Reviewed, UniProtKB/Swiss-Prot Q91571 (EPB1A_XENLA)

Names and origin

Protein names

Recommended name:
    Ephrin type-B receptor 1-A
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor XEK

Gene names

Name:	ephb1-A
Synonyms:	xek

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	985 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-B family. May play a role in the development or function of the central nervous system.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Developmental stage	Maternally expressed, it decreases at mid blastula transition and reappears at late neurulation. Expressed at higher levels in the anterior and dorsal regions of embryonic stages 16, 24 and 37. In adult it appears to be ubiquitously expressed with higher expression in brain and ovary. Expression in the brain, brachial arches, trigeminal facial ganglion, and the retina of swimming tadpole stage of development.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 985	966	Ephrin type-B receptor 1-A		PRO_0000016826
Regions
	Topological domain	20 – 542	523	Extracellular Potential
	Transmembrane	543 – 563	21	Potential
	Topological domain	564 – 985	422	Cytoplasmic Potential
	Domain	325 – 426	102	Fibronectin type-III 1
	Domain	436 – 527	92	Fibronectin type-III 2
	Domain	620 – 883	264	Protein kinase
	Domain	912 – 976	65	SAM
	Nucleotide binding	626 – 634	9	ATP By similarity
	Motif	983 – 985	3	PDZ-binding Potential
	Compositional bias	185 – 321	137	Cys-rich
Sites
	Active site	745	1	Proton acceptor By similarity
	Binding site	652	1	ATP By similarity
Amino acid modifications
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	428	1	N-linked (GlcNAc...) Potential
	Glycosylation	482	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q91571-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BE72CD1BFF51E623	FASTA	985	110,104
	10 20 30 40 50 60 MELNVLLLLL CLSGGQVGAV EETLMDTRTA TAELGWTANP SSGWEEVSGY DENLNTIRTY 70 80 90 100 110 120 QVCNVFGPKQ NNWLLTTFIP RRGAHRVYVE MRFTVRDCSS LPNVPGSCKE TFNLYYYETD 130 140 150 160 170 180 SNIENKISTF WNESPYLKVD TIAADESFSQ VDFGGRLMKV NTEVRSFGPL TRSGFYLAFQ 190 200 210 220 230 240 DYGACMSLLS VRVFFKEMPS VVQNLLVFPE TMTGAESTSL VIARGTCIPN AEEVDVPIKL 250 260 270 280 290 300 YCNGDGEWMV PIGKCTCKAG YEPENHVVCK ACPAAMFKAN QGMGICAQCP ANSRSTSEAS 310 320 330 340 350 360 PICICRNGYY RADFDTPEAP CTSVPSGPRN VISIVNETAI TLEWHPPRET GGRDDVDYNI 370 380 390 400 410 420 VCKKCRADRR ACSRCDDNVD FVPRQLGLTD TRVFISNLWA HTPYTFETQA VNGVTNKSPF 430 440 450 460 470 480 PPQHVSVNIT TNQAAPSSVP IMHQVKATMK SITLSWPQQE QPNGIILDYE IRYYEKDHHE 490 500 510 520 530 540 FNSSLARSQT NTARRTGGRV WMFMSVQVRA RTVAGYGKFS SKCGFQTLTA EDYKSELREQ 550 560 570 580 590 600 LPLTGSAAAG VVFIVSLVAI SIVCSRKRTY SKEAVYSDKL QHYSTGRGSP GMKIYIDPFT 610 620 630 640 650 660 YEDPNEAVRE FAKEIDVSFV KIEEVIGAGE FGEVYKGRLK LPSKREISVA IKTLKAGYSE 670 680 690 700 710 720 KQRRDFLSEA SIMGQFDHPN IIRLEGVVTK SRPVMIITEF MENGALDSFL RQNDGQFTVI 730 740 750 760 770 780 QLVGMLRGIA AGMKYLSEMN YVHRDLAARN ILVNSNLVCK VSDFGLSRYL QDDTSDPTYT 790 800 810 820 830 840 SSLGGKIPVR WTAQEAIAYR KFTSASDVWS YGIVMWEVMS YGERPYWTMS NQDVINAIEQ 850 860 870 880 890 900 DYRLPPPMDC PAALHQLMLD CWQKDRNSRP RLAEIVNTLR PMIRNPASLK TVATIPAVPS 910 920 930 940 950 960 QPLLDRSIPD ISAFTSVDDW LSAIKMGQYR DNFLSSGFTS LQLVAQMTSE DLLRIGITLA 970 980 GHQKKILNSI QSMRVQITQS PTSIA

References

[1]	"Expression of an amphibian homolog of the Eph family of receptor tyrosine kinases is developmentally regulated." Jones T.L., Karavanova I., Maeno M., Ong R.C., Kung H.-F., Daar I.O. Oncogene 10:1111-1117(1995) [PubMed: 7700636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14164 mRNA. Translation: AAA74888.1.
PIR	I51672.
RefSeq	NP_001084070.1.
UniGene	Xl.1028
3D structure databases
SMR	Q91571. Positions 20-199, 324-532, 593-890, 622-970, 901-985.
ModBase	Search...
Genome annotation databases
GeneID	399288.
KEGG	xla:399288.
Organism-specific databases
CTD	399288.
Xenbase	XB-FEAT-488625. ephb1.
Phylogenomic databases
HOVERGEN	Q91571.
Enzyme and pathway databases
BRENDA	2.7.10.1. 648.
Family and domain databases
InterPro	IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR011510. SAM_2. IPR010993. SAM_homology. IPR013761. SAM_type. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR016257. Tyr_prot_kinase_ephrin_rcpt. IPR001426. Tyr_prot_kinase_rcpt_V_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07647. SAM_2. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. False negative. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPB1A_XENLA

Accession

Primary (citable) accession number: Q91571

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project