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Q91EK3

- REP_GOCV

UniProt

Q91EK3 - REP_GOCV

Protein

Replication-associated protein

Gene

rep

Organism
Goose circovirus (GoCV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Divalent metal cations, possibly magnesium or manganese.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Divalent metal cationSequence Analysis
    Metal bindingi53 – 531Divalent metal cationSequence Analysis
    Active sitei92 – 921For DNA cleavage activityBy similarity
    Metal bindingi96 – 961Divalent metal cationSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi168 – 1758ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity, uncoupled Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. DNA binding Source: UniProtKB-KW
    4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. metal ion binding Source: UniProtKB-KW
    6. nucleotidyltransferase activity Source: UniProtKB-KW
    7. RNA binding Source: InterPro
    8. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein-DNA covalent cross-linking Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
    Alternative name(s):
    ATP-dependent helicase Rep
    RepP
    Gene namesi
    Name:rep
    OrganismiGoose circovirus (GoCV)
    Taxonomic identifieri146032 [NCBI]
    Taxonomic lineageiVirusesssDNA virusesCircoviridaeCircovirus
    Virus hostiAnser (geese) [TaxID: 8842]
    ProteomesiUP000008626: Genome

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 293293Replication-associated proteinPRO_0000319864Add
    BLAST

    Keywords - PTMi

    Covalent protein-DNA linkage

    Interactioni

    Subunit structurei

    Interacts with the capsid protein; this interaction relocates Rep into the nucleus.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ91EK3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi3 – 1311Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi14 – 174RCR-1By similarity
    Motifi53 – 586RCR-2By similarity
    Motifi62 – 8322Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi92 – 954RCR-3By similarity

    Domaini

    There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR027417. P-loop_NTPase.
    IPR003365. Viral_rep_N.
    [Graphical view]
    PfamiPF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q91EK3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKNGNYSYK RWVFTINNPT FEDYCAVVEF CNLDNCKFAI VGEEKGEKEG    50
    TPHLQGFLSL RKNAKAAALE ENLGGRAWLS RARGSDEDNE EYCSKESTYL 100
    RVGEPNRKGR SSDLPDAASD VLAGLPITDV ARKYPTTYVM FGRGLERLRQ 150
    LIVETARDWK TEVIVLIGRP GSGKSRYAFE FPAREKYYKS RGKWWDGYNG 200
    QDVVVMDDFY GWLPYDDLLR ICDRYPLRVE YKGGMTQFVA KTLIITSNRE 250
    PRDWYKCEFD VSALYRRINQ YLILTPDGYA PAPEFMLPFK INY 293
    Length:293
    Mass (Da):33,842
    Last modified:December 1, 2001 - v1
    Checksum:iC626E2533B0D46D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ304456 Genomic DNA. Translation: CAC50261.1.
    RefSeqiNP_150368.1. NC_003054.1.

    Genome annotation databases

    GeneIDi921700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ304456 Genomic DNA. Translation: CAC50261.1 .
    RefSeqi NP_150368.1. NC_003054.1.

    3D structure databases

    ProteinModelPortali Q91EK3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 921700.

    Family and domain databases

    InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR027417. P-loop_NTPase.
    IPR003365. Viral_rep_N.
    [Graphical view ]
    Pfami PF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence determinations and analyses of novel circoviruses from goose and pigeon."
      Todd D., Weston J.H., Soike D., Smyth J.A.
      Virology 286:354-362(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiREP_GOCV
    AccessioniPrimary (citable) accession number: Q91EK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3