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Protein

Aurora kinase A-A

Gene

aurka-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Phosphorylates numerous target proteins. Important for microtubule formation and/or stabilization.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei150 – 1501ATP; via amide nitrogenPROSITE-ProRule annotation
Binding sitei169 – 1691ATPCurated
Active sitei263 – 2631Proton acceptorPROSITE-ProRule annotation
Binding sitei281 – 2811ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi217 – 2204ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinesin binding Source: UniProtKB
  3. microtubule binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic spindle assembly Source: UniProtKB
  3. peptidyl-serine phosphorylation Source: UniProtKB
  4. protein autophosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aurora kinase A-A (EC:2.7.11.1)
Alternative name(s):
Aurora/IPL1-related kinase 1
Short name:
ARK-1
Short name:
Aurora-related kinase 1
Serine/threonine-protein kinase 6-A
Serine/threonine-protein kinase Eg2-A
Short name:
pEg2
Serine/threonine-protein kinase aurora-A
p46Eg265
Gene namesi
Name:aurka-a
Synonyms:aurka, eg2, stk6
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-866460. aurka.

Subcellular locationi

  1. Cytoplasmcytoskeletonspindle
  2. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome

  3. Note: Localizes to the spindle pole during mitosis especially from prophase through anaphase. Partially colocalized with gamma tubulin in the centrosome, from S to M phase.

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. spindle microtubule Source: UniProtKB
  4. spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi169 – 1691K → R: Lacks kinase activity. Disrupts mitotic spindle assembly. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Aurora kinase A-APRO_0000086695Add
BLAST

Post-translational modificationi

Phosphorylated. Autophosphorylated on a serine residue.

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Highly expressed in ovary and testis.1 Publication

Developmental stagei

Expressed maternally in oocytes, unfertilized eggs and embryos up to the mid-blastula transition (MBT).1 Publication

Interactioni

Subunit structurei

Interacts with kif2c and kif11.2 Publications

Protein-protein interaction databases

BioGridi99261. 1 interaction.
IntActiQ91820. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ91820.
SMRiQ91820. Positions 131-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini140 – 390251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni287 – 30014Activation segmentBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG108519.
KOiK11481.

Family and domain databases

InterProiIPR030616. Aur.
IPR030611. AURKA.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PTHR24350:SF5. PTHR24350:SF5. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERAVKENHK PSNVKIFHPM TEGAKRIPVN QPQSTQFRPP GTAVSAQRIL
60 70 80 90 100
GPSNVPQRVL AQAQKPILSS QKPTTQIPLR PATQGHQSSK PQGPNENRNP
110 120 130 140 150
QQTSHSSTPN VEKKGSTDQG KTSAVPKEEG KKKQWCLEDF EIGRPLGKGK
160 170 180 190 200
FGNVYLARER ESKFILALKV LFKSQLEKAG VEHQLRREVE IQSHLRHPNI
210 220 230 240 250
LRLYGYFHDA SRVYLILDYA PGGELFRELQ KCTRFDDQRS AMYIKQLAEA
260 270 280 290 300
LLYCHSKKVI HRDIKPENLL LGSNGELKIA DFGWSVHAPS SRRTTLCGTL
310 320 330 340 350
DYLPPEMIEG RMHDETVDLW SLGVLCYEFL VGKPPFETDT HQETYRRISK
360 370 380 390 400
VEFQYPPYVS EEARDLVSKL LKHNPNHRLP LKGVLEHPWI IKNSQLKKKD

EPLPGAQ
Length:407
Mass (Da):46,372
Last modified:November 1, 1996 - v1
Checksum:iDE1628A2C6D11277
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z17207 mRNA. Translation: CAA78915.1.
BC072133 mRNA. Translation: AAH72133.1.
PIRiS52243.
RefSeqiNP_001081565.1. NM_001088096.1.
UniGeneiXl.124.

Genome annotation databases

GeneIDi397925.
KEGGixla:397925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z17207 mRNA. Translation: CAA78915.1.
BC072133 mRNA. Translation: AAH72133.1.
PIRiS52243.
RefSeqiNP_001081565.1. NM_001088096.1.
UniGeneiXl.124.

3D structure databases

ProteinModelPortaliQ91820.
SMRiQ91820. Positions 131-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi99261. 1 interaction.
IntActiQ91820. 2 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397925.
KEGGixla:397925.

Organism-specific databases

CTDi6790.
XenbaseiXB-GENE-866460. aurka.

Phylogenomic databases

HOVERGENiHBG108519.
KOiK11481.

Family and domain databases

InterProiIPR030616. Aur.
IPR030611. AURKA.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PTHR24350:SF5. PTHR24350:SF5. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly."
    Roghi C., Giet R., Uzbekov R., Morin N., Chartrain I., Le Guellec R., Couturier A., Doree M., Phillippe M., Prigent C.
    J. Cell Sci. 111:557-572(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AUTOPHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-169.
    Tissue: Egg.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. "The Xenopus laevis aurora-related protein kinase pEg2 associates with and phosphorylates the kinesin-related protein XlEg5."
    Giet R., Uzbekov R., Cubizolles F., Le Guellec K., Prigent C.
    J. Biol. Chem. 274:15005-15013(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH KIF11, SUBCELLULAR LOCATION.
  4. "Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity."
    Zhang X., Ems-McClung S.C., Walczak C.E.
    Mol. Biol. Cell 19:2752-2765(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KIF2C.

Entry informationi

Entry nameiAURAA_XENLA
AccessioniPrimary (citable) accession number: Q91820
Secondary accession number(s): Q6INY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.