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Q91DD4

- L_EBORE

UniProt

Q91DD4 - L_EBORE

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Protein
RNA-directed RNA polymerase L
Gene
L
Organism
Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiReston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)
Taxonomic identifieri129003 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) [TaxID: 9541]
Pteropodinae [TaxID: 77225]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000002322: Genome

Subcellular locationi

Host cytoplasm. Virion By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22122212RNA-directed RNA polymerase L
PRO_0000245047Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini625 – 809185RdRp catalytic
Add
BLAST
Domaini1803 – 2001199Mononegavirus-type SAM-dependent 2'-O-MTase
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91DD4-1 [UniParc]FASTAAdd to Basket

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MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRQCKLPKHI     50
YRLKFDTIVS KFLSDTPVAT LPIDYLVPIL LRSLTGHGDR PLTPTCNQFL 100
DGIINYTLHD AAFLDYYLKA TGAQDHLTNI TTREKLKNEI LNNDYVHQLF 150
FWHDLSILAR RGRLNRGNNR STWFVHDEFI DILGYGDYIF WKIPLSLLPV 200
TIDGVPHAAT DWYQPTLFKE SILGHSQILS VSTAEILIMC KDIITCRFNT 250
SLIASIAKLE DVDVSDYPDP SDILKIYNAG DYVISILGSE GYKIIKYLEP 300
LCLAKIQLCS KFTERKGRFL TQMHLSVIND LRELISNRRL KDYQQEKIRD 350
FHKILLQLQL SPQQFCELFS VQKHWGHPIL HSEKAIQKVK RHATILKALR 400
PNVIFETYCV FKYNIAKHYF DSQGTWYSVI SDRNLTPGLN SFIKRNHFPS 450
LPMIKDLLWE FYHLNHPPLF STKVISDLSI FIKDRATAVE QTCWDAVFEP 500
NVLGYNPPNK FSTKRVPEQF LEQEDFSIES VLNYAQELHY LLPQNRNFSF 550
SLKEKELNIG RTFGKLPYLT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 600
QKESLLHQAS WHHTSDDFGE NATVRGSSFV TDLEKYNLAF RYEFTAPFIE 650
YCNHCYGVRN VFNWMHYLIP QCYMHVSDYY NPPHNVNLSN REYPPEGPSS 700
YRGHLGGIEG LQQKLWTSIS CAQISLVEIK TGFKLRSAVM GDNQCITVLS 750
VFPLETDPEE QEQSAEDNAA RVAASLAKVT SACGIFLKPE ETFVHSGFIY 800
FGKKQYLNGV QLPQSLKTAA RMAPLSDAIF DDLQGTLASI GTAFERAISE 850
TRHILPCRIV AAFHTYFAVR ILQYHHLGFN KGIDLGQLSL SKPLDYGTIT 900
LTLAVPQVLG GLSFLNPEKC FYRNFGDPVT SGLFQLRVYL EMVNMKDLFY 950
PLISKNPGNC SAIDFVLNPS GLNVPGSQDL TSFLRQIVRR SITLTARNKL 1000
INTLFHASAD LEDEMVCKWL LSSNPVMSRF AADIFSRTPS GKRLQILGYL 1050
EGTRTLLASK IINNNSETPV LDKLRKITLQ RWNLWFSYLD HCDQLLADAL 1100
QKISCTVDLA QILREYTWSH ILEGRPLIGA TLPCMVEQFK VKWLRQYEPC 1150
PECLNKKGSN AYVSVAVKDQ VVSAWPNTSR ISWTIGSGVP YIGSRTEDKI 1200
GQPAIKPRCP SSALKEAIEL ASRLTWVTQG SSNSEQLIRP FLEARVNLSV 1250
SEVLQMTPSH YSGNIVHRYN DQYSPHSFMA NRMSNTATRL IVSTNTLGEF 1300
SGGGQAARDS NIIFQNVINL AVALYDIRFR NTNTSDIRHN RAHLHLTECC 1350
TKEVPAQYLT YTSALNLDLS RYRDNELIYD SNPLRGGLNC NLTMDSPLVK 1400
GPRLNMIEDD LLRFPHLSGW ELAKTVVQSI ISDNSNSSTD PISSGETRSF 1450
TTHFLTYPQI GLLYSFGAVL CFYLGNTILW TKKLDYEQFL YYLHNQLHNL 1500
PHRALRVFKP TFKHASVMSR LMEIDSNFSI YIGGTSGDRG LSDAARLFLR 1550
TAIASFLQFL KSWIIDRQKA IPLWIVYPLE GQQPESINEF LHKIFGLLKQ 1600
GPKNIPKEVS IQNDGHLDLA ENNYVYNSKS TASNFFHASL AYWRSRKSRK 1650
TQDHNDFSRG DGTLTEPVCK FSSNHQSDEK YYNVTCGKSP KPQERKDFSQ 1700
YRLSNNGQTM SNHRKKGKFH KWNPCKVLME SQRGTVLKEG DYFQNNTPPT 1750
DDVSSPHRLI LPFFKLGNHN HAHDQDAQEL INQNIKQYLH QLRSMLDTTI 1800
YCRFTGIVSS MHYKLDEVLL EYNSFDSAIT LAEGEGSGAL LLLQKYSTRL 1850
LFLNTLATEH SIESEVVSGF STPRMLLPIM QKVHEGQVTV ILNNSASQIT 1900
DITSSMWLSN QKYNLPCQVE IITMDAETTE NLNRSQLYRA VYNLILDHID 1950
PQYLKVVVLK VFLSDIEGIL WINDYLAPLF GAGYLIKPIT SSARSSEWYL 2000
CLSNLISTNR RSAHQTHKAC LGVIRDALQA QVQRGVYWLS HIAQYATKNL 2050
HCEYICLGFP PLEKVLYHRY NLVDTGLGPL SSVIRHLTNL QAEIRDLVLD 2100
YTLMRESRTQ TYHFIKTAKG RITKLVNDFL KFSLIVQALK NNSSWYTELK 2150
KLPEVINVCN RFYHTHSCEC QEKFFVQTLY LQRLRDAEIK LIERLTGLMR 2200
FYPEGLIYSN HT 2212
Length:2,212
Mass (Da):252,708
Last modified:December 1, 2001 - v1
Checksum:i82100B1C4BF79DDC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB050936 Genomic RNA. Translation: BAB69010.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB050936 Genomic RNA. Translation: BAB69010.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome structure of Ebola virus subtype Reston: differences among Ebola subtypes."
    Ikegami T., Calaor A.B., Miranda M.E., Niikura M., Saijo M., Kurane I., Yoshikawa Y., Morikawa S.
    Arch. Virol. 146:2021-2027(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_EBORE
AccessioniPrimary (citable) accession number: Q91DD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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