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Q91757

- GSK3B_XENLA

UniProt

Q91757 - GSK3B_XENLA

Protein

Glycogen synthase kinase-3 beta

Gene

gsk3b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Plays a role in the organization of the formation of the main body axis of developing embryo. Acts as an inhibitor of differentiation of primary neurons. Inhibits the ability of ectopically expressed NEUROD1 and other bHLH factors to promote early retinal cell differentiation. May participate in the Wnt signaling pathway. May regulate the circadian clock via phosphorylation of the major clock components.4 Publications

    Catalytic activityi

    ATP + [tau protein] = ADP + [tau protein] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851ATPPROSITE-ProRule annotation
    Active sitei181 – 1811Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 709ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB
    3. tau-protein kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. canonical Wnt signaling pathway Source: BHF-UCL
    2. cell differentiation Source: UniProtKB-KW
    3. negative regulation of transcription, DNA-templated Source: BHF-UCL
    4. nervous system development Source: UniProtKB-KW
    5. rhythmic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Biological rhythms, Differentiation, Neurogenesis, Wnt signaling pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen synthase kinase-3 beta (EC:2.7.11.26)
    Short name:
    GSK-3 beta
    Alternative name(s):
    Xgsk-3 protein
    Gene namesi
    Name:gsk3b
    Synonyms:Xgsk-3
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-865674. gsk3b.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Cell membrane By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi85 – 851K → R: Inhibits enzymatic activity. Induces duplication of the anterior dorsal axis. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420Glycogen synthase kinase-3 betaPRO_0000412070Add
    BLAST

    Post-translational modificationi

    Phosphorylated. Activated by phosphorylation at Tyr-216.

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Expressed in the unfertilized egg and throughout the early embryonic stages. Expressed in the neural plate at stage 13. Expressed throughout the eye primordia and cranial ganglia at stage 25. Highly expressed in the differentiated retinal ganglion cell layer at stage 40.3 Publications

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-100944.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91757.
    SMRiQ91757. Positions 28-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 340285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.
    KOiK03083.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91757-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGRPRTTSF AESCKPVQQP SSFGSMKVSR DKDGSKVTTV VATPGQGPDR    50
    QQEVTYTDTK VIGNGSFGVV YQAKLCDTGE LVAIKKVLQD KRFKNRELQI 100
    MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK 150
    QALPIIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PETAVLKLCD 200
    FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL 250
    LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP 300
    WTKVFRARTP PEAIALCSRL LEYTPTSRLT PLDACAHSFF DELRDPNLKL 350
    PNGREFPALF NFTTQELSSN PSLSSILIPA HARNQAAVST TSNTTSTSDS 400
    NTGERGSTNN AASASASNSS 420
    Length:420
    Mass (Da):46,903
    Last modified:November 1, 1996 - v1
    Checksum:iC02280BB8A35785D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti55 – 551T → S in AAA84444. (PubMed:7667318)Curated
    Sequence conflicti88 – 881L → P in AAA84444. (PubMed:7667318)Curated
    Sequence conflicti336 – 3361A → V in AAA84444. (PubMed:7667318)Curated
    Sequence conflicti399 – 3991D → N in AAA84444. (PubMed:7667318)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38492 mRNA. Translation: AAC42224.1.
    U31862 mRNA. Translation: AAA84444.1.
    BC108581 mRNA. Translation: AAI08582.1.
    PIRiI51425.
    I51692.
    RefSeqiNP_001083752.1. NM_001090283.1.
    UniGeneiXl.324.

    Genome annotation databases

    GeneIDi399097.
    KEGGixla:399097.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38492 mRNA. Translation: AAC42224.1 .
    U31862 mRNA. Translation: AAA84444.1 .
    BC108581 mRNA. Translation: AAI08582.1 .
    PIRi I51425.
    I51692.
    RefSeqi NP_001083752.1. NM_001090283.1.
    UniGenei Xl.324.

    3D structure databases

    ProteinModelPortali Q91757.
    SMRi Q91757. Positions 28-383.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-100944.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399097.
    KEGGi xla:399097.

    Organism-specific databases

    CTDi 2932.
    Xenbasei XB-GENE-865674. gsk3b.

    Phylogenomic databases

    HOVERGENi HBG014652.
    KOi K03083.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of Spemann organizer formation by the intracellular kinase Xgsk-3."
      Pierce S.B., Kimelman D.
      Development 121:755-765(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LYS-85, DEVELOPMENTAL STAGE.
    2. "Role of glycogen synthase kinase 3 beta as a negative regulator of dorsoventral axis formation in Xenopus embryos."
      Dominguez I., Itoh K., Sokol S.Y.
      Proc. Natl. Acad. Sci. U.S.A. 92:8498-8502(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LYS-85, DEVELOPMENTAL STAGE.
    3. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.
    4. "The role of GSK3beta in regulating neuronal differentiation in Xenopus laevis."
      Marcus E.A., Kintner C., Harris W.
      Mol. Cell. Neurosci. 12:269-280(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    5. "Posttranslational mechanisms control the timing of bHLH function and regulate retinal cell fate."
      Moore K.B., Schneider M.L., Vetter M.L.
      Neuron 34:183-195(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGSK3B_XENLA
    AccessioniPrimary (citable) accession number: Q91757
    Secondary accession number(s): Q91627
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3