Reviewed, UniProtKB/Swiss-Prot Q91HJ9 (VP3_ROTHU)

Names and origin

Protein names	Recommended name:     Protein VP3 Including the following 2 domains:     1- Recommended name:             mRNA guanylyltransferase               EC=2.7.7.50     2- Recommended name:             mRNA (guanine-N(7)-)-methyltransferase               EC=2.1.1.56
Organism	Rotavirus A (isolate Human/India/116E/1986 G9-P8[11]-I1-Rx-Cx-Mx-A1-Nx-Tx-E1-Hx) (RV-A)
Taxonomic identifier	42567 [NCBI]
Taxonomic lineage	Viruses › dsRNA viruses › Reoviridae › Rotavirus › Rotavirus A
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	291 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with VP1 Potential. Interacts with VP2 By similarity.
Subcellular location	Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.
Sequence similarities	Belongs to the rotavirus VP3 family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Core protein Virion
Ligand	GTP-binding Nucleotide-binding RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	viral nucleocapsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›291	›291	Protein VP3		PRO_0000368068
Regions
	Compositional bias	266 – 269	4	Poly-Tyr
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	291	1

Sequences

	Sequence		Length	Mass (Da)
	Q91HJ9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5AB0648715ED451F	FASTA	291	34,389
	10 20 30 40 50 60 ALRHSVAQVY ADTQTYDTDD SKDEYENAFL ISNLTTHNIL YLNYSVKTLK ILNKSGIAAI 70 80 90 100 110 120 EIQSPDELFT LIRCNFTYNY ENNTVYLHDY SYYTNNEIRT DQHWVTKTDI TNYLLPGWKL 130 140 150 160 170 180 TYVGYNGKNT RGHYKFSFIC QNAATVDDII IEYIYSNELD FQTFLLRKIK ERMTTSLPIT 190 200 210 220 230 240 RLSNRVFRDK LFSSILNTHK RVINVGPRNE SMFTFINLPT IKQFSNGAYL VKHSIKLKQE 250 260 270 280 290 RWLGKRVSQF DIGQYKNMLN VITTIYYYYN LHFSFPIIYM LGSAPSYWIY N

References

[1]	"Genetic characterization of VP3 gene of group A rotaviruses." Subodh S., Bhan M.K., Ray P. Virus Genes 33:143-145(2006) [PubMed: 16972027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY028978 Genomic RNA. Translation: AAK57549.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR009697. Rotavirus_VP3. [Graphical view]
Pfam	PF06929. Rotavirus_VP3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VP3_ROTHU

Accession

Primary (citable) accession number: Q91HJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)