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Protein

Serine/threonine-protein kinase stk11

Gene

stk11

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81ATP1
Active sitei179Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 66ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle, DNA damage

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase stk11 (EC:2.7.11.1)
Alternative name(s):
Liver kinase B1 homolog
Short name:
lkb1
Serine/threonine-protein kinase XEEK1
Gene namesi
Name:stk11
Synonyms:eek1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-955285. stk11.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81K → I: Loss of activity. 1 Publication1
Mutagenesisi192T → A: Loss of autophosphorylation. 1 Publication1

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867001 – 432Serine/threonine-protein kinase stk11Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192Phosphothreonine; by autocatalysis1 Publication1
Modified residuei427Phosphoserine; by PKASequence analysis1

Post-translational modificationi

Phosphorylated by a cAMP-dependent protein kinase. Autophosphorylated in a reaction that prefers Mn2+ to Mg2+.1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiQ91604.

Expressioni

Tissue specificityi

Oocytes, eggs and early embryos.

Developmental stagei

Its expression peaks in the oocyte and unfertilized egg, begins to decrease gradually after fertilization, and disappears during the gastrulation stage.

Interactioni

Subunit structurei

Catalytic component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi100426. 1 interactor.
IntActiQ91604. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ91604.
SMRiQ91604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 312Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG054467.
KOiK07298.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCPSSMDEE GSEEIGFLGD LSVGMDTFIH RIDSTEVIYQ PRRKRAKLVG
60 70 80 90 100
KYLMGDLLGE GSYGKVKEML DSDTLCRRAV KILKKKKLRR IPNGEANVKK
110 120 130 140 150
EIQLLRRLRH RNVIQLVDVL YNEEKQKMYM VMEYCVCGMQ EMLDSVQDKH
160 170 180 190 200
FPVFQAHGYF CQLIDGLEYL HSQGIVHKDI KPGNLLLTTD GTLKISDLGV
210 220 230 240 250
AEALHPFAEG DTCRTSQGSP AFQPPEIANG LDTFSGFKVD IWSAGVTLYN
260 270 280 290 300
ITTGLYPFEG DNIYKLFENI GKGDYSIPEE CGPLLSDLLR GMLEYDPAKR
310 320 330 340 350
FSIQQIRQHN WFRKKHPHMD PIVPIPPSPE TKDRWRSLTV VPYLEDLHGY
360 370 380 390 400
SEEEDLCDFE DDIIYTQDFT VPGQVAEDDY FAQTQSTAPS KQLCMNGTES
410 420 430
QLKTERRVSS SSQRKASTTG SKVRKLSACK QQ
Length:432
Mass (Da):49,073
Last modified:November 1, 1996 - v1
Checksum:iF4D98A06C52560F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24435 mRNA. Translation: AAC59904.1.
BC077243 mRNA. Translation: AAH77243.1.
RefSeqiNP_001083758.1. NM_001090289.1.
UniGeneiXl.16657.

Genome annotation databases

GeneIDi399100.
KEGGixla:399100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24435 mRNA. Translation: AAC59904.1.
BC077243 mRNA. Translation: AAH77243.1.
RefSeqiNP_001083758.1. NM_001090289.1.
UniGeneiXl.16657.

3D structure databases

ProteinModelPortaliQ91604.
SMRiQ91604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi100426. 1 interactor.
IntActiQ91604. 1 interactor.

PTM databases

iPTMnetiQ91604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399100.
KEGGixla:399100.

Organism-specific databases

CTDi6794.
XenbaseiXB-GENE-955285. stk11.

Phylogenomic databases

HOVERGENiHBG054467.
KOiK07298.

Enzyme and pathway databases

BRENDAi2.7.11.1. 6725.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTK11_XENLA
AccessioniPrimary (citable) accession number: Q91604
Secondary accession number(s): Q6DE91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.