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Q91738

- FAK1_XENLA

UniProt

Q91738 - FAK1_XENLA

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Protein
Focal adhesion kinase 1
Gene
ptk2, fak1
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly By similarity.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei467 – 4671ATP By similarity
Active sitei559 – 5591Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi441 – 4477ATP By similarity
Nucleotide bindingi513 – 5153ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. signal transducer activity Source: InterPro

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. signal complex assembly Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 6726.

Names & Taxonomyi

Protein namesi
Recommended name:
Focal adhesion kinase 1 (EC:2.7.10.2)
Short name:
FADK 1
Alternative name(s):
Protein-tyrosine kinase 2
pp125FAK
Gene namesi
Name:ptk2
Synonyms:fak1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-952029. ptk2.

Subcellular locationi

GO - Cellular componenti

  1. cytoskeleton Source: InterPro
  2. focal adhesion Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10681068Focal adhesion kinase 1
PRO_0000088080Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei403 – 4031Phosphotyrosine By similarity
Modified residuei413 – 4131Phosphotyrosine By similarity
Modified residuei589 – 5891Phosphotyrosine; by autocatalysis By similarity
Modified residuei590 – 5901Phosphotyrosine; by autocatalysis By similarity
Modified residuei874 – 8741Phosphotyrosine By similarity
Modified residuei941 – 9411Phosphotyrosine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues; phosphorylated kinase is first detected during gastrulation, suggesting that tyrosine phosphorylation is developmentally regulated.

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Present in the fertilized egg and in cleavage and blastula stage embryos. During gastrulation, expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, prominently expressed at intersomitic junctions, in the brain and in several cranial nerves.

Structurei

3D structure databases

ProteinModelPortaliQ91738.
SMRiQ91738. Positions 33-699, 922-1062.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 355321FERM
Add
BLAST
Domaini435 – 693259Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi725 – 74622Pro-rich
Add
BLAST
Compositional biasi876 – 92954Pro-rich
Add
BLAST

Sequence similaritiesi

Contains 1 FERM domain.

Phylogenomic databases

HOVERGENiHBG004018.
KOiK05725.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR005189. Focal_adhesion_kin_target_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF03623. Focal_AT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
ProDomiPD006413. Focal_adhesion_target_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00295. B41. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF68993. SSF68993. 1 hit.
PROSITEiPS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q91738-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAAYLDPNL NHNPSTNAKS RLSTGMERSP GAIERVLRVF HYFESNNEPA     50
TWSSNIRHGD ATDVRGIIQK IVDSHKVKNV ASYGLRLSHL HSEEVHWLHP 100
DIGVSHIREK YEQSHPPEEW KYELRIRYLP KGFVNQFTED KPTLNFFYQQ 150
VKNDYMSEIA DQVDQEIALK LGCLEIRRSY GEMRGNALEK KSNYEVLEKD 200
VGLKRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI LKFFEILSPV 250
YRYDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGSNP THLADFTQVQ 300
TIQYSSSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL 350
VSGASESFII RPQKEGERAL PSIPKLANNE KHGVRPHAVS VSDEFSGDET 400
DDYAEIIDEE DTYTMPSKSY GLDEAGDYEI QRDRIELGRC IGEGQFGDVH 450
QGVYMSPENP AMAVAIKTCK NCTSDSVREK FLQEALTMRQ FDHPHIVKLI 500
GVITENPVWI IMELCTLGEL RSFLQVRKYS LDLASLILYS YQLSTALAYL 550
ESKRFVHRDI AARNVLVSSS DCVKLGDFGL SRYMEDSTYY KASKGKLPIK 600
WMAPESINFR RFTSASDVWM FGVCMWEILM YGVKPFQGVK NNDVIGRIEN 650
GERLPMPPNC PPTLYSLMTK CWAYDPSRRP RFTELKAQLS TILEEEKLQQ 700
EERMRMESRR QVTVSWDSGG SDEAPPKPSR PGYPSPRSSE GFFPSPQHMM 750
QPNHYQVSGF SVAHGIPSMS GNMYPGQASV LDHMDSWNHR TPDINMWQPS 800
MEDSGPMDMR SLAQVLPTHL MEERLIRQQQ EMEEDQRWLE KEERFLKPDV 850
RLSRGSVDHV DGNIQCPAGN QHIYQPVGKP DHVAPPKKPP RPGAPSHLGN 900
LPAHNSPVDG YNEGVKPWRI QPQEISPPPT ANLDRTNDKV YENVTGLVKA 950
VIEMSSRIQP APPEEYVPMV KGVGLALRTL LATVDETIPV LPASTHREIE 1000
MAQKLLNSDL AELINKMKLA QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL 1050
LDVIDQARLK IISHSRPH 1068
Length:1,068
Mass (Da):121,156
Last modified:January 23, 2002 - v2
Checksum:iEF5BEBD60D2C99DA
GO
Isoform Short (identifier: Q91738-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-398: Missing.
     418-425: KSYGLDEA → T
     917-919: Missing.

Show »
Length:1,052
Mass (Da):119,303
Checksum:i05A551229F0BCFF9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei393 – 3986Missing in isoform Short.
VSP_004978
Alternative sequencei418 – 4258KSYGLDEA → T in isoform Short.
VSP_004979
Alternative sequencei917 – 9193Missing in isoform Short.
VSP_004980

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti439 – 4391R → P in AAA99456. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L33920 mRNA. Translation: AAA99456.1.
U11078 mRNA. Translation: AAA80333.1.
PIRiI51670.
JC4200.
RefSeqiNP_001084066.1. NM_001090597.1. [Q91738-1]
UniGeneiXl.6819.

Genome annotation databases

GeneIDi399286.
KEGGixla:399286.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L33920 mRNA. Translation: AAA99456.1 .
U11078 mRNA. Translation: AAA80333.1 .
PIRi I51670.
JC4200.
RefSeqi NP_001084066.1. NM_001090597.1. [Q91738-1 ]
UniGenei Xl.6819.

3D structure databases

ProteinModelPortali Q91738.
SMRi Q91738. Positions 33-699, 922-1062.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 399286.
KEGGi xla:399286.

Organism-specific databases

CTDi 5747.
Xenbasei XB-GENE-952029. ptk2.

Phylogenomic databases

HOVERGENi HBG004018.
KOi K05725.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 6726.

Family and domain databases

Gene3Di 1.20.80.10. 1 hit.
InterProi IPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR005189. Focal_adhesion_kin_target_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF00373. FERM_M. 1 hit.
PF03623. Focal_AT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
PRINTSi PR00109. TYRKINASE.
ProDomi PD006413. Focal_adhesion_target_reg. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00295. B41. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF47031. SSF47031. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF68993. SSF68993. 1 hit.
PROSITEi PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a Xenopus laevis cDNA encoding focal adhesion kinase (FAK) and expression during early development."
    Zhang X., Wright C.V., Hanks S.K.
    Gene 160:219-222(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
  2. "Molecular analysis and developmental expression of the focal adhesion kinase pp125FAK in Xenopus laevis."
    Hens M.D., DeSimone D.W.
    Dev. Biol. 170:274-288(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Embryo.

Entry informationi

Entry nameiFAK1_XENLA
AccessioniPrimary (citable) accession number: Q91738
Secondary accession number(s): Q91563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2002
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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