Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Stonustoxin subunit beta

Gene
N/A
Organism
Synanceia horrida (Estuarine stonefish) (Scorpaena horrida)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This lethal (towards mice) toxin induces hemolytic activities due to its ability to form pores in the cell membrane, elicits potent hypotension which is endothelium-dependent and appears to be mediated by the nitric oxide pathway and activation of potassium channels, displays edema-inducing activities, increases vascular permeability, induces endothelium-dependent vasorelaxation of isolated rat aorta, induces platelet aggregation, is myotoxic and interferes irreversibly with neuromuscular function.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Myotoxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Keywords - Biological processi

Cytolysis, Hemolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Stonustoxin subunit beta
Short name:
SNTX subunit beta
OrganismiSynanceia horrida (Estuarine stonefish) (Scorpaena horrida)
Taxonomic identifieri13279 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataEupercariaPerciformesScorpaenoideiSynanceiidaeSynanceiinaeSynanceia

Subcellular locationi

  • Secreted

  • Note: Secreted into the venom gland lumen. The secretion is proved by the fact that the complete sequence showed below is found in the venom gland's lumen, although no signal peptide has been found. This protein may follow a novel secretion pathway. It has been reported that venom-secreting cells of stonefishes do not possess Golgi apparatus and rough endoplasmic reticulum.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 of stonustoxin is 0.017 mg/kg by intravenous injection.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 700699Stonustoxin subunit betaPRO_0000221556Add
BLAST

Post-translational modificationi

Five intrachain disulfide linkages are present in the heterodimer. No interchain disulfide bound links the two subunits.
Not glycosylated.

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer of alpha and beta subunits.

Structurei

Secondary structure

1
700
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 94Combined sources
Beta strandi47 – 5812Combined sources
Helixi62 – 687Combined sources
Helixi73 – 808Combined sources
Helixi90 – 945Combined sources
Beta strandi104 – 11613Combined sources
Beta strandi140 – 15718Combined sources
Helixi165 – 17814Combined sources
Helixi195 – 2006Combined sources
Beta strandi203 – 2119Combined sources
Helixi220 – 22910Combined sources
Turni230 – 2323Combined sources
Beta strandi240 – 2489Combined sources
Helixi265 – 28925Combined sources
Helixi292 – 2965Combined sources
Helixi298 – 32730Combined sources
Helixi337 – 34812Combined sources
Helixi353 – 37826Combined sources
Helixi388 – 3958Combined sources
Beta strandi401 – 4088Combined sources
Helixi416 – 42611Combined sources
Helixi439 – 4413Combined sources
Helixi443 – 4453Combined sources
Helixi448 – 46619Combined sources
Beta strandi469 – 47810Combined sources
Beta strandi486 – 4938Combined sources
Beta strandi496 – 5016Combined sources
Helixi510 – 5123Combined sources
Helixi516 – 5205Combined sources
Turni530 – 5323Combined sources
Beta strandi538 – 5403Combined sources
Turni541 – 5444Combined sources
Beta strandi545 – 5473Combined sources
Beta strandi562 – 5643Combined sources
Beta strandi566 – 5705Combined sources
Beta strandi575 – 5839Combined sources
Beta strandi588 – 5925Combined sources
Helixi603 – 6053Combined sources
Beta strandi609 – 6179Combined sources
Beta strandi625 – 6284Combined sources
Beta strandi631 – 6344Combined sources
Beta strandi638 – 6414Combined sources
Beta strandi643 – 6508Combined sources
Turni651 – 6544Combined sources
Beta strandi655 – 6628Combined sources
Beta strandi665 – 6739Combined sources
Beta strandi680 – 6856Combined sources
Beta strandi696 – 6994Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WVMX-ray3.10B1-700[»]
ProteinModelPortaliQ91453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini506 – 700195B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SNTX/VTX toxin family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG079636.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSDILVVAA LGRPFTLGML YDARNDKLIP GFTLWEDEVI EESTLESSQP
60 70 80 90 100
SSAFEIIASD STDDKSSLMD IEASLKASFL GGLVEVGGSA KYLNNQKKFK
110 120 130 140 150
NQSRVTLQYK ATTSFKQLMT NLGTKHVEYS ELFENIQATH VVIGILYGAN
160 170 180 190 200
AFFVFDSNKV DSTNVQEIQG QMEAVIKKIP SVEISGKASV QLTGEETDIT
210 220 230 240 250
NSFSCEFHGD FFLTTNPTTF EDAVKTYQQL PQMMGKDNAV PMTVWLVPMV
260 270 280 290 300
NFYSEAPQLM ADSSTPILRK VRNTLEAIVQ VQMRCNDALD DPTVNLFTEV
310 320 330 340 350
QKKLSDFQKI CDDHMSKLQA TIAKKLFAIR SGDEDESALL NLFEENLQSP
360 370 380 390 400
FNIESLNMWM EFEEREINVL RSCMDILTKA KPKVIFNQGV LFKGLYDSKV
410 420 430 440 450
KHALCYVFTN VTKNDVFLNV LNEFLDSPQS RPKKLRPSPK DYWYSYDDIP
460 470 480 490 500
ETMREKAYLF RNLAKEMNNR CVHFFVTAIH NPKQEGAGIH YYRESIQIID
510 520 530 540 550
EFTKPYMPGV ESIKDRRELQ WYDCELTLDP ETAHQVLTLS EGNKKAVSGN
560 570 580 590 600
TKSPTDHLEK FSHFQQVMCT KGLSGRHYWE LEWSGYVGAG VTYKGIGRKT
610 620 630 640 650
STSDSSLGKN EKSWLFEYST KSGYQQIHNS KKTRVTVSST GFKLLGVYLD
660 670 680 690 700
WPAGTLSFYM VNKAWVTHLH TFHTKFNEAV YPAFLIGDAQ QKVNGQIKLL
Length:700
Mass (Da):79,425
Last modified:January 23, 2007 - v3
Checksum:i53445821AA3A46C1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti506 – 5061Y → P (PubMed:7725338).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32516 mRNA. Translation: AAC60021.1.
PIRiI51333. A61527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32516 mRNA. Translation: AAC60021.1.
PIRiI51333. A61527.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WVMX-ray3.10B1-700[»]
ProteinModelPortaliQ91453.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG079636.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTXB_SYNHO
AccessioniPrimary (citable) accession number: Q91453
Secondary accession number(s): Q98986
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.