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Q91713

- CHRD_XENLA

UniProt

Q91713 - CHRD_XENLA

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Protein

Chordin

Gene

chrd

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Potent dorsalizing factor. Has potent axis-forming activities including the ability to recruit neighboring cells into secondary axes. Regulates cell-cell interactions in the organizing centers of head, trunk and tail development.1 Publication

GO - Molecular functioni

  1. heparin binding Source: UniProtKB
  2. syndecan binding Source: UniProtKB

GO - Biological processi

  1. axis specification Source: UniProtKB
  2. chordate embryonic development Source: UniProtKB
  3. post-anal tail morphogenesis Source: UniProtKB
  4. pronephros development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Chordin
Alternative name(s):
Organizer-specific secreted-dorsalizing factor
Gene namesi
Name:chrd
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865395. chrd.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 941922ChordinPRO_0000005367Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Cleaved by bmp1; cleavage participates in dorsoventral patterning during early development. Cleavage by bmp1 is enhanced by the interaction with olfml3/ont1.1 Publication

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

First expressed at stage 9.5 in the dorsal marginal zone. Localized to the dorsal lip of the blastopore (Spemann organizer) during early gastrulation, after which expression continues in tissues derived from the organizer. Expressed in the prechordal plate (head mesoderm) and notochord during early neurulation (stage 13), transiently in the forebrain, tailbud hinge and posterior notochord in the early tailbud (stage 26), the chordoneural hinge in the late tailbud (stage 33) and finally the tip of the tail in the tadpole (stage 42).1 Publication

Inductioni

By activin in the presence of de novo protein synthesis, and by gsc and not2.2 Publications

Interactioni

Subunit structurei

Interacts with twsg1 and/or bmp4. Interacts with olfml3/ont1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
olfml3B5MFE96EBI-1997746,EBI-1997734
tll1Q8JI282EBI-1997746,EBI-1997794

Protein-protein interaction databases

IntActiQ91713. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ91713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 11777VWFC 1PROSITE-ProRule annotationAdd
BLAST
Domaini161 – 279119CHRD 1PROSITE-ProRule annotationAdd
BLAST
Domaini281 – 401121CHRD 2PROSITE-ProRule annotationAdd
BLAST
Domaini402 – 522121CHRD 3PROSITE-ProRule annotationAdd
BLAST
Domaini528 – 655128CHRD 4PROSITE-ProRule annotationAdd
BLAST
Domaini691 – 75161VWFC 2PROSITE-ProRule annotationAdd
BLAST
Domaini769 – 83870VWFC 3PROSITE-ProRule annotationAdd
BLAST
Domaini857 – 91963VWFC 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the chordin family.Curated
Contains 4 CHRD domains.PROSITE-ProRule annotation
Contains 4 VWFC domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG005330.
KOiK04657.

