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Q91820 (AURAA_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aurora kinase A-A

EC=2.7.11.1
Alternative name(s):
Aurora/IPL1-related kinase 1
Short name=ARK-1
Short name=Aurora-related kinase 1
Serine/threonine-protein kinase 6-A
Serine/threonine-protein kinase Eg2-A
Short name=pEg2
Serine/threonine-protein kinase aurora-A
p46Eg265
Gene names
Name:aurka-a
Synonyms:aurka, eg2, stk6
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Phosphorylates numerous target proteins. Important for microtubule formation and/or stabilization. Ref.1 Ref.3 Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1 Ref.3

Subunit structure

Interacts with kif2c and kif11. Ref.3 Ref.4

Subcellular location

Cytoplasmcytoskeletonspindle. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Note: Localizes to the spindle pole during mitosis especially from prophase through anaphase. Partially colocalized with gamma tubulin in the centrosome, from S to M phase. Ref.1 Ref.3

Tissue specificity

Highly expressed in ovary and testis. Ref.1

Developmental stage

Expressed maternally in oocytes, unfertilized eggs and embryos up to the mid-blastula transition (MBT). Ref.1

Post-translational modification

Phosphorylated. Autophosphorylated on a serine residue. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Aurora kinase A-A
PRO_0000086695

Regions

Domain140 – 390251Protein kinase
Nucleotide binding217 – 2204ATP By similarity
Region287 – 30014Activation segment By similarity

Sites

Active site2631Proton acceptor By similarity
Binding site1501ATP; via amide nitrogen By similarity
Binding site1691ATP Probable
Binding site2811ATP By similarity

Experimental info

Mutagenesis1691K → R: Lacks kinase activity. Disrupts mitotic spindle assembly. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q91820 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DE1628A2C6D11277

FASTA40746,372
        10         20         30         40         50         60 
MERAVKENHK PSNVKIFHPM TEGAKRIPVN QPQSTQFRPP GTAVSAQRIL GPSNVPQRVL 

        70         80         90        100        110        120 
AQAQKPILSS QKPTTQIPLR PATQGHQSSK PQGPNENRNP QQTSHSSTPN VEKKGSTDQG 

       130        140        150        160        170        180 
KTSAVPKEEG KKKQWCLEDF EIGRPLGKGK FGNVYLARER ESKFILALKV LFKSQLEKAG 

       190        200        210        220        230        240 
VEHQLRREVE IQSHLRHPNI LRLYGYFHDA SRVYLILDYA PGGELFRELQ KCTRFDDQRS 

       250        260        270        280        290        300 
AMYIKQLAEA LLYCHSKKVI HRDIKPENLL LGSNGELKIA DFGWSVHAPS SRRTTLCGTL 

       310        320        330        340        350        360 
DYLPPEMIEG RMHDETVDLW SLGVLCYEFL VGKPPFETDT HQETYRRISK VEFQYPPYVS 

       370        380        390        400 
EEARDLVSKL LKHNPNHRLP LKGVLEHPWI IKNSQLKKKD EPLPGAQ 

« Hide

References

« Hide 'large scale' references
[1]"The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly."
Roghi C., Giet R., Uzbekov R., Morin N., Chartrain I., Le Guellec R., Couturier A., Doree M., Phillippe M., Prigent C.
J. Cell Sci. 111:557-572(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AUTOPHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-169.
Tissue: Egg.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[3]"The Xenopus laevis aurora-related protein kinase pEg2 associates with and phosphorylates the kinesin-related protein XlEg5."
Giet R., Uzbekov R., Cubizolles F., Le Guellec K., Prigent C.
J. Biol. Chem. 274:15005-15013(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH KIF11, SUBCELLULAR LOCATION.
[4]"Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity."
Zhang X., Ems-McClung S.C., Walczak C.E.
Mol. Biol. Cell 19:2752-2765(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH KIF2C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z17207 mRNA. Translation: CAA78915.1.
BC072133 mRNA. Translation: AAH72133.1.
PIRS52243.
RefSeqNP_001081565.1. NM_001088096.1.
UniGeneXl.124.

3D structure databases

ProteinModelPortalQ91820.
SMRQ91820. Positions 131-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid99261. 1 interaction.
IntActQ91820. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397925.
KEGGxla:397925.

Organism-specific databases

CTD6790.
XenbaseXB-GENE-866460. aurka.

Phylogenomic databases

HOVERGENHBG108519.
KOK11481.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAURAA_XENLA
AccessionPrimary (citable) accession number: Q91820
Secondary accession number(s): Q6INY9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families