ID   PAP1_LSDVN              Reviewed;         474 AA.
AC   Q91MX5; Q77GP9; Q8JTW7;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   08-NOV-2023, entry version 69.
DE   RecName: Full=Poly(A) polymerase catalytic subunit;
DE            EC=2.7.7.19;
DE   AltName: Full=Poly(A) polymerase large subunit;
DE            Short=PAP-L;
GN   Name=PAPL; OrderedLocusNames=LSDV032, LD032, LW032;
OS   Lumpy skin disease virus (isolate Bovine/Kenya/Neethling 2490/1958) (LSDV)
OS   (Lumpy skin disease virus (isolate NI-2490)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Capripoxvirus;
OC   Lumpy skin disease virus.
OX   NCBI_TaxID=376849;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Neethling 2490;
RX   PubMed=11435593; DOI=10.1128/jvi.75.15.7122-7130.2001;
RA   Tulman E.R., Afonso C.L., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
RT   "Genome of lumpy skin disease virus.";
RL   J. Virol. 75:7122-7130(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Neethling vaccine LW 1959, and Isolate Neethling
RC   Warmbaths LW;
RX   PubMed=12827464; DOI=10.1007/s00705-003-0102-0;
RA   Kara P.D., Afonso C.L., Wallace D.B., Kutish G.F., Abolnik C., Lu Z.,
RA   Vreede F.T., Taljaard L.C.F., Zsak A., Viljoen G.J., Rock D.L.;
RT   "Comparative sequence analysis of the South African vaccine strain and two
RT   virulent field isolates of Lumpy skin disease virus.";
RL   Arch. Virol. 148:1335-1356(2003).
CC   -!- FUNCTION: Polymerase that creates the 3'-poly(A) tail of mRNA's.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC         Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC         ChEBI:CHEBI:173115; EC=2.7.7.19;
CC   -!- SUBUNIT: Heterodimer of a large (catalytic) subunit and a small
CC       (regulatory) subunit. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae poly(A) polymerase catalytic
CC       subunit family. {ECO:0000305}.
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DR   EMBL; AF325528; AAK84993.1; -; Genomic_DNA.
DR   EMBL; AF409137; AAN02600.1; -; Genomic_DNA.
DR   EMBL; AF409138; AAN02757.1; -; Genomic_DNA.
DR   RefSeq; NP_150466.1; NC_003027.1.
DR   SMR; Q91MX5; -.
DR   GeneID; 921671; -.
DR   KEGG; vg:921671; -.
DR   OrthoDB; 3428at10239; -.
DR   Proteomes; UP000126568; Segment.
DR   Proteomes; UP000127252; Genome.
DR   Proteomes; UP000156762; Segment.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:1990817; F:poly(A) RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd20919; polyA_pol_Pox; 1.
DR   Gene3D; 1.20.1270.320; Poxvirus poly(A) polymerase, N domain; 1.
DR   Gene3D; 3.30.460.60; Poxvirus poly(A) polymerase, nucleotidyltransferase domain; 1.
DR   InterPro; IPR004976; PolyA_pol_cat_Poxvir.
DR   InterPro; IPR037265; PolyA_pol_cat_sf.
DR   InterPro; IPR024231; PolyA_pol_nucTrfase_Poxvir.
DR   InterPro; IPR038419; PolyA_pol_nucTrfase_sf_Poxvir.
DR   InterPro; IPR024397; Poxvirus_polyA_pol_cat_C.
DR   InterPro; IPR024398; Poxvirus_polyA_pol_cat_N.
DR   InterPro; IPR038337; Poxvirus_polyA_pol_cat_N_sf.
DR   Pfam; PF03296; Pox_polyA_pol; 1.
DR   Pfam; PF12629; Pox_polyA_pol_C; 1.
DR   Pfam; PF12630; Pox_polyA_pol_N; 1.
DR   PIRSF; PIRSF015693; VAC-48L_nuct; 1.
DR   SUPFAM; SSF160957; Poly(A) polymerase catalytic subunit-like; 1.
PE   3: Inferred from homology;
KW   ATP-binding; mRNA processing; Nucleotide-binding; Transcription;
KW   Transferase.
FT   CHAIN           1..474
FT                   /note="Poly(A) polymerase catalytic subunit"
FT                   /id="PRO_0000308934"
FT   ACT_SITE        193
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        195
FT                   /evidence="ECO:0000250"
FT   VARIANT         123
FT                   /note="T -> A (in strain: isolate Neethling vaccine LW
FT                   1959)"
FT   VARIANT         270
FT                   /note="A -> T (in strain: isolate Neethling vaccine LW
FT                   1959)"
SQ   SEQUENCE   474 AA;  54565 MW;  3BD4209BEBDD14F7 CRC64;
     MNDKIVVDVL KLYLKRDPSL TEFHMLKSQY KNIQRVNIFN KEIFISLIKK NKKKFFSDIK
     SSPSDIKRSI LTYFSKQENT YSIGKLYTII ELQSILVTTY TDVLGILTTK GPDLFTSNIY
     YNTSSMQSLA RDALNFMNVA QMTDKTMGRH NVSSLVDNVN SLMEEYLRRH NKNCICYGSY
     SLHLLNPDVK YGDIDILQTN SRTFLIDLAF LIKFITGLNV VLLKVPYLKN YMVLKDQNDS
     HIIDSFNIRQ DTMQSIPKVL IDNIYIVDPA LQLMSMLKMF SQIDRLEDLA KNPEKLTVRL
     ATLLEYVRVN YGINFISNQT KKNNMPMYSV IDIDKRIITV DTSNYDFPFK KCLVYLDESS
     LSSDILDLNA DDAIDFENVS NSAFLINNNI LYTYFSNTIL MKSKTDIHEI SSRGLSAHIL
     IYQILTKGDI IKPLTDIVNS LIGVEKNPIY DIIPRDKKSG KHGIIDIEKD IITH
//