Family and domain databases

InterProiIPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_C.
[Graphical view]
PfamiPF07452. CHRD. 4 hits.
PF00093. VWC. 3 hits.
[Graphical view]
PIRSFiPIRSF002496. Chordin. 1 hit.
SMARTiSM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view]
PROSITEiPS50933. CHRD. 4 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91713-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQCPPILLVW TLWIMAVDCS RPKVFLPIQP EQEPLQSKTP AGCTFGGKFY
60 70 80 90 100
SLEDSWHPDL GEPFGVMHCV LCYCEPQRSR RGKPSGKVSC KNIKHDCPSP
110 120 130 140 150
SCANPILLPL HCCKTCPKAP PPPIKKSDFV FDGFEYFQEK DDDLYNDRSY
160 170 180 190 200
LSSDDVAVEE SRSEYVALLT APSHVWPPVT SGVAKARFNL QRSNLLFSIT
210 220 230 240 250
YKWIDRLSRI RFSDLDGSVL FEHPVHRMGS PRDDTICGIW RSLNRSTLRL
260 270 280 290 300
LRMGHILVSL VTTTLSEPEI SGKIVKHKAL FSESFSALLT PEDSDETGGG
310 320 330 340 350
GLAMLTLSDV DDNLHFILML RGLSGEEGDQ IPILVQISHQ NHVIRELYAN
360 370 380 390 400
ISAQEQDFAE VLPDLSSREM LWLAQGQLEI SVQTEGRRPQ SMSGIITVRK
410 420 430 440 450
SCDTLQSVLS GGDALNPTKT GAVGSASITL HENGTLEYQI QIAGTMSTVT
460 470 480 490 500
AVTLETKPRR KTKRNILHDM SKDYHDGRVW GYWIDANARD LHMLLQSELF
510 520 530 540 550
LNVATKDFQE GELRGQITPL LYSGLWARYE KLPVPLAGQF VSPPIRTGSA
560 570 580 590 600
GHAWVSLDEH CHLHYQIVVT GLGKAEDAAL NAHLHGFAEL GEVGESSPGH
610 620 630 640 650
KRLLKGFYGS EAQGSVKDLD LELLGHLSRG TAFIQVSTKL NPRGEIRGQI
660 670 680 690 700
HIPNSCESGG VSLTPEEPEY EYEIYEEGRQ RDPDDLRKDP RACSFEGQLR
710 720 730 740 750
AHGSRWAPDY DRKCSVCSCQ KRTVICDPIV CPPLNCSQPV HLPDQCCPVC
760 770 780 790 800
EEKKEMREVK KPERARTSEG CFFDGDRSWK AAGTRWHPFV PPFGLIKCAI
810 820 830 840 850
CTCKGSTGEV HCEKVTCPKL SCTNPIRANP SDCCKQCPVE ERSPMELADS
860 870 880 890 900
MQSDGAGSCR FGRHWYPNHE RWHPTVPPFG EMKCVTCTCA EGITQCRRQE
910 920 930 940
CTGTTCGTGS KRDRCCTKCK DANQDEDEKV KSDETRTPWS F
Length:941
Mass (Da):104,947
Last modified:November 1, 1996 - v1
Checksum:i7D1C3262064C7323
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101L → G in AAH77767. 1 PublicationCurated
Sequence conflicti839 – 8391Missing in AAH77767. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35764 mRNA. Translation: AAC42222.1.
BC077767 mRNA. Translation: AAH77767.1.
PIRiA55195.
RefSeqiNP_001081778.1. NM_001088309.1.
UniGeneiXl.3549.

Genome annotation databases

GeneIDi398045.
KEGGixla:398045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35764 mRNA. Translation: AAC42222.1 .
BC077767 mRNA. Translation: AAH77767.1 .
PIRi A55195.
RefSeqi NP_001081778.1. NM_001088309.1.
UniGenei Xl.3549.

3D structure databases

ProteinModelPortali Q91713.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91713. 4 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398045.
KEGGi xla:398045.

Organism-specific databases

CTDi 8646.
Xenbasei XB-GENE-865395. chrd.

Phylogenomic databases

HOVERGENi HBG005330.
KOi K04657.

Family and domain databases

InterProi IPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_C.
[Graphical view ]
Pfami PF07452. CHRD. 4 hits.
PF00093. VWC. 3 hits.
[Graphical view ]
PIRSFi PIRSF002496. Chordin. 1 hit.
SMARTi SM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view ]
PROSITEi PS50933. CHRD. 4 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes."
    Sasai Y., Lu B., Steinbeisser H., Geissert D., Gont L.K., De Robertis E.M.
    Cell 79:779-790(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Tissue: Dorsal lip.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  3. "Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus."
    Gont L.K., Fainsod A., Kim S.-H., De Robertis E.M.
    Dev. Biol. 174:174-178(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. "Is chordin a long-range- or short-range-acting factor? Roles for BMP1-related metalloproteases in chordin and BMP4 autofeedback loop regulation."
    Blitz I.L., Shimmi O., Wuennenberg-Stapleton K., O'Connor M.B., Cho K.W.Y.
    Dev. Biol. 223:120-138(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE.
  5. "The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling."
    Oelgeschlager M., Larrain J., Geissert D., De Robertis E.M.
    Nature 405:757-763(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TWSG1 AND BMP4.
  6. "Robust stability of the embryonic axial pattern requires a secreted scaffold for chordin degradation."
    Inomata H., Haraguchi T., Sasai Y.
    Cell 134:854-865(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OLFML3.

Entry informationi

Entry nameiCHRD_XENLA
AccessioniPrimary (citable) accession number: Q91713
Secondary accession number(s): Q6DD60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3