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Protein

Insulin receptor substrate 1-A

Gene

irs1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or grb2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin receptor substrate 1-A
Short name:
IRS1-A
Short name:
xIRS-1-A
Alternative name(s):
XIRS-L
Gene namesi
Name:irs1-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-478676 irs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002235241 – 885Insulin receptor substrate 1-ACuratedAdd BLAST885

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104PhosphoserineBy similarity1
Modified residuei257Phosphotyrosine; by INSRBy similarity1
Modified residuei364Phosphotyrosine; by INSRBy similarity1
Modified residuei381Phosphotyrosine; by INSRBy similarity1
Modified residuei409PhosphotyrosineBy similarity1
Modified residuei582Phosphotyrosine; by INSRBy similarity1
Modified residuei620Phosphotyrosine; by INSRBy similarity1
Modified residuei672Phosphotyrosine; by INSRBy similarity1
Modified residuei834Phosphotyrosine; by INSRBy similarity1
Modified residuei866Phosphotyrosine; by INSRBy similarity1

Post-translational modificationi

Phosphorylation of Tyr-582 is required for grb2-binding.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed in stage VI primed oocytes (at protein level).1 Publication

Interactioni

Subunit structurei

Interacts with the NPXY motif of tyrosine-phosphorylated igf1r and insr via the PTB domain (By similarity). Binds to phosphatidylinositol 3-kinase p85 subunit at a low level in vitro prior to phosphorylation. Binding is greatly enhanced following tyrosine phosphorylation by insr and probably occurs via the phosphorylated YXXM motifs.By similarity1 Publication

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ91615
SMRiQ91615
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 56IRS-type PTBPROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni582 – 584GRB2-bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi257 – 260YXXM motif 1Sequence analysis4
Motifi318 – 321YXXM motif 2Sequence analysis4
Motifi364 – 367YXXM motif 3Sequence analysis4
Motifi381 – 384YXXM motif 4Sequence analysis4
Motifi409 – 412YXXM motif 5Sequence analysis4
Motifi451 – 454YXXM motif 6Sequence analysis4
Motifi620 – 623YXXM motif 7Sequence analysis4
Motifi672 – 675YXXM motif 8Sequence analysis4
Motifi706 – 709YXXM motif 9Sequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi114 – 237Ser-richSequence analysisAdd BLAST124
Compositional biasi517 – 526Poly-SerSequence analysis10

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG000542
KOiK16172

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
PfamiView protein in Pfam
PF02174 IRS, 1 hit
PRINTSiPR00628 INSULINRSI
SMARTiView protein in SMART
SM00310 PTBI, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit

Sequencei

Sequence statusi: Complete.

Q91615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIRRCGHSE NFFFIEVGRS AVTGAGEFWM QVDDSVVAQN MHETILEAMK
60 70 80 90 100
ALSDEFRPRS KSQSSSNCSN PISVPLRRHH LNHPPPSQVG LNRRARTESA
110 120 130 140 150
TATSPAGGAA KHGSSSFRVR ASSDGEGTMS RPASMEGSPV SPSASRTQSH
160 170 180 190 200
RHRGSSRLHP PLNHSRSIPM PATRCSPSAT SPVSLSSSST SGHGSTSDCM
210 220 230 240 250
CPRRSSASIS GSPSDGGFIS SDEYGSSPCD FRSSFRSVTP DSMGHTPPAR
260 270 280 290 300
EEELNNYICM GKSSSHLQRG PQQRYQPSRG EELTDFDKVF RKRTHSSGTS
310 320 330 340 350
PPTVSHQKTP SQSSIEEYTE MMPTHPVRLT SFRHSAFVPT YSYPEECLDL
360 370 380 390 400
HLEGSRANHK DDGYMPMSPG VAPVSTKTND YMPMSPKSVS APQQIINPRW
410 420 430 440 450
HSAVDSNGYM MMSPSGSCSP DNTNYSKIWT NGTNPKLSID SIEGKLPCSD
460 470 480 490 500
YINMSPASGS TTSTPPDSYL NSVEESTKPV YSYFSLPRSF KHVHRKSEDG
510 520 530 540 550
NLRISANSGH NLYTEDSSSS STSSDSLGGQ DPQQPRKGEG CIQGKRLTRP
560 570 580 590 600
TRLSLENSSK ASTLPRVREP ALPPEPKSPG EYVNIEFNDK VFSGGLVPSM
610 620 630 640 650
CSLPFVQSRV VPQRENLSEY MNMDLGVWRA KTSYASTSSY EPPNKPVNSV
660 670 680 690 700
CPTETCSSSR PPIRGKPISR DYMSMQLGSL CPDYSQVPPT RLSAKSITLS
710 720 730 740 750
SSKSNYAEMS SGRVSDNIPA IAPASNSSLS EASRSSLLGQ GSGPSAFTRV
760 770 780 790 800
SLSPNRNPSA KVIRAGDPQG RRRHSSETFS STPTTARVTT GPVSGEDVKR
810 820 830 840 850
HSSASFENVW LRPGEIARRD SLQPSDHTHN GLNYIDLDLA KDLSSLDHCN
860 870 880
SHQSGVSHPS DDLSPYASIT FHKLEEHRSQ AETEE
Length:885
Mass (Da):96,146
Last modified:November 1, 1996 - v1
Checksum:i9F1B96C64949CA7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27842 mRNA Translation: AAA73572.1
RefSeqiNP_001084092.1, NM_001090623.1
UniGeneiXl.957

Genome annotation databases

GeneIDi399299
KEGGixla:399299

Similar proteinsi

Entry informationi

Entry nameiIRS1A_XENLA
AccessioniPrimary (citable) accession number: Q91615
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health