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Q91790

- DUS1A_XENLA

UniProt

Q91790 - DUS1A_XENLA

Protein

Dual specificity protein phosphatase 1-A

Gene

dusp1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-188' and 'Tyr-190', regulating its activity during the meiotic cell cycle.2 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.
    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.2 PublicationsPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei260 – 2601Phosphocysteine intermediateBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: UniProtKB
    2. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. inactivation of MAPK activity Source: UniProtKB
    2. meiotic nuclear division Source: UniProtKB-KW
    3. mitotic cell cycle arrest Source: UniProtKB
    4. negative regulation of MAPK cascade Source: UniProtKB
    5. negative regulation of meiotic cell cycle Source: UniProtKB
    6. peptidyl-threonine dephosphorylation Source: UniProtKB
    7. peptidyl-tyrosine dephosphorylation Source: UniProtKB
    8. regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
    9. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Meiosis, Stress response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 1-A (EC:3.1.3.162 Publications, EC:3.1.3.482 Publications)
    Alternative name(s):
    XCL1001 Publication
    XCL100-alfa1 Publication
    Gene namesi
    Name:dusp1-aImported
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-975062. dusp1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi168 – 1681T → E: Abolishes threonine phosphorylation; when associated with S-260. No effect on phosphatase activity and MAPK1 binding; when associated with S-260. 1 Publication
    Mutagenesisi168 – 1681T → V: Abolishes threonine phosphorylation; when associated with S-260. No effect on phosphatase activity and MAPK1 binding; when associated with S-260. 1 Publication
    Mutagenesisi260 – 2601C → S: Loss of phosphatase activity. Increases stability. Enables stable complex formation with active MAPK1. Abolishes threonine phosphorylation; when associated with E-168 or V-168. No effect on phosphatase activity and MAPK1 binding; when associated with E-168 or V-168. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Dual specificity protein phosphatase 1-APRO_0000421473Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei168 – 1681Phosphothreonine; by MAPK11 Publication

    Post-translational modificationi

    Phosphorylated by MAPK1/ERK2 at Thr-168 and at one or more serine residues in a progesterone-dependent manner. Phosphorylation reduces its rate of degradation but does not seem to affect phosphatase activity.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Expressed in XIK-2 kidney cells.1 Publication

    Developmental stagei

    Present in both immature and mature oocytes (at protein level). Expressed at a constant level during oocyte growth as well as during oocyte maturation. Levels decline somewhat during cleavage (stages 3-6). Levels rise dramatically during the mid-blastula transition. This higher level of expression is then maintained through gastrulation and all subsequent developmental stages.1 Publication

    Inductioni

    By serum stimulation, heat shock and oxidative stress.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ91790.
    SMRiQ91790. Positions 173-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 138118RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini175 – 369195Tyrosine-protein phosphataseCuratedSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 rhodanese domain.PROSITE-ProRule annotation
    Contains 1 tyrosine-protein phosphatase domain.Sequence Analysis

    Phylogenomic databases

    HOVERGENiHBG007347.
    KOiK04459.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR020417. Atypical_DUSP.
    IPR020420. Atypical_DUSP_famB.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSiPR01908. ADSPHPHTASE.
    PR01910. ADSPHPHTASEB.
    PR01764. MAPKPHPHTASE.
    SMARTiSM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91790-1 [UniParc]FASTAAdd to Basket

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    MVNMETCAMD CCVLKALLAE RAHKCLILDC RSFFSFSSCS IVGSSNVRLS    50
    TIVKRRAKGS MGLEHIVPNE EQRCRLVAGM YEAVVLLDER TSELDMLRKD 100
    STMMLAVNAL CRDSRGSSIY FLKGGYETFS AQCPEFCTKN SPPVGLSLPL 150
    CANNVPGSAD SNCTPCGTPL YDQGGPVEIL PFLYLGSAYH ASRKDMLDTL 200
    GITALINVSA NCPNHFEGHF QYKSIPVEDS HKADISSWFN EAIDFIDSVK 250
    NSGGRVFVHC QAGISRSATI CLAYLMRTNR VKLDEAFEFV KQRRSIISPN 300
    FSFMGQLLQF ESQVLAPSCS AEAGSPTISV LDRGTSTTTV FNFPVSIPVH 350
    SGANSLSYLQ NPITTSPSC 369
    Length:369
    Mass (Da):40,264
    Last modified:November 1, 1996 - v1
    Checksum:i14336CA5EC35AAF7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83742 mRNA. Translation: CAA58710.1.
    AJ320158 mRNA. Translation: CAC44126.1.
    RefSeqiNP_001080570.1. NM_001087101.1.
    UniGeneiXl.1243.

    Genome annotation databases

    GeneIDi380262.
    KEGGixla:380262.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83742 mRNA. Translation: CAA58710.1 .
    AJ320158 mRNA. Translation: CAC44126.1 .
    RefSeqi NP_001080570.1. NM_001087101.1.
    UniGenei Xl.1243.

    3D structure databases

    ProteinModelPortali Q91790.
    SMRi Q91790. Positions 173-315.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 380262.
    KEGGi xla:380262.

    Organism-specific databases

    CTDi 1843.
    Xenbasei XB-GENE-975062. dusp1.

    Phylogenomic databases

    HOVERGENi HBG007347.
    KOi K04459.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR020417. Atypical_DUSP.
    IPR020420. Atypical_DUSP_famB.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSi PR01908. ADSPHPHTASE.
    PR01910. ADSPHPHTASEB.
    PR01764. MAPKPHPHTASE.
    SMARTi SM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "XCL100, an inducible nuclear MAP kinase phosphatase from Xenopus laevis: its role in MAP kinase inactivation in differentiated cells and its expression during early development."
      Lewis T., Groom L.A., Sneddon A.A., Smythe C., Keyse S.M.
      J. Cell Sci. 108:2885-2896(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Tissue: Embryonic kidney1 Publication.
    2. "Activation of p42 mitogen-activated protein kinase (MAPK), but not c-Jun NH(2)-terminal kinase, induces phosphorylation and stabilization of MAPK phosphatase XCL100 in Xenopus oocytes."
      Sohaskey M.L., Ferrell J.E. Jr.
      Mol. Biol. Cell 13:454-468(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PHOSPHORYLATION AT THR-168, PHOSPHORYLATION AT SERINE RESIDUES, MUTAGENESIS OF THR-168 AND CYS-260.
      Tissue: OvaryImported.

    Entry informationi

    Entry nameiDUS1A_XENLA
    AccessioniPrimary (citable) accession number: Q91790
    Secondary accession number(s): Q90W59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2013
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3