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Q91713

- CHRD_XENLA

UniProt

Q91713 - CHRD_XENLA

Protein

Chordin

Gene

chrd

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Potent dorsalizing factor. Has potent axis-forming activities including the ability to recruit neighboring cells into secondary axes. Regulates cell-cell interactions in the organizing centers of head, trunk and tail development.1 Publication

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. syndecan binding Source: UniProtKB

    GO - Biological processi

    1. axis specification Source: UniProtKB
    2. chordate embryonic development Source: UniProtKB
    3. post-anal tail morphogenesis Source: UniProtKB
    4. pronephros development Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chordin
    Alternative name(s):
    Organizer-specific secreted-dorsalizing factor
    Gene namesi
    Name:chrd
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-865395. chrd.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 941922ChordinPRO_0000005367Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Cleaved by bmp1; cleavage participates in dorsoventral patterning during early development. Cleavage by bmp1 is enhanced by the interaction with olfml3/ont1.1 Publication

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    First expressed at stage 9.5 in the dorsal marginal zone. Localized to the dorsal lip of the blastopore (Spemann organizer) during early gastrulation, after which expression continues in tissues derived from the organizer. Expressed in the prechordal plate (head mesoderm) and notochord during early neurulation (stage 13), transiently in the forebrain, tailbud hinge and posterior notochord in the early tailbud (stage 26), the chordoneural hinge in the late tailbud (stage 33) and finally the tip of the tail in the tadpole (stage 42).1 Publication

    Inductioni

    By activin in the presence of de novo protein synthesis, and by gsc and not2.2 Publications

    Interactioni

    Subunit structurei

    Interacts with twsg1 and/or bmp4. Interacts with olfml3/ont1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    olfml3B5MFE96EBI-1997746,EBI-1997734
    tll1Q8JI282EBI-1997746,EBI-1997794

    Protein-protein interaction databases

    IntActiQ91713. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91713.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 11777VWFC 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini161 – 279119CHRD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini281 – 401121CHRD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini402 – 522121CHRD 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini528 – 655128CHRD 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini691 – 75161VWFC 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini769 – 83870VWFC 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini857 – 91963VWFC 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the chordin family.Curated
    Contains 4 CHRD domains.PROSITE-ProRule annotation
    Contains 4 VWFC domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOVERGENiHBG005330.
    KOiK04657.

    Family and domain databases

    InterProiIPR016353. Chordin.
    IPR010895. CHRD.
    IPR001007. VWF_C.
    [Graphical view]
    PfamiPF07452. CHRD. 4 hits.
    PF00093. VWC. 3 hits.
    [Graphical view]
    PIRSFiPIRSF002496. Chordin. 1 hit.
    SMARTiSM00754. CHRD. 4 hits.
    SM00214. VWC. 4 hits.
    [Graphical view]
    PROSITEiPS50933. CHRD. 4 hits.
    PS01208. VWFC_1. 2 hits.
    PS50184. VWFC_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91713-1 [UniParc]FASTAAdd to Basket

