Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger protein ZIC 2-A

Gene

zic2-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional repressor that inhibits neurogenesis and induces neural and neural crest differentiation. Regulates anteroposterior patterning in early development by inhibiting expression of the nodal genes through the inhibition of vegt. Required for gastrulation movements and for proper anterior neural and axial development. May also act as a transcriptional activator. May bind to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri277 – 31236C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri321 – 34828C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri354 – 37825C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri384 – 40825C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri414 – 43623C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: UniProtKB
  • cell migration involved in gastrulation Source: UniProtKB
  • negative regulation of neurogenesis Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • neural crest cell fate commitment Source: UniProtKB
  • neural crest formation Source: UniProtKB
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • visual perception Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Gastrulation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ZIC 2-A
Alternative name(s):
Zinc finger DNA-binding protein fZic
Zinc finger protein ZIC 2
Short name:
XlZic2
Short name:
xZic2
Zinc finger protein of the cerebellum 2-A
Gene namesi
Name:zic2-a
Synonyms:zic2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-482658. zic2.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Zinc finger protein ZIC 2-APRO_0000047249Add
BLAST

Expressioni

Tissue specificityi

Expressed in both animal and vegetal regions of the oocyte. At blastula and gastrula stages (stages 9 to 10.5), expressed throughout the ectoderm. During late gastrula to neurula stages, expression gradually diminishes in the midline region of the neural plate and increases in the anterior neural folds, and continues to be expressed in the posterior medial part of the neural plate. In early tailbud stages (stages 22-23), expressed in the dorsal forebrain, midbrain and hindbrain. Subsequently expressed in the telencephalon and diencephalon/mesencephalon boundary. In the spinal cord, expression is restricted to the dorsal most region including the roof plate. Also expressed in the somites and eye vesicles. In the eye, expression is restricted to the ciliary marginal zone of neural retina and is absent from the lens.4 Publications

Developmental stagei

Expressed both maternally and zygotically. Continuously expressed from the egg to the tailbud stage (stage 30) with an increase in expression at the early gastrula stage (stage 10).3 Publications

Inductioni

By zic1, zic2, zic3 and zic5.1 Publication

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi20 – 289Poly-His
Compositional biasi29 – 3911Poly-AlaAdd
BLAST
Compositional biasi67 – 16296Gly-richAdd
BLAST
Compositional biasi165 – 26096His-richAdd
BLAST
Compositional biasi476 – 4794Poly-Ala

Domaini

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.By similarity

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri277 – 31236C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri321 – 34828C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri354 – 37825C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri384 – 40825C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri414 – 43623C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG007135.
KOiK06235.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLDAGPQFP ALGVGTFARH HHHHHHHHAA VAAAAAAAAE MQERELSLAQ
60 70 80 90 100
NTFVEPTHMA AFKLNPGGGS GGGSGGGGGG GGAGPNGGAG ASGPHDLSPP
110 120 130 140 150
GQTSAFTSQA GYPTSALAPH SAYSGAAAFN SPRDFLFRGR GFAEGSAAAG
160 170 180 190 200
GGQHGLFGPP AGSLHHHPHH HHQLSHAEHP QGHLLFPGIH DQHAAASQNT
210 220 230 240 250
LGGQMRLGLP GEVFGRTEQY RQVSSPRGDP YTAAQLHNQY SPMNMGMNMA
260 270 280 290 300
AHHHHHHHHH PGAFFRYMRQ PCIKQELICK WIDPEQLNNP KKSCTKTFST
310 320 330 340 350
MHELVTHVSV EHVGGPEQSN HICFWEECPR EGKPFKAKYK LVNHIRVHTG
360 370 380 390 400
EKPFPCPFPG CGKVFARSEN LKIHKRTHTG EKPFQCEFEG CDRRFANSSD
410 420 430 440 450
RKKHMHVHTS DKPYLCKMCD KSYTHPSSLR KHMKVHESSP QGSESSPAAS
460 470 480 490 500
SGYESSTPPG LVSPNSETQN PNLSPAAAAV SAVHNVSSGA SGALASNFNE

WYV
Length:503
Mass (Da):53,941
Last modified:February 10, 2009 - v2
Checksum:i3F173F6648C8E806
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 212Missing in BAA33407 (PubMed:9739105).Curated
Sequence conflicti32 – 332Missing in AAH82436 (Ref. 3) Curated
Sequence conflicti60 – 601A → G in BAA33407 (PubMed:9739105).Curated
Sequence conflicti75 – 762Missing in AAC80229 (PubMed:9634234).Curated
Sequence conflicti422 – 4221S → T in BAA33407 (PubMed:9739105).Curated
Sequence conflicti438 – 4381S → T in BAA33407 (PubMed:9739105).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009565 mRNA. Translation: BAA33407.1.
U57453 mRNA. Translation: AAC80229.1.
BC082436 mRNA. Translation: AAH82436.1.
RefSeqiNP_001081193.1. NM_001087724.1.
UniGeneiXl.1085.

Genome annotation databases

GeneIDi397704.
KEGGixla:397704.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009565 mRNA. Translation: BAA33407.1.
U57453 mRNA. Translation: AAC80229.1.
BC082436 mRNA. Translation: AAH82436.1.
RefSeqiNP_001081193.1. NM_001087724.1.
UniGeneiXl.1085.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397704.
KEGGixla:397704.

Organism-specific databases

CTDi397704.
XenbaseiXB-GENE-482658. zic2.

Phylogenomic databases

HOVERGENiHBG007135.
KOiK06235.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Xenopus Zic family and its role in neural and neural crest development."
    Nakata K., Nagai T., Aruga J., Mikoshiba K.
    Mech. Dev. 75:43-51(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Gli/Zic factors pattern the neural plate by defining domains of cell differentiation."
    Brewster R., Lee J., Ruiz i Altaba A.
    Nature 393:579-583(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Neurula.
  3. NIH - Xenopus Gene Collection (XGC) project
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Oocyte.
  4. "Maternal Xenopus Zic2 negatively regulates Nodal-related gene expression during anteroposterior patterning."
    Houston D.W., Wylie C.
    Development 132:4845-4855(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 226-302, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  5. "A novel member of the Xenopus Zic family, Zic5, mediates neural crest development."
    Nakata K., Koyabu Y., Aruga J., Mikoshiba K.
    Mech. Dev. 99:83-91(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Xenopus Zic4: conservation and diversification of expression profiles and protein function among the Xenopus Zic family."
    Fujimi T.J., Mikoshiba K., Aruga J.
    Dev. Dyn. 235:3379-3386(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiZIC2A_XENLA
AccessioniPrimary (citable) accession number: Q91689
Secondary accession number(s): O93487, Q641A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 10, 2009
Last modified: October 14, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.