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Protein

Forkhead box protein A2-A

Gene

foxa2-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator during early development, limiting the extent of mesoderm formation in the gastrula. Binds to DNA via the target sequence 5'-GT[AC]AACA-3', with 5'-GTAAACA-3' being the preferred binding site.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi149 – 24395Fork-headPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • mesoderm formation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein A2-A
Short name:
FoxA2-A
Short name:
FoxA2a
Alternative name(s):
Fork head domain-related protein 3
Short name:
xFD-3
Hepatocyte nuclear factor 3-beta homolog A
Short name:
HNF-3-beta-A
Short name:
HNF3-beta homolog A
Short name:
HNF3-beta-A
Short name:
xHNF3-beta-A
Short name:
xbeta-1
Gene namesi
Name:foxa2-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865381. foxa2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi195 – 1951N → D: Abolishes DNA-binding activity and reduces ability to inhibit mesoderm formation; when associated with C-199 and E-241. 1 Publication
Mutagenesisi199 – 1991H → C: Abolishes DNA-binding activity and reduces ability to inhibit mesoderm formation; when associated with D-195 and E-241. 1 Publication
Mutagenesisi241 – 2411R → E: Abolishes DNA-binding activity and reduces ability to inhibit mesoderm formation; when associated with D-195 and C-199. 1 Publication

Chemistry

ChEMBLiCHEMBL3350224.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Forkhead box protein A2-APRO_0000248856Add
BLAST

Expressioni

Tissue specificityi

At gastrula stage, expressed in both the anterior and posterior endoderm, with endodermal expression persisting into early tailbud stages. Expression is absent in gastrula stage ectoderm. During tailbud stages, expressed in the pharyngeal region, the neural floor plate, the midbrain, hindbrain and in cranial neural crest cells. Expressed in the foregut of hatching larvae. In tadpoles, expressed in the pharyngeal pouches and in other anterior endodermal regions. Within the tadpole nervous system, expressed in the neural floor plate, at high levels in the ventral midbrain and hindbrain, and at lower levels in the spinal cord. Expressed in the adult lung and brain.3 Publications

Developmental stagei

First detected in early gastrula stage embryos. Abundant at the neurula stage, becoming less abundant at later stages.3 Publications

Structurei

3D structure databases

ProteinModelPortaliQ91765.
SMRiQ91765. Positions 149-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 11181Met-richSequence analysisAdd
BLAST
Compositional biasi262 – 33271Ser-richSequence analysisAdd
BLAST
Compositional biasi322 – 37554His-richSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG006621.
KOiK08035.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR013638. Fork-head_N.
IPR001766. Fork_head_dom.
IPR018533. Forkhead_box_C.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
PF08430. Forkhead_N. 1 hit.
PF09354. HNF_C. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGAVKMEGH EATDWSSYYG EAEAYSSVGN MNAGLSMNPM NTYMSMSAMR
60 70 80 90 100
TSANMTASSM NMSYVNTGMS PSLTGMSPGT GAMTGMGTGV PSMASHLSPS
110 120 130 140 150
MIPMSAQTTA MNALAPYTNI NSMSPIYGQS NINRSRDPKT YRRSYTHAKP
160 170 180 190 200
PYSYISLITM AIQQSPNKML TLSEIYQWIM DLFPFYRQNQ QRWQNSIRHS
210 220 230 240 250
LSFNDCFLKV PRSPDKPGKG SFWTLHPDSG NMFENGCYLR RQKRFKCEKK
260 270 280 290 300
PSLREGGGKK LSEGASSVGS AANSSSESSV GNESPHSSSS PCQEQKRSLV
310 320 330 340 350
DMKSSQGLSP KHATSPASQA QHLLSQHHSV LSHEAQSHLK PEHHYSFNHP
360 370 380 390 400
FSINNLMSSE QQHHHHHHHN HHHHHKMDLK AYEQVMHYSS YGSPMAGSLA
410 420 430
MSTVTNKSGL ESSPITSDTS YYQGGYSRPI MNSS
Length:434
Mass (Da):47,986
Last modified:November 1, 1996 - v1
Checksum:iB6C58F40F2D3D705
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501R → S no nucleotide entry (PubMed:8735935).Curated
Sequence conflicti102 – 1021I → S no nucleotide entry (PubMed:8735935).Curated
Sequence conflicti311 – 3111K → E no nucleotide entry (PubMed:8735935).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25637 Genomic DNA. Translation: AAA20679.1.
PIRiI51436.
RefSeqiNP_001165629.1. NM_001172158.1.
UniGeneiXl.40286.

Genome annotation databases

GeneIDi100127318.
KEGGixla:100127318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25637 Genomic DNA. Translation: AAA20679.1.
PIRiI51436.
RefSeqiNP_001165629.1. NM_001172158.1.
UniGeneiXl.40286.

3D structure databases

ProteinModelPortaliQ91765.
SMRiQ91765. Positions 149-239.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL3350224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100127318.
KEGGixla:100127318.

Organism-specific databases

CTDi3170.
XenbaseiXB-GENE-865381. foxa2.

Phylogenomic databases

HOVERGENiHBG006621.
KOiK08035.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR013638. Fork-head_N.
IPR001766. Fork_head_dom.
IPR018533. Forkhead_box_C.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
PF08430. Forkhead_N. 1 hit.
PF09354. HNF_C. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFXA2A_XENLA
AccessioniPrimary (citable) accession number: Q91765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.