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Q912W1

- REP_CACV

UniProt

Q912W1 - REP_CACV

Protein

Replication-associated protein

Gene

Rep

Organism
Canary circovirus (CaCV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. ATPase activity is probably carried by the isoform Rep By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Divalent metal cations, possibly magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi43 – 431Divalent metal cationSequence Analysis
    Metal bindingi51 – 511Divalent metal cationSequence Analysis
    Active sitei89 – 891For DNA cleavage activityBy similarity
    Metal bindingi93 – 931Divalent metal cationSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi165 – 1728ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity, uncoupled Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. DNA binding Source: UniProtKB-KW
    4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. metal ion binding Source: UniProtKB-KW
    6. nucleotidyltransferase activity Source: UniProtKB-KW
    7. RNA binding Source: InterPro
    8. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein-DNA covalent cross-linking Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication-associated protein (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
    Alternative name(s):
    ATP-dependent helicase Rep
    RepP
    Gene namesi
    Name:Rep
    ORF Names:ORF1
    OrganismiCanary circovirus (CaCV)
    Taxonomic identifieri142661 [NCBI]
    Taxonomic lineageiVirusesssDNA virusesCircoviridaeCircovirus
    Virus hostiSerinus [TaxID: 9134]
    ProteomesiUP000007621: Genome

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290Replication-associated proteinPRO_0000319863Add
    BLAST

    Keywords - PTMi

    Covalent protein-DNA linkage

    Interactioni

    Subunit structurei

    Interacts with the capsid protein; this interaction relocates Rep into the nucleus.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ912W1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi4 – 1310Nuclear localization signalSequence Analysis
    Motifi14 – 174RCR-1By similarity
    Motifi51 – 566RCR-2By similarity
    Motifi60 – 8021Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi89 – 924RCR-3By similarity

    Domaini

    There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR027417. P-loop_NTPase.
    IPR003365. Viral_rep_N.
    [Graphical view]
    PfamiPF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q912W1-1 [UniParc]FASTAAdd to Basket

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    MAPVRAAAAK RWCFTLNNYT AEEEAKVRAL LPGEFHFAIC GKERGEQGTP    50
    HLQGFLHFKK KQRLSALKKL LARAHWEKAR GSDHDNEEYC SKENDVILTI 100
    GEPVQGNRSD LAGAVAAVKA GRRMVDIARE FSEIYVKYGR GLRDLALMIG 150
    QKPRDFKTEV VVITGPSGVG KSRLASEMEG SKFYKMKGDW WDGYSNEDIV 200
    IMDDFYGWLP FCEMLRLMDR YPHKVPVKGS YVEFTSKKIV ITSNTHPESW 250
    YCPDKCYLPA LFRRINKWMY WDGLRFEDVP DAMKKHPINY 290
    Length:290
    Mass (Da):33,372
    Last modified:June 1, 2002 - v2
    Checksum:i98095B2736B6B4C8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ301633 Genomic DNA. Translation: CAC44748.2.
    RefSeqiNP_573442.1. NC_003410.1.

    Genome annotation databases

    GeneIDi935131.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ301633 Genomic DNA. Translation: CAC44748.2 .
    RefSeqi NP_573442.1. NC_003410.1.

    3D structure databases

    ProteinModelPortali Q912W1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 935131.

    Family and domain databases

    InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR027417. P-loop_NTPase.
    IPR003365. Viral_rep_N.
    [Graphical view ]
    Pfami PF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence-based identification of a novel circovirus of canaries."
      Todd D., Weston J., Ball N.W., Borghmans B.J., Smyth J.A., Gelmini L., Lavazza A.
      Avian Pathol. 30:321-325(2001)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiREP_CACV
    AccessioniPrimary (citable) accession number: Q912W1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 46 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3