Q912Z9 (X_BDV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
X protein

Alternative name(s):
p10

Gene names

Name:

P/X

Organism

Borna disease virus (BDV)

Taxonomic identifier

12455 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Bornaviridae › Bornavirus

Virus host

Struthio camelus (Ostrich) [TaxID: 8801]
Bradypodidae (three-fingered sloths) [TaxID: 9352]
Lemur variegatus (Ruffed lemur) (Varecia variegata) [TaxID: 9455]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Equidae (horses) [TaxID: 9788]
Lama glama (Llama) [TaxID: 9844]
Cervidae (deer) [TaxID: 9850]
Bos taurus (Bovine) [TaxID: 9913]
Capra hircus (Goat) [TaxID: 9925]
Ovis aries (Sheep) [TaxID: 9940]
Oryctolagus cuniculus (Rabbit) [TaxID: 9986]
Lama guanicoe pacos (Alpaca) (Lama pacos) [TaxID: 30538]
Hexaprotodon liberiensis (Pygmy hippopotamus) [TaxID: 56798]
Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew) [TaxID: 109474]

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role as a cofactor of the viral polymerase.
Subunit structure	Interacts with P and N proteins. These interactions presumably promote nuclear targeting of the X protein in infected cells. Ref.3
Subcellular location	Host nucleus. Host cytoplasm. Note: Cytoplasmic if expressed without other viral proteins.
Post-translational modification	Phosphorylated.
Miscellaneous	The P/X gene has 2 overlapping open reading frames. One encodes the P protein and the other the X protein. The p24, X and p16 proteins are produced by ribosomal leaky scanning.

Ontologies

Keywords
Cellular component	Host cytoplasm Host nucleus
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 87	87	X protein		PRO_0000079208
Natural variations
	Natural variant	20	1	A → G in strain: Isolate CRNP5, Isolate CRP3B and isolate CRP3A.
	Natural variant	42	1	T → I in strain: Isolate CRNP5, Isolate CRP3B and isolate CRP3A.
	Natural variant	50	1	G → D in strain: Isolate CRNP5, Isolate CRP3B and isolate CRP3A.
	Natural variant	59	1	S → G in strain: Isolate CRNP5, Isolate CRP3B and isolate CRP3A.
	Natural variant	86	1	V → I in strain: Isolate CRNP5, Isolate CRP3B and isolate CRP3A.
Experimental info
	Mutagenesis	2 – 3	2	SS → AA: No effect on interaction with P protein.
	Mutagenesis	4 – 5	2	DL → AA: No effect on interaction with P protein.
	Mutagenesis	6 – 7	2	RL → AA: No effect on interaction with P protein.
	Mutagenesis	7 – 10	4	LTLL → ATAA: Complete loss of interaction with P protein. Ref.4
	Mutagenesis	8 – 9	2	TL → AA: Complete loss of interaction with P protein.
	Mutagenesis	10 – 11	2	LE → AA: Complete loss of interaction with P protein.
	Mutagenesis	12 – 13	2	LV → AA: Partial loss of interaction with P protein.
	Mutagenesis	14 – 15	2	RR → AA: Complete loss of interaction with P protein.
	Mutagenesis	16 – 17	2	LN → AA: No effect on interaction with P protein.
	Mutagenesis	18 – 19	2	GN → AA: No effect on interaction with P protein.
	Mutagenesis	21	1	T → A: No effect on interaction with P protein. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q912Z9 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 936743D28F9719FD	FASTA	87	9,386
	10 20 30 40 50 60 MSSDLRLTLL ELVRRLNGNA TIESGRLPGG RRRSPDTTTG TTGVTKTTEG PKECIDPTSR 70 80 PAPEGPQEEP LHDLRPRPAN RKGAAVE

References

[1]	"Conservation of coding potential and terminal sequences in four different isolates of Borna disease virus." Pleschka S., Staeheli P., Kolodziejek J., Richt J.A., Nowotny N., Schwemmle M. J. Gen. Virol. 82:2681-2690(2001) [PubMed: 11602780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: V/FR.
[2]	"Enhanced neurovirulence of borna disease virus variants associated with nucleotide changes in the glycoprotein and L polymerase genes." Nishino Y., Kobasa D., Rubin S.A., Pletnikov M.V., Carbone K.M. J. Virol. 76:8650-8658(2002) [PubMed: 12163584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate CRNP5, Isolate CRP3A and Isolate CRP3B.
[3]	"Characterization of the P protein-binding domain on the 10-kilodalton protein of Borna disease virus." Malik T.H., Kishi M., Lai P.K. J. Virol. 74:3413-3417(2000) [PubMed: 10708460] [Abstract] Cited for: INTERACTION WITH P PROTEIN.
[4]	"A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoproteins." Wolff T., Pfleger R., Wehner T., Reinhardt J., Richt J.A. J. Gen. Virol. 81:939-947(2000) [PubMed: 10725419] [Abstract] Cited for: MUTAGENESIS OF 2-SER-SER-3; 4-ASP-LEU-5; 6-ARG-LEU-7; 7-LEU--LEU-10; 8-THR-LEU-9; 10-LEU-GLU-11; 12-LEU-GLU-13; 14-ARG-ARG-15; 16-LEU-ASN-17; 18-GLY-ASN-19 AND THR-21. Strain: He/80.
[5]	"Borna disease virus and infection in humans." Ikuta K., Ibrahim M.S., Kobayashi T., Tomonaga K. Front. Biosci. 7:470-495(2002) [PubMed: 11815287] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ311521 Genomic RNA. Translation: CAC70635.1. AY114161 Genomic RNA. Translation: AAM68135.1. AY114162 Genomic RNA. Translation: AAM68141.1. AY114163 Genomic RNA. Translation: AAM68147.1.
RefSeq	YP_009031.1. NC_001607.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2808004.
Family and domain databases
InterPro	IPR009485. BDV_P10. [Graphical view]
Pfam	PF06515. BDV_P10. 1 hit. [Graphical view]
ProDom	PD057343. BDV_P10. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

X_BDV

Accession

Primary (citable) accession number: Q912Z9
Secondary accession number(s): Q8JJK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program