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Protein

Nodal homolog

Gene

nodal

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cooperation and regulatory loops of multiple nodals are essential for mesendoderm patterning in early embryos. Essential for mesoderm formation and axial patterning during embryonic development. Activates the activin-like signaling pathway to induce dorsal and ventral mesoderm in animal cap ectoderm. In addition, also dorsalizes ventral marginal zone (VMZ) tissues during gastrulation. Acts in a downstream signaling cascade via cripto and cer1 to mediate cardiogenesis in embryonic mesoderm. Directs the orientation of the left-right axis by driving the left-specific gene cascade in the left lateral plate mesoderm.7 Publications

GO - Molecular functioni

  • morphogen activity Source: BHF-UCL

GO - Biological processi

  • axial mesoderm development Source: UniProtKB
  • axial mesoderm formation Source: UniProtKB
  • determination of left/right symmetry Source: UniProtKB
  • growth Source: InterPro
  • heart development Source: UniProtKB
  • paraxial mesoderm development Source: BHF-UCL
  • positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Nodal homolog
Alternative name(s):
Nodal-related protein 1
Xnr-1
Short name:
Xnr1
Short name:
nr-1
Gene namesi
Name:nodalBy similarity
Synonyms:nr11 Publication
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865681. nodal1.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Propeptidei19 – 281263Sequence analysisPRO_0000273274Add
BLAST
Chaini282 – 406125Nodal homologSequence analysisPRO_0000273275Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence analysis
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence analysis
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence analysis
Disulfide bondi306 ↔ 372By similarity
Disulfide bondi335 ↔ 403By similarity
Disulfide bondi339 ↔ 405By similarity
Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence analysis
Disulfide bondi369 – 369InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

In the first phase of expression, localized to the vegetal region of the blastula. During gastrulation (stage 10.5), this expression disappears and instead becomes localized to the dorsal marginal zone, with enrichment in the organizer. During the second phase of expression in neurulae and tailbud embryos, expression restarts firstly in two symmetric patches near the posterior end of the notochord, and then in a large asymmetrical domain in the left lateral plate mesoderm.4 Publications

Developmental stagei

Has two phases of temporal expression during embryogenesis; first expressed at late blastula (stage 9) with expression peaking at early gastrula. Expression then disappears before restarting in a second phase in late neurulae (stage 17).1 Publication

Inductioni

By dorsal mesoderm-inducing signals including smad2-smad4, activin and other nodal-related proteins including nodal2/nr-2 and nodal4/nr-4. Shows autoinduction by nodal/nr-1. Induced by vegt, acting in an autoregulatory loop. Beta-catenin potentiates the response to activin. Not induced by wnt8 alone, but wnt8 potentiates the response to activin. Suppressed by ventral inducers such as bmp4.7 Publications

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Interacts with, and is inhibited by cer1 and gdf10/bmp3b.By similarity2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ91619.
SMRiQ91619. Positions 368-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG108195.
KOiK04666.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 2 hits.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLTAVLYL GFACISQGLP TWPDRVESRN PLFGSRVALN LPSLLDGNRH
60 70 80 90 100
HRDMRYPLYM MQLYQNLVTG NDTGLANRPN TATKEYDTVL SLFAKKCTES
110 120 130 140 150
ENRWTLSFDM SAVSRSNELK LAELRILLPH TEPSHNITMD MYHSRDGEDN
160 170 180 190 200
LYLGSFNANP PSTKGSPWKV FNVTKILQPY FKERRDIDSE HLKAKERAER
210 220 230 240 250
GSGMSNAEFI DAPGPSQQYN PHQTSVPTYL NTKGVMLVLF TKVKSSANHI
260 270 280 290 300
GFPSLIKTAE SSKYVDIEKA SRVPGIRRHR RNRNENHHLS IGSIPSRHVE
310 320 330 340 350
NGKPLCRRVD MIVDFEDIGW SSWIVYPKKY NAYRCEGACP IPLNETFKPT
360 370 380 390 400
NHAYMKSVVK LYQPERVECP LCVPVKMSPL SMLYYEGDEV VLRHHQEMIV

EECGCS
Length:406
Mass (Da):46,332
Last modified:November 1, 1996 - v1
Checksum:i3AFB1D98BCDEAE70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29447 mRNA. Translation: AAA97392.1.
AF410903 Genomic DNA. Translation: AAL05845.1.
AF410904 Genomic DNA. Translation: AAL05846.1.
RefSeqiNP_001079265.1. NM_001085796.1.
UniGeneiXl.1037.

Genome annotation databases

GeneIDi378543.
KEGGixla:378543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29447 mRNA. Translation: AAA97392.1.
AF410903 Genomic DNA. Translation: AAL05845.1.
AF410904 Genomic DNA. Translation: AAL05846.1.
RefSeqiNP_001079265.1. NM_001085796.1.
UniGeneiXl.1037.

3D structure databases

ProteinModelPortaliQ91619.
SMRiQ91619. Positions 368-406.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi378543.
KEGGixla:378543.

Organism-specific databases

CTDi378543.
XenbaseiXB-GENE-865681. nodal1.

Phylogenomic databases

HOVERGENiHBG108195.
KOiK04666.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 2 hits.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNODAL_XENLA
AccessioniPrimary (citable) accession number: Q91619
Secondary accession number(s): Q90XB4, Q90XB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.