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Protein

POU domain, class 5, transcription factor 1.3

Gene

pou5f1.3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Antagonizes the activity of nodal/activin signaling during gastrulation to suppress mesendoderm formation. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription, probably by forming a transcriptional repression complex on the promoters of target genes. Binds to an octamer motif in interspersed RNA.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi296 – 355HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: InterPro
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of activin receptor signaling pathway Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.3
Alternative name(s):
POU class V protein oct-601 Publication
Short name:
XOct-601 Publication
Short name:
XlPOU-60
Gene namesi
Name:pou5f1.3
Synonyms:oct-60Imported
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5903594. pou5f3.3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003909341 – 426POU domain, class 5, transcription factor 1.3Add BLAST426

Expressioni

Tissue specificityi

Expressed in the animal half of oocytes and remains localized to the animal cap and marginal zone of blastula embryos. Expressed in the adult ovary and at a very low level in the adult kidney. Absent from primordial germ cells (PGCs).3 Publications

Developmental stagei

Expressed maternally. Most abundant in oocytes and fertilized eggs, with expression maintained through the cleavage stages of development until early gastrulation. Expression levels drop abruptly during gastrulation (stage 11) but remain at very low levels through larval stages.2 Publications

Interactioni

Subunit structurei

Interacts with the transcription factors tcf7l1/tcf3 and vegt.1 Publication

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Structurei

3D structure databases

ProteinModelPortaliQ91989.
SMRiQ91989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini202 – 276POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi108 – 113Poly-SerSequence analysis6

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-5 subfamily.Sequence analysis
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG053782.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR015585. POU_dom_5.
[Graphical view]
PANTHERiPTHR11636:SF79. PTHR11636:SF79. 2 hits.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQPILYSQT SFPNFTYSPG VVQDGGNYQY LGNYNAPSYP QPFFHVPVIK
60 70 80 90 100
SEFGAHEEET PGSCHAASFD WNLYPHFQIS NQAASNSSGD PSPEGRTEED
110 120 130 140 150
GSVSEGRSSS SPSPNSPLVP SFAQYWHYPS WQQGNLTNQA HTLFDGGDEK
160 170 180 190 200
PQQSRHSPTA SLGSGASNTE DEEVPSAISS RAERGLCSPS PNNASCGPGT
210 220 230 240 250
EEDGMTLEEM EEFAKELKQK RVALGYTQGD IGHALGILYG KMFSQTTICR
260 270 280 290 300
FESLQLTFKN MCKLKPLLEQ WLGEAENNDN LQEMIHKAQI EEQNRKRKMR
310 320 330 340 350
TCFDTVLKGQ LEGHFMCNQK PGARELTEIA KELSLEKDVV RVWFCNRRQK
360 370 380 390 400
EKSKFRMSKG HEFVGGASPG SIQSEHISFT PIPANSQDYG LASLHPNRAP
410 420
FYPPPFPRNE LFPHMAPGIS MGVLTG
Length:426
Mass (Da):47,253
Last modified:November 1, 1996 - v1
Checksum:iD8C2D596B3BD56C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140A → P in AAA49997 (PubMed:1732736).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60075 mRNA. Translation: AAA49997.1.
X86377 mRNA. Translation: CAA60136.1.
BC070557 mRNA. Translation: AAH70557.1.
PIRiA48829.
RefSeqiNP_001081583.1. NM_001088114.1.
UniGeneiXl.53382.

Genome annotation databases

GeneIDi397936.
KEGGixla:397936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60075 mRNA. Translation: AAA49997.1.
X86377 mRNA. Translation: CAA60136.1.
BC070557 mRNA. Translation: AAH70557.1.
PIRiA48829.
RefSeqiNP_001081583.1. NM_001088114.1.
UniGeneiXl.53382.

3D structure databases

ProteinModelPortaliQ91989.
SMRiQ91989.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397936.
KEGGixla:397936.

Organism-specific databases

CTDi397936.
XenbaseiXB-GENE-5903594. pou5f3.3.

Phylogenomic databases

HOVERGENiHBG053782.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR015585. POU_dom_5.
[Graphical view]
PANTHERiPTHR11636:SF79. PTHR11636:SF79. 2 hits.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP5F13_XENLA
AccessioniPrimary (citable) accession number: Q91989
Secondary accession number(s): Q03916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.