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Protein

Focal adhesion kinase 1

Gene

ptk2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei467ATPPROSITE-ProRule annotation1
Active sitei559Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi441 – 447ATPPROSITE-ProRule annotation7
Nucleotide bindingi513 – 515ATPPROSITE-ProRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 6725

Names & Taxonomyi

Protein namesi
Recommended name:
Focal adhesion kinase 1 (EC:2.7.10.2)
Short name:
FADK 1
Alternative name(s):
Protein-tyrosine kinase 2
pp125FAK
Gene namesi
Name:ptk2
Synonyms:fak1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-952029 ptk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880801 – 1068Focal adhesion kinase 1Add BLAST1068

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei403PhosphotyrosineBy similarity1
Modified residuei413PhosphotyrosineBy similarity1
Modified residuei589Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei590Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei874PhosphotyrosineBy similarity1
Modified residuei941PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues; phosphorylated kinase is first detected during gastrulation, suggesting that tyrosine phosphorylation is developmentally regulated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ91738

Expressioni

Developmental stagei

Present in the fertilized egg and in cleavage and blastula stage embryos. During gastrulation, expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, prominently expressed at intersomitic junctions, in the brain and in several cranial nerves.

Structurei

3D structure databases

ProteinModelPortaliQ91738
SMRiQ91738
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 355FERMPROSITE-ProRule annotationAdd BLAST321
Domaini435 – 693Protein kinasePROSITE-ProRule annotationAdd BLAST259

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi725 – 746Pro-richAdd BLAST22
Compositional biasi876 – 929Pro-richAdd BLAST54

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG004018
KOiK05725

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR000299 FERM_domain
IPR036137 Focal_adhe_kin_target_dom_sf
IPR005189 Focal_adhesion_kin_target_dom
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00373 FERM_M, 1 hit
PF03623 Focal_AT, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006413 Focal_adhesion_target_reg, 1 hit
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
SSF68993 SSF68993, 1 hit
PROSITEiView protein in PROSITE
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q91738-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAYLDPNL NHNPSTNAKS RLSTGMERSP GAIERVLRVF HYFESNNEPA
60 70 80 90 100
TWSSNIRHGD ATDVRGIIQK IVDSHKVKNV ASYGLRLSHL HSEEVHWLHP
110 120 130 140 150
DIGVSHIREK YEQSHPPEEW KYELRIRYLP KGFVNQFTED KPTLNFFYQQ
160 170 180 190 200
VKNDYMSEIA DQVDQEIALK LGCLEIRRSY GEMRGNALEK KSNYEVLEKD
210 220 230 240 250
VGLKRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI LKFFEILSPV
260 270 280 290 300
YRYDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGSNP THLADFTQVQ
310 320 330 340 350
TIQYSSSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL
360 370 380 390 400
VSGASESFII RPQKEGERAL PSIPKLANNE KHGVRPHAVS VSDEFSGDET
410 420 430 440 450
DDYAEIIDEE DTYTMPSKSY GLDEAGDYEI QRDRIELGRC IGEGQFGDVH
460 470 480 490 500
QGVYMSPENP AMAVAIKTCK NCTSDSVREK FLQEALTMRQ FDHPHIVKLI
510 520 530 540 550
GVITENPVWI IMELCTLGEL RSFLQVRKYS LDLASLILYS YQLSTALAYL
560 570 580 590 600
ESKRFVHRDI AARNVLVSSS DCVKLGDFGL SRYMEDSTYY KASKGKLPIK
610 620 630 640 650
WMAPESINFR RFTSASDVWM FGVCMWEILM YGVKPFQGVK NNDVIGRIEN
660 670 680 690 700
GERLPMPPNC PPTLYSLMTK CWAYDPSRRP RFTELKAQLS TILEEEKLQQ
710 720 730 740 750
EERMRMESRR QVTVSWDSGG SDEAPPKPSR PGYPSPRSSE GFFPSPQHMM
760 770 780 790 800
QPNHYQVSGF SVAHGIPSMS GNMYPGQASV LDHMDSWNHR TPDINMWQPS
810 820 830 840 850
MEDSGPMDMR SLAQVLPTHL MEERLIRQQQ EMEEDQRWLE KEERFLKPDV
860 870 880 890 900
RLSRGSVDHV DGNIQCPAGN QHIYQPVGKP DHVAPPKKPP RPGAPSHLGN
910 920 930 940 950
LPAHNSPVDG YNEGVKPWRI QPQEISPPPT ANLDRTNDKV YENVTGLVKA
960 970 980 990 1000
VIEMSSRIQP APPEEYVPMV KGVGLALRTL LATVDETIPV LPASTHREIE
1010 1020 1030 1040 1050
MAQKLLNSDL AELINKMKLA QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL
1060
LDVIDQARLK IISHSRPH
Length:1,068
Mass (Da):121,156
Last modified:January 23, 2002 - v2
Checksum:iEF5BEBD60D2C99DA
GO
Isoform Short (identifier: Q91738-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-398: Missing.
     418-425: KSYGLDEA → T
     917-919: Missing.

Show »
Length:1,052
Mass (Da):119,303
Checksum:i05A551229F0BCFF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti439R → P in AAA99456 (PubMed:7649362).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004978393 – 398Missing in isoform Short. 1 Publication6
Alternative sequenceiVSP_004979418 – 425KSYGLDEA → T in isoform Short. 1 Publication8
Alternative sequenceiVSP_004980917 – 919Missing in isoform Short. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33920 mRNA Translation: AAA99456.1
U11078 mRNA Translation: AAA80333.1
PIRiI51670
JC4200
RefSeqiNP_001084066.1, NM_001090597.1 [Q91738-1]
UniGeneiXl.6819

Genome annotation databases

GeneIDi399286
KEGGixla:399286

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFAK1_XENLA
AccessioniPrimary (citable) accession number: Q91738
Secondary accession number(s): Q91563
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2002
Last modified: May 23, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health