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Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene namesi
Name:VP30
OrganismiReston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
Taxonomic identifieri129003 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) [TaxID: 9541]
Pteropodinae [TaxID: 77225]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000002322: Genome

Subcellular locationi

Virion. Host cytoplasm By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Minor nucleoprotein VP30PRO_0000245071Add
BLAST

Post-translational modificationi

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer or homodimer. Interacts with the nucleoprotein (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ91DD6.
SMRiQ91DD6. Positions 142-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Intrinsically disorderedSequence AnalysisAdd
BLAST
Regioni26 – 4015RNA-bindingBy similarityAdd
BLAST
Regioni94 – 11219OligomerizationBy similarityAdd
BLAST
Regioni121 – 14020Intrinsically disorderedSequence AnalysisAdd
BLAST
Regioni180 – 19718Interaction with the nucleoproteinBy similarityAdd
BLAST
Regioni268 – 28720Intrinsically disorderedSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91DD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHSRERGRS SNMRHNSREP YENPSRSRSL SRDPNQVDRR QPRSASQIRV
60 70 80 90 100
PNLFHRKKTD ALIVPPAPKD ICPTLKKGFL CDSKFCKKDH QLDSLNDHEL
110 120 130 140 150
LLLIARRTCG IIESNSQITS PKDMRLANPT AEDFSQGNSP KLTLAVLLQI
160 170 180 190 200
AEHWATRDLR QIEDSKLRAL LTLCAVLTRK FSKSQLGLLC ETHLRHEGLG
210 220 230 240 250
QDQADSVLEV YQRLHSDKGG NFEAALWQQW DRQSLIMFIS AFLNIALQTP
260 270 280
CESSSVVVSG LATLYPAQDN STPSEATNDT TWSSTVE
Length:287
Mass (Da):32,389
Last modified:December 1, 2001 - v1
Checksum:iF4CFBDA604A77920
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050936 Genomic RNA. Translation: BAB69008.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050936 Genomic RNA. Translation: BAB69008.1.

3D structure databases

ProteinModelPortaliQ91DD6.
SMRiQ91DD6. Positions 142-266.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome structure of Ebola virus subtype Reston: differences among Ebola subtypes."
    Ikegami T., Calaor A.B., Miranda M.E., Niikura M., Saijo M., Kurane I., Yoshikawa Y., Morikawa S.
    Arch. Virol. 146:2021-2027(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP30_EBORE
AccessioniPrimary (citable) accession number: Q91DD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.