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Q91989 (P5F13_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 1.3
Alternative name(s):
POU class V protein oct-60
Short name=XOct-60
Short name=XlPOU-60
Gene names
Name:pou5f1.3
Synonyms:oct-60
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription, probably by forming a transcriptional repression complex on the promoters of target genes. Binds to an octamer motif in interspersed RNA. Ref.1 Ref.4 Ref.5 Ref.6

Subunit structure

Interacts with the transcription factors tcf7l1/tcf3 and vegt. Ref.6

Subcellular location

Nucleus By similarity UniProtKB Q01860.

Tissue specificity

Expressed in the animal half of oocytes and remains localized to the animal cap and marginal zone of blastula embryos. Expressed in the adult ovary and at a very low level in the adult kidney. Absent from primordial germ cells (PGCs). Ref.1 Ref.2 Ref.6

Developmental stage

Expressed maternally. Most abundant in oocytes and fertilized eggs, with expression maintained through the cleavage stages of development until early gastrulation. Expression levels drop abruptly during gastrulation (stage 11) but remain at very low levels through larval stages. Ref.1 Ref.2

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426POU domain, class 5, transcription factor 1.3
PRO_0000390934

Regions

Domain202 – 27675POU-specific
DNA binding296 – 35560Homeobox Ref.1 Ref.4
Compositional bias108 – 1136Poly-Ser

Experimental info

Sequence conflict1401A → P in AAA49997. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q91989 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D8C2D596B3BD56C7

FASTA42647,253
        10         20         30         40         50         60 
MDQPILYSQT SFPNFTYSPG VVQDGGNYQY LGNYNAPSYP QPFFHVPVIK SEFGAHEEET 

        70         80         90        100        110        120 
PGSCHAASFD WNLYPHFQIS NQAASNSSGD PSPEGRTEED GSVSEGRSSS SPSPNSPLVP 

       130        140        150        160        170        180 
SFAQYWHYPS WQQGNLTNQA HTLFDGGDEK PQQSRHSPTA SLGSGASNTE DEEVPSAISS 

       190        200        210        220        230        240 
RAERGLCSPS PNNASCGPGT EEDGMTLEEM EEFAKELKQK RVALGYTQGD IGHALGILYG 

       250        260        270        280        290        300 
KMFSQTTICR FESLQLTFKN MCKLKPLLEQ WLGEAENNDN LQEMIHKAQI EEQNRKRKMR 

       310        320        330        340        350        360 
TCFDTVLKGQ LEGHFMCNQK PGARELTEIA KELSLEKDVV RVWFCNRRQK EKSKFRMSKG 

       370        380        390        400        410        420 
HEFVGGASPG SIQSEHISFT PIPANSQDYG LASLHPNRAP FYPPPFPRNE LFPHMAPGIS 


MGVLTG 

« Hide

References

« Hide 'large scale' references
[1]"Sequential expression of multiple POU proteins during amphibian early development."
Hinkley C.S., Martin J.F., Leibham D., Perry M.
Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Gastrula.
[2]"XLPOU-60, a Xenopus POU-domain mRNA, is oocyte-specific from very early stages of oogenesis, and localised to presumptive mesoderm and ectoderm in the blastula."
Whitfield T., Heasman J., Wylie C.
Dev. Biol. 155:361-370(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Oocyte.
[3]NIH - Xenopus Gene Collection (XGC) project
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[4]"Xenopus interspersed RNA families, Ocr and XR, bind DNA-binding proteins."
Guttridge K.L., Smith L.D.
Zygote 3:111-122(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, RNA-BINDING.
[5]"Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
Cao Y., Siegel D., Knochel W.
Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TCF7L1 AND VEGT, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60075 mRNA. Translation: AAA49997.1.
X86377 mRNA. Translation: CAA60136.1.
BC070557 mRNA. Translation: AAH70557.1.
PIRA48829.
RefSeqNP_001081583.1. NM_001088114.1.
UniGeneXl.53382.

3D structure databases

ProteinModelPortalQ91989.
SMRQ91989. Positions 203-353.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397936.
KEGGxla:397936.

Organism-specific databases

CTD397936.
XenbaseXB-GENE-5903594. pou5f3.3.

Phylogenomic databases

HOVERGENHBG053782.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP5F13_XENLA
AccessionPrimary (citable) accession number: Q91989
Secondary accession number(s): Q03916
Entry history
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families