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Protein

Insulin receptor substrate 1-A

Gene

irs1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or grb2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin receptor substrate 1-A
Short name:
IRS1-A
Short name:
xIRS-1-A
Alternative name(s):
XIRS-L
Gene namesi
Name:irs1-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-478676. irs1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 885885Insulin receptor substrate 1-ACuratedPRO_0000223524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei104 – 1041PhosphoserineBy similarity
Modified residuei257 – 2571Phosphotyrosine; by INSRBy similarity
Modified residuei364 – 3641Phosphotyrosine; by INSRBy similarity
Modified residuei381 – 3811Phosphotyrosine; by INSRBy similarity
Modified residuei409 – 4091PhosphotyrosineBy similarity
Modified residuei582 – 5821Phosphotyrosine; by INSRBy similarity
Modified residuei620 – 6201Phosphotyrosine; by INSRBy similarity
Modified residuei672 – 6721Phosphotyrosine; by INSRBy similarity
Modified residuei834 – 8341Phosphotyrosine; by INSRBy similarity
Modified residuei866 – 8661Phosphotyrosine; by INSRBy similarity

Post-translational modificationi

Phosphorylation of Tyr-582 is required for grb2-binding.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed in stage VI primed oocytes (at protein level).1 Publication

Interactioni

Subunit structurei

Interacts with the NPXY motif of tyrosine-phosphorylated igf1r and insr via the PTB domain (By similarity). Binds to phosphatidylinositol 3-kinase p85 subunit at a low level in vitro prior to phosphorylation. Binding is greatly enhanced following tyrosine phosphorylation by insr and probably occurs via the phosphorylated YXXM motifs.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-1532316.

Structurei

3D structure databases

ProteinModelPortaliQ91615.
SMRiQ91615. Positions 1-59.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5656IRS-type PTBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni582 – 5843GRB2-bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi257 – 2604YXXM motif 1Sequence analysis
Motifi318 – 3214YXXM motif 2Sequence analysis
Motifi364 – 3674YXXM motif 3Sequence analysis
Motifi381 – 3844YXXM motif 4Sequence analysis
Motifi409 – 4124YXXM motif 5Sequence analysis
Motifi451 – 4544YXXM motif 6Sequence analysis
Motifi620 – 6234YXXM motif 7Sequence analysis
Motifi672 – 6754YXXM motif 8Sequence analysis
Motifi706 – 7094YXXM motif 9Sequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi114 – 237124Ser-richSequence analysisAdd
BLAST
Compositional biasi517 – 52610Poly-SerSequence analysis

Sequence similaritiesi

Contains 1 IRS-type PTB domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG000542.
KOiK16172.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR002404. IRS_PTB.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF02174. IRS. 1 hit.
[Graphical view]
PRINTSiPR00628. INSULINRSI.
SMARTiSM00310. PTBI. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS51064. IRS_PTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIRRCGHSE NFFFIEVGRS AVTGAGEFWM QVDDSVVAQN MHETILEAMK
60 70 80 90 100
ALSDEFRPRS KSQSSSNCSN PISVPLRRHH LNHPPPSQVG LNRRARTESA
110 120 130 140 150
TATSPAGGAA KHGSSSFRVR ASSDGEGTMS RPASMEGSPV SPSASRTQSH
160 170 180 190 200
RHRGSSRLHP PLNHSRSIPM PATRCSPSAT SPVSLSSSST SGHGSTSDCM
210 220 230 240 250
CPRRSSASIS GSPSDGGFIS SDEYGSSPCD FRSSFRSVTP DSMGHTPPAR
260 270 280 290 300
EEELNNYICM GKSSSHLQRG PQQRYQPSRG EELTDFDKVF RKRTHSSGTS
310 320 330 340 350
PPTVSHQKTP SQSSIEEYTE MMPTHPVRLT SFRHSAFVPT YSYPEECLDL
360 370 380 390 400
HLEGSRANHK DDGYMPMSPG VAPVSTKTND YMPMSPKSVS APQQIINPRW
410 420 430 440 450
HSAVDSNGYM MMSPSGSCSP DNTNYSKIWT NGTNPKLSID SIEGKLPCSD
460 470 480 490 500
YINMSPASGS TTSTPPDSYL NSVEESTKPV YSYFSLPRSF KHVHRKSEDG
510 520 530 540 550
NLRISANSGH NLYTEDSSSS STSSDSLGGQ DPQQPRKGEG CIQGKRLTRP
560 570 580 590 600
TRLSLENSSK ASTLPRVREP ALPPEPKSPG EYVNIEFNDK VFSGGLVPSM
610 620 630 640 650
CSLPFVQSRV VPQRENLSEY MNMDLGVWRA KTSYASTSSY EPPNKPVNSV
660 670 680 690 700
CPTETCSSSR PPIRGKPISR DYMSMQLGSL CPDYSQVPPT RLSAKSITLS
710 720 730 740 750
SSKSNYAEMS SGRVSDNIPA IAPASNSSLS EASRSSLLGQ GSGPSAFTRV
760 770 780 790 800
SLSPNRNPSA KVIRAGDPQG RRRHSSETFS STPTTARVTT GPVSGEDVKR
810 820 830 840 850
HSSASFENVW LRPGEIARRD SLQPSDHTHN GLNYIDLDLA KDLSSLDHCN
860 870 880
SHQSGVSHPS DDLSPYASIT FHKLEEHRSQ AETEE
Length:885
Mass (Da):96,146
Last modified:November 1, 1996 - v1
Checksum:i9F1B96C64949CA7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27842 mRNA. Translation: AAA73572.1.
RefSeqiNP_001084092.1. NM_001090623.1.
UniGeneiXl.957.

Genome annotation databases

GeneIDi399299.
KEGGixla:399299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27842 mRNA. Translation: AAA73572.1.
RefSeqiNP_001084092.1. NM_001090623.1.
UniGeneiXl.957.

3D structure databases

ProteinModelPortaliQ91615.
SMRiQ91615. Positions 1-59.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1532316.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399299.
KEGGixla:399299.

Organism-specific databases

CTDi3667.
XenbaseiXB-GENE-478676. irs1.

Phylogenomic databases

HOVERGENiHBG000542.
KOiK16172.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR002404. IRS_PTB.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF02174. IRS. 1 hit.
[Graphical view]
PRINTSiPR00628. INSULINRSI.
SMARTiSM00310. PTBI. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS51064. IRS_PTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of an amphibian insulin receptor substrate 1-like cDNA and involvement of phosphatidylinositol 3-kinase in insulin-induced Xenopus oocyte maturation."
    Liu X.J., Sorisky A., Zhu L., Pawson T.
    Mol. Cell. Biol. 15:3563-3570(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, PHOSPHORYLATION, INTERACTION WITH PIK3R1.
    Tissue: Ovary1 Publication.

Entry informationi

Entry nameiIRS1A_XENLA
AccessioniPrimary (citable) accession number: Q91615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.