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    MQCPPILLVW TLWIMAVDCS RPKVFLPIQP EQEPLQSKTP AGCTFGGKFY    50
    SLEDSWHPDL GEPFGVMHCV LCYCEPQRSR RGKPSGKVSC KNIKHDCPSP 100
    SCANPILLPL HCCKTCPKAP PPPIKKSDFV FDGFEYFQEK DDDLYNDRSY 150
    LSSDDVAVEE SRSEYVALLT APSHVWPPVT SGVAKARFNL QRSNLLFSIT 200
    YKWIDRLSRI RFSDLDGSVL FEHPVHRMGS PRDDTICGIW RSLNRSTLRL 250
    LRMGHILVSL VTTTLSEPEI SGKIVKHKAL FSESFSALLT PEDSDETGGG 300
    GLAMLTLSDV DDNLHFILML RGLSGEEGDQ IPILVQISHQ NHVIRELYAN 350
    ISAQEQDFAE VLPDLSSREM LWLAQGQLEI SVQTEGRRPQ SMSGIITVRK 400
    SCDTLQSVLS GGDALNPTKT GAVGSASITL HENGTLEYQI QIAGTMSTVT 450
    AVTLETKPRR KTKRNILHDM SKDYHDGRVW GYWIDANARD LHMLLQSELF 500
    LNVATKDFQE GELRGQITPL LYSGLWARYE KLPVPLAGQF VSPPIRTGSA 550
    GHAWVSLDEH CHLHYQIVVT GLGKAEDAAL NAHLHGFAEL GEVGESSPGH 600
    KRLLKGFYGS EAQGSVKDLD LELLGHLSRG TAFIQVSTKL NPRGEIRGQI 650
    HIPNSCESGG VSLTPEEPEY EYEIYEEGRQ RDPDDLRKDP RACSFEGQLR 700
    AHGSRWAPDY DRKCSVCSCQ KRTVICDPIV CPPLNCSQPV HLPDQCCPVC 750
    EEKKEMREVK KPERARTSEG CFFDGDRSWK AAGTRWHPFV PPFGLIKCAI 800
    CTCKGSTGEV HCEKVTCPKL SCTNPIRANP SDCCKQCPVE ERSPMELADS 850
    MQSDGAGSCR FGRHWYPNHE RWHPTVPPFG EMKCVTCTCA EGITQCRRQE 900
    CTGTTCGTGS KRDRCCTKCK DANQDEDEKV KSDETRTPWS F 941
    Length:941
    Mass (Da):104,947
    Last modified:November 1, 1996 - v1
    Checksum:i7D1C3262064C7323
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1101L → G in AAH77767. 1 PublicationCurated
    Sequence conflicti839 – 8391Missing in AAH77767. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35764 mRNA. Translation: AAC42222.1.
    BC077767 mRNA. Translation: AAH77767.1.
    PIRiA55195.
    RefSeqiNP_001081778.1. NM_001088309.1.
    UniGeneiXl.3549.

    Genome annotation databases

    GeneIDi398045.
    KEGGixla:398045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35764 mRNA. Translation: AAC42222.1 .
    BC077767 mRNA. Translation: AAH77767.1 .
    PIRi A55195.
    RefSeqi NP_001081778.1. NM_001088309.1.
    UniGenei Xl.3549.

    3D structure databases

    ProteinModelPortali Q91713.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q91713. 4 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398045.
    KEGGi xla:398045.

    Organism-specific databases

    CTDi 8646.
    Xenbasei XB-GENE-865395. chrd.

    Phylogenomic databases

    HOVERGENi HBG005330.
    KOi K04657.

    Family and domain databases

    InterProi IPR016353. Chordin.
    IPR010895. CHRD.
    IPR001007. VWF_C.
    [Graphical view ]
    Pfami PF07452. CHRD. 4 hits.
    PF00093. VWC. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF002496. Chordin. 1 hit.
    SMARTi SM00754. CHRD. 4 hits.
    SM00214. VWC. 4 hits.
    [Graphical view ]
    PROSITEi PS50933. CHRD. 4 hits.
    PS01208. VWFC_1. 2 hits.
    PS50184. VWFC_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes."
      Sasai Y., Lu B., Steinbeisser H., Geissert D., Gont L.K., De Robertis E.M.
      Cell 79:779-790(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Tissue: Dorsal lip.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.
    3. "Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus."
      Gont L.K., Fainsod A., Kim S.-H., De Robertis E.M.
      Dev. Biol. 174:174-178(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    4. "Is chordin a long-range- or short-range-acting factor? Roles for BMP1-related metalloproteases in chordin and BMP4 autofeedback loop regulation."
      Blitz I.L., Shimmi O., Wuennenberg-Stapleton K., O'Connor M.B., Cho K.W.Y.
      Dev. Biol. 223:120-138(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE.
    5. "The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling."
      Oelgeschlager M., Larrain J., Geissert D., De Robertis E.M.
      Nature 405:757-763(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TWSG1 AND BMP4.
    6. "Robust stability of the embryonic axial pattern requires a secreted scaffold for chordin degradation."
      Inomata H., Haraguchi T., Sasai Y.
      Cell 134:854-865(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OLFML3.

    Entry informationi

    Entry nameiCHRD_XENLA
    AccessioniPrimary (citable) accession number: Q91713
    Secondary accession number(s): Q6DD60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3