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Q91EK3 (REP_GOCV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication-associated protein

EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3
Alternative name(s):
ATP-dependent helicase Rep
RepP
Gene names
Name:rep
OrganismGoose circovirus (GoCV) [Complete proteome]
Taxonomic identifier146032 [NCBI]
Taxonomic lineageVirusesssDNA virusesCircoviridaeCircovirus
Virus hostAnser (geese) [TaxID: 8842]

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese Probable.

Subunit structure

Interacts with the capsid protein; this interaction relocates Rep into the nucleus By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the nanoviruses/circoviruses replication-associated protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 293293Replication-associated protein
PRO_0000319864

Regions

Nucleotide binding168 – 1758ATP By similarity
Motif3 – 1311Nuclear localization signal Potential
Motif14 – 174RCR-1 By similarity
Motif53 – 586RCR-2 By similarity
Motif62 – 8322Nuclear localization signal Potential
Motif92 – 954RCR-3 By similarity

Sites

Active site921For DNA cleavage activity By similarity
Metal binding441Divalent metal cation Potential
Metal binding531Divalent metal cation Potential
Metal binding961Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q91EK3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: C626E2533B0D46D2

FASTA29333,842
        10         20         30         40         50         60 
MAKNGNYSYK RWVFTINNPT FEDYCAVVEF CNLDNCKFAI VGEEKGEKEG TPHLQGFLSL 

        70         80         90        100        110        120 
RKNAKAAALE ENLGGRAWLS RARGSDEDNE EYCSKESTYL RVGEPNRKGR SSDLPDAASD 

       130        140        150        160        170        180 
VLAGLPITDV ARKYPTTYVM FGRGLERLRQ LIVETARDWK TEVIVLIGRP GSGKSRYAFE 

       190        200        210        220        230        240 
FPAREKYYKS RGKWWDGYNG QDVVVMDDFY GWLPYDDLLR ICDRYPLRVE YKGGMTQFVA 

       250        260        270        280        290 
KTLIITSNRE PRDWYKCEFD VSALYRRINQ YLILTPDGYA PAPEFMLPFK INY 

« Hide

References

[1]"Genome sequence determinations and analyses of novel circoviruses from goose and pigeon."
Todd D., Weston J.H., Soike D., Smyth J.A.
Virology 286:354-362(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ304456 Genomic DNA. Translation: CAC50261.1.
RefSeqNP_150368.1. NC_003054.1.

3D structure databases

ProteinModelPortalQ91EK3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID921700.

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameREP_GOCV
AccessionPrimary (citable) accession number: Q91EK3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families