Reviewed, UniProtKB/Swiss-Prot Q91DR9 (L_SVCV)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Spring viremia of carp virus (Rhabdovirus carpia)

Taxonomic identifier

11275 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Vesiculovirus

Virus host

Cyprinus carpio (Common carp) [TaxID: 7962]

Protein attributes

Sequence length	2095 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subunit structure	Interacts with the P protein to form the functional polymerase By similarity.
Subcellular location	Virion. CytoplasmBy similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2095	2095	Large structural protein		PRO_0000287261
Regions
	Domain	587 – 773	187	RdRp catalytic
	Nucleotide binding	1652 – 1661	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q91DR9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: EB43D47D061379AC	FASTA	2,095	238,192
	10 20 30 40 50 60 MFEWESQDTP SGLPDEESYF PTSKLSVEER MHYLNNVDYN LNSPLISDDI EYLTLKHFGR 70 80 90 100 110 120 AIPSLWKVKN WEIPLEMLKG VGIIKTWDQI HPWMGKWFDS EHNCPQGESF LRTVQAESEL 130 140 150 160 170 180 TSEIPVTFIK GWIGKEIKFP VKRGHHAVHL LMQKVLDLHK LTLLINSVDS GETEKLCESF 190 200 210 220 230 240 GLNSKQSKFE TYSLGTVRYC PGWIFIDKAE ILLDRNFLLM MKDTLIGRLQ TLLSMLGNCE 250 260 270 280 290 300 MEVEQIYTHT ETMLSLYSYG DQIIEKAGNN GYSKIKLLEP ICNLRLSELA HKYRPLVPDF 310 320 330 340 350 360 PHFQEHVETS VREEDTTDGL LSAILSLVNN TEDIQLILTI YGSFRHWGHP FISYFEGLQK 370 380 390 400 410 420 LHDQVTLPKQ IDKEYAAALA SDLAYTVLQR KFSEEKKWYV DSIALSSKHP LKEHVDNGTW 430 440 450 460 470 480 PTAAQIQDFG DRWHLLPLTK CFEVPDLLDP SVIYSDKSHS MNRKEVIDHV ISTPNKPIPS 490 500 510 520 530 540 KKVLETMINN PATDWPTFLK AVDEEGLPRD NLIIGLKGKE RELKIAGRFF SLMSWQLREY 550 560 570 580 590 600 FVITEYLIKT HYVPLFKGLT MADDLTSVVK KMLDNTNGQG LDDYSSICIA NHIDYEKWNN 610 620 630 640 650 660 HQRKESNGPV FRVMGQFLGY PRLFERTHEF FESSLIYYNG RPDLMDVRGD SLVNTTDKMV 670 680 690 700 710 720 CWEGQAGGLE GLRQKGWSVL NLLVINRESS IRNTVVKVLA QGDNQVICTQ YKTKNYKNED 730 740 750 760 770 780 ELKMLLTAMV ENNQTIMNGI ITGTGKLGLI INNDETMQSA DYLNYGKVPV FRGILRGLET 790 800 810 820 830 840 KRWSRVTCIT NDQIPTLAGV MSSVSTNALT VAHFAASPIN AILQYHYFAN FCLMMIAMHN 850 860 870 880 890 900 PAIRSSMYTK MFRKCHIMSK EFKAATLYLD PSLGGVCGIS LARFLIRSFP DPVTEGLAFW 910 920 930 940 950 960 KMIHHNCQSD WLKALSKRCG NPKLARFRPE HIPKIIEDPA ALNISMGMSA SNLLKTEVKG 970 980 990 1000 1010 1020 HLIRTADTIQ NQIIREAAEY LGQEEASLNE FLWDIEPFFP RFLSEFRSST FVGVTDSLIG 1030 1040 1050 1060 1070 1080 LFQNSKTIRG LFKSYYKREL DRLVVKSELS SLEHLGSYRK ETPDSIWECS STQADLLREK 1090 1100 1110 1120 1130 1140 SWGRSVIGMT VPHPLEMFGK GHQKELECIP CQTSGLTYIS SYCPRGINNW YSTVGSLAAY 1150 1160 1170 1180 1190 1200 LGSKTSETTS ILQPWEKDSK IPIIKRATKL RDSISWFVPP DSKLAKSIQQ NLKALTGEDW 1210 1220 1230 1240 1250 1260 EEDIQGFKRT GSALHRFTTS RVSNGGFSAQ SPAKLTRIMT TTDTMRDLGD QNYDFMFQAG 1270 1280 1290 1300 1310 1320 ILYSQMTTGE LRENSTNSTA THYHITCKSC LREIQEPMLE SRIVYNPPSS SRVIKSWIPN 1330 1340 1350 1360 1370 1380 ATEIMEESKP IKLREVDWDP LTRYEKSYHI GRCQGFLYGD LTYQKTGRSE ESSIFPLSIQ 1390 1400 1410 1420 1430 1440 YKVEGSGFMR GFCDGIIRAS AVQALHRRVS SIVSTADVIY GGALYLTNQV GDSPPFQNLC 1450 1460 1470 1480 1490 1500 RSGPLREELE RIPHKMTSSY PTSNSDMGYL IRNYLKRSLK QLSRGRYETK EGPIWVFSDV 1510 1520 1530 1540 1550 1560 RTKKFLGPFS LSTDALNCLY KNKLSKRDKN AVRNLSQLSS RMRSGDLSDE EIGKVEARFS 1570 1580 1590 1600 1610 1620 FTPAEMRHAC KFTIGKTQVP IVMSEWGQEA YGNITMYPVF YSTTKTEKPD WTFSRLQNPT 1630 1640 1650 1660 1670 1680 ISGLRISQQA TGAHYKLRSL LKGMKIHYQD AIGCGDGSGG LSSCLLRENK HCRVIFNSLL 1690 1700 1710 1720 1730 1740 ELTGNTLRGS TPDPPSAING IPQIRDRCVN LNNVWEHPSD LSHPDTWKYF GELKAQFNMD 1750 1760 1770 1780 1790 1800 VDLIVMDMEV QDIDISRRIE QNLRDHVHSL LSRHGTVIYK TYMTIMSENE KSVLDIVGVL 1810 1820 1830 1840 1850 1860 FEDVQLCQTQ YSSSQTSEVY CVMRRLRQKV DSQHVDWQSL VRQGINSKVY CNLPLDKEFE 1870 1880 1890 1900 1910 1920 RALNLYQIDT LVGVPRGLIP NLAVELETLL EIGGLSGGIL GKLVLNIEEG KLGFTMALIV 1930 1940 1950 1960 1970 1980 SCILISESAI CTTRLSNKRE VPSSGACQRM AVCLIGAAIL LSVHHRSIEN HKGAIRMLRH 1990 2000 2010 2020 2030 2040 SVPIRISSKL RKDGKLQSRW SSISREGLAK DVRLNSNMAG VGAWIRVWSR MKDRERRWEA 2050 2060 2070 2080 2090 READSWLKTH NKGLSMEHVR RHTGVLDILH GTGDRLDRSV PTVSSAPRES GTWVE

References

[1]

"Determination of the complete genomic sequence and analysis of the gene products of the virus of Spring Viremia of Carp, a fish rhabdovirus." Hoffmann B., Schutze H., Mettenleiter T.C. Virus Res. 84:89-100(2002) [PubMed: 11900842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Fijan reference.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ318079 Genomic RNA. Translation: CAC51337.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017234. RNA-dir_RNA_pol_rhabdovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_SVCV

Accession

Primary (citable) accession number: Q91DR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)