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Protein

Spike glycoprotein

Gene

S

Organism
Porcine epidemic diarrhea virus (strain CV777) (PEDV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.UniRule annotation

Caution

In contrast to beta- and gammacoronaviruses, S glycoprotein is not cleaved into S1 and S2.UniRule annotation

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Viral attachment to host cell, Virulence, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spike glycoproteinUniRule annotation
Short name:
S glycoproteinUniRule annotation
Alternative name(s):
E2UniRule annotation
Peplomer proteinUniRule annotation
Gene namesi
Name:SUniRule annotation
ORF Names:2
OrganismiPorcine epidemic diarrhea virus (strain CV777) (PEDV)
Taxonomic identifieri229032 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000008159 Componenti: Genome

Subcellular locationi

  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host endoplasmic reticulum-Golgi intermediate compartment membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 1324Virion surfaceUniRule annotationAdd BLAST1299
Transmembranei1325 – 1344HelicalUniRule annotationAdd BLAST20
Topological domaini1345 – 1383IntravirionUniRule annotationAdd BLAST39

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25UniRule annotationAdd BLAST25
ChainiPRO_000028392526 – 1383Spike glycoproteinUniRule annotationAdd BLAST1358

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ91AV1

Interactioni

Subunit structurei

Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.UniRule annotation

Protein-protein interaction databases

IntActiQ91AV1, 3 interactors
MINTiQ91AV1

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4J79X-ray1.56B1378-1383[»]
ProteinModelPortaliQ91AV1
SMRiQ91AV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 734S1UniRule annotationAdd BLAST709
Regioni617 – 745Interaction with host ANPEPUniRule annotationAdd BLAST129
Regioni735 – 1383S2UniRule annotationAdd BLAST649
Regioni955 – 975Fusion peptideUniRule annotationAdd BLAST21

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1036 – 1080UniRule annotationAdd BLAST45
Coiled coili1272 – 1314UniRule annotationAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1379 – 1383KxHxxUniRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1345 – 1366Cys-richAdd BLAST22

Domaini

The KxHxx motif seems to function as an ER retrieval signal.UniRule annotation

Sequence similaritiesi

Belongs to the alphacoronaviruses spike protein family.UniRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19254
OrthoDBiVOG0900000Z

Family and domain databases

HAMAPiMF_04200 ALPHA_CORONA_SPIKE, 1 hit
InterProiView protein in InterPro
IPR002551 Corona_S1
IPR002552 Corona_S2
PfamiView protein in Pfam
PF01600 Corona_S1, 1 hit
PF01601 Corona_S2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91AV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLIYFWLL LPVLPTLSLP QDVTRCQSTT NFRRFFSKFN VQAPAVVVLG
60 70 80 90 100
GYLPSMNSSS WYCGTGIETA SGVHGIFLSY IDSGQGFEIG ISQEPFDPSG
110 120 130 140 150
YQLYLHKATN GNTNAIARLR ICQFPDNKTL GPTVNDVTTG RNCLFNKAIP
160 170 180 190 200
AYMRDGKDIV VGITWDNDRV TVFADKIYHF YLKNDWSRVA TRCYNRRSCA
210 220 230 240 250
MQYVYTPTYY MLNVTSAGED GIYYEPCTAN CTGYAANVFA TDSNGHIPEG
260 270 280 290 300
FSFNNWFLLS NDSTLLHGKV VSNQPLLVNC LLAIPKIYGL GQFFSFNHTM
310 320 330 340 350
DGVCNGAAVD RAPEALRFNI NDTSVILAEG SIVLHTALGT NLSFVCSNSS
360 370 380 390 400
DPHLAIFAIP LGATEVPYYC FLKVDTYNST VYKFLAVLPP TVREIVITKY
410 420 430 440 450
GDVYVNGFGY LHLGLLDAVT INFTGHGTDD DVSGFWTIAS TNFVDALIEV
460 470 480 490 500
QGTSIQRILY CDDPVSQLKC SQVAFDLDDG FYPISSRNLL SHEQPISFVT
510 520 530 540 550
LPSFNDHSFV NITVSAAFGG LSSANLVASD TTINGFSSFC VDTRQFTITL
560 570 580 590 600
FYNVTNSYGY VSKSQDSNCP FTLQSVNDYL SFSKFCVSTS LLAGACTIDL
610 620 630 640 650
FGYPAFGSGV KLTSLYFQFT KGELITGTPK PLEGITDVSF MTLDVCTKYT
660 670 680 690 700
IYGFKGEGII TLTNSSILAG VYYTSDSGQL LAFKNVTSGA VYSVTPCSFS
710 720 730 740 750
EQAAYVNDDI VGVISSLSNS TFNNTRELPG FFYHSNDGSN CTEPVLVYSN
760 770 780 790 800
IGVCKSGSIG YVPSQYGQVK IAPTVTGNIS IPTNFSMSIR TEYLQLYNTP
810 820 830 840 850
VSVDCATYVC NGNSRCKQLL TQYTAACKTI ESALQLSARL ESVEVNSMLT
860 870 880 890 900
ISEEALQLAT ISSFNGDGYN FTNVLGASVY DPASGRVVQK RSVIEDLLFN
910 920 930 940 950
KVVTNGLGTV DEDYKRCSNG RSVADLVCAQ YYSGVMVLPG VVDAEKLHMY
960 970 980 990 1000
SASLIGGMAL GGITAAAALP FSYAVQARLN YLALQTDVLQ RNQQLLAESF
1010 1020 1030 1040 1050
NSAIGNITSA FESVKEAISQ TSKGLNTVAH ALTKVQEVVN SQGSALNQLT
1060 1070 1080 1090 1100
VQLQHNFQAI SSSIDDIYSR LDILSADVQV DRLITGRLSA LNAFVAQTLT
1110 1120 1130 1140 1150
KYTEVQASRK LAQQKVNECV KSQSQRYGFC GGDGEHIFSL VQAAPQGLLF
1160 1170 1180 1190 1200
LHTVLVPGDF VNVLAIAGLC VNGEIALTLR EPGLVLFTHE LQTYTATEYF
1210 1220 1230 1240 1250
VSSRRMFEPR KPTVSDFVQI ESCVVTYVNL TSDQLPDVIP DYIDVNKTLD
1260 1270 1280 1290 1300
EILASLPNRT GPSLPLDVFN ATYLNLTGEI ADLEQRSESL RNTTEELRSL
1310 1320 1330 1340 1350
INNINNTLVD LEWLNRVETY IKWPWWVWLI IVIVLIFVVS LLVFCCISTG
1360 1370 1380
CCGCCGCCGA CFSGCCRGPR LQPYEAFEKV HVQ
Length:1,383
Mass (Da):151,353
Last modified:December 1, 2001 - v1
Checksum:i022E5E5E5435876D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353511 Genomic RNA Translation: AAK38656.1
RefSeqiNP_598310.1, NC_003436.1

Genome annotation databases

GeneIDi935184
KEGGivg:935184

Similar proteinsi

Entry informationi

Entry nameiSPIKE_PEDV7
AccessioniPrimary (citable) accession number: Q91AV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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