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Q912W1 (REP_CACV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication-associated protein

EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3
Alternative name(s):
ATP-dependent helicase Rep
RepP
Gene names
Name:Rep
ORF Names:ORF1
OrganismCanary circovirus (CaCV) [Complete proteome]
Taxonomic identifier142661 [NCBI]
Taxonomic lineageVirusesssDNA virusesCircoviridaeCircovirus
Virus hostSerinus [TaxID: 9134]

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese By similarity.

Subunit structure

Interacts with the capsid protein; this interaction relocates Rep into the nucleus By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the nanoviruses/circoviruses replication-associated protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Replication-associated protein
PRO_0000319863

Regions

Nucleotide binding165 – 1728ATP By similarity
Motif4 – 1310Nuclear localization signal Potential
Motif14 – 174RCR-1 By similarity
Motif51 – 566RCR-2 By similarity
Motif60 – 8021Nuclear localization signal Potential
Motif89 – 924RCR-3 By similarity

Sites

Active site891For DNA cleavage activity By similarity
Metal binding431Divalent metal cation Potential
Metal binding511Divalent metal cation Potential
Metal binding931Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q912W1 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 98095B2736B6B4C8

FASTA29033,372
        10         20         30         40         50         60 
MAPVRAAAAK RWCFTLNNYT AEEEAKVRAL LPGEFHFAIC GKERGEQGTP HLQGFLHFKK 

        70         80         90        100        110        120 
KQRLSALKKL LARAHWEKAR GSDHDNEEYC SKENDVILTI GEPVQGNRSD LAGAVAAVKA 

       130        140        150        160        170        180 
GRRMVDIARE FSEIYVKYGR GLRDLALMIG QKPRDFKTEV VVITGPSGVG KSRLASEMEG 

       190        200        210        220        230        240 
SKFYKMKGDW WDGYSNEDIV IMDDFYGWLP FCEMLRLMDR YPHKVPVKGS YVEFTSKKIV 

       250        260        270        280        290 
ITSNTHPESW YCPDKCYLPA LFRRINKWMY WDGLRFEDVP DAMKKHPINY 

« Hide

References

[1]"Nucleotide sequence-based identification of a novel circovirus of canaries."
Todd D., Weston J., Ball N.W., Borghmans B.J., Smyth J.A., Gelmini L., Lavazza A.
Avian Pathol. 30:321-325(2001)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ301633 Genomic DNA. Translation: CAC44748.2.
RefSeqNP_573442.1. NC_003410.1.

3D structure databases

ProteinModelPortalQ912W1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID935131.

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameREP_CACV
AccessionPrimary (citable) accession number: Q912W1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families