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Q91AV1 (SPIKE_PEDV7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spike glycoprotein

Short name=S glycoprotein
Alternative name(s):
E2
Peplomer protein
Gene names
Name:S
ORF Names:2
OrganismPorcine epidemic diarrhea virus (strain CV777) (PEDV) [Complete proteome]
Taxonomic identifier229032 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length1383 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

S1 region attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes By similarity.

Subunit structure

Homotrimer By similarity.

Subcellular location

Virion membrane; Single-pass type I membrane protein By similarity. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein By similarity. Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Some S oligomers may be transported to the plasma membrane, where they may mediate cell-cell fusion By similarity.

Domain

The KxHxx motif seems to function as an ER retrieval signal By similarity.

Sequence similarities

Belongs to the coronaviruses spike protein family.

Caution

In contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 13831365Spike glycoprotein
PRO_0000283925

Regions

Topological domain19 – 13241306Virion surface Potential
Transmembrane1325 – 134420Helical; Potential
Topological domain1345 – 138339Intravirion Potential
Region17 – 734718S1 By similarity
Region735 – 1383649S2 By similarity
Coiled coil1036 – 108045 Potential
Coiled coil1272 – 131443 Potential
Motif1379 – 13835KxHxx By similarity
Compositional bias1345 – 136622Cys-rich

Amino acid modifications

Glycosylation571N-linked (GlcNAc...); by host Potential
Glycosylation1271N-linked (GlcNAc...); by host Potential
Glycosylation2131N-linked (GlcNAc...); by host Potential
Glycosylation2301N-linked (GlcNAc...); by host Potential
Glycosylation2611N-linked (GlcNAc...); by host Potential
Glycosylation2971N-linked (GlcNAc...); by host Potential
Glycosylation3211N-linked (GlcNAc...); by host Potential
Glycosylation3411N-linked (GlcNAc...); by host Potential
Glycosylation3481N-linked (GlcNAc...); by host Potential
Glycosylation3781N-linked (GlcNAc...); by host Potential
Glycosylation4221N-linked (GlcNAc...); by host Potential
Glycosylation5111N-linked (GlcNAc...); by host Potential
Glycosylation5531N-linked (GlcNAc...); by host Potential
Glycosylation6641N-linked (GlcNAc...); by host Potential
Glycosylation6851N-linked (GlcNAc...); by host Potential
Glycosylation7191N-linked (GlcNAc...); by host Potential
Glycosylation7231N-linked (GlcNAc...); by host Potential
Glycosylation7401N-linked (GlcNAc...); by host Potential
Glycosylation7781N-linked (GlcNAc...); by host Potential
Glycosylation7841N-linked (GlcNAc...); by host Potential
Glycosylation8701N-linked (GlcNAc...); by host Potential
Glycosylation10061N-linked (GlcNAc...); by host Potential
Glycosylation12291N-linked (GlcNAc...); by host Potential
Glycosylation12461N-linked (GlcNAc...); by host Potential
Glycosylation12581N-linked (GlcNAc...); by host Potential
Glycosylation12701N-linked (GlcNAc...); by host Potential
Glycosylation12751N-linked (GlcNAc...); by host Potential
Glycosylation12921N-linked (GlcNAc...); by host Potential
Glycosylation13051N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Q91AV1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 022E5E5E5435876D

FASTA1,383151,353
        10         20         30         40         50         60 
MRSLIYFWLL LPVLPTLSLP QDVTRCQSTT NFRRFFSKFN VQAPAVVVLG GYLPSMNSSS 

        70         80         90        100        110        120 
WYCGTGIETA SGVHGIFLSY IDSGQGFEIG ISQEPFDPSG YQLYLHKATN GNTNAIARLR 

       130        140        150        160        170        180 
ICQFPDNKTL GPTVNDVTTG RNCLFNKAIP AYMRDGKDIV VGITWDNDRV TVFADKIYHF 

       190        200        210        220        230        240 
YLKNDWSRVA TRCYNRRSCA MQYVYTPTYY MLNVTSAGED GIYYEPCTAN CTGYAANVFA 

       250        260        270        280        290        300 
TDSNGHIPEG FSFNNWFLLS NDSTLLHGKV VSNQPLLVNC LLAIPKIYGL GQFFSFNHTM 

