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Q912W1

- REP_CACV

UniProt

Q912W1 - REP_CACV

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Protein

Replication-associated protein

Gene
Rep, ORF1
Organism
Canary circovirus (CaCV)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Divalent metal cations, possibly magnesium or manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Divalent metal cation Reviewed prediction
Metal bindingi51 – 511Divalent metal cation Reviewed prediction
Active sitei89 – 891For DNA cleavage activity By similarity
Metal bindingi93 – 931Divalent metal cation Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1728ATP By similarity

GO - Molecular functioni

  1. ATPase activity, uncoupled Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. DNA binding Source: UniProtKB-KW
  4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. nucleotidyltransferase activity Source: UniProtKB-KW
  7. RNA binding Source: InterPro
  8. RNA helicase activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein-DNA covalent cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
Alternative name(s):
ATP-dependent helicase Rep
RepP
Gene namesi
Name:Rep
ORF Names:ORF1
OrganismiCanary circovirus (CaCV)
Taxonomic identifieri142661 [NCBI]
Taxonomic lineageiVirusesssDNA virusesCircoviridaeCircovirus
Virus hostiSerinus [TaxID: 9134]
ProteomesiUP000007621: Genome

Subcellular locationi

Host nucleus Reviewed prediction

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Replication-associated proteinPRO_0000319863Add
BLAST

Keywords - PTMi

Covalent protein-DNA linkage

Interactioni

Subunit structurei

Interacts with the capsid protein; this interaction relocates Rep into the nucleus By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ912W1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi4 – 1310Nuclear localization signal Reviewed prediction
Motifi14 – 174RCR-1 By similarity
Motifi51 – 566RCR-2 By similarity
Motifi60 – 8021Nuclear localization signal Reviewed predictionAdd
BLAST
Motifi89 – 924RCR-3 By similarity

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similaritiesi

Family and domain databases

InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view]
PfamiPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q912W1-1 [UniParc]FASTAAdd to Basket

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MAPVRAAAAK RWCFTLNNYT AEEEAKVRAL LPGEFHFAIC GKERGEQGTP    50
HLQGFLHFKK KQRLSALKKL LARAHWEKAR GSDHDNEEYC SKENDVILTI 100
GEPVQGNRSD LAGAVAAVKA GRRMVDIARE FSEIYVKYGR GLRDLALMIG 150
QKPRDFKTEV VVITGPSGVG KSRLASEMEG SKFYKMKGDW WDGYSNEDIV 200
IMDDFYGWLP FCEMLRLMDR YPHKVPVKGS YVEFTSKKIV ITSNTHPESW 250
YCPDKCYLPA LFRRINKWMY WDGLRFEDVP DAMKKHPINY 290
Length:290
Mass (Da):33,372
Last modified:June 1, 2002 - v2
Checksum:i98095B2736B6B4C8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ301633 Genomic DNA. Translation: CAC44748.2.
RefSeqiNP_573442.1. NC_003410.1.

Genome annotation databases

GeneIDi935131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ301633 Genomic DNA. Translation: CAC44748.2 .
RefSeqi NP_573442.1. NC_003410.1.

3D structure databases

ProteinModelPortali Q912W1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 935131.

Family and domain databases

InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR027417. P-loop_NTPase.
IPR003365. Viral_rep_N.
[Graphical view ]
Pfami PF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence-based identification of a novel circovirus of canaries."
    Todd D., Weston J., Ball N.W., Borghmans B.J., Smyth J.A., Gelmini L., Lavazza A.
    Avian Pathol. 30:321-325(2001)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiREP_CACV
AccessioniPrimary (citable) accession number: Q912W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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