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Protein

Replication-associated protein

Gene

rep

Organism
Goose circovirus (GoCV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+Curated, Mn2+CuratedNote: Divalent metal cations, possibly Mg2+ or Mn2+.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Divalent metal cationSequence analysis1
Metal bindingi53Divalent metal cationSequence analysis1
Active sitei92For DNA cleavage activityBy similarity1
Metal bindingi96Divalent metal cationSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 175ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
Alternative name(s):
ATP-dependent helicase Rep
RepP
Gene namesi
Name:rep
OrganismiGoose circovirus (GoCV)
Taxonomic identifieri146032 [NCBI]
Taxonomic lineageiVirusesssDNA virusesCircoviridaeCircovirus
Virus hostiAnser (geese) [TaxID: 8842]
Proteomesi
  • UP000008626 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003198641 – 293Replication-associated proteinAdd BLAST293

Keywords - PTMi

Covalent protein-DNA linkage

Interactioni

Subunit structurei

Interacts with the capsid protein; this interaction relocates Rep into the nucleus.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ91EK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 13Nuclear localization signalSequence analysisAdd BLAST11
Motifi14 – 17RCR-1By similarity4
Motifi53 – 58RCR-2By similarity6
Motifi62 – 83Nuclear localization signalSequence analysisAdd BLAST22
Motifi92 – 95RCR-3By similarity4

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamiPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91EK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNGNYSYK RWVFTINNPT FEDYCAVVEF CNLDNCKFAI VGEEKGEKEG
60 70 80 90 100
TPHLQGFLSL RKNAKAAALE ENLGGRAWLS RARGSDEDNE EYCSKESTYL
110 120 130 140 150
RVGEPNRKGR SSDLPDAASD VLAGLPITDV ARKYPTTYVM FGRGLERLRQ
160 170 180 190 200
LIVETARDWK TEVIVLIGRP GSGKSRYAFE FPAREKYYKS RGKWWDGYNG
210 220 230 240 250
QDVVVMDDFY GWLPYDDLLR ICDRYPLRVE YKGGMTQFVA KTLIITSNRE
260 270 280 290
PRDWYKCEFD VSALYRRINQ YLILTPDGYA PAPEFMLPFK INY
Length:293
Mass (Da):33,842
Last modified:December 1, 2001 - v1
Checksum:iC626E2533B0D46D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ304456 Genomic DNA. Translation: CAC50261.1.
RefSeqiNP_150368.1. NC_003054.1.

Genome annotation databases

GeneIDi921700.
KEGGivg:921700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ304456 Genomic DNA. Translation: CAC50261.1.
RefSeqiNP_150368.1. NC_003054.1.

3D structure databases

ProteinModelPortaliQ91EK3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi921700.
KEGGivg:921700.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamiPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiREP_GOCV
AccessioniPrimary (citable) accession number: Q91EK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.