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Q91738 (FAK1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Focal adhesion kinase 1
Short name=FADK 1
EC=2.7.10.2
Alternative name(s):
Protein-tyrosine kinase 2
pp125FAK
Gene names
Name:ptk2
Synonyms:fak1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length1068 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions.

Developmental stage

Present in the fertilized egg and in cleavage and blastula stage embryos. During gastrulation, expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, prominently expressed at intersomitic junctions, in the brain and in several cranial nerves.

Post-translational modification

Phosphorylated on tyrosine residues; phosphorylated kinase is first detected during gastrulation, suggesting that tyrosine phosphorylation is developmentally regulated.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Contains 1 FERM domain.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q91738-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q91738-2)

The sequence of this isoform differs from the canonical sequence as follows:
     393-398: Missing.
     418-425: KSYGLDEA → T
     917-919: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10681068Focal adhesion kinase 1
PRO_0000088080

Regions

Domain35 – 355321FERM
Domain435 – 693259Protein kinase
Nucleotide binding441 – 4477ATP By similarity
Nucleotide binding513 – 5153ATP By similarity
Compositional bias725 – 74622Pro-rich
Compositional bias876 – 92954Pro-rich

Sites

Active site5591Proton acceptor By similarity
Binding site4671ATP By similarity

Amino acid modifications

Modified residue4031Phosphotyrosine By similarity
Modified residue4131Phosphotyrosine By similarity
Modified residue5891Phosphotyrosine; by autocatalysis By similarity
Modified residue5901Phosphotyrosine; by autocatalysis By similarity
Modified residue8741Phosphotyrosine By similarity
Modified residue9411Phosphotyrosine By similarity

Natural variations

Alternative sequence393 – 3986Missing in isoform Short.
VSP_004978
Alternative sequence418 – 4258KSYGLDEA → T in isoform Short.
VSP_004979
Alternative sequence917 – 9193Missing in isoform Short.
VSP_004980

Experimental info

Sequence conflict4391R → P in AAA99456. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: EF5BEBD60D2C99DA

FASTA1,068121,156
        10         20         30         40         50         60 
MAAAYLDPNL NHNPSTNAKS RLSTGMERSP GAIERVLRVF HYFESNNEPA TWSSNIRHGD 

        70         80         90        100        110        120 
ATDVRGIIQK IVDSHKVKNV ASYGLRLSHL HSEEVHWLHP DIGVSHIREK YEQSHPPEEW 

       130        140        150        160        170        180 
KYELRIRYLP KGFVNQFTED KPTLNFFYQQ VKNDYMSEIA DQVDQEIALK LGCLEIRRSY 

       190        200        210        220        230        240 
GEMRGNALEK KSNYEVLEKD VGLKRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI 

       250        260        270        280        290        300 
LKFFEILSPV YRYDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGSNP THLADFTQVQ 

       310        320        330        340        350        360 
TIQYSSSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL VSGASESFII 

       370        380        390        400        410        420 
RPQKEGERAL PSIPKLANNE KHGVRPHAVS VSDEFSGDET DDYAEIIDEE DTYTMPSKSY 

       430        440        450        460        470        480 
GLDEAGDYEI QRDRIELGRC IGEGQFGDVH QGVYMSPENP AMAVAIKTCK NCTSDSVREK 

       490        500        510        520        530        540 
FLQEALTMRQ FDHPHIVKLI GVITENPVWI IMELCTLGEL RSFLQVRKYS LDLASLILYS 

       550        560        570        580        590        600 
YQLSTALAYL ESKRFVHRDI AARNVLVSSS DCVKLGDFGL SRYMEDSTYY KASKGKLPIK 

       610        620        630        640        650        660 
WMAPESINFR RFTSASDVWM FGVCMWEILM YGVKPFQGVK NNDVIGRIEN GERLPMPPNC 

       670        680        690        700        710        720 
PPTLYSLMTK CWAYDPSRRP RFTELKAQLS TILEEEKLQQ EERMRMESRR QVTVSWDSGG 

       730        740        750        760        770        780 
SDEAPPKPSR PGYPSPRSSE GFFPSPQHMM QPNHYQVSGF SVAHGIPSMS GNMYPGQASV 

       790        800        810        820        830        840 
LDHMDSWNHR TPDINMWQPS MEDSGPMDMR SLAQVLPTHL MEERLIRQQQ EMEEDQRWLE 

       850        860        870        880        890        900 
KEERFLKPDV RLSRGSVDHV DGNIQCPAGN QHIYQPVGKP DHVAPPKKPP RPGAPSHLGN 

       910        920        930        940        950        960 
LPAHNSPVDG YNEGVKPWRI QPQEISPPPT ANLDRTNDKV YENVTGLVKA VIEMSSRIQP 

       970        980        990       1000       1010       1020 
APPEEYVPMV KGVGLALRTL LATVDETIPV LPASTHREIE MAQKLLNSDL AELINKMKLA 

      1030       1040       1050       1060 
QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL LDVIDQARLK IISHSRPH

« Hide

Isoform Short [UniParc].

Checksum: 05A551229F0BCFF9
Show »

FASTA1,052119,303

References

[1]"Cloning of a Xenopus laevis cDNA encoding focal adhesion kinase (FAK) and expression during early development."
Zhang X., Wright C.V., Hanks S.K.
Gene 160:219-222(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]"Molecular analysis and developmental expression of the focal adhesion kinase pp125FAK in Xenopus laevis."
Hens M.D., DeSimone D.W.
Dev. Biol. 170:274-288(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L33920 mRNA. Translation: AAA99456.1.
U11078 mRNA. Translation: AAA80333.1.
PIRI51670.
JC4200.
RefSeqNP_001084066.1. NM_001090597.1.
UniGeneXl.6819.

3D structure databases

ProteinModelPortalQ91738.
SMRQ91738. Positions 33-699, 922-1062.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399286.
KEGGxla:399286.

Organism-specific databases

CTD5747.
XenbaseXB-GENE-952029. ptk2.

Phylogenomic databases

HOVERGENHBG004018.
KOK05725.

Enzyme and pathway databases

BRENDA2.7.10.2. 6726.

Family and domain databases

Gene3D1.20.80.10. 1 hit.
InterProIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR005189. Focal_adhesion_kin_target_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF00373. FERM_M. 1 hit.
PF03623. Focal_AT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
ProDomPD006413. Focal_adhesion_target_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00295. B41. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF47031. FERM_3-hlx. 1 hit.
SSF68993. Focal_AT. 1 hit.
SSF56112. Kinase_like. 1 hit.
PROSITEPS00660. FERM_1. False negative.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFAK1_XENLA
AccessionPrimary (citable) accession number: Q91738
Secondary accession number(s): Q91563
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2002
Last modified: April 3, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents