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Q91738

- FAK1_XENLA

UniProt

Q91738 - FAK1_XENLA

Protein

Focal adhesion kinase 1

Gene

ptk2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei467 – 4671ATPPROSITE-ProRule annotation
    Active sitei559 – 5591Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi441 – 4477ATPPROSITE-ProRule annotation
    Nucleotide bindingi513 – 5153ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. signal transducer activity Source: InterPro

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. signal complex assembly Source: InterPro

    Keywords - Molecular functioni

    Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 6726.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Focal adhesion kinase 1 (EC:2.7.10.2)
    Short name:
    FADK 1
    Alternative name(s):
    Protein-tyrosine kinase 2
    pp125FAK
    Gene namesi
    Name:ptk2
    Synonyms:fak1
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-952029. ptk2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoskeleton Source: InterPro
    2. focal adhesion Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10681068Focal adhesion kinase 1PRO_0000088080Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei403 – 4031PhosphotyrosineBy similarity
    Modified residuei413 – 4131PhosphotyrosineBy similarity
    Modified residuei589 – 5891Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei590 – 5901Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei874 – 8741PhosphotyrosineBy similarity
    Modified residuei941 – 9411PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine residues; phosphorylated kinase is first detected during gastrulation, suggesting that tyrosine phosphorylation is developmentally regulated.

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Present in the fertilized egg and in cleavage and blastula stage embryos. During gastrulation, expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, prominently expressed at intersomitic junctions, in the brain and in several cranial nerves.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91738.
    SMRiQ91738. Positions 33-699, 922-1062.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 355321FERMPROSITE-ProRule annotationAdd
    BLAST
    Domaini435 – 693259Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi725 – 74622Pro-richAdd
    BLAST
    Compositional biasi876 – 92954Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.PROSITE-ProRule annotation
    Contains 1 FERM domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG004018.
    KOiK05725.

    Family and domain databases

    Gene3Di1.20.80.10. 1 hit.
    InterProiIPR019749. Band_41_domain.
    IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
    IPR019748. FERM_central.
    IPR000299. FERM_domain.
    IPR005189. Focal_adhesion_kin_target_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00373. FERM_M. 1 hit.
    PF03623. Focal_AT. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    ProDomiPD006413. Focal_adhesion_target_reg. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00295. B41. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47031. SSF47031. 1 hit.
    SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    SSF68993. SSF68993. 1 hit.
    PROSITEiPS00661. FERM_2. 1 hit.
    PS50057. FERM_3. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q91738-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAAYLDPNL NHNPSTNAKS RLSTGMERSP GAIERVLRVF HYFESNNEPA     50
    TWSSNIRHGD ATDVRGIIQK IVDSHKVKNV ASYGLRLSHL HSEEVHWLHP 100
    DIGVSHIREK YEQSHPPEEW KYELRIRYLP KGFVNQFTED KPTLNFFYQQ 150
    VKNDYMSEIA DQVDQEIALK LGCLEIRRSY GEMRGNALEK KSNYEVLEKD 200
    VGLKRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI LKFFEILSPV 250
    YRYDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGSNP THLADFTQVQ 300
    TIQYSSSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL 350
    VSGASESFII RPQKEGERAL PSIPKLANNE KHGVRPHAVS VSDEFSGDET 400
    DDYAEIIDEE DTYTMPSKSY GLDEAGDYEI QRDRIELGRC IGEGQFGDVH 450
    QGVYMSPENP AMAVAIKTCK NCTSDSVREK FLQEALTMRQ FDHPHIVKLI 500
    GVITENPVWI IMELCTLGEL RSFLQVRKYS LDLASLILYS YQLSTALAYL 550
    ESKRFVHRDI AARNVLVSSS DCVKLGDFGL SRYMEDSTYY KASKGKLPIK 600
    WMAPESINFR RFTSASDVWM FGVCMWEILM YGVKPFQGVK NNDVIGRIEN 650
    GERLPMPPNC PPTLYSLMTK CWAYDPSRRP RFTELKAQLS TILEEEKLQQ 700
    EERMRMESRR QVTVSWDSGG SDEAPPKPSR PGYPSPRSSE GFFPSPQHMM 750
    QPNHYQVSGF SVAHGIPSMS GNMYPGQASV LDHMDSWNHR TPDINMWQPS 800
    MEDSGPMDMR SLAQVLPTHL MEERLIRQQQ EMEEDQRWLE KEERFLKPDV 850
    RLSRGSVDHV DGNIQCPAGN QHIYQPVGKP DHVAPPKKPP RPGAPSHLGN 900
    LPAHNSPVDG YNEGVKPWRI QPQEISPPPT ANLDRTNDKV YENVTGLVKA 950
    VIEMSSRIQP APPEEYVPMV KGVGLALRTL LATVDETIPV LPASTHREIE 1000
    MAQKLLNSDL AELINKMKLA QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL 1050
    LDVIDQARLK IISHSRPH 1068
    Length:1,068
    Mass (Da):121,156
    Last modified:January 23, 2002 - v2
    Checksum:iEF5BEBD60D2C99DA
    GO
    Isoform Short (identifier: Q91738-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         393-398: Missing.
         418-425: KSYGLDEA → T
         917-919: Missing.

