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Q91AV1

- SPIKE_PEDV7

UniProt

Q91AV1 - SPIKE_PEDV7

Protein

Spike glycoprotein

Gene

S

Organism
Porcine epidemic diarrhea virus (strain CV777) (PEDV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    S1 region attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes By similarity.By similarity

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro
    2. pathogenesis Source: UniProtKB-KW
    3. receptor-mediated virion attachment to host cell Source: InterPro

    Keywords - Biological processi

    Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virulence, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spike glycoprotein
    Short name:
    S glycoprotein
    Alternative name(s):
    E2
    Peplomer protein
    Gene namesi
    Name:S
    ORF Names:2
    OrganismiPorcine epidemic diarrhea virus (strain CV777) (PEDV)
    Taxonomic identifieri229032 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008159: Genome

    Subcellular locationi

    Virion membrane By similarity; Single-pass type I membrane protein By similarity. Host endoplasmic reticulum-Golgi intermediate compartment membrane By similarity; Single-pass type I membrane protein By similarity
    Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Some S oligomers may be transported to the plasma membrane, where they may mediate cell-cell fusion By similarity.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 13831365Spike glycoproteinPRO_0000283925Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi127 – 1271N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi213 – 2131N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi261 – 2611N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi321 – 3211N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi341 – 3411N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi348 – 3481N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi378 – 3781N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi422 – 4221N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi511 – 5111N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi553 – 5531N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi664 – 6641N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi685 – 6851N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi719 – 7191N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi723 – 7231N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi740 – 7401N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi778 – 7781N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi784 – 7841N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi870 – 8701N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1006 – 10061N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1229 – 12291N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1246 – 12461N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1258 – 12581N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1270 – 12701N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1275 – 12751N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1292 – 12921N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi1305 – 13051N-linked (GlcNAc...); by hostSequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer.By similarity

    Protein-protein interaction databases

    IntActiQ91AV1. 3 interactions.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4J79X-ray1.56B1378-1383[»]
    ProteinModelPortaliQ91AV1.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 13241306Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini1345 – 138339IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1325 – 134420HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni17 – 734718S1By similarityAdd
    BLAST
    Regioni735 – 1383649S2By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1036 – 108045Sequence AnalysisAdd
    BLAST
    Coiled coili1272 – 131443Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1379 – 13835KxHxxBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1345 – 136622Cys-richAdd
    BLAST

