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Protein

Homeobox protein not2

Gene

not2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional repressor (By similarity). Plays a fundamental role in notochord formation, acting within the mesodermal region. Acts downstream of gsc and upstream of chrd and foxa4-A/pintallavis.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi135 – 19460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • notochord formation Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein not2
Short name:
Xnot-2
Short name:
Xnot2
Gene namesi
Name:not21 Publication
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252631. not.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Homeobox protein not2PRO_0000274814Add
BLAST

Expressioni

Tissue specificityi

Localized to the dorsal lip of the blastopore (Spemann organizer) during early gastrulation, after which expression continues in tissues derived from the organizer. Expressed in the notochord during mid-gastrulation, the chordoneural hinge, notochord and ventral spinal cord of the tailbud at stage 22, and finally the tip of the tail in the tadpole (stage 35).1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ91770.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG082047.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHSPVFPAF GHHLADYPAM PLATDLPRTP KSSFNIDSIL SRTDRPASKV
60 70 80 90 100
SMEMPSWQPP SPPFHYSYGM VPYPPMWLIK PTVGYPNMAQ AQPMRMPRGE
110 120 130 140 150
CLCPDPACKH RGLSYSHCPN GALNPLSWRT GPCKLKRIRT VFTPEQLERL
160 170 180 190 200
EKEFLKQQYM VGTERVDLAS TLNLTETQVK VWFQNRRIKW RKQSLEQKKA
210 220 230
KLSQFGVILA DSSDHTDDSR ETEEEEDDVD VEL
Length:233
Mass (Da):26,729
Last modified:November 1, 1996 - v1
Checksum:i7EFDD5A4F2BC2156
GO

Sequence cautioni

The sequence AAI23278.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19566 mRNA. Translation: AAA19694.1.
BC123277 mRNA. Translation: AAI23278.1. Different initiation.
PIRiI51438.
RefSeqiNP_001081191.1. NM_001087722.1.
UniGeneiXl.1066.

Genome annotation databases

GeneIDi397703.
KEGGixla:397703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19566 mRNA. Translation: AAA19694.1.
BC123277 mRNA. Translation: AAI23278.1. Different initiation.
PIRiI51438.
RefSeqiNP_001081191.1. NM_001087722.1.
UniGeneiXl.1066.

3D structure databases

ProteinModelPortaliQ91770.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397703.
KEGGixla:397703.

Organism-specific databases

CTDi397703.
XenbaseiXB-GENE-6252631. not.

Phylogenomic databases

HOVERGENiHBG082047.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tail formation as a continuation of gastrulation: the multiple cell populations of the Xenopus tailbud derive from the late blastopore lip."
    Gont L.K., Steinbeisser H., Blumberg B., De Robertis E.M.
    Development 119:991-1004(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Egg1 Publication.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: NeurulaImported.
  3. "Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus."
    Gont L.K., Fainsod A., Kim S.-H., De Robertis E.M.
    Dev. Biol. 174:174-178(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme in Xenopus gastrulae."
    Yasuo H., Lemaire P.
    Development 128:3783-3793(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNOT2_XENLA
AccessioniPrimary (citable) accession number: Q91770
Secondary accession number(s): Q0IH75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.