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Q91E95

- RDRP_ROTHC

UniProt

Q91E95 - RDRP_ROTHC

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Protein
RNA-directed RNA polymerase
Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium Reviewed prediction.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Magnesium, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifieri31567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007664: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

GO - Cellular componenti

  1. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10901090RNA-directed RNA polymerase
PRO_0000369869Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 Reviewed prediction. Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ91E95.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini506 – 678173RdRp catalytic
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91E95-1 [UniParc]FASTAAdd to Basket

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MAQSIVVDGD YDALASRYLK FVYDFENVTY QNNYFATDKF KKDIEQYLKS     50
IHDGEKITQS KIDEKEKILL DRVPAEERCL ISKLVFAYGK HGNVENKLVK 100
YGVKDALSHA PQKDAKPYEN NIITSEIFKE KSEYTDIYMD PSINTSCQSN 150
CQAMMFTISE MKLNNIKNAA RLEKLFTIIA ATINKYGMPR HNTRYRYEWE 200
TMKNKPYHLA AWINSSIEMI ACVVDHHTYM IARELIVKSF TNRTSLAKLV 250
SSPMTVLTAM LPIRGTFITT ENLELEYSNK SINYLISKEM AEDFMQAIKQ 300
LRDEGLEYIP DYYEKWFKSP DPLTFPNIAL IYSFSFHVGY RKQALSDAVY 350
DQITVTYSDN VNMEMYKEYS ERIENEIFTI LKDKIIHEDK RLEEYELSAL 400
LSMSSASNGI LREINFGGQK VRSTKKNMHV IDDIYHKKYT TDIPPVDARN 450
PIPLGRRDVP GRRTRAIFIL PYQYFIAQHS FAEIMLNYAK REREYSEFYS 500
QANQVLSYGD VTRYLDSNSI LCFTDVSQWD ASQHNTKVLR RSIIRAMKRL 550
KQLTHNINIH KAINIYIQSQ ENLENSYVLI DKKAIQYGAT ASGEKQTKIM 600
NSIANKALIQ TVLGKLMTDY TFDVKMIRVD GDDNYAIVRF PIAITEKLLS 650
EFTSKLRSYY SEMNVKVKAL ASLTGCEIAK RYVAGGMLFF RAGVNILHHE 700
KRNQDSAYDM AATLYANYIV NALRGLTMSR TFILTKICQM TSIKITGTLR 750
LFPMKSILAL NSAFKVFDEV DYVINYPISN LFIQLQRKLS SIKAKSKIAD 800
NIAKSPQFKS YVELLNKSLT TDENPIVSDG IRLTEKAKLN SYAPIALEKR 850
RDQFSIMVSF LQNPTTFKSE TVVTINDVLY FISGFIKIDS STVLPKEENN 900
TMPLLPAIIK RTLSYFGLRT HDYDIKGSSS TVSKIIKQYS VYTPGIEELY 950
EIVNKSVDTI RGYFASFNVP KADVDTYIST QMYKHDRFKI LQAYIYNLLS 1000
VNYGMYQLVD LNSARFFDHV IHTPMAKTPT AVFMIDLALR LKIINHCIEK 1050
GEIITVSVHA NKTDYLKLWR MLWNVKTMNS PYSKNSMFDE 1090
Length:1,090
Mass (Da):125,802
Last modified:December 1, 2001 - v1
Checksum:iA8B60957412342F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ304859 Genomic RNA. Translation: CAC44891.1.
RefSeqiYP_392464.1. NC_007547.1.

Genome annotation databases

GeneIDi3773137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ304859 Genomic RNA. Translation: CAC44891.1 .
RefSeqi YP_392464.1. NC_007547.1.

3D structure databases

ProteinModelPortali Q91E95.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3773137.

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus."
    Chen Z., Lambden P.R., Lau J., Caul E.O., Clarke I.N.
    Virus Res. 83:179-187(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_ROTHC
AccessioniPrimary (citable) accession number: Q91E95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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