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Q91AV1

- SPIKE_PEDV7

UniProt

Q91AV1 - SPIKE_PEDV7

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Protein

Spike glycoprotein

Gene

S

Organism
Porcine epidemic diarrhea virus (strain CV777) (PEDV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

S1 region attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity).By similarity

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
  2. pathogenesis Source: UniProtKB-KW
  3. receptor-mediated virion attachment to host cell Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virulence, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spike glycoprotein
Short name:
S glycoprotein
Alternative name(s):
E2
Peplomer protein
Gene namesi
Name:S
ORF Names:2
OrganismiPorcine epidemic diarrhea virus (strain CV777) (PEDV)
Taxonomic identifieri229032 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008159: Genome

Subcellular locationi

Virion membrane By similarity; Single-pass type I membrane protein By similarity. Host endoplasmic reticulum-Golgi intermediate compartment membrane By similarity; Single-pass type I membrane protein By similarity
Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Some S oligomers may be transported to the plasma membrane, where they may mediate cell-cell fusion (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 13241306Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei1325 – 134420HelicalSequence AnalysisAdd
BLAST
Topological domaini1345 – 138339IntravirionSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 13831365Spike glycoproteinPRO_0000283925Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi127 – 1271N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi213 – 2131N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi230 – 2301N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi261 – 2611N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi321 – 3211N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi341 – 3411N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi348 – 3481N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi378 – 3781N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi422 – 4221N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi511 – 5111N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi553 – 5531N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi664 – 6641N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi685 – 6851N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi719 – 7191N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi723 – 7231N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi740 – 7401N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi778 – 7781N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi784 – 7841N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi870 – 8701N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1006 – 10061N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1229 – 12291N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1246 – 12461N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1258 – 12581N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1270 – 12701N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1275 – 12751N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1292 – 12921N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1305 – 13051N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

IntActiQ91AV1. 3 interactions.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4J79X-ray1.56B1378-1383[»]
ProteinModelPortaliQ91AV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 734718S1By similarityAdd
BLAST
Regioni735 – 1383649S2By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1036 – 108045Sequence AnalysisAdd
BLAST
Coiled coili1272 – 131443Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1379 – 13835KxHxxBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1345 – 136622Cys-richAdd
BLAST

