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Protein

RNA replication polyprotein

Gene

ORF1

Organism
Citrus leaf blotch virus (isolate Nagami kumquat/France/SRA-153/1984) (CLBV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

RNA replication polyprotein: RNA-directed RNA polymerase involved in viral RNA replication.By similarity
Protease: Thiol protease that cleaves the polyprotein.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Cofactori

Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi892IronPROSITE-ProRule annotation1
Metal bindingi894IronPROSITE-ProRule annotation1
Metal bindingi946IronPROSITE-ProRule annotation1
Binding sitei9542-oxoglutaratePROSITE-ProRule annotation1
Active sitei982By similarity1
Active sitei1062By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1164 – 1171ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Helicase, Hydrolase, Nucleotidyltransferase, Oxidoreductase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase
Biological processViral RNA replication
LigandATP-binding, Iron, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA replication polyproteinBy similarity
Alternative name(s):
ORF1 proteinBy similarity
Including the following 5 domains:
Viral methyltransferaseSequence analysis (EC:2.1.1.-Curated)
Putative Fe(2+) 2-oxoglutarate dioxygenaseSequence analysis (EC:1.14.11.-PROSITE-ProRule annotation)
ProteaseBy similarity (EC:3.4.22.-By similarity)
RNA-directed RNA polymerasePROSITE-ProRule annotation (EC:2.7.7.48PROSITE-ProRule annotation)
HelicaseBy similarity (EC:3.6.4.13Curated)
Gene namesi
ORF Names:ORF1By similarity
OrganismiCitrus leaf blotch virus (isolate Nagami kumquat/France/SRA-153/1984) (CLBV)
Taxonomic identifieri686981 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageTymoviralesBetaflexiviridaeTrivirinaeCitrivirus
Virus hostiCitrus [TaxID: 2706]
Proteomesi
  • UP000006933 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004010881 – 1962RNA replication polyproteinAdd BLAST1962

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ91QZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 261Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST198
Domaini683 – 794OTUPROSITE-ProRule annotationAdd BLAST112
Domaini874 – 963Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST90
Domaini978 – 1067Peptidase C23PROSITE-ProRule annotationAdd BLAST90
Domaini1122 – 1297(+)RNA virus helicase ATP-bindingAdd BLAST176
Domaini1298 – 1445(+)RNA virus helicase C-terminalAdd BLAST148
Domaini1739 – 1846RdRp catalyticPROSITE-ProRule annotationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi612 – 615Poly-Glu4
Compositional biasi1590 – 1593Poly-Glu4

