Reviewed,
UniProtKB/Swiss-Prot Q91738 (FAK1_XENLA)
Last modified
June 16, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Focal adhesion kinase 1 Short name=FADK 1 EC=2.7.10.2 Alternative name(s): pp125FAK | ||||
| Gene names |
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| Organism | Xenopus laevis (African clawed frog) | ||||
| Taxonomic identifier | 8355 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Protein attributes
| Sequence length | 1068 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy By similarity. |
| Catalytic activity | ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. |
| Subcellular location | Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions. |
| Developmental stage | Present in the fertilized egg and in cleavage and blastula stage embryos. During gastrulation, expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, prominently expressed at intersomitic junctions, in the brain and in several cranial nerves. |
| Post-translational modification | Phosphorylated on tyrosine residues; phosphorylated kinase is first detected during gastrulation, suggesting that tyrosine phosphorylation is developmentally regulated. |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell junction Cell membrane Membrane |
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Developmental protein Kinase Transferase Tyrosine-protein kinase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro signal complex assemblyInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoskeleton Inferred from electronic annotation. Source: InterPro focal adhesionInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activityInferred from electronic annotation. Source: EC signal transducer activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: Q91738-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: Q91738-2) The sequence of this isoform differs from the canonical sequence as follows: 393-398: Missing. 418-425: KSYGLDEA → T 917-919: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1068 | 1068 | Focal adhesion kinase 1 | PRO_0000088080 | |||||
Regions | |||||||||
| Domain | 35 – 355 | 321 | FERM | ||||||
| Domain | 435 – 693 | 259 | Protein kinase | ||||||
| Nucleotide binding | 441 – 449 | 9 | ATP By similarity | ||||||
| Compositional bias | 725 – 746 | 22 | Pro-rich | ||||||
| Compositional bias | 876 – 929 | 54 | Pro-rich | ||||||
Sites | |||||||||
| Active site | 559 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 467 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 403 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 413 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 589 | 1 | Phosphotyrosine; by autocatalysis By similarity | ||||||
| Modified residue | 590 | 1 | Phosphotyrosine; by autocatalysis By similarity | ||||||
| Modified residue | 874 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 941 | 1 | Phosphotyrosine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 393 – 398 | 6 | Missing in isoform Short. | VSP_004978 | |||||
| Alternative sequence | 418 – 425 | 8 | KSYGLDEA → T in isoform Short. | VSP_004979 | |||||
| Alternative sequence | 917 – 919 | 3 | Missing in isoform Short. | VSP_004980 | |||||
Experimental info | |||||||||
| Sequence conflict | 439 | 1 | R → P in AAA99456. Ref.2 | ||||||
Sequences
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References
| [1] | "Cloning of a Xenopus laevis cDNA encoding focal adhesion kinase (FAK) and expression during early development." Zhang X., Wright C.V., Hanks S.K. Gene 160:219-222(1995) [PubMed: 7642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). |
| [2] | "Molecular analysis and developmental expression of the focal adhesion kinase pp125FAK in Xenopus laevis." Hens M.D., DeSimone D.W. Dev. Biol. 170:274-288(1995) [PubMed: 7649362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). Tissue: Embryo. |
Cross-references
Sequence databases | |
|---|---|
| L33920 mRNA. Translation: AAA99456.1. U11078 mRNA. Translation: AAA80333.1. | |
| PIR | I51670. JC4200. |
| RefSeq | NP_001084066.1. |
| UniGene | Xl.6819 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1K05 based on UniProtKB Q05397. |
| SMR | Q91738. Positions 33-383, 428-699, 924-1065. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 399286. |
| KEGG | xla:399286. |
Organism-specific databases | |
| Xenbase | XB-FEAT-952024. ptk2. |
Phylogenomic databases | |
| HOVERGEN | Q91738. |
Enzyme and pathway databases | |
| BRENDA | 2.7.10.2. 648. |
Family and domain databases | |
| InterPro | IPR019749. Band_41_domain. IPR019748. FERM_central. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR005189. Focal_adhesion_target_reg. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view] |
| Pfam | PF00373. FERM_M. 1 hit. PF03623. Focal_AT. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view] |
| PRINTS | PR00109. TYRKINASE. |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00295. B41. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view] |
| PROSITE | PS00660. FERM_1. False negative. PS00661. FERM_2. 1 hit. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FAK1_XENLA | ||||||||
| Accession | Primary (citable) accession number: Q91738 Secondary accession number(s): Q91563 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Xenopus annotation project | ||||||||
