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Q91DE0

- VP35_EBORE

UniProt

Q91DE0 - VP35_EBORE

Protein

Polymerase cofactor VP35

Gene

VP35

Organism
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition host IKBKE and TBK1 producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host IKBKE activity Source: UniProtKB-KW
    2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
    3. suppression by virus of host TBK1 activity Source: UniProtKB-KW
    4. suppression by virus of host toll-like receptor signaling pathway Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host IKBKE by virus, Inhibition of host innate immune response by virus, Inhibition of host IRF7 by virus, Inhibition of host RLR pathway by virus, Inhibition of host TBK1 by virus, Inhibition of host TLR pathway by virus, Interferon antiviral system evasion, Transcription, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase cofactor VP35
    Gene namesi
    Name:VP35
    OrganismiReston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
    Taxonomic identifieri129003 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) [TaxID: 9541]
    Pteropodinae [TaxID: 77225]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000002322: Genome

    Subcellular locationi

    Virion. Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Polymerase cofactor VP35PRO_0000245075Add
    BLAST

    Post-translational modificationi

    Phosphorylated by host IKBKE.By similarity

    Interactioni

    Subunit structurei

    Homooligomer. Homomultimerizes via the coiled coil domain. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein By similarity. May interact with VP40. Interacts with host IKBKE and TBK1; the interactions lead to inhibition of cellular antiviral response by blocking necessary interactions of IKBKE and TBK1 with their substrate IRF3 By similarity.By similarity

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili84 – 11229Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002953. Filo_VP35.
    [Graphical view]
    PfamiPF02097. Filo_VP35. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
    PRINTSiPR01240. FILOVP35.

    Sequencei

    Sequence statusi: Complete.

    Q91DE0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNDKLKICS GPETTGWISE QLMTGKIPVT DIFIDIDNKP DQMEVRLKPS    50
    SRSSTRTCTS SSQTEVNYVP LLKKVEDTLT MLVSATSRQN AAIEALENRL 100
    STLESSLKPI QDMGKVISSL NRSCAEMVAK YDLLVMTTGR ATSTAAAVDA 150
    YWKEHKQPPP GPALYEENAL KGKIDDPNSY VPDAVQEAYK NLDSTSTLTE 200
    ENFGKPYISA KDLKEIMYDH LPGFGTAFHQ LVQVICKIGK DNNLLDTIHA 250
    EFQASLADGD SPQCALIQIT KRVPIFQDVP PPTIHIRSRG DIPRACQKSL 300
    RPAPPSPKID RGWVCLFKMQ DGKTLGLKI 329
    Length:329
    Mass (Da):36,386
    Last modified:December 1, 2001 - v1
    Checksum:i643EEC62F73D8861
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB050936 Genomic RNA. Translation: BAB69004.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB050936 Genomic RNA. Translation: BAB69004.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002953. Filo_VP35.
    [Graphical view ]
    Pfami PF02097. Filo_VP35. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018326. VP35_FiloV. 1 hit.
    PRINTSi PR01240. FILOVP35.
    ProtoNeti Search...

    Publicationsi

    1. "Genome structure of Ebola virus subtype Reston: differences among Ebola subtypes."
      Ikegami T., Calaor A.B., Miranda M.E., Niikura M., Saijo M., Kurane I., Yoshikawa Y., Morikawa S.
      Arch. Virol. 146:2021-2027(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiVP35_EBORE
    AccessioniPrimary (citable) accession number: Q91DE0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3