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Q91713 (CHRD_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Chordin
Alternative name(s):
Organizer-specific secreted-dorsalizing factor
Gene names
Name:chrd
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length941 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potent dorsalizing factor. Has potent axis-forming activities including the ability to recruit neighboring cells into secondary axes. Regulates cell-cell interactions in the organizing centers of head, trunk and tail development. Ref.1

Subunit structure

Interacts with twsg1 and/or bmp4. Interacts with olfml3/ont1. Ref.5 Ref.6

Subcellular location

Secreted By similarity.

Tissue specificity

First expressed at stage 9.5 in the dorsal marginal zone. Localized to the dorsal lip of the blastopore (Spemann organizer) during early gastrulation, after which expression continues in tissues derived from the organizer. Expressed in the prechordal plate (head mesoderm) and notochord during early neurulation (stage 13), transiently in the forebrain, tailbud hinge and posterior notochord in the early tailbud (stage 26), the chordoneural hinge in the late tailbud (stage 33) and finally the tip of the tail in the tadpole (stage 42). Ref.1

Induction

By activin in the presence of de novo protein synthesis, and by gsc and not2. Ref.1 Ref.3

Post-translational modification

Cleaved by bmp1; cleavage participates in dorsoventral patterning during early development. Cleavage by bmp1 is enhanced by the interaction with olfml3/ont1.

Sequence similarities

Belongs to the chordin family.

Contains 4 CHRD domains.

Contains 4 VWFC domains.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 941922Chordin
PRO_0000005367

Regions

Domain41 – 11777VWFC 1
Domain161 – 279119CHRD 1
Domain281 – 401121CHRD 2
Domain402 – 522121CHRD 3
Domain528 – 655128CHRD 4
Domain691 – 75161VWFC 2
Domain769 – 83870VWFC 3
Domain857 – 91963VWFC 4

Amino acid modifications

Glycosylation2441N-linked (GlcNAc...) Potential
Glycosylation3501N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Glycosylation7351N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1101L → G in AAH77767. Ref.2
Sequence conflict8391Missing in AAH77767. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q91713 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7D1C3262064C7323

FASTA941104,947
        10         20         30         40         50         60 
MQCPPILLVW TLWIMAVDCS RPKVFLPIQP EQEPLQSKTP AGCTFGGKFY SLEDSWHPDL 

        70         80         90        100        110        120 
GEPFGVMHCV LCYCEPQRSR RGKPSGKVSC KNIKHDCPSP SCANPILLPL HCCKTCPKAP 

       130        140        150        160        170        180 
PPPIKKSDFV FDGFEYFQEK DDDLYNDRSY LSSDDVAVEE SRSEYVALLT APSHVWPPVT 

       190        200        210        220        230        240 
SGVAKARFNL QRSNLLFSIT YKWIDRLSRI RFSDLDGSVL FEHPVHRMGS PRDDTICGIW 

       250        260        270        280        290        300 
RSLNRSTLRL LRMGHILVSL VTTTLSEPEI SGKIVKHKAL FSESFSALLT PEDSDETGGG 

       310        320        330        340        350        360 
GLAMLTLSDV DDNLHFILML RGLSGEEGDQ IPILVQISHQ NHVIRELYAN ISAQEQDFAE 

       370        380        390        400        410        420 
VLPDLSSREM LWLAQGQLEI SVQTEGRRPQ SMSGIITVRK SCDTLQSVLS GGDALNPTKT 

       430        440        450        460        470        480 
GAVGSASITL HENGTLEYQI QIAGTMSTVT AVTLETKPRR KTKRNILHDM SKDYHDGRVW 

       490        500        510        520        530        540 
GYWIDANARD LHMLLQSELF LNVATKDFQE GELRGQITPL LYSGLWARYE KLPVPLAGQF 

       550        560        570        580        590        600 
VSPPIRTGSA GHAWVSLDEH CHLHYQIVVT GLGKAEDAAL NAHLHGFAEL GEVGESSPGH 

       610        620        630        640        650        660 
KRLLKGFYGS EAQGSVKDLD LELLGHLSRG TAFIQVSTKL NPRGEIRGQI HIPNSCESGG 

       670        680        690        700        710        720 
VSLTPEEPEY EYEIYEEGRQ RDPDDLRKDP RACSFEGQLR AHGSRWAPDY DRKCSVCSCQ 

       730        740        750        760        770        780 
KRTVICDPIV CPPLNCSQPV HLPDQCCPVC EEKKEMREVK KPERARTSEG CFFDGDRSWK 

       790        800        810        820        830        840 
AAGTRWHPFV PPFGLIKCAI CTCKGSTGEV HCEKVTCPKL SCTNPIRANP SDCCKQCPVE 

       850        860        870        880        890        900 
ERSPMELADS MQSDGAGSCR FGRHWYPNHE RWHPTVPPFG EMKCVTCTCA EGITQCRRQE 

       910        920        930        940 
CTGTTCGTGS KRDRCCTKCK DANQDEDEKV KSDETRTPWS F 

« Hide

References

« Hide 'large scale' references
[1]"Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes."
Sasai Y., Lu B., Steinbeisser H., Geissert D., Gont L.K., De Robertis E.M.
Cell 79:779-790(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Tissue: Dorsal lip.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
[3]"Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus."
Gont L.K., Fainsod A., Kim S.-H., De Robertis E.M.
Dev. Biol. 174:174-178(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[4]"Is chordin a long-range- or short-range-acting factor? Roles for BMP1-related metalloproteases in chordin and BMP4 autofeedback loop regulation."
Blitz I.L., Shimmi O., Wuennenberg-Stapleton K., O'Connor M.B., Cho K.W.Y.
Dev. Biol. 223:120-138(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE.
[5]"The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling."
Oelgeschlager M., Larrain J., Geissert D., De Robertis E.M.
Nature 405:757-763(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TWSG1 AND BMP4.
[6]"Robust stability of the embryonic axial pattern requires a secreted scaffold for chordin degradation."
Inomata H., Haraguchi T., Sasai Y.
Cell 134:854-865(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OLFML3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L35764 mRNA. Translation: AAC42222.1.
BC077767 mRNA. Translation: AAH77767.1.
PIRA55195.
RefSeqNP_001081778.1. NM_001088309.1.
UniGeneXl.3549.

3D structure databases

ProteinModelPortalQ91713.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ91713. 4 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID398045.
KEGGxla:398045.

Organism-specific databases

CTD8646.
XenbaseXB-GENE-865395. chrd.

Phylogenomic databases

HOVERGENHBG005330.
KOK04657.

Family and domain databases

InterProIPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_C.
[Graphical view]
PfamPF07452. CHRD. 4 hits.
PF00093. VWC. 3 hits.
[Graphical view]
PIRSFPIRSF002496. Chordin. 1 hit.
SMARTSM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view]
PROSITEPS50933. CHRD. 4 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHRD_XENLA
AccessionPrimary (citable) accession number: Q91713
Secondary accession number(s): Q6DD60
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families