold.
IPR010311. Reovirus_L2.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF06016. Reovirus_L2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000845. Reovirus_L2. 1 hit.
ProDomi PD149122. Reovirus_L2. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Mammalian reovirus L2 gene and lambda2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing."
    Breun L.A., Broering T.J., McCutcheon A.M., Harrison S.J., Luongo C.L., Nibert M.L.
    Virology 287:333-348(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiLMBD2_REOVJ
AccessioniPrimary (citable) accession number: Q91RA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 1, 2001
Last modified: October 1, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3