Domaini

The KxHxx motif seems to function as an ER retrieval signal.By similarity

Sequence similaritiesi

Belongs to the coronaviruses spike protein family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.5.790. 1 hit.
InterProiIPR002551. Corona_S1.
IPR002552. Corona_S2.
IPR027400. S_HR2.
[Graphical view]
PfamiPF01600. Corona_S1. 1 hit.
PF01601. Corona_S2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91AV1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRSLIYFWLL LPVLPTLSLP QDVTRCQSTT NFRRFFSKFN VQAPAVVVLG
60 70 80 90 100
GYLPSMNSSS WYCGTGIETA SGVHGIFLSY IDSGQGFEIG ISQEPFDPSG
110 120 130 140 150
YQLYLHKATN GNTNAIARLR ICQFPDNKTL GPTVNDVTTG RNCLFNKAIP
160 170 180 190 200
AYMRDGKDIV VGITWDNDRV TVFADKIYHF YLKNDWSRVA TRCYNRRSCA
210 220 230 240 250
MQYVYTPTYY MLNVTSAGED GIYYEPCTAN CTGYAANVFA TDSNGHIPEG
260 270 280 290 300
FSFNNWFLLS NDSTLLHGKV VSNQPLLVNC LLAIPKIYGL GQFFSFNHTM
310 320 330 340 350
DGVCNGAAVD RAPEALRFNI NDTSVILAEG SIVLHTALGT NLSFVCSNSS
360 370 380 390 400
DPHLAIFAIP LGATEVPYYC FLKVDTYNST VYKFLAVLPP TVREIVITKY
410 420 430 440 450
GDVYVNGFGY LHLGLLDAVT INFTGHGTDD DVSGFWTIAS TNFVDALIEV
460 470 480 490 500
QGTSIQRILY CDDPVSQLKC SQVAFDLDDG FYPISSRNLL SHEQPISFVT
510 520 530 540 550
LPSFNDHSFV NITVSAAFGG LSSANLVASD TTINGFSSFC VDTRQFTITL
560 570 580 590 600
FYNVTNSYGY VSKSQDSNCP FTLQSVNDYL SFSKFCVSTS LLAGACTIDL
610 620 630 640 650
FGYPAFGSGV KLTSLYFQFT KGELITGTPK PLEGITDVSF MTLDVCTKYT
660 670 680 690 700
IYGFKGEGII TLTNSSILAG VYYTSDSGQL LAFKNVTSGA VYSVTPCSFS
710 720 730 740 750
EQAAYVNDDI VGVISSLSNS TFNNTRELPG FFYHSNDGSN CTEPVLVYSN
760 770 780 790 800
IGVCKSGSIG YVPSQYGQVK IAPTVTGNIS IPTNFSMSIR TEYLQLYNTP
810 820 830 840 850
VSVDCATYVC NGNSRCKQLL TQYTAACKTI ESALQLSARL ESVEVNSMLT
860 870 880 890 900
ISEEALQLAT ISSFNGDGYN FTNVLGASVY DPASGRVVQK RSVIEDLLFN
910 920 930 940 950
KVVTNGLGTV DEDYKRCSNG RSVADLVCAQ YYSGVMVLPG VVDAEKLHMY
960 970 980 990 1000
SASLIGGMAL GGITAAAALP FSYAVQARLN YLALQTDVLQ RNQQLLAESF
1010 1020 1030 1040 1050
NSAIGNITSA FESVKEAISQ TSKGLNTVAH ALTKVQEVVN SQGSALNQLT
1060 1070 1080 1090 1100
VQLQHNFQAI SSSIDDIYSR LDILSADVQV DRLITGRLSA LNAFVAQTLT
1110 1120 1130 1140 1150
KYTEVQASRK LAQQKVNECV KSQSQRYGFC GGDGEHIFSL VQAAPQGLLF
1160 1170 1180 1190 1200
LHTVLVPGDF VNVLAIAGLC VNGEIALTLR EPGLVLFTHE LQTYTATEYF
1210 1220 1230 1240 1250
VSSRRMFEPR KPTVSDFVQI ESCVVTYVNL TSDQLPDVIP DYIDVNKTLD
1260 1270 1280 1290 1300
EILASLPNRT GPSLPLDVFN ATYLNLTGEI ADLEQRSESL RNTTEELRSL
1310 1320 1330 1340 1350
INNINNTLVD LEWLNRVETY IKWPWWVWLI IVIVLIFVVS LLVFCCISTG
1360 1370 1380
CCGCCGCCGA CFSGCCRGPR LQPYEAFEKV HVQ
Length:1,383
Mass (Da):151,353
Last modified:December 1, 2001 - v1
Checksum:i022E5E5E5435876D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353511 Genomic RNA. Translation: AAK38656.1.
RefSeqiNP_598310.1. NC_003436.1.

Genome annotation databases

GeneIDi935184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353511 Genomic RNA. Translation: AAK38656.1 .
RefSeqi NP_598310.1. NC_003436.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4J79 X-ray 1.56 B 1378-1383 [» ]
ProteinModelPortali Q91AV1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91AV1. 3 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 935184.

Family and domain databases

Gene3Di 1.20.5.790. 1 hit.
InterProi IPR002551. Corona_S1.
IPR002552. Corona_S2.
IPR027400. S_HR2.
[Graphical view ]
Pfami PF01600. Corona_S1. 1 hit.
PF01601. Corona_S2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF."
    Duarte M., Tobler K., Bridgen A., Rasschaert D., Ackermann M., Laude H.
    Virology 198:466-476(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Further analysis of the genome of porcine epidemic diarrhea virus."
    Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.
    Adv. Exp. Med. Biol. 440:781-786(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence."
    Kocherhans R., Bridgen A., Ackermann M., Tobler K.
    Virus Genes 23:137-144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiSPIKE_PEDV7
AccessioniPrimary (citable) accession number: Q91AV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

In contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2.Curated

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3