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Q912W1

- REP_CACV

UniProt

Q912W1 - REP_CACV

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Protein

Replication-associated protein

Gene

Rep

Organism
Canary circovirus (CaCV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Divalent metal cations, possibly magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Divalent metal cationSequence Analysis
Metal bindingi51 – 511Divalent metal cationSequence Analysis
Active sitei89 – 891For DNA cleavage activityBy similarity
Metal bindingi93 – 931Divalent metal cationSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1728ATPBy similarity

GO - Molecular functioni

  1. ATPase activity, uncoupled Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. DNA binding Source: UniProtKB-KW
  4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. nucleotidyltransferase activity Source: UniProtKB-KW
  7. RNA binding Source: InterPro
  8. RNA helicase activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein-DNA covalent cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
Alternative name(s):
ATP-dependent helicase Rep
RepP
Gene namesi
Name:Rep
ORF Names:ORF1
OrganismiCanary circovirus (CaCV)
Taxonomic identifieri142661 [NCBI]
Taxonomic lineageiVirusesssDNA virusesCircoviridaeCircovirus
Virus hostiSerinus [TaxID: 9134]
ProteomesiUP000007621: Genome

Subcellular locationi

Host nucleus Curated

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Replication-associated proteinPRO_0000319863Add
BLAST

Keywords - PTMi

Covalent protein-DNA linkage

Interactioni

Subunit structurei

Interacts with the capsid protein; this interaction relocates Rep into the nucleus.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ912W1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi4 – 1310Nuclear localization signalSequence Analysis
Motifi14 – 174RCR-1By similarity
Motifi51 – 566RCR-2By similarity
Motifi60 – 8021Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi89 – 924RCR-3By similarity

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Family and domain databases

InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamiPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q912W1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPVRAAAAK RWCFTLNNYT AEEEAKVRAL LPGEFHFAIC GKERGEQGTP
60 70 80 90 100
HLQGFLHFKK KQRLSALKKL LARAHWEKAR GSDHDNEEYC SKENDVILTI
110 120 130 140 150
GEPVQGNRSD LAGAVAAVKA GRRMVDIARE FSEIYVKYGR GLRDLALMIG
160 170 180 190 200
QKPRDFKTEV VVITGPSGVG KSRLASEMEG SKFYKMKGDW WDGYSNEDIV
210 220 230 240 250
IMDDFYGWLP FCEMLRLMDR YPHKVPVKGS YVEFTSKKIV ITSNTHPESW
260 270 280 290
YCPDKCYLPA LFRRINKWMY WDGLRFEDVP DAMKKHPINY
Length:290
Mass (Da):33,372
Last modified:June 1, 2002 - v2
Checksum:i98095B2736B6B4C8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ301633 Genomic DNA. Translation: CAC44748.2.
RefSeqiNP_573442.1. NC_003410.1.

Genome annotation databases

GeneIDi935131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ301633 Genomic DNA. Translation: CAC44748.2 .
RefSeqi NP_573442.1. NC_003410.1.

3D structure databases

ProteinModelPortali Q912W1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 935131.

Family and domain databases

InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view ]
Pfami PF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence-based identification of a novel circovirus of canaries."
    Todd D., Weston J., Ball N.W., Borghmans B.J., Smyth J.A., Gelmini L., Lavazza A.
    Avian Pathol. 30:321-325(2001)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiREP_CACV
AccessioniPrimary (citable) accession number: Q912W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3