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Q91AV1

- SPIKE_PEDV7

UniProt

Q91AV1 - SPIKE_PEDV7

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Protein

Spike glycoprotein

Gene
S, 2
Organism
Porcine epidemic diarrhea virus (strain CV777) (PEDV)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

S1 region attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes By similarity.

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
  2. pathogenesis Source: UniProtKB-KW
  3. receptor-mediated virion attachment to host cell Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virulence, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spike glycoprotein
Short name:
S glycoprotein
Alternative name(s):
E2
Peplomer protein
Gene namesi
Name:S
ORF Names:2
OrganismiPorcine epidemic diarrhea virus (strain CV777) (PEDV)
Taxonomic identifieri229032 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008159: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein By similarity. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein By similarity
Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Some S oligomers may be transported to the plasma membrane, where they may mediate cell-cell fusion By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 13241306Virion surface Reviewed predictionAdd
BLAST
Transmembranei1325 – 134420Helical; Reviewed predictionAdd
BLAST
Topological domaini1345 – 138339Intravirion Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed predictionAdd
BLAST
Chaini19 – 13831365Spike glycoproteinPRO_0000283925Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi127 – 1271N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi213 – 2131N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi230 – 2301N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi261 – 2611N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi297 – 2971N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi321 – 3211N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi341 – 3411N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi348 – 3481N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi378 – 3781N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi422 – 4221N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi511 – 5111N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi553 – 5531N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi664 – 6641N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi685 – 6851N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi719 – 7191N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi723 – 7231N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi740 – 7401N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi778 – 7781N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi784 – 7841N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi870 – 8701N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1006 – 10061N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1229 – 12291N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1246 – 12461N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1258 – 12581N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1270 – 12701N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1275 – 12751N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1292 – 12921N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi1305 – 13051N-linked (GlcNAc...); by host Reviewed prediction

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotrimer By similarity.

Protein-protein interaction databases

IntActiQ91AV1. 3 interactions.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4J79X-ray1.56B1378-1383[»]
ProteinModelPortaliQ91AV1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 734718S1 By similarityAdd
BLAST
Regioni735 – 1383649S2 By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1036 – 108045 Reviewed predictionAdd
BLAST
Coiled coili1272 – 131443 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1379 – 13835KxHxx By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1345 – 136622Cys-richAdd
BLAST

Domaini

The KxHxx motif seems to function as an ER retrieval signal By similarity.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.5.790. 1 hit.
InterProiIPR002551. Corona_S1.
IPR002552. Corona_S2.
IPR027400. S_HR2.
[Graphical view]
PfamiPF01600. Corona_S1. 1 hit.
PF01601. Corona_S2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91AV1-1 [UniParc]FASTAAdd to Basket

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MRSLIYFWLL LPVLPTLSLP QDVTRCQSTT NFRRFFSKFN VQAPAVVVLG     50
GYLPSMNSSS WYCGTGIETA SGVHGIFLSY IDSGQGFEIG ISQEPFDPSG 100
YQLYLHKATN GNTNAIARLR ICQFPDNKTL GPTVNDVTTG RNCLFNKAIP 150
AYMRDGKDIV VGITWDNDRV TVFADKIYHF YLKNDWSRVA TRCYNRRSCA 200
MQYVYTPTYY MLNVTSAGED GIYYEPCTAN CTGYAANVFA TDSNGHIPEG 250
FSFNNWFLLS NDSTLLHGKV VSNQPLLVNC LLAIPKIYGL GQFFSFNHTM 300
DGVCNGAAVD RAPEALRFNI NDTSVILAEG SIVLHTALGT NLSFVCSNSS 350
DPHLAIFAIP LGATEVPYYC FLKVDTYNST VYKFLAVLPP TVREIVITKY 400
GDVYVNGFGY LHLGLLDAVT INFTGHGTDD DVSGFWTIAS TNFVDALIEV 450
QGTSIQRILY CDDPVSQLKC SQVAFDLDDG FYPISSRNLL SHEQPISFVT 500
LPSFNDHSFV NITVSAAFGG LSSANLVASD TTINGFSSFC VDTRQFTITL 550
FYNVTNSYGY VSKSQDSNCP FTLQSVNDYL SFSKFCVSTS LLAGACTIDL 600
FGYPAFGSGV KLTSLYFQFT KGELITGTPK PLEGITDVSF MTLDVCTKYT 650
IYGFKGEGII TLTNSSILAG VYYTSDSGQL LAFKNVTSGA VYSVTPCSFS 700
EQAAYVNDDI VGVISSLSNS TFNNTRELPG FFYHSNDGSN CTEPVLVYSN 750
IGVCKSGSIG YVPSQYGQVK IAPTVTGNIS IPTNFSMSIR TEYLQLYNTP 800
VSVDCATYVC NGNSRCKQLL TQYTAACKTI ESALQLSARL ESVEVNSMLT 850
ISEEALQLAT ISSFNGDGYN FTNVLGASVY DPASGRVVQK RSVIEDLLFN 900
KVVTNGLGTV DEDYKRCSNG RSVADLVCAQ YYSGVMVLPG VVDAEKLHMY 950
SASLIGGMAL GGITAAAALP FSYAVQARLN YLALQTDVLQ RNQQLLAESF 1000
NSAIGNITSA FESVKEAISQ TSKGLNTVAH ALTKVQEVVN SQGSALNQLT 1050
VQLQHNFQAI SSSIDDIYSR LDILSADVQV DRLITGRLSA LNAFVAQTLT 1100
KYTEVQASRK LAQQKVNECV KSQSQRYGFC GGDGEHIFSL VQAAPQGLLF 1150
LHTVLVPGDF VNVLAIAGLC VNGEIALTLR EPGLVLFTHE LQTYTATEYF 1200
VSSRRMFEPR KPTVSDFVQI ESCVVTYVNL TSDQLPDVIP DYIDVNKTLD 1250
EILASLPNRT GPSLPLDVFN ATYLNLTGEI ADLEQRSESL RNTTEELRSL 1300
INNINNTLVD LEWLNRVETY IKWPWWVWLI IVIVLIFVVS LLVFCCISTG 1350
CCGCCGCCGA CFSGCCRGPR LQPYEAFEKV HVQ 1383
Length:1,383
Mass (Da):151,353
Last modified:December 1, 2001 - v1
Checksum:i022E5E5E5435876D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF353511 Genomic RNA. Translation: AAK38656.1.
RefSeqiNP_598310.1. NC_003436.1.

Genome annotation databases

GeneIDi935184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF353511 Genomic RNA. Translation: AAK38656.1 .
RefSeqi NP_598310.1. NC_003436.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4J79 X-ray 1.56 B 1378-1383 [» ]
ProteinModelPortali Q91AV1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91AV1. 3 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 935184.

Family and domain databases

Gene3Di 1.20.5.790. 1 hit.
InterProi IPR002551. Corona_S1.
IPR002552. Corona_S2.
IPR027400. S_HR2.
[Graphical view ]
Pfami PF01600. Corona_S1. 1 hit.
PF01601. Corona_S2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF."
    Duarte M., Tobler K., Bridgen A., Rasschaert D., Ackermann M., Laude H.
    Virology 198:466-476(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Further analysis of the genome of porcine epidemic diarrhea virus."
    Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.
    Adv. Exp. Med. Biol. 440:781-786(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence."
    Kocherhans R., Bridgen A., Ackermann M., Tobler K.
    Virus Genes 23:137-144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiSPIKE_PEDV7
AccessioniPrimary (citable) accession number: Q91AV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

In contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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