Reviewed, UniProtKB/Swiss-Prot Q91694 (EPA4B_XENLA)

Names and origin

Protein names

Recommended name:
    Ephrin type-A receptor 4-B
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor PAG
    Pagliaccio

Gene names

Name:	epha4-B
Synonyms:	pag

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	985 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May act as a receptor for members of the ephrin-A family. May play a role in the differentiation of cranial neural crest and other tissues.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Localized expression in a subset of neural crest and neural tissues in embryos.
Developmental stage	Present transiently in visceral arch 3. Also expressed in the forebrain, rhombomeres R3 and R5 of the hindbrain and in the pronephros.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 985	965	Ephrin type-A receptor 4-B		PRO_0000016811
Regions
	Topological domain	21 – 547	527	Extracellular Potential
	Transmembrane	548 – 569	22	Potential
	Topological domain	570 – 985	416	Cytoplasmic Potential
	Domain	328 – 430	103	Fibronectin type-III 1
	Domain	440 – 533	94	Fibronectin type-III 2
	Domain	620 – 881	262	Protein kinase
	Domain	910 – 974	65	SAM
	Nucleotide binding	626 – 634	9	ATP By similarity
	Motif	983 – 985	3	PDZ-binding Potential
	Compositional bias	191 – 325	135	Cys-rich
Sites
	Active site	745	1	Proton acceptor By similarity
	Binding site	652	1	ATP By similarity
Amino acid modifications
	Modified residue	595	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	601	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	778	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	927	1	Phosphotyrosine; by autocatalysis Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	407	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q91694-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B6CFD128ACB64DF0	FASTA	985	109,730
	10 20 30 40 50 60 MAGIVHGILF CGLFGLCWAV TGSRIYPASE VTLLDSRSVQ GELGWIASPL EGGWEEVSIM 70 80 90 100 110 120 DEKNTPIRTY QVCNVMESSQ NNWLRTDWIP RSGAQRVYVE IKFTLRDCNS LPGVMGTCKE 130 140 150 160 170 180 TFNLYYYESN NDKERFIRET QYVKIDTIAA DESFTQVDIG DRIMKLNTEV RDVGPLSKKG 190 200 210 220 230 240 FYLAFQDVGA CIALVSVRVF YKKCPLTVRN LAQFPDTITG SDTSSLVEVR GSCVDNSEEK 250 260 270 280 290 300 DVPKMYCGAD GEWLVPIGNC LCNAGFEEHN GGCQACKVGY YKALSTDAAC SKCPPHSYAL 310 320 330 340 350 360 REGSTSCTCD RGYFRADTDP ASMPCTRPPS APQNLISNVN ETSVNLEWSP PQNSGGRPDV 370 380 390 400 410 420 SYNLVCKRCG SDLTRCSPCG SGVHYSPQQN GLKTTKVSIN DLQAHTNYTF EVWAINGVSK 430 440 450 460 470 480 QNPEQDQAVS VTVTTNQAAP STVTQIQPKE ITRHSVSLTW PEPERANGVI LEYEVKYYEK 490 500 510 520 530 540 DQNERSYRIV KTASRSADIK GLNPLTGYVF HVRARTAAGY GEFSGPFEFT TNTVPSPMIG 550 560 570 580 590 600 EGTSPTVLLV SVAGSIVLVV ILIAAFVISR RRSKYSKAKQ EADEEKHLNQ GVKTYVDPFT 610 620 630 640 650 660 YEDPNQAVRE FAKEIDASCI KIEKVIGVGE FGEVCSGRLK VPGKREIYVA IKTLKAGYTD 670 680 690 700 710 720 KQRRDFLSEA SIMGQFDHPN IIHLEGVVTK CKPVMIITEY MENGSLDAFL RKNDGRFTVI 730 740 750 760 770 780 QLVGMLRGIG SGMKYLSDMS YVHRDLAARN ILVNSNLVCK VSDFGMSRVL EDDPEAAYTT 790 800 810 820 830 840 RGGKIPIRWT APEAIAYRKF TSASDVWSYG IVMWEVMSYG ERPYWDMSNQ DVIKAIEEGY 850 860 870 880 890 900 RLPPPMDCPI ALHQLMLDCW QKDRSDRPKF GQIVSMLDKL IRNPNSLKRT GLENSRTNTA 910 920 930 940 950 960 LLDPSSPEWS QVASVLDWLQ ASKWKRYKDN FTAAGYTSLE AVVHVNQDDL TRIGISSPSH 970 980 QNKILSSVQG MRTQLQQMQG RMVPV

References

[1]

"Pagliaccio, a member of the Eph family of receptor tyrosine kinase genes, has localized expression in a subset of neural crest and neural tissues in Xenopus laevis embryos." Winning R.S., Sargent T.D. Mech. Dev. 46:219-229(1994) [PubMed: 7918105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Neural crest.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L26099 mRNA. Translation: AAA64464.1.
PIR	I51549.
UniGene	Xl.13
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
SMR	Q91694. Positions 612-887, 909-980.
ModBase	Search...
Organism-specific databases
Xenbase	XB-FEAT-1015802. epha4.
Phylogenomic databases
HOVERGEN	Q91694.
Enzyme and pathway databases
BRENDA	2.7.10.1. 648.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR011510. SAM_2. IPR013761. SAM_type. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016257. TyrPK_ephrin_receptor. IPR001426. YKase_receptorV_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07714. Pkinase_Tyr. 1 hit. PF07647. SAM_2. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII. PR00109. TYRKINASE.
ProDom	PD001495. Ephrin_receptor. 1 hit. PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPA4B_XENLA

Accession

Primary (citable) accession number: Q91694

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project