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Q91E96 (VP2_ROTHC) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inner capsid protein VP2
OrganismRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C) [Reference proteome]
Taxonomic identifier31567 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length884 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Domain

The N-terminus is involved in VP1 binding By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   LigandRNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 884884Inner capsid protein VP2
PRO_0000369870

Regions

Region1 – 9696Interaction with VP1 and VP3 By similarity
Region427 – 44721Hydrophobic
Region506 – 52621Hydrophobic
Compositional bias376 – 3794Poly-Ala
Compositional bias822 – 8276Poly-Thr

Sequences

Sequence LengthMass (Da)Tools
Q91E96 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 3C0C7A4570C69E81

FASTA884101,673
        10         20         30         40         50         60 
MISRNRRRNN QQKNIEKEKQ LETIINKEVK ENKDSMKEDK LVVTEESNGD VTTAKEQSNN 

        70         80         90        100        110        120 
INLQKNDLVK EVMNIQNQTL NTVVTENKVE IEEIVKKYIP SYNTDSLIVK KLTEIQESSA 

       130        140        150        160        170        180 
KTYNTLFRLF TPVKSYLYDI NGEKKLSTRW YWKLLKDDLP AGDYSVRQFF LSLYLNVLEE 

       190        200        210        220        230        240 
MPDYIMLRDM AVDNPYSAEA GKIVDGKSKE ILIELYQDQM TEGYIRRYMS ELRHKISGET 

       250        260        270        280        290        300 
NTAKYPAILH PVDNELNQYF LEHQLIQPLT TRNIAELIPT QLYHDPNYVF NIDAAFLTNS 

       310        320        330        340        350        360 
RFVPPYLTQD RIGLHDGFES IWDSKTHADY VSARRFIPDL TELVDAEKQI KEMAAHLQLE 

       370        380        390        400        410        420 
AITVQVESQF LAGISAAAAN EAFKFIIGSV LSTRTIAVEF ITSNYMSLAS CMYLMTIMPS 

       430        440        450        460        470        480 
EIFLRESLVA MQLAIINTLI YPALGLAQMH YQAGEVRTPF ELAEMQVANR SIRQWLHHCN 

       490        500        510        520        530        540 
TLQFGRQITE GIIHLRFTND IMTGRIVNLF STMLVALSSQ PFATYPLDYK RSVQRALQLL 

       550        560        570        580        590        600 
SNRTAQIADL TRLIVYNYTT LSACIVMNMH LVGTLTVERI QATSLTSLMM LISNKTVIPE 

       610        620        630        640        650        660 
PSSLFSYFSS NINFLTNYNE QIDNVVAEIM AAYRLNLYQQ KMLMLVTRFV SKLYIFDAPK 

       670        680        690        700        710        720 
IPPDQMYRLR NRLRNIPVER RRADVFRIIM NNRDLIEKTS ERICQGVLLS YTPMPLTYVE 

       730        740        750        760        770        780 
DVGLTNVIND TNSFQIINIE EIEKTGDYSA ITNALLRDTP IILKGAIPYV TNSSVIDVLS 

       790        800        810        820        830        840 
KVDTTVFASI VKDRDISKLK PIKFIINSDS SEYYLVHNNK WTPTTTTAVY KARSQQFDIQ 

       850        860        870        880 
HSVSMLESNL FFVVYNDLFK YIKTTTVLPI NAVSYDGARI MQET 

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References

[1]"Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus."
Chen Z., Lambden P.R., Lau J., Caul E.O., Clarke I.N.
Virus Res. 83:179-187(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ303139 Genomic RNA. Translation: CAC44890.1.
RefSeqYP_392489.1. NC_007546.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3773136.

Family and domain databases

InterProIPR007779. Rotavirus_VP2.
[Graphical view]
PfamPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP2_ROTHC
AccessionPrimary (citable) accession number: Q91E96
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: October 16, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families