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Protein

Mast/stem cell growth factor receptor-related protein Kit

Gene

kit

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine kitlg/scf and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis.By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi556 – 5561MagnesiumBy similarity
Binding sitei611 – 6111ATPPROSITE-ProRule annotation
Active sitei776 – 7761Proton acceptorPROSITE-ProRule annotation
Binding sitei780 – 7801ATPPROSITE-ProRule annotation
Metal bindingi781 – 7811MagnesiumBy similarity
Metal bindingi794 – 7941MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi584 – 5918ATPPROSITE-ProRule annotation
Nucleotide bindingi659 – 6657ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mast/stem cell growth factor receptor-related protein Kit (EC:2.7.10.1)
Alternative name(s):
Kit-related kinase 1
Short name:
xKrk1
Tyrosine-protein kinase kit
Gene namesi
Name:kit
Synonyms:krk1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6253062. kit.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 512493ExtracellularSequence analysisAdd
BLAST
Transmembranei513 – 53321HelicalSequence analysisAdd
BLAST
Topological domaini534 – 954421CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 954935Mast/stem cell growth factor receptor-related protein KitPRO_0000248274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 93PROSITE-ProRule annotation
Glycosylationi70 – 701N-linked (GlcNAc...)Sequence analysis
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Disulfide bondi133 ↔ 181PROSITE-ProRule annotation
Disulfide bondi147 ↔ 178PROSITE-ProRule annotation
Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence analysis
Disulfide bondi228 ↔ 281PROSITE-ProRule annotation
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence analysis
Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence analysis
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence analysis
Glycosylationi311 – 3111N-linked (GlcNAc...)Sequence analysis
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence analysis
Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence analysis
Disulfide bondi419 ↔ 483PROSITE-ProRule annotation
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence analysis
Glycosylationi478 – 4781N-linked (GlcNAc...)Sequence analysis
Modified residuei556 – 5561Phosphotyrosine; by autocatalysisBy similarity
Modified residuei558 – 5581Phosphotyrosine; by autocatalysisBy similarity
Modified residuei691 – 6911Phosphotyrosine; by autocatalysisBy similarity
Modified residuei708 – 7081Phosphotyrosine; by autocatalysisBy similarity
Modified residuei807 – 8071Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Ubiquitinated. Rapidly ubiquitinated after autophosphorylation induced by kitlg/scf binding, leading to internalization and degradation.By similarity
Autophosphorylated on tyrosine residues. Phosphorylated tyrosine residues are important for interaction with specific binding partners (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Expressioni

Tissue specificityi

Expressed in a migratory stem cell population.

