Q91FE8 (RIR2_IIV6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ribonucleoside-diphosphate reductase small subunit 376L
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

ORF Names:

IIV6-376L

Organism

Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus) [Reference proteome]

Taxonomic identifier

176652 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Iridovirus

Virus host

Acheta domesticus (House cricket) [TaxID: 6997]
Chilo suppressalis (striped riceborer) [TaxID: 168631]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Gryllus campestris [TaxID: 58607]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Probable ribonucleoside-diphosphate reductase small subunit 376L		PRO_0000376910
Sites
	Active site	105	1	By similarity
	Metal binding	67	1	Iron 1 By similarity
	Metal binding	98	1	Iron 1 By similarity
	Metal binding	98	1	Iron 2 By similarity
	Metal binding	101	1	Iron 1 By similarity
	Metal binding	172	1	Iron 2 By similarity
	Metal binding	206	1	Iron 2 By similarity
	Metal binding	209	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91FE8 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D21CAE2D31961145	FASTA	360	42,154
	10 20 30 40 50 60 MSLLKGRNYY KPFTYPEFYN KWDKHEKSHW LPSEVPMHDD VNDWKNRLND NQRDFLTNIF 70 80 90 100 110 120 RFFTQGDVDV ASAYYTQYLP FFKLPEVTMM MGGFAGREGV HIDAYSYLLE TLGMPEATYK 130 140 150 160 170 180 EFLMYEEMKD KQDYIKKFSD SRHILGKGEE NLTTEDKEHI AAGIALFSGF TEGMQLFSTF 190 200 210 220 230 240 AMLLIFPLNG FMKGMGQIVT WSIVDETQHT EGMIELFKVF VEENKTGDQP IRPSVLQETV 250 260 270 280 290 300 YKIAKEMVGL EEAFIDLVFK KYKDEPINDD NDLETKDNKD FFGLTPQRLK AYIKYIADRR 310 320 330 340 350 360 LNLMGYKSIF NLEPYPTNPL PELEIMINAP THTNFFENRS TDYANVSTKG TWSEIWNKPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus." Jakob N.J., Mueller K., Bahr U., Darai G. Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes." Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R. Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF303741 Genomic DNA. Translation: AAK82236.1.
RefSeq	NP_149839.1. NC_003038.1.
3D structure databases
ProteinModelPortal	Q91FE8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1733046.
Phylogenomic databases
ProtClustDB	CLSP2511500.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_IIV6

Accession

Primary (citable) accession number: Q91FE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program