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiView protein in InterPro
IPR027351. (+)RNA_virus_helicase_core_dom.
IPR037151. AlkB-like_sf.
IPR002588. Alphavirus-like_MT_dom.
IPR003323. OTU_dom.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR027417. P-loop_NTPase.
IPR008041. Peptidase_C23.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
PF05379. Peptidase_C23. 1 hit.
PF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51743. ALPHAVIRUS_MT. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
PS50802. OTU. 1 hit.
PS51492. PEPTIDASE_C23. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91QZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALMSNKTAI ESILGNFEKK HVDAIYNAAA QTILSHSEFR NKHFAYSLNS
60 70 80 90 100
YQKKIASKVG IELYPNGYLP HSHPLSKIFE NHLLFDVLPG VVNTSRLVMC
110 120 130 140 150
SIKESKVLVF KGIRDKSRRQ VSDLNALNSL NNSHTSFINR LVASKDVSRY
160 170 180 190 200
TEEADAFFQS KKGSPELFSR NFIKSLENKE AVFFHDEVHH WTKAQMFSFL
210 220 230 240 250
KSTKVKRFIF TVVYPPEILK KFANSQNPKV YDFKVDKGRL FFFPDGVKTE
260 270 280 290 300
AYEQKLNMEW LFSASHLRSG DCVWTVTRHK SIYAHHLFEI SIGELVTDSK
310 320 330 340 350
LFFSDYNSID MSKIFLDRFR SYEVFPISIE HLYKVYSYLL CLKKPDLESG
360 370 380 390 400
LAKLRQIIGD DVEIKEFLFF EQFCKRLIER QTSWGLFGHS FFEKLTDMAL
410 420 430 440 450
SSLPNSIARI FPQWKKKNTF EFLFSLGTLV VDVERKVCFE HVLEEWGFEV
460 470 480 490 500
VITDENAYLD PLSIFAINEN FNEDRVDDGY LERIRLPFWN LNDYDLKRKR
510 520 530 540 550
VNAYNILSYR FEEERKIESA QKGPNKMLQI EWYGIKEFKV DPFISNSITE
560 570 580 590 600
FTLLEALLGK RIDPKKYSYS KQACTLSNYL TFLCAEGLDG FNLEEHLERR
610 620 630 640 650
LKAAGHDVSD DEEEELTSAE QAGPIKILAD PLGFMKECLE EIPIETEPSL
660 670 680 690 700
EERGQFSTDY HSEKFEINYN DIFNPHNCMN THGDEIPTPS DGNCFFSAFT
710 720 730 740 750
ETFEVERPDT LRSDFSDWLM EFNGGSYASL AEMIRPNGVF MEAELIYLFC
760 770 780 790 800
VFRGVTLIIH DRTHEKENVY AVHRGFEEGH MVHRGNHFVG IETYNISTLT
810 820 830 840 850
SDPLLGDIPC GFSEEITKFH FRPDHFNCAQ FRGRKAAFIT KVDADYGHNG
860 870 880 890 900
MVYPHNSWVP SLEEIIQICG QGDDFNCALI NFYEANSSLG FHRDNERVYN
910 920 930 940 950
DDPILTVCTF GEGRFTIEFK DQVTSFLMTA GSFFLMPKGF QKKARHSVSN
960 970 980 990 1000
EMSRVSITFR KHVRRLNGSP IAIREENYKN TCLINAFSKA MKRSKQAIIA
1010 1020 1030 1040 1050
KLKTVNSPFW SRYLSEGNGG SIEDCQSACE ALDVTVDLNV NGKCVVLGKG
1060 1070 1080 1090 1100
ALRISMALRN NHFSVINAAQ LMERTFVSHL LEKGNVNVLE GFDAMLSGDV
1110 1120 1130 1140 1150
GAAGVNKIQF AANFEFARIL ANSFLNMTTG ICLGKALDNG EKYFLHILKD
1160 1170 1180 1190 1200
RVKQIGIDVT MVCGFAGSGK SRKLQSWLHS RKKGNFCVVS PRTNLAADWA
1210 1220 1230 1240 1250
FKLELEPNEQ RKVSTFEKFI KTDKSKLDLI VIDELTLFPN GYLDLLVYEL
1260 1270 1280 1290 1300
ADVNRHCQII LLFDPLQARY HNKMDESILT FEHDVDRLIG GQNIEYIYST
1310 1320 1330 1340 1350
HRMSRYFNRF FDVPCFNQAD RTEEQRLWIF DDVYSIPSIC SDRQEPCDVL
1360 1370 1380 1390 1400
LVESDLEKKA FSPIINVMTF GESQGLTFNH VCILLSESSA ASNEFRWMVA
1410 1420 1430 1440 1450
LTRARTRFSL CSTFLGGIEE FKVKRKESLI TSILQGEKIT FNRLNLMLKC
1460 1470 1480 1490 1500
NLIRREKENG CRDEVDREER LEGDPFLKPF IFLGQRVEKD EDEVEEVKIR
1510 1520 1530 1540 1550
EPTCQTHLYI TEPNFGLCYN FDFIREKEQR EYREDMLVTN QFCDSYDKVH
1560 1570 1580 1590 1600
INGKRETPGP LRFKAIYPKH SADDDMTFWM AVRKRLVFRE EEENYQRLSR
1610 1620 1630 1640 1650
AHLVGGLLYT NFKKKMGLEF TFDQGLLEES INAFEKKKLE KSCGTIKSHS
1660 1670 1680 1690 1700
IRSDIDWALN DVFLFMKSQL CTKYEKQFVD AKAGQTLACF QHLILVQFAP
1710 1720 1730 1740 1750
WCRYLETQIR NQLPEEIYIH SNKNFDDLNA WVKKFFQRDI CVESDYEAFD
1760 1770 1780 1790 1800
ASQDEYILSF EIHLMKDAHF PQKIIDAYID LKCKLGCKLG HFSIMRFTGE
1810 1820 1830 1840 1850
FCTFLFNTLA NMAFTMCRYE WRRGQPIAFA GDDMCALNNL AVCHDFDDLF
1860 1870 1880 1890 1900
ELISLKAKVE RTETPMFCGW RLTPYGIVKE PELVYNRFQV AIEEGKVLEC
1910 1920 1930 1940 1950
LENYAIEVSY AYSLSERLYE VLKSERQVQY HQAVVRFIVT HIDKLKTKVR
1960
DLFLEQSSDE DI
Length:1,962
Mass (Da):227,379
Last modified:December 1, 2001 - v1
Checksum:i138CC30DD43916D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318061 Genomic RNA. Translation: CAC39422.1.
RefSeqiNP_624333.1. NC_003877.1.

Genome annotation databases

GeneIDi944478.
KEGGivg:944478.

Similar proteinsi

Entry informationi

Entry nameiRDRP_CLBVS
AccessioniPrimary (citable) accession number: Q91QZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: December 1, 2001
Last modified: October 25, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome