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Q91E95

- RDRP_ROTHC

UniProt

Q91E95 - RDRP_ROTHC

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription (By similarity).PROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Cofactori

Mg2+Curated

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Magnesium, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifieri31567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007664: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (By similarity).By similarity

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10901090RNA-directed RNA polymerasePRO_0000369869Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 (Potential). Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak (By similarity).By similarityCurated

Structurei

3D structure databases

ProteinModelPortaliQ91E95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini506 – 678173RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91E95-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQSIVVDGD YDALASRYLK FVYDFENVTY QNNYFATDKF KKDIEQYLKS
60 70 80 90 100
IHDGEKITQS KIDEKEKILL DRVPAEERCL ISKLVFAYGK HGNVENKLVK
110 120 130 140 150
YGVKDALSHA PQKDAKPYEN NIITSEIFKE KSEYTDIYMD PSINTSCQSN
160 170 180 190 200
CQAMMFTISE MKLNNIKNAA RLEKLFTIIA ATINKYGMPR HNTRYRYEWE
210 220 230 240 250
TMKNKPYHLA AWINSSIEMI ACVVDHHTYM IARELIVKSF TNRTSLAKLV
260 270 280 290 300
SSPMTVLTAM LPIRGTFITT ENLELEYSNK SINYLISKEM AEDFMQAIKQ
310 320 330 340 350
LRDEGLEYIP DYYEKWFKSP DPLTFPNIAL IYSFSFHVGY RKQALSDAVY
360 370 380 390 400
DQITVTYSDN VNMEMYKEYS ERIENEIFTI LKDKIIHEDK RLEEYELSAL
410 420 430 440 450
LSMSSASNGI LREINFGGQK VRSTKKNMHV IDDIYHKKYT TDIPPVDARN
460 470 480 490 500
PIPLGRRDVP GRRTRAIFIL PYQYFIAQHS FAEIMLNYAK REREYSEFYS
510 520 530 540 550
QANQVLSYGD VTRYLDSNSI LCFTDVSQWD ASQHNTKVLR RSIIRAMKRL
560 570 580 590 600
KQLTHNINIH KAINIYIQSQ ENLENSYVLI DKKAIQYGAT ASGEKQTKIM
610 620 630 640 650
NSIANKALIQ TVLGKLMTDY TFDVKMIRVD GDDNYAIVRF PIAITEKLLS
660 670 680 690 700
EFTSKLRSYY SEMNVKVKAL ASLTGCEIAK RYVAGGMLFF RAGVNILHHE
710 720 730 740 750
KRNQDSAYDM AATLYANYIV NALRGLTMSR TFILTKICQM TSIKITGTLR
760 770 780 790 800
LFPMKSILAL NSAFKVFDEV DYVINYPISN LFIQLQRKLS SIKAKSKIAD
810 820 830 840 850
NIAKSPQFKS YVELLNKSLT TDENPIVSDG IRLTEKAKLN SYAPIALEKR
860 870 880 890 900
RDQFSIMVSF LQNPTTFKSE TVVTINDVLY FISGFIKIDS STVLPKEENN
910 920 930 940 950
TMPLLPAIIK RTLSYFGLRT HDYDIKGSSS TVSKIIKQYS VYTPGIEELY
960 970 980 990 1000
EIVNKSVDTI RGYFASFNVP KADVDTYIST QMYKHDRFKI LQAYIYNLLS
1010 1020 1030 1040 1050
VNYGMYQLVD LNSARFFDHV IHTPMAKTPT AVFMIDLALR LKIINHCIEK
1060 1070 1080 1090
GEIITVSVHA NKTDYLKLWR MLWNVKTMNS PYSKNSMFDE
Length:1,090
Mass (Da):125,802
Last modified:December 1, 2001 - v1
Checksum:iA8B60957412342F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ304859 Genomic RNA. Translation: CAC44891.1.
RefSeqiYP_392464.1. NC_007547.1.

Genome annotation databases

GeneIDi3773137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ304859 Genomic RNA. Translation: CAC44891.1 .
RefSeqi YP_392464.1. NC_007547.1.

3D structure databases

ProteinModelPortali Q91E95.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3773137.

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus."
    Chen Z., Lambden P.R., Lau J., Caul E.O., Clarke I.N.
    Virus Res. 83:179-187(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_ROTHC
AccessioniPrimary (citable) accession number: Q91E95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3