       310        320        330        340        350        360 
DGVCNGAAVD RAPEALRFNI NDTSVILAEG SIVLHTALGT NLSFVCSNSS DPHLAIFAIP 

       370        380        390        400        410        420 
LGATEVPYYC FLKVDTYNST VYKFLAVLPP TVREIVITKY GDVYVNGFGY LHLGLLDAVT 

       430        440        450        460        470        480 
INFTGHGTDD DVSGFWTIAS TNFVDALIEV QGTSIQRILY CDDPVSQLKC SQVAFDLDDG 

       490        500        510        520        530        540 
FYPISSRNLL SHEQPISFVT LPSFNDHSFV NITVSAAFGG LSSANLVASD TTINGFSSFC 

       550        560        570        580        590        600 
VDTRQFTITL FYNVTNSYGY VSKSQDSNCP FTLQSVNDYL SFSKFCVSTS LLAGACTIDL 

       610        620        630        640        650        660 
FGYPAFGSGV KLTSLYFQFT KGELITGTPK PLEGITDVSF MTLDVCTKYT IYGFKGEGII 

       670        680        690        700        710        720 
TLTNSSILAG VYYTSDSGQL LAFKNVTSGA VYSVTPCSFS EQAAYVNDDI VGVISSLSNS 

       730        740        750        760        770        780 
TFNNTRELPG FFYHSNDGSN CTEPVLVYSN IGVCKSGSIG YVPSQYGQVK IAPTVTGNIS 

       790        800        810        820        830        840 
IPTNFSMSIR TEYLQLYNTP VSVDCATYVC NGNSRCKQLL TQYTAACKTI ESALQLSARL 

       850        860        870        880        890        900 
ESVEVNSMLT ISEEALQLAT ISSFNGDGYN FTNVLGASVY DPASGRVVQK RSVIEDLLFN 

       910        920        930        940        950        960 
KVVTNGLGTV DEDYKRCSNG RSVADLVCAQ YYSGVMVLPG VVDAEKLHMY SASLIGGMAL 

       970        980        990       1000       1010       1020 
GGITAAAALP FSYAVQARLN YLALQTDVLQ RNQQLLAESF NSAIGNITSA FESVKEAISQ 

      1030       1040       1050       1060       1070       1080 
TSKGLNTVAH ALTKVQEVVN SQGSALNQLT VQLQHNFQAI SSSIDDIYSR LDILSADVQV 

      1090       1100       1110       1120       1130       1140 
DRLITGRLSA LNAFVAQTLT KYTEVQASRK LAQQKVNECV KSQSQRYGFC GGDGEHIFSL 

      1150       1160       1170       1180       1190       1200 
VQAAPQGLLF LHTVLVPGDF VNVLAIAGLC VNGEIALTLR EPGLVLFTHE LQTYTATEYF 

      1210       1220       1230       1240       1250       1260 
VSSRRMFEPR KPTVSDFVQI ESCVVTYVNL TSDQLPDVIP DYIDVNKTLD EILASLPNRT 

      1270       1280       1290       1300       1310       1320 
GPSLPLDVFN ATYLNLTGEI ADLEQRSESL RNTTEELRSL INNINNTLVD LEWLNRVETY 

      1330       1340       1350       1360       1370       1380 
IKWPWWVWLI IVIVLIFVVS LLVFCCISTG CCGCCGCCGA CFSGCCRGPR LQPYEAFEKV 


HVQ 

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References

[1]"Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF."
Duarte M., Tobler K., Bridgen A., Rasschaert D., Ackermann M., Laude H.
Virology 198:466-476(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Further analysis of the genome of porcine epidemic diarrhea virus."
Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.
Adv. Exp. Med. Biol. 440:781-786(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence."
Kocherhans R., Bridgen A., Ackermann M., Tobler K.
Virus Genes 23:137-144(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF353511 Genomic RNA. Translation: AAK38656.1.
RefSeqNP_598310.1. NC_003436.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4J79X-ray1.56B1378-1383[»]
ProteinModelPortalQ91AV1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ91AV1. 3 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID935184.

Family and domain databases

Gene3D1.20.5.790. 1 hit.
InterProIPR002551. Corona_S1.
IPR002552. Corona_S2.
IPR027400. S_HR2.
[Graphical view]
PfamPF01600. Corona_S1. 1 hit.
PF01601. Corona_S2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPIKE_PEDV7
AccessionPrimary (citable) accession number: Q91AV1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references