Reviewed, UniProtKB/Swiss-Prot Q91845 (EPA4A_XENLA)

Names and origin

Protein names

Recommended name:
    Ephrin type-A receptor 4-A
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor SEK-1
      Short name=xSEK-1

Gene names

Name:	epha4-A
Synonyms:	sek1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	986 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-A family. Required for interactions that regulate the segmental identity or movement of cells By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Developmental stage	Expression occurs in R3, R5 and transiently at lower levels in R2.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 986	966	Ephrin type-A receptor 4-A		PRO_0000016810
Regions
	Topological domain	21 – 547	527	Extracellular Potential
	Transmembrane	548 – 569	22	Potential
	Topological domain	570 – 986	417	Cytoplasmic Potential
	Domain	328 – 430	103	Fibronectin type-III 1
	Domain	440 – 533	94	Fibronectin type-III 2
	Domain	620 – 881	262	Protein kinase
	Domain	911 – 975	65	SAM
	Nucleotide binding	626 – 634	9	ATP By similarity
	Motif	984 – 986	3	PDZ-binding Potential
	Compositional bias	191 – 325	135	Cys-rich
Sites
	Active site	745	1	Proton acceptor By similarity
	Binding site	652	1	ATP By similarity
Amino acid modifications
	Modified residue	595	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	601	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	778	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	928	1	Phosphotyrosine; by autocatalysis Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	407	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q91845-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C34F4078DB3F025F	FASTA	986	109,841
	10 20 30 40 50 60 MAGIVHGILF CGLFGLCWAV TGSRIYPASE VTLLDSRSVQ GELGWIASPL EGGWEEVSIM 70 80 90 100 110 120 DEKNTPIRTY QVCNVMESSQ NNWLRTDWIP RSGAQRVYVE IKFTLRDCNS LPGVMGTCKE 130 140 150 160 170 180 TFNLYYYESN NDKERFIRET QYVKIDTIAA DESFTQVDIG DRIMKLNTEV RDVGPLSKKG 190 200 210 220 230 240 FYLAFQDVGA CIALVSVRVF YKKCPLTVRN LAQFPDTITG SDTSSLVEVR GSCVDNSEEK 250 260 270 280 290 300 DVPKMYCGAD GEWLVPIGNC LCNAGFEEHN GGCQACKVGY YKALSTDAAC SKCPPHSYAL 310 320 330 340 350 360 REGSTSCTCD RGYFRADTDP ASMPCTRPPS APQNLISNVN ETSVNLEWSP PQNSGGRPDV 370 380 390 400 410 420 SYNLVCKRCG SDLTRCRPCG SGVHYSPQQN GLKTTKVSIT DLQAHTNYTF EVWSINGVSK 430 440 450 460 470 480 QNPGQDQAVS VTVTTNQAAP STVTQIQPKD ITRHSVSLTW PEPERPNGVI LEYEVKYYEK 490 500 510 520 530 540 DQNERTYRIV KTTSRSADIK GLNPLTAYVF HVRARTAAGY GEFSGPFEFT TNTVPSPMIG 550 560 570 580 590 600 EGASPTVLLV SVAGSIVLVV ILIAAFVISR RRSKYSKAKQ EADEEKHLNQ GVKTYVDPFT 610 620 630 640 650 660 YEDPNQAVRE FAKEIDASCI KIEKVIGVGE FGEVCSGRLK VPGKREIYVA IKTLKAGYTD 670 680 690 700 710 720 KQRRDFLSEA SIMGQFDHPN IIHLEGVVTK CKPVMIITEY MENGSLDAFL RKNDGRFTVI 730 740 750 760 770 780 QLVGILRGIG SGMKYLSDMS YVHRDLAARN ILVNSNLVCK VSDFGMSRVL EDDPEAAYTT 790 800 810 820 830 840 RGGKIPIRWT APEAIAYRKF TSASDVWSYG IVMWEVMSYG ERPYWDMSNQ DVIKAIEEGY 850 860 870 880 890 900 RLPPPMDCPI ALHQLMLDCW QKERSDRPKF GQIVSMLDKL IRNPNSLKRT GLDNSSRTNT 910 920 930 940 950 960 TLLDPSSPEW SQVASVLDWL QAIKMERYKD NFTAAGYTSL EAVVHVNQDD LTRIGISSPS 970 980 HQNKILSSVQ GMRTQMQQIQ GRMVPV

References

[1]	"Expression of truncated Sek-1 receptor tyrosine kinase disrupts the segmental restriction of gene expression in the Xenopus and zebrafish hindbrain." Xu Q., Alldus G., Holder N., Wilkinson D.G. Development 121:4005-4016(1995) [PubMed: 8575301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91191 mRNA. Translation: CAA62601.1.
RefSeq	NP_001090183.1.
UniGene	Xl.57159
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	779062.
KEGG	xla:779062.
Organism-specific databases
CTD	779062.
Phylogenomic databases
HOVERGEN	Q91845.
Enzyme and pathway databases
BRENDA	2.7.10.1. 648.
Family and domain databases
InterPro	IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR011510. SAM_2. IPR010993. SAM_homology. IPR013761. SAM_type. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR016257. Tyr_prot_kinase_ephrin_rcpt. IPR001426. Tyr_prot_kinase_rcpt_V_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07647. SAM_2. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII.
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPA4A_XENLA

Accession

Primary (citable) accession number: Q91845

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project