Reviewed, UniProtKB/Swiss-Prot Q91554 (ARGN2_XENLA)

Names and origin

Protein names

Recommended name:
    Arginase, non-hepatic 2
    EC=3.5.3.1

Gene names

Name:	arg2-B
Synonyms:	arg2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	As well as its role in the urea cycle, may be involved in tissue remodeling.
Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Subunit structure	Homotrimer By similarity.
Tissue specificity	Expressed at differing tadpole stages in tail, intestine, hindlimb and trunk region. Strongest in tadpole tail.
Developmental stage	First detected in neurula (stage 16/17). Highest levels in whole tadpole found around stage 47/48. In the intestine, increased levels are found during metamorphosis (stages 58-64) and in the hindlimb, expressed at low levels during metamorphosis until stage 66 when levels dramatically increase. In the tail, a constant high level of expression is found throughout metamorphosis.
Induction	By thyroid hormone (T3).
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism Urea cycle
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arginase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Arginase, non-hepatic 2		PRO_0000173700
Regions
	Region	147 – 151	5	Substrate binding By similarity
	Region	158 – 160	3	Substrate binding By similarity
Sites
	Metal binding	122	1	Manganese 1 By similarity
	Metal binding	145	1	Manganese 1 By similarity
	Metal binding	145	1	Manganese 2 By similarity
	Metal binding	147	1	Manganese 2 By similarity
	Metal binding	149	1	Manganese 1 By similarity
	Metal binding	253	1	Manganese 1 By similarity
	Metal binding	253	1	Manganese 2 By similarity
	Metal binding	255	1	Manganese 2 By similarity
	Binding site	204	1	Substrate By similarity
	Binding site	298	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91554-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 44A4E61915DD0833	FASTA	360	39,155
	10 20 30 40 50 60 MSIRSNFVRL LKKQVSIIKL QKKCSHSVAV IGAPFSKGQK RRGVEHGPAA IRSAGLIERL 70 80 90 100 110 120 SNLGCNVCDF GDLHFSKVPN DELYNSIVKH PRTVGLACKV LAEEVSKAVG AGHTCVTLGG 130 140 150 160 170 180 DHSLAFGSIT GHAQQCPDLC VIWVDAHADI NTPLTTPSGN LHGQPVSFLL RELQDKVPPI 190 200 210 220 230 240 PGFSWAKPCL SKSDIVYIGL RDLDPAEQFI LKNYNISYYS MRHIDCMGIK KVMEKTFDQL 250 260 270 280 290 300 LGRRDRPIHL SFDIDAFDPA LAPATGTPVI GGLTYREGVY ITEEIHNTGM LSAVDLVEVN 310 320 330 340 350 360 PVLAATSEEV KATANLAVDV IASCFGQTRE GAHTRADTII DVLPTPSTSY ESDNEEQVRI

References

[1]	"Thyroid hormone-dependent differential regulation of multiple arginase genes during amphibian metamorphosis." Patterton D., Shi Y.-B. J. Biol. Chem. 269:25328-25334(1994) [PubMed: 7929226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U08407 mRNA. Translation: AAA56892.1.
PIR	I51664.
RefSeq	NP_001079511.1.
UniGene	Xl.892
3D structure databases
HSSP	HSSP built from PDB template 1PQ3 based on UniProtKB P78540.
SMR	Q91554. Positions 26-331.
ModBase	Search...
Genome annotation databases
GeneID	379198.
KEGG	xla:379198.
Organism-specific databases
CTD	379198.
Phylogenomic databases
HOVERGEN	Q91554.
Enzyme and pathway databases
BRENDA	3.5.3.1. 648.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGN2_XENLA

Accession

Primary (citable) accession number: Q91554

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 1, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project