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Protein

Chordin

Gene

chrd

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potent dorsalizing factor. Has potent axis-forming activities including the ability to recruit neighboring cells into secondary axes. Regulates cell-cell interactions in the organizing centers of head, trunk and tail development.1 Publication

GO - Molecular functioni

GO - Biological processi

  • axis specification Source: UniProtKB
  • chordate embryonic development Source: UniProtKB
  • post-anal tail morphogenesis Source: UniProtKB
  • pronephros development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Chordin
Alternative name(s):
Organizer-specific secreted-dorsalizing factor
Gene namesi
Name:chrd
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865395. chrd.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000536720 – 941ChordinAdd BLAST922

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi244N-linked (GlcNAc...)Sequence analysis1
Glycosylationi350N-linked (GlcNAc...)Sequence analysis1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Glycosylationi735N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Cleaved by bmp1; cleavage participates in dorsoventral patterning during early development. Cleavage by bmp1 is enhanced by the interaction with olfml3/ont1.1 Publication

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

First expressed at stage 9.5 in the dorsal marginal zone. Localized to the dorsal lip of the blastopore (Spemann organizer) during early gastrulation, after which expression continues in tissues derived from the organizer. Expressed in the prechordal plate (head mesoderm) and notochord during early neurulation (stage 13), transiently in the forebrain, tailbud hinge and posterior notochord in the early tailbud (stage 26), the chordoneural hinge in the late tailbud (stage 33) and finally the tip of the tail in the tadpole (stage 42).1 Publication

Inductioni

By activin in the presence of de novo protein synthesis, and by gsc and not2.2 Publications

Interactioni

Subunit structurei

Interacts with twsg1 and/or bmp4. Interacts with olfml3/ont1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
olfml3B5MFE96EBI-1997746,EBI-1997734
tll1Q8JI282EBI-1997746,EBI-1997794

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ91713. 4 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ91713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 117VWFC 1PROSITE-ProRule annotationAdd BLAST77
Domaini161 – 279CHRD 1PROSITE-ProRule annotationAdd BLAST119
Domaini281 – 401CHRD 2PROSITE-ProRule annotationAdd BLAST121
Domaini402 – 522CHRD 3PROSITE-ProRule annotationAdd BLAST121
Domaini528 – 655CHRD 4PROSITE-ProRule annotationAdd BLAST128
Domaini691 – 751VWFC 2PROSITE-ProRule annotationAdd BLAST61
Domaini769 – 838VWFC 3PROSITE-ProRule annotationAdd BLAST70
Domaini857 – 919VWFC 4PROSITE-ProRule annotationAdd BLAST63

Sequence similaritiesi

Belongs to the chordin family.Curated
Contains 4 CHRD domains.PROSITE-ProRule annotation
Contains 4 VWFC domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG005330.
KOiK04657.

Family and domain databases

InterProiIPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_dom.
[Graphical view]
PfamiPF07452. CHRD. 3 hits.
PF00093. VWC. 3 hits.
[Graphical view]
PIRSFiPIRSF002496. Chordin. 1 hit.
SMARTiSM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view]
PROSITEiPS50933. CHRD. 4 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCPPILLVW TLWIMAVDCS RPKVFLPIQP EQEPLQSKTP AGCTFGGKFY
60 70 80 90 100
SLEDSWHPDL GEPFGVMHCV LCYCEPQRSR RGKPSGKVSC KNIKHDCPSP
110 120 130 140 150
SCANPILLPL HCCKTCPKAP PPPIKKSDFV FDGFEYFQEK DDDLYNDRSY
160 170 180 190 200
LSSDDVAVEE SRSEYVALLT APSHVWPPVT SGVAKARFNL QRSNLLFSIT
210 220 230 240 250
YKWIDRLSRI RFSDLDGSVL FEHPVHRMGS PRDDTICGIW RSLNRSTLRL
260 270 280 290 300
LRMGHILVSL VTTTLSEPEI SGKIVKHKAL FSESFSALLT PEDSDETGGG
310 320 330 340 350
GLAMLTLSDV DDNLHFILML RGLSGEEGDQ IPILVQISHQ NHVIRELYAN
360 370 380 390 400
ISAQEQDFAE VLPDLSSREM LWLAQGQLEI SVQTEGRRPQ SMSGIITVRK
410 420 430 440 450
SCDTLQSVLS GGDALNPTKT GAVGSASITL HENGTLEYQI QIAGTMSTVT
460 470 480 490 500
AVTLETKPRR KTKRNILHDM SKDYHDGRVW GYWIDANARD LHMLLQSELF
510 520 530 540 550
LNVATKDFQE GELRGQITPL LYSGLWARYE KLPVPLAGQF VSPPIRTGSA
560 570 580 590 600
GHAWVSLDEH CHLHYQIVVT GLGKAEDAAL NAHLHGFAEL GEVGESSPGH
610 620 630 640 650
KRLLKGFYGS EAQGSVKDLD LELLGHLSRG TAFIQVSTKL NPRGEIRGQI
660 670 680 690 700
HIPNSCESGG VSLTPEEPEY EYEIYEEGRQ RDPDDLRKDP RACSFEGQLR
710 720 730 740 750
AHGSRWAPDY DRKCSVCSCQ KRTVICDPIV CPPLNCSQPV HLPDQCCPVC
760 770 780 790 800
EEKKEMREVK KPERARTSEG CFFDGDRSWK AAGTRWHPFV PPFGLIKCAI
810 820 830 840 850
CTCKGSTGEV HCEKVTCPKL SCTNPIRANP SDCCKQCPVE ERSPMELADS
860 870 880 890 900
MQSDGAGSCR FGRHWYPNHE RWHPTVPPFG EMKCVTCTCA EGITQCRRQE
910 920 930 940
CTGTTCGTGS KRDRCCTKCK DANQDEDEKV KSDETRTPWS F
Length:941
Mass (Da):104,947
Last modified:November 1, 1996 - v1
Checksum:i7D1C3262064C7323
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110L → G in AAH77767 (Ref. 2) Curated1
Sequence conflicti839Missing in AAH77767 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35764 mRNA. Translation: AAC42222.1.
BC077767 mRNA. Translation: AAH77767.1.
PIRiA55195.
RefSeqiNP_001081778.1. NM_001088309.1.
UniGeneiXl.3549.

Genome annotation databases

GeneIDi398045.
KEGGixla:398045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35764 mRNA. Translation: AAC42222.1.
BC077767 mRNA. Translation: AAH77767.1.
PIRiA55195.
RefSeqiNP_001081778.1. NM_001088309.1.
UniGeneiXl.3549.

3D structure databases

ProteinModelPortaliQ91713.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91713. 4 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398045.
KEGGixla:398045.

Organism-specific databases

CTDi8646.
XenbaseiXB-GENE-865395. chrd.

Phylogenomic databases

HOVERGENiHBG005330.
KOiK04657.

Miscellaneous databases

PROiQ91713.

Family and domain databases

InterProiIPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_dom.
[Graphical view]
PfamiPF07452. CHRD. 3 hits.
PF00093. VWC. 3 hits.
[Graphical view]
PIRSFiPIRSF002496. Chordin. 1 hit.
SMARTiSM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view]
PROSITEiPS50933. CHRD. 4 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHRD_XENLA
AccessioniPrimary (citable) accession number: Q91713
Secondary accession number(s): Q6DD60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 16, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.