    Show »
    Length:1,052
    Mass (Da):119,303
    Checksum:i05A551229F0BCFF9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti439 – 4391R → P in AAA99456. (PubMed:7649362)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei393 – 3986Missing in isoform Short. 1 PublicationVSP_004978
    Alternative sequencei418 – 4258KSYGLDEA → T in isoform Short. 1 PublicationVSP_004979
    Alternative sequencei917 – 9193Missing in isoform Short. 1 PublicationVSP_004980

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33920 mRNA. Translation: AAA99456.1.
    U11078 mRNA. Translation: AAA80333.1.
    PIRiI51670.
    JC4200.
    RefSeqiNP_001084066.1. NM_001090597.1. [Q91738-1]
    UniGeneiXl.6819.

    Genome annotation databases

    GeneIDi399286.
    KEGGixla:399286.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33920 mRNA. Translation: AAA99456.1 .
    U11078 mRNA. Translation: AAA80333.1 .
    PIRi I51670.
    JC4200.
    RefSeqi NP_001084066.1. NM_001090597.1. [Q91738-1 ]
    UniGenei Xl.6819.

    3D structure databases

    ProteinModelPortali Q91738.
    SMRi Q91738. Positions 33-699, 922-1062.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399286.
    KEGGi xla:399286.

    Organism-specific databases

    CTDi 5747.
    Xenbasei XB-GENE-952029. ptk2.

    Phylogenomic databases

    HOVERGENi HBG004018.
    KOi K05725.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 6726.

    Family and domain databases

    Gene3Di 1.20.80.10. 1 hit.
    InterProi IPR019749. Band_41_domain.
    IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
    IPR019748. FERM_central.
    IPR000299. FERM_domain.
    IPR005189. Focal_adhesion_kin_target_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00373. FERM_M. 1 hit.
    PF03623. Focal_AT. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    ProDomi PD006413. Focal_adhesion_target_reg. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00295. B41. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47031. SSF47031. 1 hit.
    SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    SSF68993. SSF68993. 1 hit.
    PROSITEi PS00661. FERM_2. 1 hit.
    PS50057. FERM_3. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a Xenopus laevis cDNA encoding focal adhesion kinase (FAK) and expression during early development."
      Zhang X., Wright C.V., Hanks S.K.
      Gene 160:219-222(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    2. "Molecular analysis and developmental expression of the focal adhesion kinase pp125FAK in Xenopus laevis."
      Hens M.D., DeSimone D.W.
      Dev. Biol. 170:274-288(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
      Tissue: Embryo.

    Entry informationi

    Entry nameiFAK1_XENLA
    AccessioniPrimary (citable) accession number: Q91738
    Secondary accession number(s): Q91563
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3