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Protein

Focal adhesion kinase 1

Gene

ptk2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei467ATPPROSITE-ProRule annotation1
Active sitei559Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi441 – 447ATPPROSITE-ProRule annotation7
Nucleotide bindingi513 – 515ATPPROSITE-ProRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
Focal adhesion kinase 1 (EC:2.7.10.2)
Short name:
FADK 1
Alternative name(s):
Protein-tyrosine kinase 2
pp125FAK
Gene namesi
Name:ptk2
Synonyms:fak1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-952029. ptk2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880801 – 1068Focal adhesion kinase 1Add BLAST1068

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei403PhosphotyrosineBy similarity1
Modified residuei413PhosphotyrosineBy similarity1
Modified residuei589Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei590Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei874PhosphotyrosineBy similarity1
Modified residuei941PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues; phosphorylated kinase is first detected during gastrulation, suggesting that tyrosine phosphorylation is developmentally regulated.

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Present in the fertilized egg and in cleavage and blastula stage embryos. During gastrulation, expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, prominently expressed at intersomitic junctions, in the brain and in several cranial nerves.

Structurei

3D structure databases

ProteinModelPortaliQ91738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 355FERMPROSITE-ProRule annotationAdd BLAST321
Domaini435 – 693Protein kinasePROSITE-ProRule annotationAdd BLAST259

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi725 – 746Pro-richAdd BLAST22
Compositional biasi876 – 929Pro-richAdd BLAST54

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.PROSITE-ProRule annotation
Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG004018.
KOiK05725.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR005189. Focal_adhesion_kin_target_dom.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF03623. Focal_AT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
ProDomiPD006413. Focal_adhesion_target_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00295. B41. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF68993. SSF68993. 1 hit.
PROSITEiPS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q91738-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAYLDPNL NHNPSTNAKS RLSTGMERSP GAIERVLRVF HYFESNNEPA
60 70 80 90 100
TWSSNIRHGD ATDVRGIIQK IVDSHKVKNV ASYGLRLSHL HSEEVHWLHP
110 120 130 140 150
DIGVSHIREK YEQSHPPEEW KYELRIRYLP KGFVNQFTED KPTLNFFYQQ
160 170 180 190 200
VKNDYMSEIA DQVDQEIALK LGCLEIRRSY GEMRGNALEK KSNYEVLEKD
210 220 230 240 250
VGLKRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI LKFFEILSPV
260 270 280 290 300
YRYDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGSNP THLADFTQVQ
310 320 330 340 350
TIQYSSSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL
360 370 380 390 400
VSGASESFII RPQKEGERAL PSIPKLANNE KHGVRPHAVS VSDEFSGDET
410 420 430 440 450
DDYAEIIDEE DTYTMPSKSY GLDEAGDYEI QRDRIELGRC IGEGQFGDVH
460 470 480 490 500
QGVYMSPENP AMAVAIKTCK NCTSDSVREK FLQEALTMRQ FDHPHIVKLI
510 520 530 540 550
GVITENPVWI IMELCTLGEL RSFLQVRKYS LDLASLILYS YQLSTALAYL
560 570 580 590 600
ESKRFVHRDI AARNVLVSSS DCVKLGDFGL SRYMEDSTYY KASKGKLPIK
610 620 630 640 650
WMAPESINFR RFTSASDVWM FGVCMWEILM YGVKPFQGVK NNDVIGRIEN
660 670 680 690 700
GERLPMPPNC PPTLYSLMTK CWAYDPSRRP RFTELKAQLS TILEEEKLQQ
710 720 730 740 750
EERMRMESRR QVTVSWDSGG SDEAPPKPSR PGYPSPRSSE GFFPSPQHMM
760 770 780 790 800
QPNHYQVSGF SVAHGIPSMS GNMYPGQASV LDHMDSWNHR TPDINMWQPS
810 820 830 840 850
MEDSGPMDMR SLAQVLPTHL MEERLIRQQQ EMEEDQRWLE KEERFLKPDV
860 870 880 890 900
RLSRGSVDHV DGNIQCPAGN QHIYQPVGKP DHVAPPKKPP RPGAPSHLGN
910 920 930 940 950
LPAHNSPVDG YNEGVKPWRI QPQEISPPPT ANLDRTNDKV YENVTGLVKA
960 970 980 990 1000
VIEMSSRIQP APPEEYVPMV KGVGLALRTL LATVDETIPV LPASTHREIE
1010 1020 1030 1040 1050
MAQKLLNSDL AELINKMKLA QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL
1060
LDVIDQARLK IISHSRPH
Length:1,068
Mass (Da):121,156
Last modified:January 23, 2002 - v2
Checksum:iEF5BEBD60D2C99DA
GO
Isoform Short (identifier: Q91738-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-398: Missing.
     418-425: KSYGLDEA → T
     917-919: Missing.

Show »
Length:1,052
Mass (Da):119,303
Checksum:i05A551229F0BCFF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti439R → P in AAA99456 (PubMed:7649362).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004978393 – 398Missing in isoform Short. 1 Publication6
Alternative sequenceiVSP_004979418 – 425KSYGLDEA → T in isoform Short. 1 Publication8
Alternative sequenceiVSP_004980917 – 919Missing in isoform Short. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33920 mRNA. Translation: AAA99456.1.
U11078 mRNA. Translation: AAA80333.1.
PIRiI51670.
JC4200.
RefSeqiNP_001084066.1. NM_001090597.1. [Q91738-1]
UniGeneiXl.6819.

Genome annotation databases

GeneIDi399286.
KEGGixla:399286.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33920 mRNA. Translation: AAA99456.1.
U11078 mRNA. Translation: AAA80333.1.
PIRiI51670.
JC4200.
RefSeqiNP_001084066.1. NM_001090597.1. [Q91738-1]
UniGeneiXl.6819.

3D structure databases

ProteinModelPortaliQ91738.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399286.
KEGGixla:399286.

Organism-specific databases

CTDi5747.
XenbaseiXB-GENE-952029. ptk2.

Phylogenomic databases

HOVERGENiHBG004018.
KOiK05725.

Enzyme and pathway databases

BRENDAi2.7.10.2. 6725.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR005189. Focal_adhesion_kin_target_dom.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF03623. Focal_AT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
ProDomiPD006413. Focal_adhesion_target_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00295. B41. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF68993. SSF68993. 1 hit.
PROSITEiPS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFAK1_XENLA
AccessioniPrimary (citable) accession number: Q91738
Secondary accession number(s): Q91563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2002
Last modified: October 5, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.