    Domaini

    The KxHxx motif seems to function as an ER retrieval signal.By similarity

    Sequence similaritiesi

    Belongs to the coronaviruses spike protein family.Curated

    Keywords - Domaini

    Coiled coil, Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.5.790. 1 hit.
    InterProiIPR002551. Corona_S1.
    IPR002552. Corona_S2.
    IPR027400. S_HR2.
    [Graphical view]
    PfamiPF01600. Corona_S1. 1 hit.
    PF01601. Corona_S2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91AV1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSLIYFWLL LPVLPTLSLP QDVTRCQSTT NFRRFFSKFN VQAPAVVVLG     50
    GYLPSMNSSS WYCGTGIETA SGVHGIFLSY IDSGQGFEIG ISQEPFDPSG 100
    YQLYLHKATN GNTNAIARLR ICQFPDNKTL GPTVNDVTTG RNCLFNKAIP 150
    AYMRDGKDIV VGITWDNDRV TVFADKIYHF YLKNDWSRVA TRCYNRRSCA 200
    MQYVYTPTYY MLNVTSAGED GIYYEPCTAN CTGYAANVFA TDSNGHIPEG 250
    FSFNNWFLLS NDSTLLHGKV VSNQPLLVNC LLAIPKIYGL GQFFSFNHTM 300
    DGVCNGAAVD RAPEALRFNI NDTSVILAEG SIVLHTALGT NLSFVCSNSS 350
    DPHLAIFAIP LGATEVPYYC FLKVDTYNST VYKFLAVLPP TVREIVITKY 400
    GDVYVNGFGY LHLGLLDAVT INFTGHGTDD DVSGFWTIAS TNFVDALIEV 450
    QGTSIQRILY CDDPVSQLKC SQVAFDLDDG FYPISSRNLL SHEQPISFVT 500
    LPSFNDHSFV NITVSAAFGG LSSANLVASD TTINGFSSFC VDTRQFTITL 550
    FYNVTNSYGY VSKSQDSNCP FTLQSVNDYL SFSKFCVSTS LLAGACTIDL 600
    FGYPAFGSGV KLTSLYFQFT KGELITGTPK PLEGITDVSF MTLDVCTKYT 650
    IYGFKGEGII TLTNSSILAG VYYTSDSGQL LAFKNVTSGA VYSVTPCSFS 700
    EQAAYVNDDI VGVISSLSNS TFNNTRELPG FFYHSNDGSN CTEPVLVYSN 750
    IGVCKSGSIG YVPSQYGQVK IAPTVTGNIS IPTNFSMSIR TEYLQLYNTP 800
    VSVDCATYVC NGNSRCKQLL TQYTAACKTI ESALQLSARL ESVEVNSMLT 850
    ISEEALQLAT ISSFNGDGYN FTNVLGASVY DPASGRVVQK RSVIEDLLFN 900
    KVVTNGLGTV DEDYKRCSNG RSVADLVCAQ YYSGVMVLPG VVDAEKLHMY 950
    SASLIGGMAL GGITAAAALP FSYAVQARLN YLALQTDVLQ RNQQLLAESF 1000
    NSAIGNITSA FESVKEAISQ TSKGLNTVAH ALTKVQEVVN SQGSALNQLT 1050
    VQLQHNFQAI SSSIDDIYSR LDILSADVQV DRLITGRLSA LNAFVAQTLT 1100
    KYTEVQASRK LAQQKVNECV KSQSQRYGFC GGDGEHIFSL VQAAPQGLLF 1150
    LHTVLVPGDF VNVLAIAGLC VNGEIALTLR EPGLVLFTHE LQTYTATEYF 1200
    VSSRRMFEPR KPTVSDFVQI ESCVVTYVNL TSDQLPDVIP DYIDVNKTLD 1250
    EILASLPNRT GPSLPLDVFN ATYLNLTGEI ADLEQRSESL RNTTEELRSL 1300
    INNINNTLVD LEWLNRVETY IKWPWWVWLI IVIVLIFVVS LLVFCCISTG 1350
    CCGCCGCCGA CFSGCCRGPR LQPYEAFEKV HVQ 1383
    Length:1,383
    Mass (Da):151,353
    Last modified:December 1, 2001 - v1
    Checksum:i022E5E5E5435876D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF353511 Genomic RNA. Translation: AAK38656.1.
    RefSeqiNP_598310.1. NC_003436.1.

    Genome annotation databases

    GeneIDi935184.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF353511 Genomic RNA. Translation: AAK38656.1 .
    RefSeqi NP_598310.1. NC_003436.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4J79 X-ray 1.56 B 1378-1383 [» ]
    ProteinModelPortali Q91AV1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q91AV1. 3 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 935184.

    Family and domain databases

    Gene3Di 1.20.5.790. 1 hit.
    InterProi IPR002551. Corona_S1.
    IPR002552. Corona_S2.
    IPR027400. S_HR2.
    [Graphical view ]
    Pfami PF01600. Corona_S1. 1 hit.
    PF01601. Corona_S2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF."
      Duarte M., Tobler K., Bridgen A., Rasschaert D., Ackermann M., Laude H.
      Virology 198:466-476(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Further analysis of the genome of porcine epidemic diarrhea virus."
      Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.
      Adv. Exp. Med. Biol. 440:781-786(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence."
      Kocherhans R., Bridgen A., Ackermann M., Tobler K.
      Virus Genes 23:137-144(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiSPIKE_PEDV7
    AccessioniPrimary (citable) accession number: Q91AV1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Caution

    In contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3