SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q91989

- P5F13_XENLA

UniProt

Q91989 - P5F13_XENLA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

POU domain, class 5, transcription factor 1.3

Gene
pou5f1.3, oct-60
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription, probably by forming a transcriptional repression complex on the promoters of target genes. Binds to an octamer motif in interspersed RNA.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi296 – 35560Homeobox2 PublicationsAdd
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB
  2. sequence-specific DNA binding Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: InterPro
  4. transcription factor binding Source: UniProtKB
  5. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. negative regulation of activin receptor signaling pathway Source: UniProtKB
  4. negative regulation of transcription, DNA-templated Source: UniProtKB
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.3
Alternative name(s):
POU class V protein oct-60
Short name:
XOct-60
Short name:
XlPOU-60
Gene namesi
Name:pou5f1.3
Synonyms:oct-60
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5903594. pou5f3.3.

Subcellular locationi

Nucleus By similarity By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426POU domain, class 5, transcription factor 1.3PRO_0000390934Add
BLAST

Expressioni

Tissue specificityi

Expressed in the animal half of oocytes and remains localized to the animal cap and marginal zone of blastula embryos. Expressed in the adult ovary and at a very low level in the adult kidney. Absent from primordial germ cells (PGCs).3 Publications

Developmental stagei

Expressed maternally. Most abundant in oocytes and fertilized eggs, with expression maintained through the cleavage stages of development until early gastrulation. Expression levels drop abruptly during gastrulation (stage 11) but remain at very low levels through larval stages.2 Publications

Interactioni

Subunit structurei

Interacts with the transcription factors tcf7l1/tcf3 and vegt.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ91989.
SMRiQ91989. Positions 203-353.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 27675POU-specificAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi108 – 1136Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG053782.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91989-1 [UniParc]FASTAAdd to Basket

« Hide

MDQPILYSQT SFPNFTYSPG VVQDGGNYQY LGNYNAPSYP QPFFHVPVIK    50
SEFGAHEEET PGSCHAASFD WNLYPHFQIS NQAASNSSGD PSPEGRTEED 100
GSVSEGRSSS SPSPNSPLVP SFAQYWHYPS WQQGNLTNQA HTLFDGGDEK 150
PQQSRHSPTA SLGSGASNTE DEEVPSAISS RAERGLCSPS PNNASCGPGT 200
EEDGMTLEEM EEFAKELKQK RVALGYTQGD IGHALGILYG KMFSQTTICR 250
FESLQLTFKN MCKLKPLLEQ WLGEAENNDN LQEMIHKAQI EEQNRKRKMR 300
TCFDTVLKGQ LEGHFMCNQK PGARELTEIA KELSLEKDVV RVWFCNRRQK 350
EKSKFRMSKG HEFVGGASPG SIQSEHISFT PIPANSQDYG LASLHPNRAP 400
FYPPPFPRNE LFPHMAPGIS MGVLTG 426
Length:426
Mass (Da):47,253
Last modified:November 1, 1996 - v1
Checksum:iD8C2D596B3BD56C7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401A → P in AAA49997. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60075 mRNA. Translation: AAA49997.1.
X86377 mRNA. Translation: CAA60136.1.
BC070557 mRNA. Translation: AAH70557.1.
PIRiA48829.
RefSeqiNP_001081583.1. NM_001088114.1.
UniGeneiXl.53382.

Genome annotation databases

GeneIDi397936.
KEGGixla:397936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60075 mRNA. Translation: AAA49997.1 .
X86377 mRNA. Translation: CAA60136.1 .
BC070557 mRNA. Translation: AAH70557.1 .
PIRi A48829.
RefSeqi NP_001081583.1. NM_001088114.1.
UniGenei Xl.53382.

3D structure databases

ProteinModelPortali Q91989.
SMRi Q91989. Positions 203-353.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397936.
KEGGi xla:397936.

Organism-specific databases

CTDi 397936.
Xenbasei XB-GENE-5903594. pou5f3.3.

Phylogenomic databases

HOVERGENi HBG053782.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequential expression of multiple POU proteins during amphibian early development."
    Hinkley C.S., Martin J.F., Leibham D., Perry M.
    Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Gastrula.
  2. "XLPOU-60, a Xenopus POU-domain mRNA, is oocyte-specific from very early stages of oogenesis, and localised to presumptive mesoderm and ectoderm in the blastula."
    Whitfield T., Heasman J., Wylie C.
    Dev. Biol. 155:361-370(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Oocyte.
  3. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Xenopus interspersed RNA families, Ocr and XR, bind DNA-binding proteins."
    Guttridge K.L., Smith L.D.
    Zygote 3:111-122(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, RNA-BINDING.
  5. "Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
    Cao Y., Siegel D., Knochel W.
    Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
    Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
    EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TCF7L1 AND VEGT, TISSUE SPECIFICITY.

Entry informationi

Entry nameiP5F13_XENLA
AccessioniPrimary (citable) accession number: Q91989
Secondary accession number(s): Q03916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi