Reviewed, UniProtKB/Swiss-Prot Q91309 (F26_RANCA)

Names and origin

Protein names	Recommended name:     6PF-2-K/Fru-2,6-P2ASE liver/muscle isozymes Including the following 2 domains:     1- Recommended name:             6-phosphofructo-2-kinase               EC=2.7.1.105     2- Recommended name:             Fructose-2,6-bisphosphatase               EC=3.1.3.46
Organism	Rana catesbeiana (Bull frog)
Taxonomic identifier	8400 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Rana › Aquarana

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	Phosphorylation results in inhibition of the kinase activity By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Liver (identifier: Q91309-1)

Also known as: L-type;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Muscle (identifier: Q91309-2)

Also known as: M-type;

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: MADRLRELTQTRLQKIWIPHQCDRLQQRRGS → MEGNYKLLEDKASRIPA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	6PF-2-K/Fru-2,6-P2ASE liver/muscle isozymes		PRO_0000179973
Regions
	Nucleotide binding	47 – 54	8	ATP Potential
	Region	1 – 249	249	6-phosphofructo-2-kinase
	Region	250 – 470	221	Fructose-2,6-bisphosphatase
Sites
	Active site	130	1	Potential
	Active site	160	1	Potential
	Active site	258	1	Tele-phosphohistidine intermediate
	Active site	327	1	Potential
	Active site	392	1	Proton donor By similarity
	Binding site	104	1	Fructose-6-phosphate By similarity
	Binding site	195	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	31	1	Phosphoserine; by PKA By similarity
Natural variations
	Alternative sequence	1 – 31	31	MADRL…QRRGS → MEGNYKLLEDKASRIPA in isoform Muscle.		VSP_004685

Sequences

	Sequence		Length	Mass (Da)
	Isoform Liver (L-type) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 53112EF3B886D310	FASTA	470	54,933
	10 20 30 40 50 60 MADRLRELTQ TRLQKIWIPH QCDRLQQRRG SSIPQFTNSP TMIVMVGLPA RGKTYISKKL 70 80 90 100 110 120 TRYLNWIGTP TKVFNVGQYR RDATQSYNNY QFFRADNQEA MKIRKQCALH ALKDVHTYLS 130 140 150 160 170 180 REEGHVAVFD ATNTTRERRS VILQFAKERG YKVFFIESIC DDPDIIAENI TQVKLSSPDY 190 200 210 220 230 240 TGCDREKVLE DFLKRIDCYQ MNYEPLHDDL DSSLSYIKIF NVGSRYLVNR VQDHIQSRAV 250 260 270 280 290 300 YYLMNIHVTP RSIYLSRHGE SELNLLGRIG GDSGLSVRGK QYAHELGNFI KSQQIPDLKV 310 320 330 340 350 360 WTSHMKRTIQ TAEALHVPYE QWKALNEIDA GVCEEMTYEE IQDHFPEEFA LRDQDKYRYR 370 380 390 400 410 420 YPKGESYEDI VQRLEPVIME LERQENVLVI CHQAVMRCLL AYFLDKTAEE LPYLKCPLHT 430 440 450 460 470 VLKLTPVAYG CKVESIYLNI EAVNTHREKP LNVEVSRDPE EALDTVPEHF
	Isoform Muscle (M-type). Checksum: 82472C3EC856AEC5	FASTA	456	53,033
	10 20 30 40 50 60 MEGNYKLLED KASRIPASIP QFTNSPTMIV MVGLPARGKT YISKKLTRYL NWIGTPTKVF 70 80 90 100 110 120 NVGQYRRDAT QSYNNYQFFR ADNQEAMKIR KQCALHALKD VHTYLSREEG HVAVFDATNT 130 140 150 160 170 180 TRERRSVILQ FAKERGYKVF FIESICDDPD IIAENITQVK LSSPDYTGCD REKVLEDFLK 190 200 210 220 230 240 RIDCYQMNYE PLHDDLDSSL SYIKIFNVGS RYLVNRVQDH IQSRAVYYLM NIHVTPRSIY 250 260 270 280 290 300 LSRHGESELN LLGRIGGDSG LSVRGKQYAH ELGNFIKSQQ IPDLKVWTSH MKRTIQTAEA 310 320 330 340 350 360 LHVPYEQWKA LNEIDAGVCE EMTYEEIQDH FPEEFALRDQ DKYRYRYPKG ESYEDIVQRL 370 380 390 400 410 420 EPVIMELERQ ENVLVICHQA VMRCLLAYFL DKTAEELPYL KCPLHTVLKL TPVAYGCKVE 430 440 450 SIYLNIEAVN THREKPLNVE VSRDPEEALD TVPEHF

References

[1]

"Cloning of cDNAs for fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase from frog skeletal muscle and liver, and their expression in skeletal muscle." Sakai A., Watanabe F., Furuya E. Biochem. Biophys. Res. Commun. 198:1099-1106(1994) [PubMed: 7509597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LIVER AND MUSCLE). Tissue: Liver and Muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D25223 mRNA. Translation: BAA04952.1. D25222 mRNA. Translation: BAA04951.1.
PIR	JC2064.
3D structure databases
HSSP	HSSP built from PDB template 1C80 based on UniProtKB P07953.
SMR	Q91309. Positions 40-469.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q91309.
Enzyme and pathway databases
BRENDA	2.7.1.105. 829. 3.1.3.46. 829.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR001345. PG/BPGM_mutase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F26_RANCA

Accession

Primary (citable) accession number: Q91309
Secondary accession number(s): Q91310

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)