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Q91989

- P5F13_XENLA

UniProt

Q91989 - P5F13_XENLA

Protein

POU domain, class 5, transcription factor 1.3

Gene

pou5f1.3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription, probably by forming a transcriptional repression complex on the promoters of target genes. Binds to an octamer motif in interspersed RNA.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi296 – 35560HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB
    2. sequence-specific DNA binding Source: UniProtKB
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. transcription factor binding Source: UniProtKB
    5. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. negative regulation of activin receptor signaling pathway Source: UniProtKB
    4. negative regulation of transcription, DNA-templated Source: UniProtKB
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1.3
    Alternative name(s):
    POU class V protein oct-601 Publication
    Short name:
    XOct-601 Publication
    Short name:
    XlPOU-60
    Gene namesi
    Name:pou5f1.3
    Synonyms:oct-60Imported
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-5903594. pou5f3.3.

    Subcellular locationi

    Nucleus By similarityPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 426426POU domain, class 5, transcription factor 1.3PRO_0000390934Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in the animal half of oocytes and remains localized to the animal cap and marginal zone of blastula embryos. Expressed in the adult ovary and at a very low level in the adult kidney. Absent from primordial germ cells (PGCs).3 Publications

    Developmental stagei

    Expressed maternally. Most abundant in oocytes and fertilized eggs, with expression maintained through the cleavage stages of development until early gastrulation. Expression levels drop abruptly during gastrulation (stage 11) but remain at very low levels through larval stages.2 Publications

    Interactioni

    Subunit structurei

    Interacts with the transcription factors tcf7l1/tcf3 and vegt.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ91989.
    SMRiQ91989. Positions 203-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini202 – 27675POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi108 – 1136Poly-SerSequence Analysis

    Sequence similaritiesi

    Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    HOVERGENiHBG053782.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91989-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQPILYSQT SFPNFTYSPG VVQDGGNYQY LGNYNAPSYP QPFFHVPVIK    50
    SEFGAHEEET PGSCHAASFD WNLYPHFQIS NQAASNSSGD PSPEGRTEED 100
    GSVSEGRSSS SPSPNSPLVP SFAQYWHYPS WQQGNLTNQA HTLFDGGDEK 150
    PQQSRHSPTA SLGSGASNTE DEEVPSAISS RAERGLCSPS PNNASCGPGT 200
    EEDGMTLEEM EEFAKELKQK RVALGYTQGD IGHALGILYG KMFSQTTICR 250
    FESLQLTFKN MCKLKPLLEQ WLGEAENNDN LQEMIHKAQI EEQNRKRKMR 300
    TCFDTVLKGQ LEGHFMCNQK PGARELTEIA KELSLEKDVV RVWFCNRRQK 350
    EKSKFRMSKG HEFVGGASPG SIQSEHISFT PIPANSQDYG LASLHPNRAP 400
    FYPPPFPRNE LFPHMAPGIS MGVLTG 426
    Length:426
    Mass (Da):47,253
    Last modified:November 1, 1996 - v1
    Checksum:iD8C2D596B3BD56C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti140 – 1401A → P in AAA49997. (PubMed:1732736)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60075 mRNA. Translation: AAA49997.1.
    X86377 mRNA. Translation: CAA60136.1.
    BC070557 mRNA. Translation: AAH70557.1.
    PIRiA48829.
    RefSeqiNP_001081583.1. NM_001088114.1.
    UniGeneiXl.53382.

    Genome annotation databases

    GeneIDi397936.
    KEGGixla:397936.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60075 mRNA. Translation: AAA49997.1 .
    X86377 mRNA. Translation: CAA60136.1 .
    BC070557 mRNA. Translation: AAH70557.1 .
    PIRi A48829.
    RefSeqi NP_001081583.1. NM_001088114.1.
    UniGenei Xl.53382.

    3D structure databases

    ProteinModelPortali Q91989.
    SMRi Q91989. Positions 203-353.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397936.
    KEGGi xla:397936.

    Organism-specific databases

    CTDi 397936.
    Xenbasei XB-GENE-5903594. pou5f3.3.

    Phylogenomic databases

    HOVERGENi HBG053782.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequential expression of multiple POU proteins during amphibian early development."
      Hinkley C.S., Martin J.F., Leibham D., Perry M.
      Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Gastrula1 Publication.
    2. "XLPOU-60, a Xenopus POU-domain mRNA, is oocyte-specific from very early stages of oogenesis, and localised to presumptive mesoderm and ectoderm in the blastula."
      Whitfield T., Heasman J., Wylie C.
      Dev. Biol. 155:361-370(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Oocyte1 Publication.
    3. NIH - Xenopus Gene Collection (XGC) project
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: KidneyImported.
    4. "Xenopus interspersed RNA families, Ocr and XR, bind DNA-binding proteins."
      Guttridge K.L., Smith L.D.
      Zygote 3:111-122(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, RNA-BINDING.
    5. "Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
      Cao Y., Siegel D., Knochel W.
      Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
      Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
      EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TCF7L1 AND VEGT, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiP5F13_XENLA
    AccessioniPrimary (citable) accession number: Q91989
    Secondary accession number(s): Q03916
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3