Developmental stagei

During embryogenesis, maternal transcripts levels are very low through cleavage and blastula stages. Zygotic transcripts are first detected in the early gastrula at stage 10. Transcripts levels increase through tailbud and tadpole stages. At stages 31/32, expression is seen at the surface of the embryo in a line ventral to the posterior edge of the optic cup. At stages 33/34, two shorts lines of cells exist running ventrally from the anterior edge of the otic vesicle. At stages 35/36, expression is seen in cells near the cement gland and in a patch of cells located posterior to the gill arches, and just beneath or within the inner layer of the epidermis. At stages 37/38, expression forms a ribbon on either side of the embryo. At stages 40, a second line runs caudally along the flank of the embryo. By stage 42, extension occurs and a line is observed ventro-caudally around the anus.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ91909.
SMRiQ91909. Positions 535-915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 10989Ig-like C2-type 1Add
BLAST
Domaini118 – 20083Ig-like C2-type 2Add
BLAST
Domaini207 – 29993Ig-like C2-type 3Add
BLAST
Domaini308 – 40194Ig-like C2-type 4Add
BLAST
Domaini404 – 49996Ig-like C2-type 5Add
BLAST
Domaini577 – 912336Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004335.
KOiK05091.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027263. SCGF_receptor.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016243. Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824. Tyr_kinase_rcpt_3_CS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFiPIRSF500951. SCGF_recepter. 1 hit.
PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMARTiSM00409. IG. 4 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWTYLLMLL FLLPYTGDAV PKINDGEDRV TVNVGDKVSL ECRDAHLVTL
60 70 80 90 100
AFQKSGLMKK PRDLKSRPLN NSETDQFFVI IKADLRHIGR YICTNTETQE
110 120 130 140 150
NTSVSLFVKD PARPFLDIPF IDVTEGADTV GMCFPTDPDM DIAIEKCDGS
160 170 180 190 200
PLPENFTFTT DIEAGITIKT VQLAFDSCYV CSGNKSGTVK KSSTFSIHVK
210 220 230 240 250
PVPKKVPTVF LSKSRQLVKT GEPFEVTCAV LDVFSTVKAQ WLDVKEGVTK
260 270 280 290 300
QANFRSSNVF SYNLTLKSDG VPYSESRTFT CQAENAIGQV NATFTLDVID
310 320 330 340 350
VGYVNLTVLE NTTISVNAGD NLVLKVYIDA YPHPDDGVWT YFNETLLNTS
360 370 380 390 400
DHYVATKDEG NNRYVSELHL IRLKGTEKGV YTFYTTNSDD DASVSFNIQV
410 420 430 440 450
KTRPEILIAE RTSEGTLQCV ATGFPVPAIQ WYFCPGSEQR CTDYPPLSPV
460 470 480 490 500
NEKFIQENSS LGRIVVESTI DVNDLKKNGT VQCVASNEVE SAYSVFSFAI
510 520 530 540 550
KEKLRTHTLF TPLLIGFIAA AGLMCIAVAV LMYKYLQKPK YEIQWKVVEE
560 570 580 590 600
INGNNYVYID PTQLPYDNKW EFPRDRLCFG KILGAGAFGK VVEATAYGLL
610 620 630 640 650
KEDSRLTVAV KMLKPSAHST EREALMSELK VLSYLGHHKN IVNLLGACTV
660 670 680 690 700
GGPTLVITEY CCYGDLLNYL RRKRDSFICP KFEDNSEAAL YKNLLNTRDM
710 720 730 740 750
GCEGMSEYID MKPAVSYVVP TKTDKRRSGS FGDQDVSVSI PEEDDLALDT
760 770 780 790 800
EDLINFSYQV AQGMNFLASK NCIHRDLAAR NILLTHGRIT KICDFGLARD
810 820 830 840 850
IRNDSNYVVK GNARLPVKWM APESIFHCVY TFESDVWSYG ILLWEIFSLG
860 870 880 890 900
SSPYPRIPVD SKFYKMIKDG YRMMSPECAP LEMYEIMRSC WNSDPLKRPT
910 920 930 940 950
FKQIVQMVEQ QLSDSKGNTP LPYPVSHVPL DHAVRINSVG SSTSSQPLLT

NSDR
Length:954
Mass (Da):106,860
Last modified:November 1, 1996 - v1
Checksum:i9748845CBE0B537D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48770 mRNA. Translation: CAA88688.1.
PIRiI51703.
RefSeqiNP_001079048.1. NM_001085579.1.
UniGeneiXl.30.

Genome annotation databases

GeneIDi373579.
KEGGixla:373579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48770 mRNA. Translation: CAA88688.1.
PIRiI51703.
RefSeqiNP_001079048.1. NM_001085579.1.
UniGeneiXl.30.

3D structure databases

ProteinModelPortaliQ91909.
SMRiQ91909. Positions 535-915.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi373579.
KEGGixla:373579.

Organism-specific databases

CTDi373579.
XenbaseiXB-GENE-6253062. kit.

Phylogenomic databases

HOVERGENiHBG004335.
KOiK05091.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027263. SCGF_receptor.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016243. Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824. Tyr_kinase_rcpt_3_CS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFiPIRSF500951. SCGF_recepter. 1 hit.
PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMARTiSM00409. IG. 4 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIT_XENLA
AccessioniPrimary (citable) accession number: Q91909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.