Q91348 (F26L_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase Short name=6PF-2-K/Fru-2,6-P2ase Short name=PFK/FBPase Alternative name(s): 6PF-2-K/Fru-2,6-P2ase liver isozyme Including the following 2 domains: 6-phosphofructo-2-kinase EC=2.7.1.105 Fructose-2,6-bisphosphatase EC=3.1.3.46
Organism	Gallus gallus (Chicken)
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	Phosphorylation results in inhibition of the kinase activity By similarity.
Subunit structure	Homodimer By similarity.
Tissue specificity	Liver.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase		PRO_0000179972
Regions
	Nucleotide binding	47 – 54	8	ATP Potential
	Region	1 – 249	249	6-phosphofructo-2-kinase
	Region	250 – 469	220	Fructose-2,6-bisphosphatase
Sites
	Active site	130	1	Potential
	Active site	160	1	Potential
	Active site	258	1	Tele-phosphohistidine intermediate
	Active site	327	1	Potential
	Active site	392	1	Proton donor By similarity
	Binding site	104	1	Fructose-6-phosphate By similarity
	Binding site	195	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	31	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91348 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: DCDBFB5D392033F8	FASTA	470	54,404
	10 20 30 40 50 60 MAAVASGQLT QNPLQKVWVP LSLHRLRRRG STVPQFTNCP TMVILVGLRR PGKTYISRKL 70 80 90 100 110 120 TRYLNWIGMP TRVFNVGQYR REAVQSYKNY EFFRHDNEEA MQIRRQCALA ALQDVRTYLS 130 140 150 160 170 180 SEEGQVAVFD ATNTTRERRA LIMQFARENG YKVLFVESIC DDPAIIEENI KQVKLSSPDY 190 200 210 220 230 240 KGCTPEEAVA DFLQRIECYK ATYEPLDEQL DSGLSYIKIF DVGVRYLANR VQGHVQSRTV 250 260 270 280 290 300 YYLMNTHVTP RAIYLSRHGE SQLNLKGRIG GDAGLSTRGR QYAQALAEFI RSQSIRELKV 310 320 330 340 350 360 WTSHMKRTIE TAEALGVPYE QWKALNEIDA GVCEEMTYEE IQERYPEEFA LRDQDKYRYR 370 380 390 400 410 420 YPKGESYEDL VQRLEPVIME LERQENVLVI CHQAVMRCLL AYFLDKSSEE LPYLRCPLHT 430 440 450 460 470 VLKLTPVAYG CEVESIFLNV EAVNTHRERP QNVDISRPPA EALVTVPEHY

References

[1]	"Isolation of a cDNA for chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase." Li L., Lange A.J., Pilkis S.J. Biochem. Biophys. Res. Commun. 190:397-405(1993) [PubMed: 7916593] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	Yang Q.H., Dong M.Q., Li L. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 184-186 AND 365-366.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF146428 mRNA. Translation: AAD37721.1.
IPI	IPI00601795.
PIR	JC1470.
RefSeq	NP_001025755.1. NM_001030584.1.
UniGene	Gga.17198.
3D structure databases
ProteinModelPortal	Q91348.
SMR	Q91348. Positions 8-470.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	415906.
KEGG	gga:415906.
Organism-specific databases
CTD	5210.
Phylogenomic databases
eggNOG	veNOG10157.
HOVERGEN	HBG005628.
PhylomeDB	Q91348.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR013078. His_Pase_superF_clade-1. IPR001345. PG/BPGM_mutase_AS. [Graphical view]
KO	K01103.
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. His_Phos_1. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
SMART	SM00855. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F26L_CHICK

Accession

Primary (citable) accession number: Q91348
Secondary accession number(s): Q9PWA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 16, 2004
Last modified:	November 16, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program